http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : Saroj (검색결과 76 건)
- 무료
- 기관 내 무료
- 유료
-
-
-
Saroj Kumar Sahoo,Mamata Mohapatra,Shashi Anand 한국물리학회 2013 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.62 No.2
A series of nano ceria samples containing 0 to 2 mol% europium were synthesized following the coprecipitation hydrothermal technique. The X-ray diffraction patterns showed peaks corresponding to the fluorite structure of cubic ceria with shifting of the peak positions, but did not show any Eu-related peaks. The fourier transform infrared (FTIR) spectra of all the samples showed four bands that could be attributed to Ce-O stretching vibrations, formation of nano-crystalline particles, −HOH bending, and −OOH and −OH stretching vibrations. The Raman spectra of all the samples showed a sharp and intense Raman shift at around 460 - 462 cm<sup>−1</sup> corresponding to the triplydegenerate F<sub>2g</sub> mode. The intensity of the Raman peaks showed variations in intensities. The transmission electron microscope (TEM) images of typical samples revealed that the shapes and the sizes of the particles were not much affected after Eu doping in the studied range though Eu doping resulted in agglomerations of nanoparticles. Broad absorption peaks centered at 292, 332, 336, 310, and 294 nm were observed for 0, 0.25, 0.5, 1, and 2 mol % Eu-doped ceria in the UVVis spectra with direct band gaps of 3.6, 2.75, 2.75, 2.75, and 3.25 eV, respectively.The smaller values of the indirect band gap indicated that Eu-doped nanoparticles were available to assist the indirect electron transition from the valence band to the conduction band. The emission peaks for the spectra obtained by exciting at 300 nm were observed at 355, 405, 396, 387, and 380 nm for pure ceria sample and 0.25, 0.5, 1, and 2 mol% Eu-doped samples, confirming the red shift in all the doped samples. The spectra for Eu-doped samples obtained by exciting at 500 nm showed three peaks in the orange-red region corresponding to the 5D<sub>0</sub> ∪ 7F<sub>j</sub> (j = 0 - 4) transitions of Eu<sup>3+</sup>.
-
( Saroj Nepal ),( Pil Hoon Park ) 영남대학교 약품개발연구소 2015 영남대학교 약품개발연구소 연구업적집 Vol.25 No.-
Adiponectin,an adipokine predominantly secreted from adipose tissue, exhibits diverse biological responses,including metabo-lism of glucose and lipid, and apoptosis in cancer cells. Recently, adiponectin has been shown to modulate autophagy of cancer cells,the role of autophagy in apoptosis of cancer cell caused by adiponetin has not been explored. In the present study,we dem-onstrated that globular adiponectin(gAcrp)increase in caspae-3 activity,Bax,microtubule-associated protein light chain 3-ll(L C3 ll)protein levels,and dutoptposis in both HepG2 and MCF-7 cell lines,whereas induction of autophagy by rapamycin, an mTORinhibitor,significantly prevented gAcrp-induced Bax expression in HepG cells.These results implicate that induction of autophagy plays a regulatory role in adiponectin-induced apoptosis of cancer cells,and thus inhibition of autophagy would be a novel promising taiget to enhance the efficiercy of cancer cell apoptosis by adiponectin.
-
( Saroj Nepal ),( Mi Jin Kim ),( Eung Seok Lee ),( Jung Ae Kim ),( Dong Yong Choi ),( Dong Hwan Sohn ),( Sung Hee Lee ),( Kyung Song ),( Sang Hyun Kim ),( Gil Saeng Jeong ),( Tae Cheon Jeong ),( Pil H 영남대학교 약품개발연구소 2014 영남대학교 약품개발연구소 연구업적집 Vol.24 No.0
Globular adiponectin (gAcrp) protects liver cells from ethanol-induced apoptosis via induction of autophagy. However, the underlying mechanisms are unknown. The present study aims to investigate the potential role of autophagy-related protein 5 (Atg5), an essential Atg for the elongation of autophagosomes, in suppression of ethanol-induced cytotoxicity by gAcrp. Here, we demonstrated that suppression of Atg5 expression by ethanol was restored by pretreatment with gAcrp both in primary rat hepatocytes and human hepatoma cell line (HepG2). Moreover, ethanol-induced accumulation of p62 (sequestosome1), a marker of autophagic flux, was restored by gAcrp treatment, implying that gAcrp modulates autophagic flux in liver cells. Further, Atg5 silencing prevented p62 degradation by gAcrp, suggesting that Atg5 plays a critical role in induction of autophagic flux by gAcrp. Interestingly, gene silencing of Atg5 by siRNA abrogated restoration of autophagosome formation by gAcrp in ethanol-treated cells. Finally, protection of liver cells by gAcrp from ethanol-induced apoptosis was also significantly attenuated by knocking-down of Atg5 expression, suggesting an important role of Atg5 in autophagy induction and cellular apoptosis modulated by gAcrp. Taken together, our data demonstrated that Atg5 expression, at least in part, is implicated in gAcrp-induced autophagy and subsequent anti-apoptotic effects in ethanol-treated liver cells.ⓒ2014Elsevier Ltd. All rights reserved
-
( Saroj Nepal ),( Pil Hoon Park ) 영남대학교 약품개발연구소 2014 영남대학교 약품개발연구소 연구업적집 Vol.24 No.0
Hepatocellular apoptosis is important pathological entity of alcoholic liver disease. Previously, we have shown that globular adiponectin (gAcrp) protects liver cells from ethanol-induced apoptosis by modulating an array of signaling pathways. In the present study, we investigated the role of autophagy induction by gAcrp in the suppression of ethanol-induced apoptosis and its potential mechanism(s) in liver cells. Here, we demonstrated that gAcrp significantly restores ethanol-induced suppression of autophagy-related genes,including Beclin-1 and microtuble-associated protein light chain (LC3B) both in primary rat hepato-cytes and human hepatoma cell line (HepG2). Globular adiponectin also restored autophagosome formation suppressed by ethanol treatment in HepG2. Furthermore, inhibitionof gAcrp-induced autophagic process by knock-down of LC3B prevented protection from ethanol-induced apoptosis. In particlar, the autophagic process induced by gAcrp was involved in the suppression of ethanol-induced activation of caspase-8 and expression of Bax. Moreover, knock-down of AMPK by small interfering RNA (siRAN) blocked gAcrp-induced expression of genes related to autophagy, which in turn prevented protection from ethanol-induced apoptosis, suggesting that AMPK plays an important role in the induction of autophagy and protection of liver cells by gAcrp. Finally, we also showed that gAcrp treatment induces translocation of the forkhead box O member protein FoxO3A, into the nucleus, which may play a role in the induction of autophagy-related genes. Taken together, our data demonstrated that gAcrp protects liver cells from ethanol-induced apoptosis via induction of autophagy. Further, the AMPK-FOXO3A axis plays a cardinal role in gAcrp-induced autophagy and subsequent inhibition of ethanol-induced apoptosis.
-
( Saroj Nepal ),( Mi Jin Kim ),( Amit Subedi ),( Eung Seok Lee ),( Chul Soon Yong ),( Jung Ae Kim ),( Wonku Kang ),( Mi Kyung Kwak ),( Dharamvie Singh Arya ),( Pil Hoon Park ) 영남대학교 약품개발연구소 2013 영남대학교 약품개발연구소 연구업적집 Vol.23 No.0
Hepatocellular apoptosis is an essential pathological feature of alcoholic liver disease. Adiponectin, an adipokine predominantly secreted from adipose tissue, has been shown to play beneficial roles in alcoholic liver disease against various inflammatory and pro-apoptotic molecules. However, the effects of adiponectin on ethanol-induced apoptosis in liver cells are largely unknown. Herein, we investigated the role of globularadiponectin (gAcrp) in the prevention of ethanol-induced apoptosis and further tried to decipher the potential mechanisms involved. In the present study, we demonstrated that gAcrp significantly inhibits both ethanol-induced increase in Fas ligand expression and activation of caspase-3 in human hepatoma cell lines (HepG2 cells), suggesting that gAcrp plays a protective role against ethanol-induced apoptosis in liver cells. This protective effect of gAcrp was mediated through adiponectin receptor R1 (adipoR1). Further, globular adiponectin treatment caused induction of heme oxygenase-1 (HO-1) through, at least in part, nuclear factor (erythroid-derived 2)-like 2, (Nrf2) signaling. Treatment with SnPP, a pharmacological inhibitor of HO-1, and knockdown of HO-1 with small interfering RNA (siRNA) restored caspase-3 activity suppressed by gAcrp, indicating a critical role of HO-1 in mediating the protective role of gAcrp in ethanol-induced apoptosis in liver cells. In addition, carbon monoxide, a byproduct obtained from the catabolism of free heme was found to contribute to the anti-apoptotic effect of adiponectin. In conclusion, these data demonstrated that globular adiponectin prevents ethanol-induced apoptosis in HepG2 cells via HO-1 induction and revealed a novel biological response of globular adiponectin in the protection of liver injury from alcohol consumption. ⓒ2012 Elsevier lnc. All rights reserved.
-
Saroj Mishra,Benu Sethi,Monika Jain,Manish Chowdhary,Yogesh Soni,Yukti Bhatia,Vikram Sahai 한국생물공학회 2002 Biotechnology and Bioprocess Engineering Vol.7 No.1
The cloning and expression of β-glucosidase II, encoded by the gene bglu2, from thermo- tolerant yeast Pichia etchellsii into Escherichia coli is described. Cloning of the 7.3 kb BamHI/SalI yeast insert containing bglu2 in pUC18, which allowed for reverse orientation of the insert, resulted in better enzyme expression. Transformation of this plasmid into E. coli JM109 resulted in accumulation of the enzyme in periplasmic space. At 50oC, the highest hydrolytic activity of 1686 IU/g protein was obtained on sophorose. Batch and fed-batch techniques were employed for enzyme production in a 14 L bioreactor. Exponential feeding rates were determined from mass balance equations and these were employed to control specific growth rate and in turn maximize cell growth and enzyme production. Media optimization coupled with this strategy resulted in increased enzyme units of 1.2 kU/L at a stabilized growth rate of 0.14 h-1. Increased enzyme production in bioreactor was accompanied by formation of inclusion bodies.
-
Saroj Prasad Deo,Md Shakeel Ahmad,Abanish Singh 대한구강악안면외과학회 2022 대한구강악안면외과학회지 Vol.48 No.1
Objectives: Inferior alveolar nerve block (IANB) is commonly used for mandibular dentoalveolar surgery. The objective of this study was to evalu-ate and compare the effectiveness of coadministration of dexamethasone (4 mg/mL) or adrenaline (0.01 mg/mL) as an adjuvant with lignocaine 2% in IANB during third molar surgery (TMS). Patients and Methods: This double-blind, randomized controlled trial was conducted between March and August 2020. The investigators screened patients needing elective TMS under local anesthesia. Based on strict inclusion and exclusion criteria, patients were enrolled in this study. These pa-tients were assigned randomly into two study groups: dexamethasone group (DXN) or adrenaline group (ADN). Outcome variables were postoperative edema, trismus, visual analogue scale (VAS), perioperative analgesia, onset time, and duration of IANB. Results: Eighty-three patients were enrolled in this study, of whom 23 (27.7%) were eliminated or excluded during follow-up. This study thus included data from 60 samples. Mean age was 32.28±11.74 years, including 28 females (46.7%) in the ADN (16 patients, 57.1%) and DXN (12 patients, 42.9%) groups. The duration of action for DXN (mean±standard deviation [SD], 4:02:07±0:34:01 hours; standard error [SE], 0:06:00 hours; log-rank P=0.001) and for ADN (mean±SD, 1:58:34±0:24:52 hours; SE, 0:04:42 hours; log-rank P=0.001) were found. Similarly, time at which 1st analgesic consume and total number of nonsteroidal antiinflammatory drugs need to rescue postoperative analgesia was found statistically significant between study groups (t (58)=–11.95; confidence interval, –2:25:41 to –1:43:53; P=0.001). Early-hours VAS was also significantly different between the study groups. Conclusion: A single injection of dexamethasone prolongs the duration of action of lignocaine 2% IANB. Additionally, it can be used in cases where adrenaline is contraindicated.
-
Global Variations in Curriculum and Syllabus for Endocrine Surgery Training Programs: a Report
Saroj Kanta Mishra 대한갑상선-내분비외과학회 2019 The Koreran journal of Endocrine Surgery Vol.19 No.1
Purpose: The aim of this study is to find out information relevant to the certification of trainees in surgical endocrinology specialty—duration of training, entry/exit criteria, curriculum and accreditation agency. Methods: Information related to endocrine surgery training programs was archived from electronic resources such as publications, web portals of official endocrine surgery professional bodies and institutions offering such courses and the data was analysed. Results: The 75 trainee positions were identified globally in 58 institutions—28 in North America, 5 in Latin America, 17 in Australia and New Zealand, 10 in Asia, 25 in Europe and none in Africa. The average length of training leading to award of fellowship/diploma/degree ranged from 1 to 3 years. The curriculum of majority (n=58) of these programs includes thyroid, parathyroid, adrenal and neuroendocrine tumors although in many centres it also includes breast (n=25, 43%), diabetic foot (n=12, 21%), thymus (n=17, 30%) and salivary gland (n=4, 7%) thus suggesting variation worldwide. The assessment of trainees at the end of course also differs. Conclusion: There are no uniform guidelines across the world for endocrine surgery training programs unlike those developed for other sub-specialities of surgery.
ScienceON연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
