Aspergillus parasiticus가 몇가지 穀類에서 生成하는 Aflatoxin에 對하여

洪世永,金敎昌,金道榮 충북대학교 농업과학기술연구소 1986 農業科學硏究 Vol.4 No.-

In order to investigate the influence of temperature, moisture and culture time on the aflatoxins produced by Aspergillus parasiticus NHL 5243 on some grains,5 kinds of grain samples (rice, barley, wheat, corn and sorghum) were used. The different fractions of aflatoxin B1, B2, G1, and G2 were detected from 5 kinds of samples by TLC and HPLC. Fluorescence compounds showed blue (aflatoxin B1, B2) and blue green(aflatoxinG1, G2) under 365nm UV lamp, but all of them were changed to green-grey fluorescence by 50% H2SO4, soln. Spraying, and to pink(B1, B2) and blude (G1, G2) by p-anisaldehyde. Aspergillus parasiticus produced even more aflatoxins on grains tested under incubating at 25% moisture content level than 17%, 20%. No aflatoxin was detected at 17% in rice, without regard to any temperature and culture time. Aflatoxins were detected at 20℃, 20%, for 21 days, and at 30℃, any moisture content level (except17%) , for any incubation period. Samples at 30℃, were found more produced aflatoxins than at 20℃. Aflatoxin G1 was obtained more production than B1, B2 and G2 at any incubated conditions in all samples. Total amount of aflatoxins was 2454㎍/kg in rice, 1257㎍g/kg in barley, 986㎍/kg in wheat, 882㎍/kg in corn and 1483㎍/kg in sorghum under the same conditions(30℃, 25%, 21days). The greatest fields of aflatoxin were obtained in rice sample.

膿胸의 外科的療法

柳世英 醫藥係社 1955 綜合 醫學 Vol.12 No.11

흰쥐 肝臟의 組織發生에 관한 組織化學的 및 電子顯微鏡的 硏究

鄭鎬三,李圭植,張世榮 한양대학교 의과대학 1985 한양의대 학술지 Vol.5 No.1

Numerous workers have intensively studied for physological and histochemical aspects on the histogenesis and differentiation of mammalian livers focusing attention on its hematopoietic function. In early fetal life hepatic diverticulum formed caudal part of the foregut in separated into two parts by the mesodermal septum each being developed into primordia later. The author has undertaken histological and histochemical studies to persue the morphological and functional changes to occur in hepatic lobules during development and differential period. The pregnant albino rats, Wistar strain, were sacrificed every day starting from 15th to 20th day of gestation. The specimens obtained from the fetal liver were fixed with 10% formalin and stained with hematoxylin-eosin to observe the morphological development and differentiation of the liver. The activities of acid and alkaline phosphatase were observed by the Gomori's methods(1931, 1941) and ATPase activity by the Wachstein-Meisel's method(1957). And also another liver specimens fixed with 4% glutaraldehyde in 0.1M cacodylate buffer(pH 7.4) at 4℃ by the Eranko's method(1952) were examined with electron microscope to investigate phosphatase activities in the hepatic parenchymal cells. The results were as follows. 1. In the liver of the 15th day of the fetus, undifferentiated hepatic parenchymal cells were constituted and a few nucleated erythocytes were spread at the intercellular region, and at the 16th and 17th day of the fetus a number of hematocytoblast were distributed and primitive hepatic cord and hepatic sinusoid were formed. 2. In the Liver of the 18th, 19th and 20th day of the fetus, hematopoietic cells were reduced but the differentiation of he hepatic parenchymal cells, hepatic cord and hepatic sinusoid were observed. 3. The activity of the acid phosphatase increased from the 17th to 20th day of the fetus. High activity of the enzyme at the peripheral and central zone and trace activity at the lysosome and rough endoplasmic reticulum were observed. 4. Moderate activity of the alkaline phosphatase was observed in the liver of the 19th and 20th day of the fetus at the nuclear membrane of the parenchymal cell and at the villi of the bile canaliculi. 5. The activity of the adenosine triphosphatase was observed in the liver of the 18th day of the fetus, and positive activity at the plasma membrane and weak activity at the rough endoplasmic reticulum were observed at the 19th and 20th day of the fetus, respectively.

흉부자상에 의한 심실중격결손 및 승모판 부전증 치험 1예

김범식,조순걸,곽영태,조규석,박주철,유세영 慶熙大學校 1985 論文集 Vol.14 No.-

A 34-year-old man was stabbed with knife in the left fourth intercostal space. He was admitted to Kyung Hee University Hospital Emergency room with Cardiac tamponade state. Through a left anterior thoracotomy, a tense hemopercardium was released. A 2㎝ wound of right ventricle was sutured with 3-0 prolein. Seven month after the injury he was readmitted for cardiac catheterization. Cardiac catheterization confirmed the presence of an interventricular septal defect in the membranous septum with left ventricle to right ventricle shunt. A laceration of the septal leflet of mitral valve was also demonstrated. The pulmonary blood flow as 1.22 times the systemic flow. A longitudinal incision was made on the right atrium on the cardiopulmonary bypass. An 1×0.5㎝ interventricular septal defect was found in the membranous septum. In order to achieve and adequate repair interventricular spetal defect and mitral insufficency. The ventricular septal defect was repaired 3-0 prolein plegeted suture with interrupted. Artifical atrial septal defect was made and the septal leflet defect(1.5㎝) was reattached with 3-0 prolein plegeted suture interrupted. The partient had and uneventful post operative recovery and was discharged the Hospital on the 24day post operative day.

Valinomycin Ionophore를 이용한 Potassium 이온 선택성 Poly(aniline) Solid Contact Electrodes에 관한 연구

박세연 ( Seh Youn Park ),오진주 ( Jin Joo Oh ),박소영 ( So Young Park ),권영순 ( Young Soon Kwon ) 한국환경분석학회 2003 환경분석과 독성보건 Vol.6 No.1

핵심지식 보유의 딜레마 : 스마트폰 산업에서의 오픈 OS 등장의 의미

박세영(Seh-Young Park),김영진(Young-Jin Kim) 한국전략경영학회 2010 한국전략경영학회 학술대회발표논문집 Vol.2010 No.4

특정 산업의 제품아키텍처를 구성하는 핵심지식은 핵심지식을 보유한 기업뿐 아니라 이를 활용해야 하는 산업내 참가자들에게도 중요한 의미를 가진다. 본 연구에서는 스마트폰 산업에서 핵심지식으로 규정될 수 있는 운영체제(OS) 영역을 바탕으로 핵심지식을 세분화하고 이를 다시 폐쇄형 핵심지식에서 개방형 핵심지식으로의 기술 환경변화의 의미와 연관지어 완제품메이커인 휴대폰업체의 대응방식 변화를 설명하고자 하였다. 연구대상 핵심지식 보유 유무에 따른 오픈 OS의 채용을 공통적으로 경험한 휴대폰 업체를 선택하였으며, 이를 통해 분석 틀과 관련된 2/2 매트릭스를 개발하였다. 본 연구는 핵심지식의 중요성에도 불구하고, 핵심지식 축적의 중요성이 약화되는 상황을 개방형 OS의 등장과 관련된 기술적 맥락에서 해석하고, 이러한 맥락에서 핵심지식의 사용자인 휴대폰업체는 상황변화에 따라 어떤 기술전략을 선택할 수 있는지 사례를 통해서 확인하였다. 결과적으로, 스마트폰과 같은 기술적 복잡성이 요구되는 세분화된 산업으로 이동시, 상황 변화에 따라 핵심지식의 축적과 사용 방식의 재검토가 요구된다는 것을 알 수 있다. The core knowledge what is composed of product architecture in a specific industry has very important meaning to which company has it as well as which companies have to utilize it in same industry. In this research, we segmented the core knowledge base on operating system(OS) in smartphone industry what can be considered the core knowledge. And, we try to explain how handset maker who is making a system product changes the strategy what is link to the meaning of technology environment changing from closed core knowledge to open core knowledge. The researching target companies were selected by the smartphone's main player. The sampled mobile phone companies were selected based on the existence of core knowledge retention which was commonly experienced with Open OS. And, for this analysis, we created the analyzing tool like 2/2 matrix. In this research, we analyzed the tendency of weakening for the core knowledge accumulation even the important is increased in terms of technical point of view in the context of technology. And, we checked what kinds of strategies can be selected by handset maker who is using core knowledge followed by industry environment changing through case studies. In conclusion, when it moved to new specified industry and needed technology complexity such as a smartphone, we could see that it is needed to review the core concept's accumulation and application strategy in according to the change of industry environment.

末梢動脈移植에 關한 實驗的硏究

柳世英(Seh Young Yoo) 대한외과학회 1971 Annals of Surgical Treatment and Research Vol.13 No.10

말초동맥손상(22례 수술보고)

유세영(Seh Young You),유영진(Young Jin You),이영균(Young Kyoon Lee) 대한외과학회 1970 Annals of Surgical Treatment and Research Vol.12 No.1

리놀레산에 의한 인체암세포의 성장 억제효과 및 암세포막 인지질 지방산 변화

임선영(Sun-Young Lim),이숙희(Sook-Hee Rhee),이세윤(Seh-Yoon Yi),박건영(Kun-Young Park) 한국식품영양과학회 1997 한국식품영양과학회지 Vol.26 No.4

MG-63 골육암세포 및 AZ-521 인체 위암세포 성장에 미치는 리놀레산(LA)의 성장 억제효과와 이때 암세포막의 인지질 유리지방산 조성 변화에 대한 연구를 하였다. LA 첨가 농도 0.005%에서 MG-63 암세포와 AZ-521 암세포는 대조군에 비해 각각 54% 및 52%의 암세포 성장이 억제되었다. 또한 LA 첨가(0.01%)시 이들 암세포의 [³H] thymindine 결합능은 90% 이상이 억제되어 암세포의 DNA합성이 크게 억제되었다. 한편 LA(0.005%) 첨가시 각 암세포의 형태학적인 변형을 유발시켰으며 세포막의 인지질 지방산 조성의 변화가 관찰되었다. LA처리는 이런 세포의 형태적 변형과 함께 세포막의 인지질 중 16 : 0과 18 : 0의 지방산은 현저히 감소되었으나 탄소수가 20 이상의 탄소수를 가지거나 불포화도가 높은 지방산(20 : 4, 22 : 6, 24 : 4)은 증가되었다. Linoleic acid(LA) was examined to evaluate its potential as a chemotherapeutic agent for MG-63 human osteosarcoma and AZ-521 gastric cancer cells. The treatment of LA(0.005% for 6 days) to the MG-63 and AZ-521 cancer cells inhibited growth of the cancer cells by 54% and 52%, respectively as compared to that of the controls. It also exhibited that LA with 0.01% concentration decreased the [³H] thymidine incorporation by more than 90% in the both cancer cells. In additions we observed morphological changes in MG-63 and AZ-521 cells under inverted microscope, and the changes in membrane fatty acid compositions of the cancer cells when LA was added at the level of 0.005%. The treatment with LA revealed that the contents of 16 : 0 and 18 : 0 decreased significantly, but fatty acids that C numbers are more than 20 and unsaturated(20 : 4, 22 : 6, and 24 : 4) increased, concomitantly the morphological changes of the cells were observed.

廻腸을 利用한 結核性 萎縮膀胱의 擴大成形術에 關한 治驗例

宋運永(Woon Young Song),李會白(Hoi Paik Lee),金世煥(Seh Whan Kim),Robert F. Roth,金榮祐(Young U Kim) 대한외과학회 1968 Annals of Surgical Treatment and Research(ASRT) Vol.10 No.7