상아질 접착에서 collagenase와 esterase가 미세인장결합강도에 미치는 영향

정영정,현홍근,김영재,김정욱,이상훈,김종철,한세현,장기택 대한소아치과학회 2007 大韓小兒齒科學會誌 Vol.34 No.2

상아질-레진 접착강도에 대한 collagenase와 esterase의 영향을 살펴보기 위해, 소구치의 교합면 상아질에 Single Bond 2와 Clearfil SE Bond로 접착을 시행하고 미세 시편을 제작하여 PBS, collagenase 용액, esterase 용액에 4주간 보관한 후 미세인장결합강도를 측정, 비교하여 다음과 같은 결론을 얻었다. 1. 모든 보관 용액에서 Single Bond 2의 미세인장결합강도는 Clearfil SE Bond보다 유의하게 낮았다(p<0.05). 2. Single Bond 2의 미세인장결합강도는 collagenase군이 PBS군, esterase군보다 낮았다(p>0.05). 3. Clearfil SE Bond의 미세인장결합강도는 esterase군이 PBS군에 비해 낮았으나(p>0.05), collagenase군보다는 높았다(p>0.05). Collagenase군은 PBS군에 비해 유의하게 낮은 미세인장결합강도를 보였다(p<0.05). The purpose of this study was to evaluate the effect of collagenase and esterase on the microtensile bond strength (μTBS) in dentin bonding. After resin composites were bonded to occlusal dentin, μTBS specimens were formed and stored in PBS, collagenase, or esterase solution. After 4-week storage, μTBS was determined and, the results were as follows: 1. μTBS values of Single Bond 2 were lower than those of Clearfil SE Bond for all storage medium (p<0.05). 2. In single Bond 2 group, collagenase solution lowered bond strength more than PBS and esterase solution (p>0.05). 3. In Clearfil SE Bond group, esterase solution lowered bond strength more than PBS(p>0.05). Collagenase solution lowered bond strength more than esterase solution(p>0.05) and PBS(p<0.05).

무산소성 운동시 혈중 젖산농도 변화에 관한 연구

이근세 대구산업정보대학 2000 논문집 Vol.14 No.2

The purpose of this study was to determine anaerobic peak power, mean power, Vo2max, VE, R.E.R and blood lactate levels in non-athletes, Ssirum and physical education groups during wingate anaerobic test. 2lmales, aged 19-27 years performed the Wingate test. The results are as follows : 1. There were significant differences in peak power and mean power between the groups(p<.01). 2. There were significant differences in Vo2max and VE between the groups(p<.05). 3. There were not significant differences in R.E.R and blood lactate between the group.

응급실에 내원한 약물중독환자의 지역별 비교분석

임근우,박일영,유승진,나병호,오동렬,전해명,황주일,김세경 대한응급의학회 1995 대한응급의학회지 Vol.6 No.1

We reviewed retrospectively 230 patients who came to emergency room of Kang Nam St. Mary's Hospital and Uijongbu St. Mary's Hospital between 1. January and 31. December 1994. We classified them according to their age, sex, a sort of drug, the cause of poisoning, prehospital care, time-lapse to arrive at hospital after poisoning, psychiatric problem the status of patients. *We conclude as follows; 1. The male to female ratio was totally 1 : 1.98, 1 : 1.24 in a urban area, 1 : 2.43 in a rural area. 2. On age of the patients in this study, the peake incidence was between 20 and 39(55.2%). 3. On seasonal prevalence, the poisoning was most common in summer and spring, in urban area(35.7%) and rural area(37.0%) respectively. 4. The common drug were as follow; Doxylamine, Organophosphate, Acetaminophen in urban area, Paraquat, Organophospate, Doxylamine in rural area 5. The percentage of suicidal attempt were 81.8% and 87.7% in urban area and rural area respectively. 6. The poisoning patients had psychatric problem in 19.4% and 24.6%, in urban area and rural area respectively. 7. Before arrival to emergency room, 27.7% of patient was done prehospital care in rural area. 8. On arrival to emergency room, 95.1% and 73.8% of patients show stable vital sign in urban area and rural area respectively.

고령토로부터 알루미나 추출에 관한 연구(Ⅲ):염산에 의한 추출

최세영,이용근,이희수,김형국 연세대학교 산업기술연구소 1992 논문집 Vol.24 No.1

The preparation conditions of alumina powder extracted by hydrochloric acid from domestic San-Cheong kaolin have been investigated. In order to obtain best conditions to extract alumina by hydrochloric acid, experiments carried out with various acid concentrations, reaction temperatures, reaction times, and pH. The optimum conditions were 20 wt.-% for hydrochloric aicd concentration, 100℃ for reaction temperature, 7hrs for reaction time and pH8. The yield of alumina found to be 89.2wt.-% under these conditions. Two drying methods, direct and ethanol distillation drying method to control the precipitated agglomeration have deen compared. The ethanol distillation drying method revealed more narrow particle size distribution and less agglomerate than the direct drying method.

한국인 강박장애와 Val-158-Met Catechol-O-Methyltransferase 유전자 다형성 간의 상관성 연구

천근아,김세주,김찬형,구민성,남윤영,박성혁,이홍식 大韓神經精神醫學會 2006 신경정신의학 Vol.45 No.5

목적 COMT(Catechol-O-Methyltransferase) 효소는 도파민성과 노어아드레날린성 체계의 조절에서 매우 중요한 작용을 하며 염색제 22q11에 위치해 있다. 일부의 강박장애 환자들은 도파민 차단제에 좋은 치료 반응을 보이며 이런 현상은 COMT가 강박장애의 병인론에 역할을 하고 있을 가능성을 말해준다. Val-158-Met COMT 유전자의 다형성은 효소의 감소를 유도하는 L(저활성) 대림형질, 활성의 증가를 유발하는 H(고활성) 대립형질로 알려져 있으며 L 대립형질 또는 LL 유전형이 강박장애와 연관이 있다는 보고가 있으나, 아직까지 강박장애와 COMT 다형성 사이의 연관성에 대한 연구결과들은 일관되지 않다. 따라서 본 연구에서는 한국인 강박장애 환자들을 대상으로 Val-158-Met COMT 유전자의 다형성과의 연관성을 알아보고자 하였다. 방법 2002년 3월부터 2004년도 2월까지 약 24개월동안 서울지역 일 대학병원과 경기지역 일 대학병원 강박장애 클리닉을 방문하여 강박장애로 진단받은 124명의 환자들을 연구대상으로 하였고 정상 대조군으로는 나이와 성별을 고려하여 자원자 170명이 선별되어 포함되었다. 강박장애군과 정상 대조군의 혈액으로부터 유전자 추출 후 COMT 유전자 형벌 (genotyping)을 시행하였다. 다형성을 H/H 와 H/L, L/L 유전형으로 나눈 뒤 강박장애와 정상대조군 사이의 유전자 빈도를 카이 검정법으로 비교하여 연관성을 관찰하었다. 또한 강박장애 환자들에게 시행한 YBOCS로부터 측정한 강박장애 심각도 및 Hamilton Depression Raing Scale (HDRS) 총점과 COMT 유전자 다형성 사이의 연관성을 비교하였다. 결과 본 연구 결과 한국인 강박장애와 COMT 다형성 간에는 유의한 상관성이 관찰되지 않았다. YBOCS 심각도와 COMT 유전자형과의 유의한 연관성이 관찰되지 않았다. 그러나, 강박장애 환자군들의 COMT 유전자형 세 군 사이의 우울증상 점수에서는 유의한 차이가 관찰되었다. 결론 한국인에서 강박장애와 COMT 유전자 다형성 사이에는 연관성이 없었다. 그러나, COMT의 H 대립형질과 강박장애 군에서 공존하는 우울증상 척도가 유의한 연관성을 보인 현상은 COMT 유전자의 다형성이 강박장애의 공존 우울증상에 영향을 미칠 수 있는 가능성을 시사한다고 생각된다. Objectives : The definite cause of obsessive-compulsive disorder (OCD) is still unknown. Evidences from familial, twin and segregation studies support the role of a genetic factor. There are also growing evidence indicating that OCD has specific neurochemical and neuroanatomical basis. Derived from the effectiveness of treatment with dopamine receptor blocker in certain part of OCD patients (eg. SSRI treatment-resistant OCD), several candidate genes related to dopamine dysregulation have been hypothesized to play an important role in the development of OCD. One of them is the Catechol-O-Methyltransferase (COMT) gene. The aim of this study was to investigate the association between COMT and OCD in Korean population. Methods : 124 OCD patients and 170 normal controls participated in this study. Genomic DNA was extracted from their blood. The genotypes and allele frequencies of the COMT polymorphism between OCD group and control group were compared. We investigated the association between severity of OCD assessed by Yale-Brown obsessive compulsive symptom scale (YBOCS) and Hamilton depression rating scale (HDRS) scores and COMT polymorphism. Results : In this case-control study, we could not find any association between COMT gene polymorphism and development of OCD. In OCD group, patients with H/H genotype had significantly higher scores for the HDRS than those with H/L or L/L genotype. Conclusion : In this study, there was no difference in genotype distributions of COMT between OCD and control groups. However, H/H genotype of COMT gene polymorphism might be related to depressive symptoms in OCD patients.

21세기의 초등학교 무용교육방향에 관한 연구

김세정,이근모 釜山大學校 師範大學 1998 교사교육연구 Vol.35 No.-

This study is aimed at objectifying several procedures for desiable direction of basic dance instruction. The procedures are first, consideration of 〈Journal of Korean Elementary school Dance Education Society〉 to seek current goal and emphasized point of dance instruction. Second, revealing educational value different from that of established by studying realistic and axiom definition of dance. Third, seeking education requirement necessary for fostering as desirable social component necessary for future by arrangement of opinion of future scholar to measuring desirable direction of 21century. Finally, predicting the desirable direction of 21century by these considerations mentioned previously. The result is that the direction of future dance education to foster desirable human for 21century should not emphasize only the body, creation, activity, etc emphasized as current motion education and inspiration of union consciousness should be include folk dance able to inspire union consciousness.

PelB Signal Sequence로 유도된 제조합 인간 상피세포 증식인자 분비 발현 벡터의 제조

박세철,남정현,김정근,권태종,고인영,유광현 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.5

전사개시 단계중에 삽입되는 N말단의 methionine이 제거되고 pelB signal sequence를 삽입하여 인체 epidermal growth factor (hEGF)가 직접 분비발현 되도록 고안된 T7과 tac promoter에 의하여 각각 발현되는 pYHB101과 pYHB2를 제조하였다. 또한 T7 promoter에 의하여 발현되지만 signal sequence를 삽입하지 않은 pYHB1도 제조하여 각각 제조된 재조합 plasmid들의 발현 및 분비효율을 비교하였다. pelB signal sequence hEGF 유전자를 갖도록 제조된 pYHB101을 포함하고 있는 균주가 pYHB1보다 균체 성장속도는 낮았지만 pYHB101을 1 mM IPTG로 5시간 induction 하였을 때 13 mg/l의 rhEGF가 분비발현되어 T7 promoter의 발현 체계를 갖는 경우가 tac promoter보다 높은 발현 효율을 보였다. 이때 pYHB101에서 발현되는 rhEGF의 발현량은 준최적 조건에서 14 mg/l이었다. We have designed nucleotide sequences of hEGF structural gene to eliminate the N-terminal methionine residue incorporated during the translation initiation step, and constructed recombinant human epidermal growth factor (rhEGF) secretion plasmids pYHB101, and pYHB2 in which pelB signal sequence-hEGF gene was expressed under the control of the T7, and tac promoter, respectively. We also constructed pYHB1 vector which contains rhEGF gene controlled by T7 promoter. The transformant with pYHB101 showed relatively slow growth pattern compared to the transformant with pYHB1. However, we observed that the transformant with pYHB101 secreted rhEGF of 13 mg/l significantly after 5 hr induction with 1 mM IPTG and that the T7 promoter was more effective than tac promoter when connected to pelB signal sequence. The amount of rhEGF was 14 mg/l under the sub-optimized condition.

구원과 행동 : 철학과 종교의 관계에 관한 고찰 On the Relationship Between Philosophy and Religion

이근세 경희대학교 인문학연구소 2003 인문학연구 Vol.7 No.-

In this paper, I inquire into the relationship between philosophy and religion in the perspective of Maurice Blondel who inaugurated the philosophy of action. This study is based on the fact that these two domains try of determine how the human being acquires emancipation(i.e salvation) and to universalize this determination. Blondel's phenomenology of religion is intended to overcome two doctrines; immanentism (doctrine of immanence) and timid transcendentalism. The former makes exclusive distinction between reason and belief to give priority to reason. The latter insists that region of religion can never be understood by reason. According to Blondel's phenomenology of religion, religious requests of man can be defined by reason and philosophy is not able to determine the contents of religion but to describe the forms of that. So, considering the religion not as a problem to solve but as the obligation to practice, philosophy expands its realm.

미니홈페이지를 통한 글쓰기 교육이 작문에 미치는 영향

박세욱,한건우,이은경,이영준 한국교원대학교교육연구원 2006 敎員敎育 Vol.22 No.4

濟州道 住民 蟯蟲感染의 疫學的 硏究

李根泰,李範珪,盧英俊,任世旭,李在興 醫藥係社 1955 綜合 醫學 Vol.12 No.12