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Genetics of Bacillus thuringiensis and its application
임성렬 한국생화학분자생물학회 1990 생화학분자생물학회 소식 Vol.10 No.2
In this article, genetics of BaciUus thuringimwis (BT) strains, which produce insect specific delta-endotoxin, and industrial application are generally reviewed. Particularly, molecular biological characters of coleopteran specific delta-endotoxin, which is produced from B. turingiemis subsp. tenberimis (BTT), are discussed including a transformed tobacco plant with the gene of toxin. In addition, a possible method of the maintenance for the stability of the BT-toxin in field is proposed for the effective control of insect pest.
착색사출성형품에서 안료 및 성형조건이 수축에 미치는 영향
정영득,한성렬 釜慶大學校 1999 釜慶大學校 論文集 Vol.4 No.1
The pigments used for coloring of plastic parts influence considerably on the parts quality such as precision, appearance and strength. In this research, we explored the effects of injection molding condition and pigment's material properties on the shrinkage in the injection molded PP(polypropylene) parts. Injection molding experiments were conducted to collect data that were used to relations between parts qualities and injection condition such as melt temperature, injection pressure and holding pressure with various pigment. The results for injection conditions show that shrinkage was increased according to increasing melt temperature and decreased according to increasing injection pressure and holding pressure. And the results for pigments show that shrinkage in case of adding organic pigments was shown larger that inorganic pigments and red among all pigments were shown largest shrinkage in all case.
( Seong Lyul Rhim ) 한국응용생명화학회 1998 Applied Biological Chemistry (Appl Biol Chem) Vol.41 No.2
The transgenic tomato plants showing the insecticidal activity against the coleopteran insect larvae have been bred to the 4th generation (R₄). The Bacillus thuringiensis subsp. tenebrionis (B.t.t.)-toxin gene and the expression were detected in the R₄ transgenic plants. The expression of the toxin gene conferred a coleopteran insect larvae tolerance to the transgenic tomato plants. The ploidy levels of the R₄ transgenic plants were diploid. The results indicated that the toxin gene was inherrited to the next generation and expressed. Such a molecular breeding can provide a method for a permanent control of insects a agronomic relevance.
Rhim, Seong Lyul,Kim, Il Gi,Jin, Tae Eun,Lee, Jin Hyoung,Kuo, Ching I,Suh, Suk Chul,Huang, Li Chun The Korean Society of Plant Biotechnology 2004 Plant molecular biology and biotechnology research Vol.6 No.1
A modified $\delta$-endotoxin gene of Bacillus thuringiensis ssp. tenebrionis (B.t.t.), encoding a coleoptera-specific toxin, was utilized to transform citrus plants, Citrus reticulata Blanco 'Ponkan' mandarian. By co-culturing the nucelli with Agrobacterium tumefaciens harboring the modified gene in the binary vector pBinAR-Btt, the chimeric toxin gene was transferred into citrus plants. The transgenic plants were selected on modified Murashige and Skoog medium containing kanamycin. Hybridization experiments demonstrated that the transgenic plants contained and expressed the toxin protein gene.