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( Seetha Ramulu J. ),( Raja Gopal Reddy C. ),( R. Ramanjaneyulu ) 한국잠사학회 2010 International Journal of Industrial Entomology Vol.20 No.1
The studies have been conducted to control the soil borne fungal pathogens viz, Fusarium solani (Mart) Sacc. and Alternaria tenuissima the incitants of root rot and die-back diseases on mulberry stem cuttings planted in the mulberry nurseries and also in established mulberry gardens ten plant extracts with 10% concentration except Lantana camara (undiluted) were tested through poisoned food technique and four biofungicides were also screened by dual culture method under in vitro conditions. Plant extract of Prosopis juliflora showed the maximum inhibition on the mycelial growth (81.2% over A. tenuissima and 80.0% over F. solani) and followed by L. camara (66.7% over A. tenuissima and 68.9% over F. solani). Among the antagonists Pseudomonas fluorescens and Trichoderma viride showed maximum inhibition on the mycelial growth of both pathogenic fungi. The promising plant extracts (P. juliflora and L. camara) and antagonists (P. fluorescens and T. viride) were tested against both the pathogenic fungi under in vivo conditions along with the existing popular chemical Mancozeb. All the tested plant products and bio-fungicides showed inhibitory effect on both fungi. But the maximum survival percentage of mulberry cuttings was recorded in the treatment with T. viride (95% against F. solani and 90% against A. tenuisssima) followed by P. fluorescens (90% against both fungi) and T. harzianum (80% against F. solani and 85% against A. tenuisssima). Incase of the treatments with plant extracts and chemical fungicide the P. juliflora (60% against F. solani and 55% against A. tenuisssima) showed higher survival percentage and followed by L. camara (55% against F. solani and 50% against A. tenuisssima) and Mancozeb (55% against both fungi). In case of control only 10% of survival was recorded in F. solani inoculated cuttings and 15% survival in A. tenuissima inoculated cuttings.
Supakorn Seetha,Rungarune Saymung,Rakchart Traiphol,Nisanart Traiphol 한국공업화학회 2019 Journal of Industrial and Engineering Chemistry Vol.72 No.-
Polydiacetylene/zinc(II) ion/zinc oxide (PDA/Zn2+/ZnO) nanocomposite exhibits reversible thermochromismand dual colorimetric response to acid/base. This contribution presents our ongoingdevelopment of the PDA/Zn2+/ZnO nanocomposite for sensing applications by controlling ZnO surfacecharge and dissociation of PDA headgroup via pH adjustment. At pH >10, negative ZnO surface charge andPDA carboxylate headgroup significantly enhance molecular organization during the self-assemblingprocess. An increase of the nanocomposite amount after photopolymerization is observed. Oppositely,pH <6 results in irreversible-thermochromic nanocomposites. Additionally, the nanocompositesprepared at different pH change color at different concentrations of chemical stimuli. Molecularpacking, local interactions and PDA conformation are investigated.
K. Seetha Ram,K. Satish Babu,G. Tabitha,K. Rajeshwari,G. Jaya Lakshmi,B. Boje Gowd,J. B. Peravali,M. Subba Rao,P. Venugopala Rao 보안공학연구지원센터 2015 International Journal of Bio-Science and Bio-Techn Vol.7 No.6
Most of the bacterial and other simple non glycosylated recombinant proteins were conventionally produced from IPTG inducible Escherichia coli BL21(DE3). Considering the factors like cost and toxic nature of IPTG, as an alternative, salt inducible Escherichia coli GJ1158 was used in this study for the over production of staphylokinase variant (sak – hirulog) using fed batch fermentation, cost effectively. Optimization of physico chemical factors viz., dissolved oxygen (DO), carbon, nitrogen and phosphate sources on bacterial growth for the production of recombinant sak – hirulog using batch and fed batch fermentation was studied. In batch culture, increased DO at more than 30 % did not influence the enhanced expression of sak – hirulog, but significant improvement was observed in fed batch cultivation. Supplementation of production medium with different nutrient sources like dextrose, yeast extract and dipotassium hydrogen phosphate (K2HPO4) enhanced the sak – hirulog expression in fed batch fermentation process even without disturbing the cell growth by providing 50 % DO. Approximately 1178 mg/L of specific protein was obtained using cost effective modified glucose yeast exgtract (GYE) media devoid of sodium chloride. This study also reports the highest concentration of recombinant protein from salt inducible expression host till to date, which manages to satisfy the production of bifunctional staphaphylokinase variant using economically feasible bacterial expression host Escherichia coli GJ1158.
A Software Safety Model for Safety Critical Applications
M. Ben Swarup,P. Seetha Ramaiah 보안공학연구지원센터 2009 International Journal of Software Engineering and Vol.3 No.4
Software has become responsible for most of the critical functions of complex systems. Software safety is the notion that software will execute within a system context without contributing to hazards. Software for safety-critical systems must deal with the hazards identified by safety analysis in order to make the system safe. Software safety is a composite of many criteria. Existing software quality models like McCall’s and Boehm’s and ISO 9126 are inadequate in addressing the software safety issues of real time safety-critical embedded systems. At present there does not exist any standard model that comprehensively addresses the factors, criteria and metrics (FCM) approach of the quality models in respect of software safety. This paper proposes a new model for software safety based on the McCall’s software quality model that specifically identifies the criteria corresponding to software safety in safety critical applications. This framework is then applied to a prototype safety-critical system viz. a software?controlled Road Traffic Control System (RTCS) commonly used in city traffic, to validate its utility.