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Monica John Sedere 이화여자대학교 아시아여성학센터 2017 이화여자대학교 아시아여성학센터 학술대회자료집 Vol.2017 No.7
Every human being is entitled to clean, safe, affordable and physically accessible water. However, the availability and accessibility of safe and clean water is the challenge to citizens, especially in poor countries, Tanzania inclusive. Besides, the hassle is much worse to women and girls, mainly those living in rural areas. This paper will explore the limitations of women and girls to enjoy their right to water and sanitation in schools, health facilities and at home. Among others, the paper will also link the lack of water and poor sanitation to women`s poverty and the national economic loss. Conclusively, the paper will recommend a collective voice and action in demanding for adequate resource allocation for improving the provision of water and sanitation in order to empower women and girls socially, economically and politically in one way and improving the national economy on the other hand.
Microfluidic chip with movable layers for the manipulation of biochemicals
Seder, Islam,Kim, Dong-Min,Hwang, Sang-Hyun,Sung, Heungsup,Kim, Dong-Eun,Kim, Sung-Jin The Royal Society of Chemistry 2018 Lab on a chip Vol.18 No.13
<P>A simple and effective platform that can conglomerate various microfluidic functions in a single chip is essential for many bioassays, especially for point-of-care testing applications. Here, a chip that exploits surface tension in solutions with movable top and bottom layers is presented, for use in fluid transport, mixing, maintaining metered volumes, and biomolecule capture and release. The chip has open chambers in vertically mobile top layers and rotationally mobile bottom layers to exploit surface tension in biochemical solutions, and implements control over fluid motion. To manipulate biomolecules, a vertically mobile tip with a permanent magnet at the top layer performs collection, transport, release, and dispersion of magnetic beads. Thus, the chip orchestrates various fluidic control functions without using on-chip valves and pumps that increase operational and structural complexity. To demonstrate its utility, the chip performs automated DNA extraction by obtaining genomic DNA from a sample containing cells. Our approach provides a useful and effective alternative to numerous platforms that use active and passive on-chip components for bioassays.</P>
Comparison of preservation enrichment media for long storage duration of Campylobacter jejuni
AL-Fawares O'la,Al-Khresieh Rozan O.,Aburayyan Walid,Seder Nesrin,Al-Daghistani Hala I,El-Banna Nasser,Abu-Taleb Emran M. 한국미생물학회 2023 미생물학회지 Vol.59 No.3
Research on Campylobacter jejuni has been greatly impaired due to poor culturing and storage techniques. This study aims to evaluate different preservation methods for C. jejuni for long-term periods of preservation. Three different preservation methods (i, ii, and iii) were tested for their potential for long-term storage of C. jejuni which could be recommended as a simple and reliable method. In the first preservation method (i), 15% glycerol was added to 85% of the bacterial suspension of 4 McFarland standards (McF) and yeast extract. In the second method (ii) the bacterial suspension was supplemented with defibrinated lacked horse blood along with skim milk. Skim milk and 50% glycerol were added to the bacterial suspension in method (iii). Results indicated that method (ii) maintained the highest preservation rate of C. jejuni and the bacteria was capable to grow even after 12 months of storage at -80°C. Method (i) was less efficient than method (ii) where isolates eventually lost their ability to grow after 8 months. Method (iii) showed the least successful preservation rate as many isolates started to die after six months of preservation. This study emphasizes the importance of using defibrinated lacked horse blood along with skim milk during the preservation of bacterial isolates of C. jejuni.