신경독성 물질에 폭로되지 않은 건강한 남자의 신경행동학적 검사 수행능력

이세훈,김형아,이원철,장성실,이경재,박정일,정치경 大韓産業醫學會 1995 대한직업환경의학회지 Vol.7 No.1

Five items among neurobehavioral core test battery of World Health Organization, including Santa Ana dexterity, pursuit aiming, digit symbol, simple reaction time, and Benton visual retention, were administered to the workers occupationally non-exposed to neurotoxic agents by operational guide for the WHO neurobehavioral core test battery. Subjects were volunteers from the industrial workers(117) and clerks(40). Detailed occupational history such as exposed agents including noise or vibration, educational level, residence, smoking and drinking habit were recorded. Most of the neurobehavioral tests were correlated with age and educational level. Smoking was correlated with pursuit aiming and digit symbol. Vibration affected pursuit aiming. Job type(industrial workers vs. clerks) and noise exposure did not affected among tested items. Multiple regression analysis was done for establishing predict equation to estimate normal ranges of the tests for male workers. Age was shown to be main independent variable affected for all tests except wrong dot of pursuit aiming. Educational level was also shown to affected most of the tests except Santa ana dexterity and wrong dot of pursuit aiming. Alcohol consumption affected Santa Ana dexterity, non-preferred hand, and smoking affected only wrong dot of pursuit aiming after multiple regression analysis.

흰쥐 콩팥여과관문의 노화 변화에 관한 투과전자현미경적 연구

이세정,임형수,임도선,황덕호 韓國電子顯微鏡學會 2008 Applied microscopy Vol.38 No.2

콩팥의 모세여과관문은 토리모세혈관, 내피세포, 혈관사이바탕질, 토리바닥막, 발세포로 구성되어 있다. 콩팥의 노화가 일어나게 되면 형태학적 변형이 일어난다고 보고되었는데, 그 중 흰쥐의 경우 생쥐나 사람보다 노화에 따른 토리의 형태가 다양하게 관찰된다는 보고가 있다. 하지만 이런 연구들은 대부분 단면 위주로, 형태변화에 관한 입체적인 연구는 부족한 실정이다. 본 연구에서는 3개월이 지난 성숙한 흰쥐와 24개월이 지난 노화 흰쥐의 토리 모세여과관을 비교하여 그에 따른 형태변화를 광학 및 투과전자현미경을 이용하여 관찰하였으며, 이러한 결과를 바탕으로 연속절편을 통해 컴퓨터 프로그램을 기반으로 한 3차원 재구성을 시행하였다. 그 결과 노화 흰쥐의 토리에서 요공간의 확장, 모세혈관 내피세포의 변형, 토리바닥막의 비대, 혈관사이바탕질의 확장을 관찰할 수 있었다. 또한 3차원 재구성을 시행한 결과 노화 흰쥐의 토리에서 바닥막 경계의 붕괴 현상, 발세포 핵막의 조각화와 분절 양상, 발세포 세포돌기의 부분적인 수축과 세포돌기의 가늘어짐으로 인한 여과틈새의 확장을 관찰할 수 있었다. 이상의 결과로 미루어 볼 때, 노화가 진행되면 토리의 여과관문의 변형으로 인하여 콩팥의 생리학적 역할과 조절이 영향을 받을 것으로 사료된다. The filtration barrier of kidney consists of endothelial cell, glomerular capillary, glomerular basement membrane, mesangial matrix, and podocyte. In aged rats, the morphological changes were shown in various parts, including the glomerulus. These changes were thickening of basement membrane and mesangial matrix, crescent formation of glomerular capillary, deformity of foot processes, glomerular sclerosis and obsolescence. But these glomerular morphologies are partial images or few serial images analysis. In this study, we examined the morphological alteration of glomerulus in the young and aged rats by light microscopy, transmission electron microscopy and three dimensional reconstruction. We were found in aged rat glomerulus, expansion of urinary space and mesangial matrix, thickening and degrading of glomerular basement membrane, decreasing in podocyte foot processes, fragmentation of podocytic nucleus membrane. These observations indicate that may provide useful data for investigating the pathogenesis of age-related dysfunction of kidney.

李仁老의 古詩에 대한 一考察

조세형 서경대학교 인문과학연구소 1997 人文科學硏究 Vol.4 No.-

3차 의료기관에 입원한 노숙환자에서 감염질환의 유병율

함세운,남기석,노혜진,홍경득,황래석,방지환,신형식 대한감염학회 2007 감염과 화학요법 Vol.39 No.4

목적 : 3차 의료기관에서 입원치료를 받았던 노숙자를 대상으로 감염성 질환의 유병율을 알아보고자 하였다. 재료 및 방법 : 2002년 1월부터 2004년 8월 사이에 국립의료원에서 입원 치료를 받았던 269명의 노숙자 환자들을 대상으로 의무기록을 조사하였다. 결과 : 연구 기간동안 입원치료를 받은 총 269명의 노숙자 환자 중 남자가 249명(92.9%), 여자가 19명(7.1%)이었다. 감염질환의 유병율은 B형 간염이 228명 중 15명(6.6%), C형 간염 113명 중 8명(7.1%), 결핵은 269명중 19명(7.1%), VDRL 양성인 경우는 234명 중 25명(10.7%), 항사람면역결핍바이러스항체 양성은 130명중 2명(1.5%)이었다. B형 간염표면항원에 대한 항체 형성자는 118명(51.7%), 항체 미형성자는 95명(41.7%)이었다. 결핵으로 진단된 19명 중 객담도말 및 배양검사와 조직검사로 확인된 경우가 14명, 균음성이나 임상적으로 의심되는 경우가 5명이었다. 간질환 및 관련된 합병증으로 입원한 환자 28명 중 B형 및 C형 간염바이러스에 관련된 경우는 5명(17.9%)에불과하였고 결핵은 19명중 13명(68.4%)이 폐결핵 및 림프절종대의 증상으로 입원치료를 받았다. 복합 감염의 경우는 총 9명이었는데 후천면역결핍증후군 환자였던 2명중 1명이 결핵에 이환되어 있으면서 VDRL 양성소견을 보였고 다른 1명이 HIV와 결핵의 동시감염이었다. 결론 : 노숙자 입원환자에서 결핵, C형 간염 및 매독의 유병율이 일반 인구에 비해 높았다. 노숙자환자를 담당하는 의료인력를 보호하고 환자의 감염질환 유무를 파악하기 위해 적절한 선별검사가 필요하며 감염질환의 확산을 방지하기 위한 정부의 적극적인 대책이 필요하다. Background : Despite the expected high prevalence of several infectious diseases among the homeless, a majority of them have not received screening test and early treatment. Materials and Methods : The subjects of this study were 269 homeless patients who had been hospitalized in the National Medical Center (Korea) between January 2002 and August 2004. Their medical histories and records were reviewed to analyze the prevalence and clinical characteristics of hepatitis B and C, syphilis, tuberculosis and HIV infection among them. Results : The entire 269 homeless patients being hospitalized during the period of this study consist of 249 male patients and 19 female patients. For the prevalence of infectious diseases, HBV was seen in 15 of 228 patients (6.6%); HCV in 8 of 113 patients (7.1%); VDRL positive in 25 of 234 patients (10.7 %); HIV antibody positive in 2 of 130 (1.5%); and tuberculosis in 19 of 269 (7.1%). The number of patients who had the antibody to hepatitis B was 118 (51.7%). Of 28 patients hospitalized with the chief complaints of liver diseases and/or their complications, the number of cases infected with hepatitis B and C viruses was only 5 (17.9%). On the other hand, for patients with tuberculosis, 13 of 19 cases (68.4%) were hospitalized with the chief complaints associated with symptoms of pulmonary tuberculosis or enlarged lymph nodes. The number of patients with multiple infectious diseases was 9 in total. Conclusion : As expected, the prevalence of some infectious diseases were higher among the homeless inpatients, than non-homeless population. Proper screening tests in order to determine the presence of any infectious diseases among the patients and protect involved medical staff are necessary. Further, the government should take proactive measures to prevent the spread of infectious diseases in the aspect of public health.

可變構造시스템(VSS)을 이용한 直流 서어보 驅動裝置 制御에 관한 硏究

李炯基,權世鉉,金錫哲 釜山工業大學校 1990 論文集 Vol.32 No.-

A control system of dc machine position, speed or current control based on variable structure system approach is presented. The deliberate introduction of sliding mode leads to good dynamic and steady state behavior of the overall system. Amethod of the switching function estimation based on control error and armature current information is proposed. In addition the external torque observer is constructed. The simulation of the proposed control result shows that the system motion is as it was predictioned from theoretical analysis.

Stem cell과 Myeloperoxidase가 스티렌, 하이드로퀴논 및 트리클로로에틸렌에 의한 림프구의 자매염색분체 교환과 소핵체 유도에 미치는 영향

이경재,김형아,신민정,성재혁,박정일,한훈,이세훈 대한산업의학회 2001 대한직업환경의학회지 Vol.13 No.3

목적 : 스티렌, 하이드필퀴논 및 트리클로로에틸렌 (TCE)이 사람의 stem cell과 human myeloperoxidase (MPO)에 의해 대사성 활성화되는 지를 규명하고자 스티렌, 하이드로퀴논 및 TCE에 사람의 stem cell 또는 MPO 효소의 첨가가 이들 화학물질에 의한 자매염색분체교환(SCE)과 소핵체 (MN) 빈도에 미치는 영향을 관찰하였다. 방법 : 건강한 남자의 전혈에서 림프루를 분리하여 72시간동안 이중배양하되 배양개시 24시간만에 0.05mM 하이드로퀴논, 1.50 mM 스티렌, 혹은 1.50mM TCE를 전체용량이 30 ㎕가 되도록 아세톤에 희석하여 배양액에 주입하였고 대조군은 아세톤으로 처리하였다. 화학물질 처리 후 즉시 1.3×106 및 2.6×106 cells/ml 농도의 제대혈액으로부터 나온 stem cell 세포액의 상층액이나 1.0 및 2.0 unit의 human myeloperoxidase를 H2O2와 함께 첨가하였다. SCE분석을 위한 배양액에는 배양종료 2.5시간 전에 colchicine을 가한 후 수확하여 Giemsa염색을 하여 metaphase 세포에서 SCE빈도를 분석하였다. MN분석을 위한 배양액에는 배양개시 44시간만에 cytochalasin-B를 가하였고 acridine orange 염색 후 이핵체에서 MN수를 분석하였다. 결과 : 1. stem cell이나 MPO 자체는 SCE나 MN의 빈도에 영향을 미치지 않았다. 2. stem cell이나 MPO는 스티렌에 의해 유도되는 SCE의 빈도를 용량-반응관계로 유의하게 증가시켰고, MN빈도의 경우 step cell이나 MPO에 의해 증가되는 경향이 있었으나 2.0 unit MPO를 첨가한 경우에만 첨가하지 않은 경우에 비하여 유의하게 증가하였다. 3. 하이드로퀴논은 stem cell이나 MPO가 없는 상태에서도 대조군에 비하여 SCE빈도가 대조군에 비하여 높았다. stem cell이나 MPO는 하이드로퀴논에 의한 SCE 빈도를 용량 반응관계로 유의하게 증가시켰지만, MN의 경우에는 증가시키는 경향만 있을 뿐 유의한 차이는 아니었다. 4. TCE자체는 SCE나 MN빈도를 증가시키지 않았다. stem cell은 1.3 ×106 및 2.6 × 106 cells/ml 농도 모두에서 SCE빈도를 유의하게 증가시켰고 MPO는 2.0 unit농도에서만 유의하게 증가시켰다. stem cell이나 MPO모두 TCE에 의한 MN빈도를 증가시키는 경향이 있었으나 유의한 차이는 아니었다. 결론 : 저자들은 스티렌, 하이드로퀴논, 및 트리클로로에틸렌에 의해 유도되는 자매염색분체교환과 소핵체의 빈도가 사람의 stem cell이나 myeloperoxidase에 의해 증가됨을 발견하였으며, 이러한 결과는 myeloperoxidase가 이들 물질의 대사성활성화에 관여함을 암시하고, 또한 아마도 이 물질들의 골수독성과 관련이 있는 것이라고 제시된다. Objectives : The objective of this study was to identify the possible role of stem cell and myeloperoxidase (MPO) in the metabolic activation of styrene, hydroquinone and trichloroethylene, by investigating the effects of stem cell from umbilical cord blood and MPO on the frequency of sister chromatid exchange (SCE) and micronuclei (MN) induction in cultured human peripheral lymphocytes exposed to hese chemicals. Methods : Islated lymphocytes from whole blood were cultured for 72 hours. The cells were treated with 1.50 mM styrene, 50 μM hydroquinone and 1.50 mM trichloroethylene dissolved with acetone (30㎕ in total volume) at 24 hours after the beginning of culture. Control group was treated with acetone only. Immediately after adding these chemicals, 1.3×106 cells/ml and 2.6×106 cells/ml stem cell/ml stained with Giemsa's solution, and acridine orange for sister chromatid exchange, and for micronucleus analysis, respectively. Results : The results were as follows: 1) Myeloperoxidase and stem cell did not significantly affect the frequencies of SCE or MN in the control group. 2) The frequency of SCE or MN with exposure to styrene did not different from control in the absence of stem cell or MPO. Sister chromatid exchange induced by styrene was significantly increased by adding stem cell or MPO in dose-dependent relationship. The frequency of MN induced by styrene significantly increased in the presence of 2.0 unit MPO. 3) The frequency of SCE was significantly increased with exposure to hydroquinone than acetone treated control in the absence of stem cell or MPO. Sister chromatid exchange induction by hydroquinone significantly increased dose-dependently in the presence of stem cell or MPO. There was a tnendency of increase of the MN frequency induced by hydroquinone in the presence of stem cell or MPO, but not significant. 4) It was found that trichloroethylene itself did not increase SCE or MN frequency. Frequency of SCE induced by trichloroethylene was significantly increased with adding stem cell (low and high) and 2.0 unit MPO. Even though them cell or MPO increased the frequency of MN of lymphocyte exposed to trichloroethylene, the difference was not significant. Conclusions : Authors found that the frequencies of both sister chromatid exchange and micronucleus induced by styrene, hydroquinone, and trichloroethylene were increased significantly with the treatment of stem cell or myeloperoxidase. It was suggested that myeloperoxidase may therefore play an important role in the metabolic activation of styrene, hydroquinone, and trichloroethylene and myeloperoxidase probably be involved in the myelotoxicity of these chemicals.

가변구조 제어시스템의 Luenberger Observer에 관한 연구

탁한호,이형기,박양수,권세현 釜山工業大學校 1991 論文集 Vol.33 No.-

This paper presents an analysis of the stste observer-based robust speed control of a self-controlled synchonous motor (SCSM). A Variable structure control (VSC) technique is utilized to achieve robust (paramater-insensitive) characteristics. The speed and acceleration signals required for the implementation of the variable structure speed control (VSSC) are dynamically estimated with stste observer. One kind observer-the Luenberger full order observer-air explored in this paper. The results obtained that Luenverger observers do estimate the system ststes accurately when the system parameters vary. The design methods and the simulation results presented demonstrable the potential of the proposed scheme.

적혈구 유무에 따른 벤젠, 스티렌, 트리클로로에틸렌에 의한 림프구 중 자매염색분체 교환 빈도

성재혁,김형아,신민정,최미정,이세훈 가톨릭대학교 산업의학센터 2001 韓國의 産業醫學 Vol.40 No.4

This study was performed to investigate the possible role of oxyhemoglobin in the metabolic activation of benzene, styrene and TCE by observing the difference of the SCE frequency between isolated lymphocyte and whole blood lymphocyte cultures exposed to these chemicals. Isolated lymphocyte and fresh heparinized peripheral whole blood samples from a healthy donor(male, smoker) were cultured in the phytohaemagglutinin stimulated culture media for 72 hours. The cells were treated with 100, 300, 1000, 3000 μM benzene, 250, 500, 100, 2000 μM styrene or TCE respectively. Slides were stained with Giemsa's solution for SCE. SCE was analyzed for each subject from coded slides by one researcher. The results were as follows : 1.The frequency of SCE increased dose-dependently with concentration of benzene in isolated lymphocyte and whole blood lymphocyte cultures, however there were no significance. There was no significant difference of SCE frequency between isolated lymphocyte and whole blood lymphocyte cultures. 2.The frequency of SCE in whole blood exposed to sytrene significantly increased in dose-dependant relationship, but not in isolated lymphocyte. The difference of SCE frequency between isolated lymphocyte and whole blood lymphocyte of 2000μM styrene exposure was marginally significant(P=0.0540). 3.In TCE, the frequency of SCE did not increase at any concentration used in this study of isolated lymphocyte and whole blood lymphocyte. Above results suggested that oxyhemoglobin in erythrocytes play an important role in the metabolic activation of styrene but not in benzene or TCE. Key Words : Benzene, Styrene, Trichloroethylene, Sister chromatid exchange, Metabolic activation, Oxyhemoglobin

혈관내초음파로 평가한 스텐트를 삽입한관동맥의 형태학적 특징

정남식,홍범기,임세중,백성일,이문형,장양수,심원흠,조승연,김성순 대한순환기학회 1997 Korean Circulation Journal Vol.27 No.8

산업보건관리자를 위한 소음성 난청 예방지침서의 개발

이원철,유경혜,장성실,이세훈,구정완,김현욱,노영만,이경재,김형아 가톨릭 대학 산업의학 쎈타 산업의학연구소 1996 韓國의 産業醫學 Vol.35 No.1

Occupational hearing loss became the leading occupational disease by constioution 56% of all work-related diseases in Korea. However, the prevalance rates showed wide variations among the specific medical examination providers and were very low(0.8-1.4%) compared with those 8.4% in Japan. The sources of these variations was regarded in the preview study as 1) Employee audometric estings were primarily conducted by nurses and nurse-aids who had no formal training for audiometry; 2) althought audiometric testing equipment used by the spedific medical examination providers, it should be periodically calibrated, a significant number of them were not properly calibrated : 3) Initial audiometric testings were conducted at any available spaces in workplace or at the testing room without considering bachground noise level. One of solutions that can be done for sloving surrent noise related problems is to provide a noise related manual for the occupational health managers including doctors, nurses and hyginests. This study was done to provide such a manual. The main core of the manual was hearing conservation program. And the contents of the manual was the same as the main frame of hearing conservation program such as noise survey, provided of the diagram showing the core contents of each factor of the hearing conservation program. Hopefully, this manual will be the references of the standardized performances of the specific medical examination providers.