Biochemical Characteristics and Antimicrobials Susceptibility of Salmonella gallinarum Isolated in Korea

Ki-seuk Kim,Young-ju Lee,Yong-kuk Kwon,Ryun-bin Tak 대한수의학회 2003 Journal of Veterinary Science Vol.4 No.2

Characteristics and Antimicrobials Susceptibility of Salmonella gallinarum Isolated in KoreaYoung-ju Lee, Ki-seuk Kim1,*, Yong-kuk Kwon and Ryun-bin Tak1Avian Disease Division, National Veterinary Research and Quarantine Service, Anyang 430-824, Korea1College of Veterinary Medicine, Kyungpook National University, Daegu 702-701, KoreaReceived February 13, 2003 / Accepted April 30, 2003J. Vet. Sci. (2003), 4(2), 161-166JOURNAL OFVeterinaryScience*Corresponding author: Ki-seuk Kim College of Veterinary Medicine, Kyungpook National University, Daegu 702-701, Korea Tel: 82-53-950-5962, Fax: 82-53-950-5955 E-mail: kimkiseuk@knu.ac.kr

Large cell acanthoma의 임상 및 병리 조직학적 소견과 Ki-67 발현 양상

정해홍(Hae Hong Jeong),서기석(Ki Seuk Suh),김상태(Sang Tae Kim) 대한피부과학회 1999 대한피부과학회지 Vol.37 No.9

N/A Background : 1970, Pinkus described large cell acanthoma(LCA) as a benign epidermal neoplasm which clinically resembles a actinic keratosis or seborrheic keratosis. However, the entity of this disease is still unclear. Objective : The purpose of this study was to find out if LCA is a distinctive entity by investigating its clinical features, histopathologic features and Ki-67 expreesion. Methods : 20 LCA samples including normal tissue were analyzed after investigating their clinicopathologic features and immunohistochemical analysis of Ki-67 expression which was performed on the formalin-fixed, praffin-embedded tissue section. The assessment of immunohistochemical staining was based on the growth fraction(GF), defined as the number of Ki-67 positive cells divided by the total number of tumor cells counted, and was expressed in a percentage. Results : 1. LCA was usually presented as a well demarcated, brown or light brown, 5 to 30 mm, round, flat to hyperkeratotic scaly patch and plaque. It predominantly affected female(5.6 female : 1 males) againg from 37 to 76 years of age(mean 51 year0old). The duration of LCA ranged from 6 months to 30 years. In eleven cases, the lesion was located on the face and in nine cases, on the extremities. Eight cases showed a solitary lesion and twelve cases showed multiple lesions. Transformation to malignancy was not found. 2. Histopathologic study showed sharply circumscribed epidemal neoplasms characterized by large(approximately twice the normal size) uniform keratinocytes with proportinally large nuclei and an increase of melanin in the basal layer. In many cases, hypergranulosis(18 cases) and hyperkeratosis(17 cases) were observed. Occasionally, acanthosis(10 cases), papillomatosis(9 cases) and epidermal atrophy(7 cases) were also observed. However, keratinocyte necrosis was not observed. Moderate solar elastosis and moderate lymphocytic infiltration was observed in the upper part of the dermis in all cases. Mild and moderate melanin incontinence was observed in 14 cases and 1 case respectively. Appendage involvement(follicular infundibulum and acrosyringium) was observed in all cases. 3. The Ki-67 expression of LCA tissue(4.87±3.10%) was significantly higher than the adjacent Ki-67 strongly suggests that LCA is a distinctive entity. (Korean J Dermatol 1999;37(9) : 1261∼1268)

동창의 임상 및 병리조직학적 소견과 Ki-67 발현 양상

서기석(Ki Seuk Suh),김상태(Sang Tae Kim),이기욱(Ki Wook Lee) 대한피부과학회 1999 대한피부과학회지 Vol.37 No.9

N/A Background : Pernio or chilblain is an abnormal reaction to damp cold air, resulting in pruritic erythematous swellings on acral skin. The histologic features are subepidermal edema, diffuse fluffy edema of endothelial cell, and superficial or superficial and deep perivascular lymphocytic infiltrates. Many studies with a large sample size have been conducted in western countries, but there were no collective studies of pernio that have been reported in Korea. Objective : The purpose of this study was to clarify the clinical and histopathologic features of pernio in Korea. Methods : We reviewed the clinical and histopathologic findings in ten patients with pernio who visited the Department of Dermatology, Kosin Medical College Gaspel Hospital during the 10 year period from 1988 to 1997. Results : The results were as follows. 1. The age distribution was in the range of 6∼50 year old. The prevalance was heighest in the third and fourth decade. Sex ratio was 1 : 4 (2 males : 8 females). The duration of symptoms ranged from 4 days to 30 years. All patients developed skin lesions on the acral area. Two male patients developed skin lesions on the hand associated with cold exposure and outdoor works. All patient had itching and burning papules and/or plaques, and one had a painful hard nodule. Laboratory studies were all negative and within normal ranges. 2. Histopathologic features revealed subepidermal edema, diffuse fluffy edema of endothelial cell, and superficial or superficial and deep perivascular lymphocytic infiltrates in all specimens. 3. Treatment includes prophylatic measures against cold and nifedipine(calcium channel inhibiting drug). In more severe cases, pentoxifylline might be helpful. Conclusion : Our study suggests that pernio commonly occurs in twenty to forty year old females during fall and winter. Upon exposure to cold temperatures, the acral skin develops pruritic erythematous papules and plaques. If these lesions persisit for a long time or recur, they may progress to vesicle and ulceration and possibly develop vasculitis which does not respond to therapy well. Thus, a thorough understanding of clinical and histopathologic features of pernio is vital in making a diagnosis, a treatment plan and determining the prognosis. (Korean J Dermatol 1999;37(9) : 1254∼1260)

음식물찌꺼기 퇴비로부터 호염성 미생물의 분리 및 동정

최충식,이오석,권기석 7개 국립대학교 환경연구 논문집 공동발행 위원회 2003 공업기술연구 Vol.3 No.-

An halophilic bacterium was isolated from food-waste. The isolated strain was Gram-negative, aerobic bacterium. The range of temperature for growth of the isolate were 30-40℃. NaCl concentration for growth of it was 5-15%. The isolate was catalase and oxidase positive and sensitive to bacterium. It showed starch hydrolyzing and acid forming characteristics. The morphological, physiological and biochemical characteristics of the isolate resembled those of the Halococcus sp., therefore it was identified as Haloarcula sp. CH-667.

酢酸菌의 遊離, 固定化 菌體를 이용한 豆乳중의 n-hexanal의 脫臭

류병호,김동석,배기철 경성대학교 1989 論文集 Vol.10 No.1

These study was designed to deordorize the off flavor of n-hexanal in defatted soybean milk using the free cells or immobilized cells of acetic acid bacteria, such as Acetobacter aceti, IFO 3281, Acetobacter pasturianus, IFO 13751, and Acetobacter aceti, IFO 13752. When a small amount of acetic acid bacteria was added in defatted soybean milk and incubated at 30℃ for 30min. Deordorization of n-hexanal showed significantly decrease by three kinds of acetic acid bacteria. The optimal conditions of free and immobilized cells for deordorization of n-hexanal was at 50℃-60℃ and, pH 6, and there was no significance difference between free cells and immobilized cells for the deordorization of n-hexanal. Three kinds of acetic acid bacteria was not inhibited activites until 3% sodium chloride and 4.0% sucrose under the same sanie conditions. The immobilized cells system was indicated to maintain the deordorization of n-hexanal for 180 hr.

효소에 의한 꿩고기 가수분해물의 제조

정재홍,김기준,이규희,이석건,오만진 충남대학교 농업과학연구소 1998 농업과학연구 Vol.25 No.1

This studies was carried out to investigate the processing possibility of pheasant meat extracts treated with proteases. The crude protein, aminonitrogen, degree of hydrolysis, yield and amino acid composition of pheasant meat extracts when it was treated with proteases at various temperature and reaction time were analyzed. The crude protein contents of pheasant meat extracts processed in 130℃ were more than when it was done in 100℃, but the contents of aminonitrogen were not quite different between two processing temperature. The content of crude protein and aminonitrogen when pheasant meat was hydrolyzed with protease NP and prozyme A. The yields of pheasant meat extracts, when pheasant meat were treated at 100℃ and 130℃, were from 2.24 to 7.10% and from 5.51 to 10.45%, respectively. And the yield of extraction depended on extraction temperature, kinds of enzyme, amount of enzyme, extraction time. The content of aminonitrogen in pheasant meat extracts treated with enzyme was much higher than any other treatments. And it depended on amount of enzyme, extraction time and temperature. The amount of the amino acids in pheasant meat extracts treated by protease NP were eminently higher than by heat at 100℃ or 130℃.

재두루미에서 Parathion 중독증 발생

권용국,윤선종,김기석 한국수의공중보건학회 2003 예방수의학회지 Vol.27 No.2

The toxicity of organophosphate is due to the disruption of the nervous system of birds through the inhibition of cholinesterase enzymes. Two dead white-napped cranes (Grus vipio) was found at Cholwon which is the most popular wintering site in Korea, and submitted to National Veterinary Research and Quarantine Service for diagnosis. Gross examinations revealed no evidence of any specific disease condition. Histopatholgically, only one bird incidentally had multifocal necrotizing hepatitis with intranuclear inclusion bodies in the liver which was considered to be Inclusion Body Disease of cranes. The contents of gastrointestinal tracts of the birds contained residues of parathion ranged as 67.4 and 42.1 ppm respectively, using by gas chromatography and mass spectrometry. Based on these evidences, parathion was responsible for the cranes mortality.

대구지역 야생조류로부터 분리한 Salmonella속 균의 생물화학적 특성과 Plasmid Profile

윤가리,이영주,김기석,탁연빈 한국수의공중보건학회 2003 예방수의학회지 Vol.27 No.2

The present study was conducted to investigate the prevalence, biochemical properties and plasmid profiles of Salmonella organisms isolated from wild birds in Daegu area during the period from May, 1999 to August, 2000. Twenty strains of Salmonella were identified from 192 samples of heron feces and 86 samples of pigeon feces. Among Salmonella isolates, S. montevideo were the most frequent serotype (16 strains), 3 strains of them were S. typhimurium and the other one was untypable. The biochemical properties of 20 strains of Salmonella isolated were in accordance with the traditional characteristics of Salmonella spp. in the majority of biochemical test such as IMViC, carbohydrate fermentation and amino acid decarboxylation. The majority of isolates were highly susceptible to amikacin, ampicillin, amoxicillin, carbenicillin, cephalothin, ciprofloxacine, chloramphenicol, enrofloxacin, furazolidone, gentamicin, kanamycin, nalidixic acid, polymixin B and sulfamethoxazole, while they were moderately susceptible to streptomycin and tetracyclin. However, the majority of them were highly resistant to nitrofurantoin. In plasmid profile, 21.1 kilobase (Kb), 20 Kb and 13.2 Kb fractions were common to three S. tvphimurium and one untypable strains, and no fraction was shown in all strains of S. montevideo.

Inhibition of protein kinase CKII activity by euchrestaflavanone B purified from Cudrania tricuspidata.

Kim, Soon-Hee,Yoon, Soo-Hyun,Lee, Byong Won,Park, Ki Hun,Kim, Young Ho,Bae, Young-Seuk Pergamon Press 2005 Oncology Research Vol.15 No.6

<P>The CKII (EC 2.7.1.37) inhibitory compound was purified from the root barks of Cudrania tricuspidata and identified as (2S)-2-[2,4-dihydroxy-5-(3-methyl-but-2-enyl)-phenyl]-5,7-dihyroxy-6-(3-methyl-but-2-enyl)chroman-4-one (euchrestaflavanone B). Euchrestaflavanone B was shown to inhibit the phosphotransferase activity of CKII with IC50 of about 78 microM. Steady-state studies revealed that euchrestaflavanone B acted as a competitive inhibitor with respect to the substrate ATP. A value of 16.4 microM was obtained for the apparent Ki. Concentration of 0.8 microM euchrestaflavanone B caused 50% growth inhibition of human cancer cells U937 and HeLa. Euchrestaflavanone B-induced cell death was characterized with the cleavage of poly(ADP-ribose) polymerase and procaspase-3, indicating that the inhibitor triggered apoptosis. Because protein kinase CKII is involved in cell proliferation and oncogenesis, these results suggest that euchrestaflavanone B may function by inhibiting oncogenic disease, at least in part, through the inhibition of CKII activity.</P>

病鷄由來 사모레라속균의 各種 抗菌性 藥劑에 대한 感受性

Ki SeuK Kim(金基錫),Sun Namgoong(南宮琁),In Pil Mo(毛仁筆),Keun Sik Park(朴根植) 한국예방수의학회 1984 예방수의학회지 Vol.8 No.1

The antimicrobial drug susceptibility of 65 isolates of Salmonella recovered from chicken submitted for diagnosis at the Poultry Disease Diagnostic Service Laboratory during 1980 to 1981 was determined by the disc diffusion method. All isolates of Salmonella tested were susceptible to ampicillin, colistin, chloramphenicol, gentamicin, kanamycin and trimethoprim-sulfamethoxazole. A higher percentages of them were resistant to streptomycin(70.8%), sulfonamides(53.9%) and tetracycline(41.5%) while only 4.6% was resistant to nitrofurantoin. Among 65 isolated tested, 61.5% were resistant to 2 or more drugs and a total of 9 resistance patterns were observed. The most frequent resistance pattern of them was Sm Su pattern and foll-lowed by Sm Tc pattern and Sm pattern; these resistance patterns comprised more then half(57.0%) of the total isolates.