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Contractile Force Measurements of Cardiac Myocytes Using a Micro-manipulation System
Sukho Park,Seokkyu Ryu,Seokchang Ryu,Deokho Kim,Byungkyu Kim 대한기계학회 2006 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.20 No.5
In order to develop a cell based robot, we present a micro-mechanical force measurement system for the biological muscle actuators, which utilize glucose as a power source. The proposed measurement system is composed of a micro-manipulator, a force transducer with a glass probe, a signal processor, an inverted microscope and video recording system. Using this measurement system, the contractile force and frequency of the cardiac myocytes were measured in real time and the magnitudes of the contractile force of each cardiac myocyte under different conditions were compared. From the quantitative experimental results, we could estimate that the force of cardiac myocytes is about 20~40 μN, and show that there are differences between the control cells and the micro-patterned cells.
Kim, Suyeob,Kwak, Seungjoo,Lee, Seokchang,Cho, Woo Kyung,Lee, Jungkyu K.,Kang, Sung Min The Royal Society of Chemistry 2015 Chemical communications Vol.51 No.25
<P>The non-biofouling properties of a zwitterionic sulfobetaine polymer surface were easily made attractive to bioentities (<I>e.g.</I>, proteins and cells) by metal–polyphenol coating, and spatio-selective functionalization of the zwitterionic polymer surface was achieved by using a soft lithographic technique.</P> <P>Graphic Abstract</P><P>The non-biofouling properties of a zwitterionic sulfobetaine polymer surface were easily made attractive to bioentities by metal–polyphenol coating. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c4cc08609k'> </P>