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인간포배기 배아의 효과적인 유리화 동결법의 개발을 위한 연구
이상민,이주희,이상원,이승민,윤산현,임진호,박흠대,이성구,Lee, Sang-Min,Lee, Ju-Hee,Lee, Sang-Won,Lee, Seoung-Min,Yoon, San-Hyun,Lim, Jin-Ho,Park, Huem-Dai,Lee, Seong-Goo 대한생식의학회 2003 Clinical and Experimental Reproductive Medicine Vol.30 No.3
Objective: The purpose of this study was to evaluate the survival rate of vitrified blastocyst according to the freezing vessels, equilibration time in cryoprotectant and artificial dehydration method. Methods: Human blastocysts were vitrified after loading onto the plastic straw, open-pulled straw (OPS), electron microscopy grid (EM grid) for 1.5 min or 3 min. They also were directly plunged into LN2 within 30sec. For artificial shrinkage of blastocysts, 36 gauge fine needle was pushed at the cellular junction of the trophectoderm into the blstocoele cavity until it shrank without damage of inner cell mass. Results: The survival rate of vitrified blastocysts on plastic straw, OPS, EM grid as freezing vessels were 26.7, 13.0 and 60.5%, respectively. The survival rate of EM grid was significantly higher than that of plastic straw and OPS (p<0.05). For 1.5 min equilibrium, the survival rates of early blastocyst (EB), middle blastocyst (MB) and late blastocyst (LB) were 64.4, 81.0, and 20.0% respectively. For 3 min equilibrium, the survival rates of EB, MB, and LB were 69.9, 50.0 and 57.5% respectively. The survival rates of EB and MB were significantly higher than that of LB in 1.5 min equilibrium group (p<0.05), however, the significance was not observed in 3 min equilibrium groups. In cytoplasmic shrinkage before vitrification, the survival rates of EB, MB and LB were 92.9, 100 and 75.9% respectively. The survival rate of MB was significantly higher than that of LB (p<0.05). The survival rates of vitrified blastocysts by artificial dehydration and slow-frozen blastocysts were not significantly different as 88.9 and 66.7%, respectively. Conclusion: This study showed that the vitrification of human blastocysts using EM grid and artificial dehydration is an effective method. Therefore, these methods would be an useful techniques for blastocyst cryopreservation.
민헌식,이성환 한국공작기계학회 2002 한국생산제조학회지 Vol.11 No.3
Though the laser cutting process is increasingly used in industry, a process automation and systematic database is still yet to be developed. In this study, as the fundamental step toward the construction of a reliable process expert system, a laser cutting quality monitoring/analysis system is developed based on simulation and experimental results. The relations between laser process parameters and laser cutting surface quality parameters such as kerf geometry, striation, surface roughness and dross formation are characterized and analyzed. A graphical user interface is used to visualize the results.
河床變動 豫測에 관한 硏究 : 密陽江이 合流하는 洛東江 本流地域을 中心으로
閔丙亨,李承輝,閔一圭,兪甲鎔,吳尙龍 동아대학교 건설기술연구소 1996 硏究報告 Vol.20 No.1
In this study, when we collect river bed sediment at the point where mainstream of Nak Dong river join branch of Mil Yang river, the change of river bed around downstream area was estimated and based on experimental data using three dimensional unsteady flow model about the water level to quantity of water and velocity and river bed change through numerical imitation experiment and hydraulic model experiment.
민병형,김가현,이승휘,이정환,유상호 동아대학교 건설기술연구소 2001 硏究報告 Vol.25 No.1
The concern for a cleaner environment together with the need to conserve and reuse our resources has created a challenge for which sanitary engineers, working with environmentalist, will be called upon to find new solutions over the next few years. The result of the study of development of the River's waterfront run as follows. 1. The introduction of the water facilities (which is) utilized in the neighborhood by adjacent citizens and the development of the water site (which is) equipped with facilities for environment must be actually progressed. 2. The concerned authority's favorable complation of the budget must be made up to construct the facilities as the district. 3. Close investigation on the coast and arbores and so on, must be progressed to develop the river in a manner (which is) close to nature.
海雲臺 海濱 侵蝕防止에 관한 實驗的 硏究 : 突堤 및 離岸堤의 組合構造物에 대하여
閔丙亨,李承輝,민일규 東亞大學校 海洋資源硏究所 1988 硏究論文集 Vol.1 No.1
Recently the HAEUNDAE beach has an impact on intense erosion phemomena. At this point, the purpose of this study is to examine the sedimentation effect by using a three-dimensional hydraulic model test when a jetty is set up. jetty From the results of the experiment it can be concluded that the optimum length of jetty would be L₁=300m, direction angle would be θ=22.5°when, H=3.0m and T=8.0 sec. A jetty and offshore breakwater are also considered as a compound structure. From this experiment the optimum length of offshore breakwater would be L₂=200m. A jetty and T-Type jetty are also comtemplated as compound structure. From this experiment the optimum length of T-Type jetty would be L₃=300m.
민헌식,이성환,안선응 한국공작기계학회 2002 한국생산제조학회지 Vol.11 No.3
As laser cutting process is widely used in industry, an automated on-line process control system has become very important. In this paper, development of a laser cutting monitoring system, which is regarded as the fundamental step toward automation of the process, is presented. Acoustic emission and an artificial neural network were used for the monitoring system. With given process parameters including laser power and scanning speed, the system can predict the suitability of laser beam for the cutting of a stainless steel (STS304) plate.
민승기 大韓顎顔面成形再建外科學會 1995 Maxillofacial Plastic Reconstructive Surgery Vol.17 No.2
Vestibuloplasty are following categories : Mucosal advancement(submucous), secondary epithlization(reepithelization) and grafting vestibuloplasty. Although certain procedures are indicated for alveolar bone loss and sulcus shortening, relapse can occur. Every efforts to minimize or compensate for it is controversy. O'Steen(1970) reported the mucous graft methods that none of vestibular shrinkage and graft contracture. 15patients in mucous graft vestibuloplasty with fibrin adhesive system(Beriplast) were taken in cases of alveolar bone resorption and mucosal shortening due to traumatized alveolar bone defects, senile atrophic alveolar bone, postoperative cyst or tumor resection, edentulous alveolar bone loss, and others. A technique in the use of small piece of palatal mucosa(1x 20mm) from the lateral aspect of the palate with adhesive system provided to secure the skin grafts, avoid stent fixation, postoperative patient's comfort and less time-consuming than the standard technique, especially excellent bleeding control.
금형면 자동 다듬질 전문가 시스템 개발에 관한 연구-Ⅱ : 통합 연마 파라미터를 사용한 최적 가공 구현 및 전문가 시스템 구축
민헌식,이성환,안유민,조남규,한창수 한국공작기계학회 2002 한국생산제조학회지 Vol.11 No.1
To reduce the cost and increase reliability of die and mold products, automation of the finishing (polishing) process is essential. A major element of automation is a reliable database and a knowledge base for polishing status. This paper presents a polishing expert system which can determine optimal polishing sequences and conditions by using an empirical formula and an experimental database. The simplex method was used for the cure fittings of the experimental results. Also a graphical user interface, which visualizes the optimized results, was developed.
금형면 자동 다듬질 전문가 시스템 개발에 관한 연구-Ⅰ : DB 구축을 위한 회전 및 진동 연마 가공의 실험적 연구
민헌식,이성환,안유민,조남규,한창수 한국공작기계학회 2002 한국생산제조학회지 Vol.11 No.1
In manufacturing die and mold, polishing is important as it takes as mush as 50% of the production cost. In this research, an attachable type polishing device to a CNC machining center was developed. Experiments were done with a specially designed rotation type polishing device. Also an ultra-sonic (Vibration type) waving device was introduced to acquire finer surface finishes. From the constructed data base based on the experimental results, it is shown that optimal polishing conditions are generated by the combined use of the rotation type tool and the vibration type tool.