The Effect of Topiramate on Hippocampal Neuronal Death and Expression of Glutamate Receptor in Kainate-induced Status Epilepticus Model

박민정,하세운,배혜란,김상호,Park Min-Jeong,Ha Se-Un,Bae Hae-Rahn,Kim Sang-Ho Korean Society of Life Science 2005 생명과학회지 Vol.15 No.3

신경흥분독성과 간질발작발현은 glutamate 수용체활성과 연관이 있다고 알려져 있다. a-amino-3-hydroxy-5-methyl-4-isoxazole-propionate (AMPA), kainate 수용체에 대한 glutamate 활성을 포함하는 다양한 기전을 가진 항전간제인 Topiramate는 신경보호작용을 가진다는 증거가 제시되어 Topiramate가 간질발작 후 해마의 glutamate 수용체 발현에 미치는 효과를 관찰하였다. 흰쥐에 kainate를 복강 내 주사하여 간질중첩증을 유발시킨 후 Topiramate를 1주일 주사하였다 Apop tag in situ detection kit를 이용하여 세포손상을 관찰한 결과 kainate 유발 간질중첩증 1주일 후 해마의 CA1, CA3에서 심각한 세포사를 보였으나, Topiramte 처리 군에서는 세포사가 현저히 감소하였다. 간질중첩증 이후 NMDA 수용체 아형 1,2a, 2b 발현이 현저히 증가했으나 Topiramate 처치에 의해 NMDA수용체의 발현에는 뚜렷한 변화가 없었다. AMPA수용체에서는 GluR1이 간질중첩증 이후 현저히 상향 조정되었고 GluR2는 현저히 하향조정 되었다 Topiramate 1주일 처리 시 간질중첩증으로 인해 변화된 CluR1과 GluR2의 발현이 역전되었다. 결론적으로 Topiramate는 간질중침증에 의한 CluR1/CluR2 발현비의 증가로 유발되는 흥분성 신경세포사를 억제시킴으로써 신경보호작용이 있는 것으로 보인다. Excitotoxicity and epileptogenesis have often been associated with glutamate receptor activation. Accumulating evidences indicates that topiramate (TPM), an antiepileptic drug with multiple mechanisms of action has neuroprotective activity. We explored the neuroprotective effect of TPM on the status epilepticus (SE)-induced hippocampal neuronal death. After development of SE by kainite injection (15 mg/Kg), rats were treated with TPM (10mg/kg) for 1 week. The neuronal death was detected by Apop tag in situ detection kit, and the expression levels of glutamate receptors were semi-quantitatively analyzed by immunoblot. Kainate-induced SE caused a significant neuronal death and cell loss in CAI and CA3 regions of hippocampus at 1 week. However, treatment of TPM for 1 week after SE markedly reduced hippocampal neuronal death. The expression of N-methyl-D-aspartate (NMDA) receptor subunit 1, was increased by SE, but was not affected by 1 week treatment of TPM. The expressions of NMDA receptor subunit 2a and 2b were not changed by either SE or TPM. As for ${\alpha}-amino-3-hydroxy-5-methyl-4-isoxazole-propionate$ (AMPA) glutamate receptors (GluR), kainate-induced SE markedly up-regulated GluR1 expression but down-regulated GluR2 expression, leading to increased formation of $Ca^{2+}$ permeable GluR2- lacking AMPA receptors. TPM administration for 1 week attenuated SE-induced expression of both the up-regulation of GluR1 and down-regulation of GluR2, reversing the ratio of GluR1/GluR2 to the control value. In conclusion, TPM protects neuronal cell death against glutamate induced excitotoxicity in kainate-induced SE model, supporting the potential of TPM as a neuroprotective agent.

A Comparative Study of Nanoparticle-Ink-Based CIGSSe Thin Film Solar Cells on Different Back Contact Substrates

Se Jin Park,Jin Woo Cho,Gi Soon Park,Jae Seung Jeong,김지현,고두현,황윤정,민병권 대한화학회 2016 Bulletin of the Korean Chemical Society Vol.37 No.3

The effect of the back contact electrode in nanoparticle-ink-based CIGSSe solar cells was investigated using different glass substrates with either a fluorine-doped SnO2 (FTO) conducting layer or a molybdenum (Mo) conducting layer. The morphologies, crystal structures, and compositional distributions of the Cu(In x Ga1– x )(S y Se1– y )2(CIGSSe) films are very similar in spite of the different back contact materials. The observed performances of the solar cell device, however, are somewhat different. The device that was fabricated on the FTO back contact substrate revealed a lower open-circuit voltage ( V oc) and fill factor (FF) than the Mo back contact substrate, resulting in lower solar cell efficiencies (6.5% for FTO and 7.4% for Mo). The differences between the behaviors are attributed to the interfacial properties between the CIGSSe film and the back contact electrode.

Chalcogenization-Derived Band Gap Grading in Solution-Processed CuIn_<i>x</i>Ga_1–<i>x</i>(Se,S)₂ Thin-Film Solar Cells

Park, Se Jin,Jeon, Hyo Sang,Cho, Jin Woo,Hwang, Yun Jeong,Park, Kyung Su,Shim, Hyeong Seop,Song, Jae Kyu,Cho, Yunae,Kim, Dong-Wook,Kim, Jihyun,Min, Byoung Koun American Chemical Society 2015 ACS APPLIED MATERIALS & INTERFACES Vol.7 No.49

<P>Significant enhancement of solution-processed CuInxGa1-x(Se,S)(2) (CIGSSe) thin-film solar cell performance was achieved by inducing a band gap gradient in the film thickness, which was triggered by the chalcogenization process. Specifically, after the preparation of an amorphous mixed oxide film of Cu, In, and Ga by a simple paste coating method chalcogenization under Se vapor, along with the flow of dilute H2S gas, resulted in the formation of CIGSSe films with graded composition distribution: S-rich top, In- and Se-rich middle, and Ga- and S-rich bottom. This uneven compositional distribution was confirmed to lead to a band gap gradient in the film, which may also be responsible for enhancement in the open circuit voltage and reduction in photocurrent loss, thus increasing the overall efficiency. The highest power conversion efficiency of 11.7% was achieved with J(sc) of 28.3 mA/cm(2), V-oc of 601 mV, and FF of 68.6%.</P>

영아 보육교사의 직무환경과 교사효능감의 관계

최정선 ( Choi Jeong-sun ),김세곤 ( Kim Se-gon ) 동국대학교 교육연구소 2019 교육연구 Vol.4 No.1

본 연구에서는 영아 보육교사의 직무환경과 보육 효능감 간의 관계를 알아보고자 하였다. 본 연구 대상은 부산광역시 소재 G군의 어린이집 50개소에 재직 중인 보육교사 300명이다. 설문지를 300부를 배포 한 후에 기관을 방문하여 그중 200부를 직접회수 하였다. 영아 보육교사의 직무환경을 측정하기 위하여 선행연구에 기초하여 검사 도구를 본 연구에서 보육교사용으로 문항 내용을 일부 수정, 보완하여 사용하였다. 본 연구를 위한 설문지 초안이 구성된 후, 예비조사를 거쳐 본 조사가 실행되었다. 수집된 자료는 SPSS 20.0을 사용하여 통계적 자료 처리를 실시하였다. 빈도분석, 기술통계분석, 신뢰도 분석, t-test, 일원변량분석, 상관분석 및 다중회귀분석을 실시하였다. 주요 연구결과는 다음과 같다. 첫째, 영아보육교사의 직무환경인식과 보육효능감은 보통보다 높았다. 둘째, 영아보육교사의 직무환경인식과 보육효능감 간에는 상관관계가 유의미하였다. 셋째, 영아보육교사의 직무환경 중 ‘시설장과의 관계’와 ‘교사전문성 지원’ 요인이 보육효능감에 유의미한 영향을 미쳤다. The purpose of this study is to find out relationship between early childhood teachers' job environment and child care efficiency. Subjects of this study are 200 early childhood teachers in 50 daycare-centers, G-gun, Busan. Jin-hwa Lee's research tool(2007) which was modified from Ji-young Seo's tool(2002) to apply to early childhood teachers was used in order to measure early childhood teachers' job environment. To measure child care efficiency, Jeong-ae Lee's tool(2012) which used a tool invented by Riggers Enochs(1990) was used after partial modifying. Collected data was processed statistically by using SPSS 20.0. Frequency analysis, descriptive statistical analysis, t-test, one-way analysis of variance, correlation analysis and multiple regression analysis were conducted. Research results about research questions are as follows. First, early childhood teachers' job environmental recognition and child care efficiency were higher than neutral. Second, interrelationship between early childhood teachers' job environmental recognition and child care efficiency had statistical significance. Third, "Relationship with a director of a daycare-center" and "support of teacher professionalism" among early childhood teachers' job environmental factors affected child care efficiency meaningfully.

Effects of Alkali Post-treatments on Heterointerface and Device Performances of Cd-free Flexible Cu(In,Ga)Se₂ Solar Cells

Awet Mana Amare,Inchan Hwang(황인찬),Inyoung Jeong(정인영),Joo Hyung Park(박주형),Soomin Song(송수민),Ara Cho(조아라),Jun-Sik Cho(조준식),Seung Kyu Ahn(안승규),Jinsu Yoo(유진수),Se Jin Ahn(안세진),Jihye Gwak(곽지혜),Donghyeop Shin 한국신재생에너지학회 2023 한국신재생에너지학회 학술대회논문집 Vol.2023 No.6

Ga 함량에 따른 Cu(In<SUB>x</SUB>Ga<SUB>1-x</SUB>)Se₂ 태양전지의 특성분석

김석기(Seok Ki Kim),권세한(Se Han Kwon),이두열(Doo Yeol Lee),이정철(Jeong Churl Lee),강기환(Ki Whan Kang),윤경훈(Kyung Hoon Yoon),안병태(Byung Tae Ahn),송진수(Jinsoo Song) 대한전기학회 1998 대한전기학회 학술대회 논문집 Vol.1998 No.7

체간안정화 훈련이 만성 뇌졸중 환자의 호흡기능에 미치는 효과

이경진(Kyeong-Jin Lee),정주현(Ju-Hyeoun Jeong),조명래(Myeong-Rae Jo),김세윤(Se-Yoon Kim),김난수(Nan-Soo Kim) 대한심장호흡물리치료학회 2019 대한심장호흡물리치료학회지 Vol.7 No.1

Purpose : The purpose of this study is to examine the effects of trunk stability training on improving trunk control, respiratory function, and respiratory muscle activation in stroke patients. Methods : The subjects were assigned to two groups: the intervention group (n=15) and the control group (n=15). Both groups participated in a conventional stroke rehabilitation program, but the intervention group also received trunk stability training for 30 minutes a day, 3 times a week, for 6 weeks. The main trunk stability exercise consisted of the dead bug exercise. Trunk control was assessed using a trunk impairment scale, and respiratory function (pulmonary function and respiratory muscle function) was assessed using spirometry. The data were analyzed using the Pearson correlation, independent t-test, and paired t-test. Results : Trunk control was significantly positively correlated with pulmonary function and respiratory muscle strength. Both groups showed a significant increase in trunk control and respiratory function. Conclusion : This study demonstrated that a conventional stroke rehabilitation program and trunk stability training have positive effects on respiratory function and respiratory muscle activation in stroke patients.

Association between Socioeconomic Status and Altered Appearance Distress, Body Image, and Quality of Life Among Breast Cancer Patients

Chang, Oliver,Choi, Eun-Kyung,Kim, Im-Ryung,Nam, Seok-Jin,Lee, Jeong Eon,Lee, Se Kyung,Im, Young-Hyuck,Park, Yeon Hee,Cho, Juhee Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.20

Background: Breast cancer patients experience a variety of altered appearance - such as loss or disfigurement of breasts, discolored skin, and hair loss - which result in psychological distress that affect their quality of life. This study aims to evaluate the impact of socioeconomic status on the altered appearance distress, body image, and quality of life among Korean breast cancer patients. Materials and Methods: A cross-sectional survey was conducted at advocacy events held at 16 different hospitals in Korea. Subjects were eligible to participate if they were 18 years of age or older, had a histologically confirmed diagnosis of breast cancer, had no evidence of recurrence or metastasis, and had no psychological problems at the time of the survey. Employment status, marital status, education, and income were assessed for patient socioeconomic status. Altered appearance distress was measured using the NCI's cancer treatment side effects scale; body image and quality of life were measured by the EORTC QLC-C30 and BR23. Means and standard deviations of each outcome were compared by socioeconomic status and multivariate linear regression models for evaluating the association between socioeconomic status and altered appearance distress, body image, and quality of life. Results: A total of 126 breast cancer patients participated in the study; the mean age of participants was 47.7 (SD=8.4). Of the total, 83.2% were married, 85.6% received more than high school education, 35.2% were employed, and 41% had more than $3000 in monthly household income. About 46% had mastectomy, and over 30% were receiving either chemotherapy or radiation therapy at the time of the survey. With fully adjusted models, the employed patients had significantly higher altered appearance distress (1.80 vs 1.48; p<0.05) and poorer body image (36.63 vs 51.69; p<0.05) compared to the patients who were unemployed. Higher education (10.58, standard error (SE)=7.63) and family income (12.88, SE=5.08) was positively associated with better body image after adjusting for age, disease stage at diagnosis, current treatment status, and breast surgery type. Similarly, patients who were married and who had higher education had better quality of life were statistically significant in the multivariate models. Conclusions: Socioeconomic status is significantly associated with altered appearance distress, body image, and quality of life in Korean women with breast cancer. Patients who suffer from altered appearance distress or lower body image are much more likely to experience psychosocial, physical, and functional problems than women who do not, therefore health care providers should be aware of the changes and distresses that these breast cancer patients go through and provide specific information and psychosocial support to socioeconomically more vulnerable patients.

새로운 항암성 백금(Ⅱ) 착체류의 in vivo 독성연구

정광원,임종석,정세영,노영수,최병기 한국환경독성학회 1995 환경독성보건학회지 Vol.10 No.2

The general toxicological study of new platinum(Ⅱ) compounds, KHPC-002, KHPC-005 and KHPC-006 were investigated in rats. The effects of these Pt(Ⅱ) complexes on renal, hematopoietic and hepatic system in rats showed lower toxicity compared with cisplatin. In the consideration of the maximal dose of these Pt(Ⅱ) complexes using in this experiment is 4-8 times higher than cisplatin, these novel compounds will have the less general toxicity in vivo.

저압 기체 모세관 방전의 전력 조건

정세민 東亞大學校附設基礎科學硏究所 2002 基礎科學硏究論文集 Vol.19 No.1

외경 8 mm, 내경 2 mm, 길이 80 cm인 파이렉스 유리 모세관으로 제작된 저압 기체 방전관의 방전조건들 중에서 전력 사용 조건을 방전관의 Ⅰ-Ⅴ 측정을 통해 조사해 보았다. 충진 기체는 He로써 1 Torr에서 10 Torr까지 변화되었다. 방전관의 방전 개시 전압(V_B)는 충진압(p)에 따라 증가되었으며, 방전관의 등가 저항(R_E)은 전류 제한용 저항값(R_B) 감소됨에 따라 감소되어 R_B→9 ㏀일 때 R_E→0이 됨을 알 수 있었다. 충진압(p)의 증가에 따른 V_B의 증가 때문에 전극 간격(d)을 감소시킨 결과, 주어진 10 kV, 0.1 A인 전원에 대해 p=1 Torr, d=1 cm 이고 R_B=10 ㏀일 때, V_B=3 kV였으며, 최대 동작 전류가 0.05 A로 주어짐을 알 수 있었다. d의 감소에 따른 p와 V_B의 한계는 조사되지 않았다. 실험 결과, R_B의 범위는 20 ㏀≥R_B≥10 ㏀, 용량은 270 W이상이 최적이었으며, 전원 역시 여러 충진압에서 동작점들을 고려해서 충분히 크게 정해야함을 알 수 있었다. 마지막으로 본 실험의 V_B들은 알려진 결과^1)보다 높게 나타났으며, 이는 모세관 방전의 특징으로 생각되었다.