CD34+ 조혈 모세포 이식 2례

김정아,정현식,김원석,윤성수,이홍기,박찬형,박성규,김동욱,이종욱,한치화,민우성,김춘추,김동집 대한조혈모세포이식학회 1996 대한조혈모세포이식학회지 Vol.1 No.1

Background: In most solid tumors, the CD34 antigen has not been detected, so positive selection of CD34+ cells may reduce tumor cell contamination and the CD34+ cells are capable of reconstituting hematopoiesis. We tried CD34+ cell transplantation in two patients. Method: CD34+ cells from chemotherapy + G-CSF mobilized PBPCs or bone marrow were positively selected with an avidin-biotin immunoadsorption column (CEPRATE SC system). Case 1. One course of chemotherapy using cyclophosphamide(200㎎/㎡) and etoposide (4.2g/㎡), combined with G-CSF(5㎍/㎏) S.C. was used in a relapsed lymphoma patient. This patient responded to the induction chemotherapy. CD34+ cells from harvested bone marrow were selected by the CellPro immunoadsorption column. The total number of mononuclear cells loaded onto the CellPro was 2.4×10^(8)/㎏, with 1.1% CD34+ cells. After column separation, the total number of positively selected cells was 5.16×10^(6)/㎏. The number of CFU-GM was 76.8×10⁴/㎏. This patient was treated with melphalan (140㎎/㎡) and TBI (1200cGy) and the positively selected CD34+ cells were infused. The time to neutrophil recovery greater than 0.5×10^(9)/L was 19 days and the time to platelet recovery greater than 50×10^(9)/L was 21 days. Case 2. Two courses of mobilizing chemotherapy were given 4 weeks apart using taxol(210㎎/m2) and adriamycin(60㎎/m2), combined with G-CSF(5㎍/㎏) S.C. in a breast cancer patients with 7 axillary node metastasis. CD34+ cells from each single leukapheresis product were selected by the CellPro immunoadsorption column. In the first collection, the total number of nucleated cells was 4.4×10^(8)/kg, with 0.42% CD34+ cells. In the second collection, the total number of nucleated cell was 2.8× 10^(8)/㎏ with 0.43% CD34+ cells. After colum separation, the total numbers of collected cells were 4.0×106/kg and 4.8×10^(6)/kg, the total number of CD34+ cells were 1.2×10^(6)/㎏ and 0.82×10^(6)/㎏. Colonogenic assays of positively selected CD34+ cells gave rise to myeloid erythroid, and multilineage colonies, with a median of 190 CFU-GM, 190 BFU-E, and 164 CFU-GEMM per 1×10³ adsorbed cells, respectively. High-dose chemotherapy with cumulative doses of 40mg/㎡ mitoxantrone, 750mg/㎡ thioptepa, and 1000mg/㎡ carboplatin was administered. Positively selected CD34+ cells were rapidly infused 24 hours after the end of high-dose chemotherapy. The time to neutrophil recovery greater than 0.5×10^(9)/L was 16 days and the time to platelet recovery greater than 50×10^(9)/L was 20 days.

축산 폐수의 효율적 처리를 위한 광합성 미세조류인 Spirulina platensis 배양 공정의 최적화

안주희,김성수,김태호,이준엽,오상집,이진하,이현용 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.4

돈분 폐수의 효율적인 처리를 위해 6 W/㎡, 12 W/㎡와 24 W/㎡의 조도 하에서 온도를 15℃에서 40℃까지 각각 변화시켜, 광합성 미세 조류인 Spirulina platensis의 생육에 관한 최적 생육 조건 결정에 관한 실험을 하였다. 균체의 비생육속도는 30℃까지는 온도 증가에 따라 증가하였다. 균체의 생육에 있어서 온도의 영향을 설명하기 위해서 Arrhenius 식을 이용하여서 얻은 활성화 에너지 값은 13.5kal/mol이었다. 순수 배지에서 배양된 균체의 비생육속도가 0.31(1/day)인 반면 30% 돈분 첨가 배양시 얻은 균체의 비생육속도는 0.24(1/day)였다. 폐수내의 총 질소량과 이의 제거속도로 균체의 폐수 처리 능력을 평가할 수 있었다. 돈분 농도를 달리 첨가한 회분배양에 있어서 70~93%(PO_4^3- -P), 67~93%(inorganic nitrogen), 80~90% (COD) 그리고 37~56%(organic nitrogen)의 감소율을 보였다. 총-질소와 총-인의 1차반응 감소 속도 상수는 각각 0.17(1/day)와 0.14(1/day)로 계산되었다. 20% 돈분을 첨가하는 연속배양에 있어서 회석률이 0.20(1/day)일 때 최대 균체농도가 1.52(g/L)를 유지했다. 돈분처리 후 얻은 균체의 성분은 단백질이 58.7%, 지방 11.0% 그리고 회분이 15.6%였다. The kinetics of growing microalga, Spirulina platensis was investigated to treat swine wastes with optimum growth conditions. Temperature was varied from 15 to 40℃ at three different light intensities, 6 W/㎡, 12 W/㎡ and 24 W/㎡. The specific growth rate was increased as temperature increased up to 30℃. The activation energy was estimated as 13.5 kcal/mol by an Arrhenius relationship. 0.24(1/day) of specific growth rate was obtained from batch cultivation with 30% swine wastes, compared to 0.31 (1/day) from clean culture. It was found that Spirulina platensis was able to reduce 70~93% of PO_4^3- -P, 67~93% of inorganic nitrogen, 80~90% of COD and 37~56% organic nitrogen by adding various concentrations of swine wastes for 12 days of batch cultivation. Rate constants for removing nitrates and phosphates in treating swine wastes were estimated as 0.17 (1/day) and 0.14 (1/day) in the first order reaction, respectively. 1.52(g/L) of maximum cell density was maintained at 0.20(1/day) of dilution rate in continuous culture, adding 20% swine wastes for 30 days. The chemical composition of the biomass obtained from the process showed 58.7% of protein, 11.0% of lipid and 15.6% of ash.

7個 結晶系의 唯一 X-線 回折强度 資料

徐日煥,金光柱,秋錦洪,李珍昊,林星秀,柳保盈,金文執 충남대학교 자연과학연구소 1992 忠南科學硏究誌 Vol.19 No.1

한 結晶의 構造는 唯一單位의 X-線 回折强度資料로부터 決定될 수 있다. Miller指數(hkl)의 函數인 回折强度Ⅰ(hkl)는 異常 散亂(anomalous scattering)이 無視될때 對稱中心點群 卽 Laue群의 對稱性을 나타낸다. 이 論文에서는 모든 結晶系에 對한 回折强度의 唯一單位를 Laue群들의 對稱性으로부터 誘導하여 놓았다. Crystal structures can be determined from the unique data of X-ray intensity. As the X-ray intensity Ⅰ(hkl), which is a function of diffraction plane (hkl), has a center of symmetry assuming anomalous scattering is negligible Ⅰ(hkl) shows the symmetry of a centrosymmetric point group, i.e., a Laue group. In this paper the unique data of the intensity for all crystal systems have been derived from the symmetries of Laue groups.

[ Pt ( 2 ) ( trans / - DACH ) ( PTP ) ] 2NO3 ( PC - 1 ) , 2NO3 / A Novel Class of Platinum Complex Exhibiting Selective Cytotoxicity to Various Cancer Cell - Linesand Normal Kidney Cells

(Sung Goo Chang),(Jee Chang Jung),(Young Soo Rho),(Kyung Tae Lee),(Jong Jip Park) 경희대학교 동서약학연구소 1997 연구업적집 Vol.12 No.-

Loyalty Behaviors in Cross-Cultural Environment: In the Case of Banking

( Sung Jip Nam ) 한국유통경영학회(구 한국유통정보학회) 2011 유통정보학회지 Vol.14 No.3

본 논문은 서양과 동양 소비자들의 고객충성도 관계에 관해 이문화적 관점으로 비교 분석 하였다. 고객 충성도에 관한 연구는 기업의 수익성과 직결된다는 중요성 때문에 현재 활발히 진행되어왔으나, 대부분의 연구는 한 국가의 테두리 내에서 수행되었다. 국제경영이나 이문화 관련 선행연구들에 따르면 한쪽 문화에서 밝혀진 연구 사실은 문화적 효과로 인하여 다른 문화에서는 통하지 않을 수도 있다고 한다. 또한 동양과 서양의 문화적 차이는 특히나 크다고 알려져 있다. 기업들의 활동이 글로벌화 됨에 따라 이문화 환경에서의 소비자들의 행동에 관한 필요성은 점차 커지고 있으나, 그러한 환경을 포함한 연구는 제한적이다. 본 논문에서는 동양과 서양의 표본국가로서 한국 소비자 그룹과 미국 소비자 그룹을 선택하였고 그들의 구전효과와 재구매 행태에 관하여 비교 분석 하였다. 또한 그들의 애호도 행동의 원인을 이문화적 관점에서 고찰하고자 한다. 양국 소비자들의 행태 비교의 동기는 이문화에 관한 선행연구에 그 기초를 두고 있다. This article compares the Western and the Eastern individual`s loyalty engagement activity in financial services from a cross-cultural perspective. Customer Loyalty has earned significant attention from both academia and practitioners due to its attachment to a firm`s profitability. However, most of research has performed in one geographical region. International Business and cultural literature suggest that scholarly findings from one culture may not be valid in other cultures due to cultural effects. The cultural differences between the Eastern and the Western worlds are especially pronounced. As corporations expands their territory to other continents, necessity of reflecting multi-cultural individual`s loyalty behavior is raised as well. American and Korean consumer groups are chosen to represent both cultures.

( 97 , October ) : Selective Cytotoxicities of New Pt ( 2 ) Complexes on Cancer Cell - lines and Normal Kidney Cells

(Sung Goo Chang),(Jee Chang Jung),(Young Soo Rho),(Kyung Tae Lee),(Jong Jip Park) 경희대학교 동서약학연구소 1997 연구업적집 Vol.12 No.-

ON A GRONWALL-TYPE INEQUALITY ON TIME SCALES

Sung Kyu Choi,Nam jip Koo 충청수학회 2010 충청수학회지 Vol.23 No.1

In this paper we extend a di??erential inequality pre-sented in Theorem 2.2 [6] to a dynamic inequality on time scales.

Chromium Methionine Supplementation Decreases Obesity Indices in Rats

Sang Jip Ohh,Chang Hyeuk Kim,Jong Seo Shin,Kyung Il Sung,Hyun Sook Kim 한국식품영양과학회 2003 Preventive Nutrition and Food Science Vol.8 No.3

This study was conducted to determine the effects of chromium-methionine (CrMet) supplementation at various levels on obesity index, body fat, and serum glucose, insulin and leptin in rats. Forty male Sprague-Dawley rats were randomly assigned to one of four dietary groups and fed AIN-76 semi-purified basal diets supplemented with 0, 300, 600 or 1200 ppb Cr from CrMet. After 4 weeks on the respective diets, the rats were killed and serum glucose, insulin and leptin concentrations were determined. The CrMet supplementation did not affect weight gain, feed intake or feed efficiency ratio, fasting glucose, insulin or leptin levels among treatment groups. Although final body weight in all treatments were not significantly different, naso-anal length was longer in the 1200 ppb CrMet group than those of control or other groups (p<0.05). The lowest obesity index and body fat were observed in the 1200 ppb dietary group (p<0.05). The obesity index of the rats fed 1200 ppb supplemental CrMet was lower than in the other groups. These results suggest that CrMet supplementation results in a significant decrease in obesity index, possibly by decreasing the body fat that corresponded to increasing CrMet dosage.

Physicochemical Properties of Barley β-Glucan with Alkali Hydrolysis

Sang Hoon Lee,Gwi Yeong Jang,Kee Jong Kim,Heon Sang Jeong,Tae Jip Kim,Mi Ja Lee 한국식품영양과학회 2012 한국식품영양과학회 학술대회발표집 Vol.2012 No.10

Effects of forage level and chromium-methionine chelate supplementation on performance, carcass characteristics and blood metabolites in Korean native (Hanwoo) steers

Sung, Kyung-Il,Ghassemi Nejad, Jalil,Hong, Seok-Man,Ohh, Sang-Jip,Lee, Bae-Hun,Peng, Jing-Lun,Ji, Do-Hyeon,Kim, Byong-Wan BioMed Central 2015 한국축산학회지 Vol.57 No.1

<P>A feeding trial was carried out to determine the effects of chromium methionine (Cr-Met) chelate and forage level over two years, 1<SUP>st</SUP> fattening and 2<SUP>nd</SUP> fattening period on growth parameters, carcass characteristics and blood metabolites of 46 Korean native (Hanwoo, Bos Taurus, BW = 183 ± 44 kg) steers. Treatments were: 1) Steers in the low forage (LF) group were fed diets that consisted of 60% concentrate and 40% forage; 2) Steers in the high forage (HF) group were fed diets that consisted of 40% concentrate and 60% forage. Following the 1<SUP>st</SUP> fattening period, steers (BW = 480 ± 37.6 kg) were randomly assigned to four treatment groups: LF (40 F plus no Cr-Met supplementation in the 2<SUP>nd</SUP> fattening period), LFCM (40LF plus added 400 ppb of Cr-Met during the 2<SUP>nd</SUP> fattening period), HF (60 F plus no added Cr-Met during the 2<SUP>nd</SUP> fattening period) and HFCM (60 F plus added 400 ppb of Cr-Met in the 2<SUP>nd</SUP> fattening period). Dry matter intake of the treatment diets did not differ during the raising and 1<SUP>th</SUP> fattening period (P > 0.05). The ADG in the raising period showed no difference between the 40 F and 60 F groups (P > 0.05). Carcass characteristics including rib-eye area and meat yield index were higher in HF than the other treatment groups (P < 0.05). The HF group tended to show a higher (P = 0.08) marbling score than the LF group whereas the HFCM group showed a higher marbling score than the LFCM group (P < 0.05). HDL was higher and LDL lower in groups fed with Cr-Met than in other groups whereas glucose showed the lowest value in HF group (P < 0.05). Triglyceride (TG), Cholesterol, PUN and total protein (TP) were the same among all treatment groups (P > 0.05). The Insulin concentration in the blood was significantly higher for the HFCM group than for the LF, LFCM and HF groups (P < 0.05). It is concluded that supplementation of chromium-methionine chelate could improve meat quality in beef steers.</P>