국내서식 살모사 사독의 면역학적 특성분석 (2)

성원근,정경태,김현희,박재구,박영미아,오희복 국립보건연구원 1998 국립보건원보 Vol.35 No.-

자궁내막암 환자의 재발여부 확인에 있어 FDG-PET scan 의 유용성에 대한 고찰

홍성진 ( Hong Seong Jin ),이삼미 ( Lee Sam Mi ),박천숙 ( Park Cheon Sug ),김호아 ( Kim Ho A ),김법종 ( Kim Beob Jong ),김문홍 ( Kim Mun Hong ),최석철 ( Choe Seog Cheol ),유상영 ( Yu Sang Yeong ),이경희 ( Lee Gyeong Hui ) 대한산부인과학회 2004 Obstetrics & Gynecology Science Vol.47 No.2

목적 : Inhibin은 α-subunit와 β-subunit로 구성된 이질 이량체의 당단백으로 β-subunit의 차이에 의해 inhibin A (α-βA)와 inhibin B (α-βA)로 구분된다. 여성에서 inhibin은 주로 난소의 과립막세포 및 황체에서 생산되어 뇌하수체의 FSH 분비를 억제한다고 알려져 왔으나, 임신중 태반과 남성의 고환에서도 생산된다는 사실이 밝혀져 다양한 생리적 작용이 추정되고 있으며 최근에 inhibin A와 B를 분리 측정할 수 있는 방법이 개발되어 inhibin에 대한 연구가 활발히 진행되고 있다. 이에 저자들은 정상 월경주기를 가진 가임기 여성, 폐경이행기 여성, 폐경후기 여성에서 inhibin A와 inhibin B의 농도를 측정하여 월경주기에 따른 정상치의 변화와 노화에 따른 농도 변화를 관찰하고, 난소에서 면역조직화학적 염색법으로 inhibin A와 inhibin B의 발현 양상과 변화를 비교 관찰하여 inhibin의 정확한 생리작용, 폐경이행기에서 inhibin의 역할 및 폐경이행기와 폐경의 예측 지표의 사용 가능성을 알아보고자 하였다. 연구 방법 : 월경주기와 난소기능이 정상이면서 난소종양이 없는 정상 가임기 여성 160명, 폐경이행기 여성 60명, 폐경후기 여성 20명에서 정맥혈을 채혈하여 혈중 inhibin A와 inhibin B 농도를 ELISA로 측정하였고, 그중 정상 가임기 여성 35명, 폐경이행기 여성 20명, 폐경기 여성 5명의 난소 조직을 inhibin α, βA 및 βB subunit에 대한 단클론 항체를 이용하여 면역조직화학적 염식을 시행하였다. 결과 : 1. 정상 가임기 여성에서 혈중 inhibin A 농도는 중증식기까지 낮은 농도를 유지하다 후증식기 (16.53±1.57 pg/ml)부터 증가하기 시작하여, 중분비기 (45.85±2.08 pg/ml)에 최고치에 이른 후 서서히 감소하는 경향을 보였다. Inhibin B 농도는 전증식기 (65.40±4.08 pg/ml)부터 증가하기 시작하여 증식기 동안 계속 높은 농도를 유지하다가 배란기 (110.74±9.83 pg/ml)에 최고치에 이른 다음 분비기에 급격히 감소하였다. 2. 폐경이행기 여성에서 혈중 inhibin A 농도는 증식기에 6.68±0.53 pg/ml, 분비기에 21.78±3.61 pg/ml로 모두 정상 가임기 여성과 비교하여 유의하게 낮았다 (P<0.01). Inhibin B의 농도는 증식기에 52.16±7.46 pg/ml, 분비기에 22.41±6.73 pg/ml로 모두 정상 가임기 여성과 비교하여 유의하게 낮았다 (각각 P<0.01, P=0.025). 3. 폐경후기 여성에서 inhibin A와 inhibin B 농도는 모두 검출되어 않았다. 4. 정상 가임기 여성에서 inhibin α subunit의 면역염색반응은 과립막세포, 난포막세포 및 황체에서 강하게 발현되었다. Inhibin βA의 면역염색반응은 난포에서는 거의 발현되지 않았으나 황체에서는 관찰되었다. Inhibin βB의 면역염색반응은 일차난포에서 처음 발현되어 성장난포와 성숙난포의 과립막세포와 난포막세포에서 관찰되었으나 Inhibin α보다는 약했고 황체에서는 거의 관찰되지 않았다. 5. 폐경이행기 여성에서 inhibin subunit들의 면역염색반응은, 정상 가임기 여성과 같은 양상으로 발현되었으나, 발현정도는 약했다. 모든 면역염색반응은 과립막세포보다 난포막세포에서 더 강하게 나타났다. 6. 폐경후기 여성에서 inhibin subunit들의 면역염색반응은 전혀 관찰되지 않았다. 결론 : Inhibin A는 후증식기 성숙난포에서 처음 생산되기 시작하여 배란 후 황체에서 주로 생산되며, Inhibin B는 일차난포에서 생성되기 시작하여 배란 시까지 분비되므로 inhibin A는 황체기능을, inhibin B는 난포의 기능을 반영한다. 폐경이행기가 되면 inhibin A와 B의 분비는 급속히 감소하게 되어 폐경 후에는 난소에서 생성되지 않는다. 따라서 inhibin A와 B는 여성의 월경주기에 따라 생산과 분비가 서로 다른 호르몬으로 난자 성숙과 난포발달에 관여하며, 난소의 노화 정도를 측정하는 폐경이행기의 지표로 이용될 수 있을 것이다. Objective : To understand the physiologic effects and secretion pattern of inhibin A and inhibin B during menstrual cycle and menopausal transition, inhibin A and inhibin B levels were measured. And to detect any changes in expression of inhibins in human ovary with age, we examined immunohistochemical staining of α, βA, and βB subunits of inhibin in ovarian tissues. This study was also designed to investigate whether or not inhibin is an early marker for menopausal transition. Methods : Inhibin A and inhibin B levels were measured in 320 samples from normal reproductive women, in 60 from perimenopausal women, and in 20 from menopausal women by ELISA. And we examined the immunohistochemical staining of α, βA, and βB subunits of inhibin in ovarian tissues of 35 normal reproductive, 20 perimenopausal, and 5 menopausal women, respectively. Results : In the normal reproductive women, inhibin A begins to increase in the late proliferative phase (16.53±1.57 pg/ml), reaches the peak in the mid-secretory phase (45.85±2.08 pg/ml), reaches the peak in the ovulatory phase (110.74±9.83 pg/ml), reaches the peak in the ovulatory phase (110.74±9.83 pg/ml), and thereafter declines rapidly. In the perimenopausal women, mean inhibin A serum concentration was 6.68±0.53 pg/ml during proliferative phase and 21.78±3.61 pg/ml during secretory phase, which were significantly lower than that of the same phase in the normal reproductive women (P<0.01). Mean inhibin B serum concentration was 52.16±7.46 pg/ml during proliferative phase and 22.41±6.73 pg/ml during secretory phase, which were significantly lower than that of the same phase in the normal reproductive women (P<0.01, P=0.025). In the menopausal women, both inhibin A and inhibin B were not detected. In the normal reproductive women, we observed strong immunostaining for α subunit in granulosa cells, theca cells, and corpus luteum. Immunostaining for βA subunit was observed in corpus luteum, but not in growing follicles. Immunostaining for βB subunit was observed in primary follicle, granulosa and theca cells of growing follicle, and mature follicle, but less strong than immunostaining for α subunit. No staining for βB subunit was observed in the corpus luteum. In the perimenopausal women, immunostaining for inhibin subunits were observed in the same pattern as that of the normal reproductive women, but weaker. Stronger immunostaining was observed in theca cells than granulosa cells. In the menopausal women, none of the immunostaining of inhibin subunits were observed.

한국인 좌심실 비대증 환자들에서 파브리병 선별검사의 의의

박형두,조성윤,이수연,전은석,박승우,이상훈,이상철,최진오,박성지,장성아,김형관,기창석,김종원,진동규,Park, Hyeong-Du,Jo, Seong-Yun,Lee, Su-Yeon,Jeon, Eun-Seok,Park, Seung-U,Lee, Sang-Hun,Lee, Sang-Cheol,Choe, Jin-O,Park, Seong-Ji,Jang, Seong-A,K 대한유전성대사질환학회 2014 대한유전성대사질환학회지 Vol.14 No.2

목적: 파브리병(Fabry disease)은 alpha-galactosidase A의 결핍으로 인하여 리소좀에 globotriaosylceramide(Gb3)가 축적되어 여러 장기에 이상을 일으키는 질병이다. 본 연구에서는 파브리병의 만성 합병증 중 심장 질환을 주로 보이는 환자들, 그 중에서도 좌심실 비대증을 보이는 한국인 환자들을 대상으로 파브리병의 빈도를 알아보고자 하였다. 방법: 좌심실비대증을 진단받은 환자 257명을 연구대상으로 선정하였고, 남성이 172명(평균 56세, 범위 30-81세), 여성이 84명(평균 66세, 범위 45-85세)이었다. 파브리병 선별을 위하여 고성능액체크로마토그래피-탠덤질량분석기를 이용하여 소변 Gb3 농도를 측정하였다. 확진은 형광분석법에 의한 말초혈액의 alpha-galactosidase A 활성도와 염기서열분석법에 의한 GLA 유전자 돌연변이 유무를 검사하여 이루어졌다. 결과: 소변 Gb3 검사에서 cutoff (25 ug/mmoL creatinine)를 초과하는 환자는 4명이었지만, 최종적으로 추가 검사를 통해 진단된 파브리병 환자는 여성 환자 한 명이었다(1/257명, 0.4%). 확진된 환자는 54.3 ug/mmoL creatinine의 Gb3 농도와 15.5 nmole/hr/mg protein (참고범위, $55.2{\pm}12.7nmole/hr/mg$ protein)의 alpha-galactosidase A 활성도를 보였다. GLA 유전자에서는 c.796G>A (p.D266N) 돌연변이가 이형접합체로 관찰되었다. 추가로 시행한 가족검사에서 환자의 딸은 아직 파브리병의 증상을 보이지 않았지만, 엄마와 같은 GLA 돌연변이(c.796G>A)를 가지고 있었으며, alpha-galactosidaseA 활성도는 42.5 nmole/hr/mg protein, 소변 Gb3 농도는 25.5 ug/mmoL creatinine을 나타냈다. 결론: 한국인 좌심실 비대증을 가진 환자들에서 파브리병의 유병율은 0.4%였다. 유병율이 낮아 보임에도 불구하고, 파브리병 진단 전 환자와 가족 구성원을 발견할 수 있는 장점 덕분에 선별검사의 의의가 있는 것으로 사료된다. Objectives: Fabry disease (FD) is a lysosomal storage disease caused by the inappropriate accumulation of globotriaosylceramide (Gb3) in tissues due to a deficiency in the enzyme ${\alpha}$-galactosidase A. Hypertrophic cardiomyopathy is one of the chronic complications of FD. We tried to evaluate the prevalence of Fabry disease in the Korean patients with left ventricular hypertrophy (LVH). Methods: A total of 257 patients with LVH were recruited and they were 172 males (mean 56 years, range 30-81 years) and 84 females (mean 66 years, range 45-85 years). Urinary Gb3 was used to screen FD by high performance liquid chromatography-tandem mass spectrometry. Confirmatory tests were done by alpha-galactosidaseA activity using fluorometric assay and by GLA mutation analysis using sequencing. Results: Four patients were screening positive by urinary Gb3 analysis (cutoff, 25 ug/mmol creatinine). But, one female patient was diagnosed with FD confirmed by enzyme analysis in leukocytes as well as by genetic analysis (1/257 patients, 0.4%). She showed 54.3 ug/mmoL creatinine of Gb3 and 15.5 nmole/hr/mg protein (reference range, $55.2{\pm}12.7nmole/hr/mg$ protein) of alphagalactosidase A activity. And she had a heterozygous GLA mutation of c.796G>A (p.D266N). Her daughter was found to be a carrier for FD confirmed by GLA mutation analysis. Asymptomatic carrier showed 25.5ug/mmol creatinine of Gb3 and 42.5 nmole/hr/mg protein (reference range, $55.2{\pm}12.7nmole/hr/mg$ protein) of alpha-galactosidase A activity. Conclusions: The prevalence of FD in Koran patients with LVH was detected as 0.4%. Although the prevalence seems to be low, screening studies are of great importance for detecting hidden cases as well as for identifying other effected family members.

Investigation of test performance of the dual reminder A-Not A (DR A-Not A) in comparison to 3-AFC for discriminating samples of drinking water

Mun, Ji-Won,Kim, Min-A,Sim, Hye-Min,Lee, Hye-Seong Elsevier 2019 Food quality and preference Vol.77 No.-

<P><B>Abstract</B></P> <P>Although the relative performance of sensory discrimination methods is well theorized in Thurstonian modeling and signal detection theory (SDT), empirical research is needed to investigate how such theorized models could be validated in complex sensory testing situations of food and beverages. This paper presents a practical procedure to utilize the existing SDT-based A-Not A sensory discrimination model based on a beta-strategy for detecting the off-sensory quality of samples of drinking water and validates the model for effective and efficient sensory quality management of food and beverages. Dual reminder A-Not A (DR A-Not A) using two tastings of the reference stimulus before evaluating every test stimulus is proposed as the optimal test procedure because it uses more sensitive test sequences and facilitates subjects’ familiarization with the standard quality of the reference. To test the performance of DR A-Not A, 3-AFC, which also uses three stimuli and is the recommended method for detecting odor or taste stimuli by ASTM, was used as a control method. In Experiment 1, 98 subjects each performed both DR A-Not A and 3-AFC. Based on these results, only sensitive subjects were selected for the next experiment, in which they were divided into two equally well performing groups. In Experiment 2, each group performed either DR A-Not A or 3-AFC over three repeated sessions. The results confirmed that the A-Not A beta-strategy was adopted for DR A-Not A and that its test performance was improved over replications. These results suggest that although DR A-Not A is an unspecified difference test method and does not use a forced-choice task, embedding the familiarization procedure for the reference renders it an effective sensory difference method for the sensory quality management of drinking water.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Dual reminder A-Not A(DR A-Not A) designed to detect off-sensory characteristics. </LI> <LI> This method was tested on practicality of quality management of drinking water. </LI> <LI> For using DR A-Not A with two odd stimuli, the optimal β-strategy was adopted. </LI> <LI> Performance of unspecified DR A-Not A was comparable to that of specified 3-AFC. </LI> <LI> Dual reminder scheme found to be an efficient practice to utilize SDT A-Not A model. </LI> </UL> </P>

청가시덩굴 추출물의 기능성 원료 표준화를 위한 지표성분 Resveratrol, trans-Scirpusin A의 분석법 개발 및 검증

권진관(Jin Gwan Kwon),정연우(Yeon Woo Jung),최윤혁(Yun-Hyeok Choi),이지은(Ji Eun Lee),정원식(Wonsik Jeong),이정아(Jung A Lee),최춘환(Chun Whan Choi),안은경(Eun-Kyung Ahn),최용문(Yongmun Choi),홍성수(Seong Su Hong) 한국식품영양과학회 2022 한국식품영양과학회지 Vol.51 No.11

본 연구는 HPLC를 이용하여 청가시덩굴 추출물을 개별인정형 건강기능식품의 기능성 원료로 개발하기 위한 원료 표준화의 일환으로, 청가시덩굴 추출물의 지표성분을 resveratrol과 trans-scirpusin A로 설정하고 이에 대한 HPLC 분석법을 확립하여 유효성의 검증을 실시하였다. 분석법 유효성 검증은 특이성, 직선성, 정확도, 정밀도, 검출한계 및 정량한계 등을 통해 분석법의 신뢰성을 검증하였으며, 그 결과 표준용액과 청가시덩굴 추출물 간의 HPLC 크로마토그램 및 UV spectrum의 일치 여부 등의 비교를 통해 다른 물질과 간섭 없이 피크가 분리된 것으로 특이성을 확인하였다. 또한 표준용액 검량선의 상관계수(R²)는 0.9999로 매우 우수한 직선성으로 관찰되어 분석에 적합한 것으로 확인되었으며, 검량선의 기울기 및 표준편차를 이용한 검출한계는 resveratrol이 0.98 μg/mL, trans-scirpusin A는 0.49 μg/mL였고 정량한계는 resveratrol이 2.98 μg/mL, trans-scirpusin A는 1.48 μg/mL로 각각 확인되었다. 청가시덩굴 추출물에 표준물질을 3개 농도 첨가하고 분석한 회수율은 resveratrol이 98.77~99.24%, trans-scirpusin A는 98.45~99.45%로 나타나 정확성이 있는 것을 확인할 수 있었다. 청가시덩굴 추출물의 조제 농도 2.2, 4.4 및 6.6 mg/mL에서 반복성은 resveratrol이 0.99~1.22%, trans-scirpusin A는 1.12~1.32%를, 실험실 내 정밀성에서는 일내 정밀성은 resveratrol이 0.67~0.87%, trans-scirpusin A는 1.18~1.33%로 나타났고 일간 정밀성은 resveratrol이 0.93~1.22%, trans-scirpusin A는 1.33~2.27%로 확인되어 본 분석법은 정밀성이 있음을 확인할 수 있었다. 이상의 분석결과를 통해 확립된 청가시덩굴 추출물의 지표성분인 resveratrol과 trans-scirpusin A의 HPLC 분석법은 적합한 시험법으로 검증되었으며, 본 시험법은 향후 청가시덩굴 추출물의 건강기능식품 기능성 원료 개발과 표준화를 위한 기초자료로 활용될 것으로 사료된다. This study was undertaken to establish an analytical method using high-performance liquid chromatography (HPLC). HPLC for the standard determination of resveratrol and trans-scirpusin A as functional ingredients in Smilax sieboldii extract. We evaluated the specificity, linearity, accuracy, precision, limit of detection (LOD), and limit of quantitation (LOQ) of various analytical methods for detecting resveratrol and trans-scirpusin A using HPLC. The specificity was confirmed by the chromatogram obtained using the HPLC analytical method. Also, the results of UV and the coefficient of correlation (R²) obtained was 0.999, which confirmed that this was a suitable analysis with high linearity. The LOD was 0.98, 0.49 μg/mL, and LOQ was 2.98, 1.48 μg/mL, which was confirmed as a suitable limit level for the analysis of resveratrol and trans-scirpusin A content in the S. sieboldii extract. The recovery of resveratrol and trans-scirpusin A content was determined to be 98.77±0.73∼99.24±1.47% and 98.45±1.18∼99.45±1.66%, respectively, indicating high accuracy. The intra-day repeatability and the intra-laboratory precision of the daily repetition were confirmed to be 0.67∼0.87%, 1.18∼1.33% and 0.93∼1.22%, 1.33∼2.27%, respectively, for trans-scirpusin A, for the relative standard deviation. These results indicate that the reported HPLC method is simple, reliable, and reproducible for the detection of resveratrol and trans-scirpusin A in S. sieboldii extract.

부산지역 유흥업소 종사여성으로부터 분리된 HPV16형의 발암유전자(E6/E7) 돌연변이 유형 분석

민상기(Sang-Kee Min),김성순(Sung Soon Kim),최병선(Byeong-Sun Choi),장대호(Dai-Ho Jang),이미옥(Mee-Ok Lee),최성화(Seung-Hwa Choi),김남호(Nam-Ho Kim),박연경(Yon-Koung Park),정영아(Yeong-A Jeong),김성준(Seong-Joon Kim),빈재훈(Jae-Hun Bi 한국생명과학회 2009 생명과학회지 Vol.19 No.6

HPV-16형의 염기배열 변이는 지역적, 인종적으로 특징적인 차이가 있으며 특히 HPV-16형 E6/E7 유전자의 특정 염기서열변이는 자궁경부암 및 자궁상피내 신생종양물의 발생을 일으키는 고위험 요인으로 알려져 있다. 본 연구는 2007년 부산지역 유흥업소 종사여성으로 분리된 HPV-16형 19건을 대상으로 E6/E7 유전자 영역(nt 34-880)을 표적으로 지역적 염기서열 변이를 조사하였다. nucleotide 수준에서 HPV16형 E6 유전자는 T178G (n=11), T178A (n=1), T350G (n=4), A442C (n=2), A104T, A111G, C116T, G145T, T183G, C335T, G522C 등 11종의 변이주가 발견되었고, E7 유전자는 A647G (n=12), A645C, A777C, G663A, T732C, T760C, A775T, T789C, T795G 등 9종의 변이주가 발견되었다. 아미노산 수준에서는 HPV-16형 E6 단백질의 경우 D25E (n=12), L83V (n=4), E113D (n=2), M1L, Q3R, P5S, Q14H, D25N, I27R, H78Y, C140S 등 11종의 변이주를, HPV16형 E7 단백질의 경우 N29S (n=12), L28F, T72S 등 3종의 변이주를 관찰할 수 있었다. 본 연구 결과, 부산지역의 HPV-16형 E6/E7 우점 돌연변이주는 E6 D25E (75%), E7 N29S (78%)로 각각 나타났다. 앞으로 자궁경부암 환자 및 일반여성을 포함한 더 많은 모집단을 대상으로 HPV-16형 E6/E7의 intratypic variants를 비교 조사하여 실제 HPV-16형 E6/E7 어떤 변이주가 자궁경부암 유발 위험성과의 관련성은 더 많이 연구되어져야 할 것으로 사료된다. Recent studies have reported that the distribution of HPV-16 sequence variation differs geographically, and more specifically that HPV-16 E6/E7 intratypic variants might carry a high risk for development of ICC (invasive cervical cancer) and CIN (cervical intraepithelial neoplasia) in a given population. To investigate the genetic diversities of HPV-16 E6/E7 oncogene by region, we collected nineteen HPV-16 isolates from sexually high-risk women in Busan, and analyzed the HPV-16 E6/E7 coding regions (nt 34 to 880) with HPV-16 E6/E7 specific PCR amplification. At the nucleotide level, eleven variants of the E6 genes and nine variants of the E7 genes were identified as follows: E6 T178G (n=11), E6 T178A (n=1), E6 T350G (n=3), E6 A442C (n=2), E6 A104T, E6 A111G, E6 C116T, E6 G145T, E6 T183G, E6 C335T, E6 G522C and E7 A647G (n=12), E7 A645C, E7 A777C, E7 G663A, E7 T732C, E7 T760C, E7 A775T, E7 T789C and E7 T795G, respectively. At the amino acid level, the isolated HPV-16 E6 and E7 genes showed eleven E6 variants: E6 D25E (n=12), E6 L83V (n=4), E6 E113D (n=2), E6 M1L, E6 Q3R, E6 P5S, E6 Q14H, E6 D25N, E6 I27R, E6 H78Y, E6 C140S and three E7 variants: N29S (n=12), L28F, T72S. HPV16 E6 L83V, the dominant variant in the Caucasian population, showed relatively low frequencies in our study population. We elucidated that the dominant HPV-16 E6/E7 variants were HPV-16 E6 D25E (63.2%) and HPV-16 E7 N29S (63.2%), which were phylogenetically included in Asian lineage. Further study is needed to evaluate the risk of cervical cancer related HPV-16 E6/E7 intratypic variants in the Korean population.

Wogonin, a flavonoid isolated from Scutellaria baicalensis, has anti-viral activities against influenza infection via modulation of AMPK pathways

SEONG, R.-K.,KIM, J.-A.,SHIN, O. S. AEPress, s.r.o. 2018 Acta virologica Vol.62 No.1

<P>Wogonin, a flavonoid isolated from Scutellaria baicalensis, has attracted increasing scientific attention in recent years because of its potent anti-tumor activity. Its role during viral infection has largely been unexplored. Wogonin treatment effectively suppressed both influenza A and B virus replication in Madin-Darby Canine Kidney (MDCK) cells and human lung epithelial (A549) cells. In contrast, wogonin treatment following influenza A virus infection led to up-regulation of interferon (IFN)-induced antiviral signaling. Additionally, influenza A virus infection in A549 cells induced 5' adenosine monophosphate- activated protein kinase (AMPK) phosphorylation and activation in a time-dependent manner and wogonin treatment led to the suppression of AMPK phosphorylation. Furthermore, the treatment with AMPK-specific inhibitor (compound C; CC) attenuated influenza A virus replication. These data suggest that wogonin possesses a potent anti-influenza activity mediated by regulation of AMPK activation, suggesting that wogonin has the potential to be developed as an anti-influenza drug.</P>

소화기 ; 대전 지역에서 급성 A형 간염의 유전자형에 따른 임상 특성 고찰

이영우 ( Young Woo Lee ),양현웅 ( Hyeon Woong Yang ),이진아 ( Jin A Lee ),윤기호 ( Ki Ho Yun ),양성은 ( Seong Eun Yang ),이민지 ( Min Ji Lee ),박세영 ( Se Young Park ),김새희 ( Sae Hee Kim ),이향이 ( Hyang Ie Lee ),이윤정 ( Yun Ju 대한내과학회 2011 대한내과학회지 Vol.80 No.5

목적: B형 간염이나 C형 간염 등은 유전자형에 따른 차이가 알려져 있지만, 국내에서 증가하는 IIIA 유전자형과 기존의 IA 유전자형에 의한 간염의 차이가 거의 알려진 바 없다. 따라서 대전 지역에서 발생한 급성 A형 간염의 유전자형에 따른 차이를 알고자 한다. 방법: 2009년 11월부터 2010년 6월까지 급성 A형 간염으로 진단되면서 유전자형이 확인된 81명의 환자를 대상으로 임상적 소견들과 검사실 소견들을 전향적으로 연구하였다. 결과: 평균 나이는 32.6±7.4세이며, 평균 입원 기간은 7.7±2.4일이었다. 직업은 서비스직, 사무직 그리고 교육직이 가장 많았다. 유전자형 간의 급성 간염에 따른 증상들의 종류는 별다른 차이가 없었다. 유전자형에 따른 증상의 빈도는 IIIA 유전자형에서 구토와 설사가, IA 유전자형에서는 복통과 피부 반점이 상대적으로 많았다. 검사실 소견에서는 IIIA 유전자형에서 헤모글로빈의 평균, CRP 평균, AST 최대 수치 평균은 상대적으로 유의하게 높았다. 그리고 AST와 ALT 및 총 빌리루빈 최대 수치들과 INR의 수치가 IIIA 유전자형에서 상대적으로 높게 분포하는 경향을 보였다. 결론: 대전 지역에서 유행하는 급성 A형 간염에서 IIIA 유전자형에 따른 급성 간염은 기존의 IA 유전자형과 차이가 있을 것으로 보이며, 다기관 연구를 통한 심도 깊은 연구가 필요하겠다. Background/Aims: Acute viral hepatitis A is a major health problem in Korea and the influx of genotype IIIA is thought to be one reason. We examined the differences in the clinical characteristics and laboratory findings of genotypes IA and IIIA in Daejeon. Methods: From November 2009 to June 2010, 81 patients positive for IgM anti-HAV were enrolled prospectively. The hepatitis A was genotyped using real-time polymerase chain reaction. The clinical characteristics and laboratory results were compared on the Results: The mean patient age was 32.6±7.4 years. The mean hospitalization was 7.7±2.4 days. The patient occupation varied. Clinically, vomiting and diarrhea were relatively more prevalent in genotype IIIA than in IA. Abdominal pain and skin spots were relatively more prevalent in genotype IA than in IIIA. The hemoglobin, peak aspartate aminotransferase (AST) level, and C-reactive protein were statistically higher in genotype IIIA than in IA. The distributions of the peak AST, alanine aminotransferase (ALT) and total bilirubin values tended to be perched in genotype IIIA than in IA. The international normalized ratio (INR) tended to be slightly prolonged in genotype IIIA than in IA. Conclusions: Recently, genotype IIIA of acute viral hepatitis A has become prevalent in Daejeon. Hepatitis A genotype IIIA probably causes worse laboratory abnormalities than genotype IA.

Degree of satisfaction-difference (DOSD) method for measuring consumer acceptance: Comparative and absolute measures of satisfaction based on signal detection theory

Kim, Min-A.,van Hout, Danielle,Lee, Hye-Seong Elsevier 2018 Food quality and preference Vol.68 No.-

<P><B>Abstract</B></P> <P>Recently, we have proposed the degree of satisfaction-difference (DOSD) method to measure changes in consumer product acceptance (Kim, van Hout, Dessirier, et al., 2018). The DOSD method utilizes A-Not A with reminder format to improve the stability and validity of the consumer evaluation by reminding consumers of the evaluative criteria with the reference sampling and by controlling the sequence effects on each sampling of the blind test product. Using the data obtained from DOSD method, in this paper, a way to compute a measure of the absolute degree of satisfaction for a product based on signal detection theory (SDT), referred to as <I>d'</I> <SUB>SAT</SUB> (d-prime satisfaction), is suggested. This new measure is compared to the comparative distance measure of <I>d'</I>, the degree of satisfaction difference of a product from the reference product, which has been already described in the previous study. The absolute satisfaction degree is a value indicating each product’s degree of consumer satisfaction as in the hedonic score. However, the degree of satisfaction measure presented herein is superior to the hedonic score because it enables easy interpretation of whether a group of consumers are satisfied or not with a product. In practical situations, the two measures of the degree of satisfaction difference from the reference and the degree of satisfaction, which are computed using the data from DOSD method, can be used complementarily in order to reveal information about consumer perception and evaluation.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Signal detection analysis applied for degree of satisfaction-difference (DOSD) data. </LI> <LI> A complimentary SDT analysis developed to broaden the interpretation of DOSD data. </LI> <LI> The novel output measure from this new analysis is referred to as <I>d'</I> <SUB>SAT</SUB>, d-prime satisfaction. </LI> <LI> Comparisons and how to interpret these two different analyses provided. </LI> </UL> </P>

한국인에서 혈소판 당단백 Ⅱb/Ⅲa 유전자 다형성과 관동맥 성형술 후 재 협착과의 관계

이민수,이정우,김보영,임대승,강정아,김정희,김윤철,성보영,최성준,성인환,전은석 충남대학교 의과대학 지역사회의학연구소 2000 충남의대잡지 Vol.27 No.2

Platelet aggregation is the final pathway of acute coronary syndrome such as acute myocardial infarction and unstable angina. Platelet glycoprotein IIb/IIIa is a membrane receptor for fibrinogen and yon Willebrand factor and it plays an important role in platelet aggregation and in the pathogenesis of acute coronary syndrome. It is known that polymorphism of the gene that encoding platelet glycoprotein IIb/IIIa(PI^A1/A2) is strongly related to acute coronary syndrome in Caucasian, but not in Koreans. We investigated relationship between platelet glycoprotein llb/Illa gene polymorphism and restenosis of coronary artery after angioplasty in Koreans. Total 371 patients(M=251. F=120) were enrolled. Angioplasty group comprised 143 patients who underwent coronary angioplasty, and in the angioplasty group, restenosis group comprised with the 65 patients who had restenotic lesion over 50% of luminal diameter in follow-up coronary angiography. Normal group comprised 153 patients who had no significant angiographic lesion and variant angina group comprised 75 patients who were positive in ergonovine test. Genomic DNA was extracted from peripheral arterial blood. To determine the frequency of P1^A1/A2 genotype, polymerase chain reaction(PCR) was done and the product was restricted with Mspl. 3%. agarrose gel electrophoresis showed restriction fragment length polymorphism. Clinical profile and risk factor were also reviewed. Among all 371 patients of study group, genotype of only one patients in restenosis group if is proven to be PI^A1/A2 heterozygote. All patients of normal study group, no restenosis group, and the other patients in restenosis group have an PI^A1 homozygote genotype. In our study, platelet glycoprotein IIb/Illa polymorphism has no relationship with restenosis of the coronary artery after angioplasty in Koreans. But the genotypic frequency of platelet glycoprotein IIb/IIIa gene polymorphism in Koreans is concordant with that of previous studies.