H9c2 심근 세포주에서 외인성 nitric oxide가 허혈에 의한 세포 독성에 미치는 영향

정성구,장현용,김명천,고영관,정주호,배영미,박원서,김대중,유영민,김성수,임성빈 대한응급의학회 2001 대한응급의학회지 Vol.12 No.4

Background: Nitric oxide(NO) is known to have protective effects on an ischemic heart and to exert triggering effects on ischemic preconditioning. However, the effects of NO during the ischemic period have not been investigated. To investigate the role of exogenous nitric oxide in a model of ischemic heart cell death, we studied the effects of ischemic preconditioning and ischemia in a normal and an ischemic buffer. Methods: Rat cardiac myoblast cells(H9c2) were cultured in a normal and an ischemic buffered medium. For the ischemic culture of heart cells, the cells were cultured in a dessicator with GasPak for 5 hrs. In ischemic preconditioning, the cells were pretreated with ischemic buffer for 5 min and then perfused with normal medium for 30 min. For the measurement of the cytotoxicity, a MTT(3-4-Sdimethylthiazol-2-yl-2,5-diphenyl tetrazolium bromide) assay was performed. A DAPI(4',6-diamidino-2-phenylindole dihydrochloride) staining procedure and a flow cytometry analysis were performed to confirm apoptotic cell death by ischemia. Results: Cell viability, as determined by using a MTT assay, showed that the preconditioned group treated with NO showed more cell death than with the not-preconditioned groups in both normal and ischemic buffers. But, In normal medium and not-preconditioned groups, NO showed protective effect according to the concentrations(100,1000μM) . No treatment with NO produced the different results. In normal medium, the protective effect of ischemic preconditioning was demonstrated, but no protective effect of ischemic preconditioning could be seen in the case of the ischemic buffer. The DAPI staining and flow cytometry analysis of heart cells showed characteristic apoptotic features. Conclusion: NO added in the ischemic phase had deterious effects on heart cells. Ischemic preconditioning was more harmful than ischemia alone. The toxicity of the cells was characteristic apoptosis.

의식하 진정에 사용된 약물에 관한 임상적 연구

김상렬,김수관,여환호 대한악안면성형재건외과학회 2000 Maxillofacial Plastic Reconstructive Surgery Vol.22 No.4

The purpose of this retrospective study is to investigate the effects of conscious sedation in an outpatient setting. Data from 292 patients (154 males, 138 females), sedated for minor oral surgery, were studied using various parameters including age and sex distribution, as well as according to procedures and each drug group. Results show that this intravenous conscious sedation technique is a safe and effective method of controlling behaviour, but one has to be prepared to deal with any emergencies or complications. The specific results are as follows : 1.Most procedures (265/292) were used to Valium mixed with Demerol and Midazolam mixed with Demerol. 2.Valium revealed a high incidence of pain upon injection and symptoms limited to daily activity and thrombophlebitis. 3.Nausea and vomiting manifested in the procedure using Demerol. 4.The patient manifested comfort similar in response to the same drugs used in another treatment.

최소 영역 원리에 의한 진원도 계산에 관한 연구

강재관,이상용 경남대학교 신소재연구소 1993 論文集 Vol.3 No.-

본 연구에서는 진원도를 계산하기 위해서 측정 결과를 computational geometry를 이용하여 분석하는 새로운 방법을 제시한다. 측정이라는 특성이 고려된 이 방법은 convex hull을 만들어 계산량을 줄이며, 세계적으로 공인된 최소영역기준을 만족하는 해를 보장한다. 특히 본 연구에서 제시된 방법은 기존의 방법들과 달리 on-line dynamic algorithm으로 사용할 수 있기 때문에 측정의 해석 시간뿐만 아니라 검사 비용의 절감 효과도 가져온다. A new algorithm is presented in this paper to assess circularity using the computional geometry. This algorithm is developed by considering the characteristics of measuring operation. It reduces computation time by establishing convex hulls and guarantees the optimal result satisfied the minimum zone criterion. Especially because the proposed algorithm is used as on-line dynamic one, it reduces test cost as well as time in analysis.

토양에서 Napropamide의 흡착과 이동

라덕관,박상숙,김유봉,허동균 순천대학교 공업기술연구소 1998 工業技術硏究所論文集 Vol.12 No.-

This study was to investigate the adsorption and movement of Napropamide in soil. In order to find out the adsorption and movement characteristics of Napropamide in soils of sand loam (soil A), silt clay(soil B), and loam (soil C), batch and column tests were performed with respect to shaking time variation, adsorption isotherm, and breakthrough curve. The shaking time to reach the adsorption equlibrium of Napropamide was 12 hours. Freundlich isotherm was fit for the adsorption of Napropamide in soils. soil B had the highest adsorption coefficient of Napropamide, soil Ca and B followed at adsorption isotherm. The slope of breakthrough curve of soil A had the steep, soil C and soil B followed at the adsorption curve. The moving velocity of Napropamide in soils was the lowest of soil B because of its highest adsorption of Napropamide.

도시경관계획수립을 위한 경관파편화에 관한 연구

정성관,오정학,박경훈 한국지리정보학회 2003 한국지리정보학회지 Vol.6 No.3

최근 부각되고 있는 도시지역의 환경문제 해결을 위해서는 다각적인 접근방법이 요구되는데, 도시지역에 산재해 있는 도시림의 효율적인 관리는 그 방법 중 하나가 될 수 있다. 본 연구에서는 도심지와 인접해 있는 지리적 여건으로 인해 오래전부터 인간의 간섭을 지속적으로 받아온 대구광역시의 앞산자연공원을 대상으로 토지이용 변화 및 경관지수를 사용하여 경관구조 변화에 대해 분석하고자 하였으며, 분석결과를 요약하면 다음과 같다. 첫째, 대상지역의 남쪽으로는 산지연계형의 도시림 특성으로 인해 생태적으로 안정된 환경을 유지하고 있는 반면, 그 외 지역에서는 대구시의 산업화로 인한 이농현상으로 1980년 이전에 도시화가 완료된 것으로 나타났다. 둘째, 경관요소별 면적변화에 따르면, 경작지의 대부분이 도시지역으로 전용된 것으로 나타났으며, 대표적인 경관요소가 소나무림과 농경지에서 혼효림과 도시역으로 변화된 것으로 나타났다. 셋째, 경관지수 분석결과, 조림지에 있어서 인위적인 간섭보다는 산불의 피해임지에 조림된 수종의 조림실패로 인해 활엽수 및 혼효림으로 전환된 것으로 나타났다. Many-sided approach methods are being demanded to solve invironmental problems in urban areas. One of these will be to manage forests scattered in urban areas efficiently. This paper is to grasp the change of land use and landscape indices in Mt. Ap, Deagu, and to analyze the change of landscape structure. Mt. Ap is near Daegu Metropolitan, so under unnatural interferences of human activity persistently. The results of above analysis run as follows: First, the north of the case area is connected to forest, and keeps stable equilibrium ecologically, while the other parts of it suffer from rural exodus and side effects of urbanization which has been completed since 1980. Second, according to the area-rate change of each landscape element, a cultivated areas has been converted into urban one, especially Pinus densiflora forests and paddy fields into mixed forests and urban areas. Finally, most of plantations have been converted into deciduous forests and mixed forests in failure of adption of plants in burned areas rather than owing to factitious interference

Ascorbic acid 가 골조직세포군의 Phosphatase 에 미치는 영향

김상균,김관식,정동균 대한구강생물학회 1987 International Journal of Oral Biology Vol.11 No.2

Several lines of findings suggest that ascorbic acid might influence the function of osteoblasts although no direct evidences were provided. This study was undertaken to investigate the effect of ascorbic acid on bone cells employing 5 fetal rat calvarial cell populations isolated by sequential enzyme digestion. Fetal rat calvaria were treated 5 times consecutively with enzyme mixture of collagenase, trypsin and EDTA for 10, 10, 10, 20 and 20 minutes. Each bone cell population was primarily cultured for 6-7 days and then the effect of ascorbic acid (10 and 100㎍/ml) on the phosphatase level were determined. The observed results were as follows. 1. Population IV and V had characteristics of osteoblast such as high alkaline phosphatase level and low acid phosphatase. 2. Ascorbic acid decreased the acid phosphatase activity of population IV, regardless its concentration while did not affect other cell populations. 3. Alkaline phosphatase activity of population IV was increased significantly by ascorbic acid. 4. Taken together, these results suggest that ascorbic acid may promote the differentiation of osteoblasts and its effect is restricted in osteoblastic population only, not in other type of bone cells.

Group B Streptococci 감염의 임상양상과 Pulsed-Field Gel Electrophoresis를 이용한 유전자형 분석

이승관,이창규,조경진,김상애,김재영,이국성 高麗大學校 倂設 保健大學 保健科學硏究所 2000 保健科學論集 Vol.26 No.1

Pulsed-field gel electrophoresis (PPGE) has been employed as a tool to study correlation between apparent clinical features and genotyping of Group B Streptococci (GBS) infection. The result of serotyping among 45 isolates showed that serotype la, lb, Ⅲ, Ⅳ, Ⅴ, NT (nontypeable) were 15.5%, 11.1%, 35.5%, 2.22%, 20%, 15.5%, respectively. The result of serotyping for infection group of 26 isolates showed that serotype la, lb, Ⅲ, Ⅳ, Ⅴ, NT were 11.5%, 15.4%, 34.6%, 3.8%, 11.5%, 23%, respectively. The result of serotyping for carrier group of 19 isolates showed that serotype la, lb, Ⅲ, Ⅴ, NT were 21%, 5.3%, 36.8%, 31.6%, 5.3%, respectively. Thus, the predominant serotypes of the infection groups were serotype lb and Ⅲ, and those of the carrier groups serotype Ⅲ and Ⅴ. All isolates were characterized by PFGE analysis. Isolates were assigned to three major phylogenetic Divisions (Ⅰ, Ⅱ, Ⅲ) and subsequently to 16 PFGE groups (A[A1, A2, A3], B[B1, B2, B3], C, D[D1. D2, D3, D4, D5, D6], E[E1, E2], F). Seventy five percentages of Division Ⅰ belonged to the infection group. Seventy eight percentages Division Ⅱ of belonged to the carrier group. One hundred percentages of Division Ⅲ belonged to the infection group. The study on the correlation between genotype and serotype among the isolates showed that serotype Ⅲ was predominant in A1 group (5 of 5 isolates), D1 group (4 of 5 isolates), and F group (2 of 2 isolates). In addition, serotype Ⅴ was the sole serotype in C and D5 group. Those results indicated that serotyping and PFGE group analysis were highly correlated.

Medpor 매식후 치유과정에서 Methylprednisolone 이 미치는 영향

김수관,정성수 대한악안면성형재건외과학회 2000 Maxillofacial Plastic Reconstructive Surgery Vol.22 No.2

The purpose of this study is to evaluate the difference in healing of Medpor□ implants of two different thickness (1.5mm and 4.5mm) and the effects of methylprednisolone on the healing process. Light micro-scopic and scanning electron microscopic examinations, and hardness measurement were made in 100 rats 2, 4, or 8 weeks postoperatively. The 1.5mm thin implants were taken better than the 4.5mm thick implants. The inflammatory responses were reduced after application of methylprednisolone with arrangement and amount of bone matrix deposited being more irregular and reduced in bulk than in the control group. To hasten postoperative healing after Medpor□ implantation, the thinner material should be used and steroid injection should be avoided. Key Words : Medpor□, methylprednisolone

연속식 오존처리공정을 이용한 낙동강 상수원수의 정수처리 효과

임영성,강관호,이홍재,서동철,박문수,허종수,조주식 한국환경농학회 2002 한국환경농학회지 Vol.21 No.3

낙동강 상수원수를 대상으로 연속적 오존처리에서 오존주업농도에 따른 유기물 분해 특성을 조사하기 위하여 오존처리에 의한 DOC성분변화, UV254/DOC의 변화와 소비오존에 따른 DOC 제거효율을 조사한 결과는 다음과 같다. 유입원수의 수질변화에 따라 다소 차이는 있었으나 전반적으로 오존주입농도가 증가할수록 DOC 처리효율도 증가되었다. 오존처리에 따른 DOC 성분변화는 전오존을 3 mg/L농도로 처리시 TDOC, ADOC, A&BDOC 및 NRDOC는 각각0.6, 0.2, 0.1 및 1.0 mg/L 정도 오존처리에 의하여 감소되었으나 BDOC는 0.8 mg/L 증가하였다. UV254/DOC의 분율은 오존주입농도를 1, 3 및 5 mg/L 처리하였을 때 전오존의 경우는 각각 0.048, 0.044 및 0.037 이였고, 후오존의 경우는 각각 0.018, 0.015 및 0.012로 나타나 오존주입농도가 증가할수록 감소하는 경향을 보였다. 초기 DOC mg당 소비되는 오존량에 대한 DOC 제거율은 전오존과 후오존처리의 초기 DOC에 대한 소비오존량이 각각 약 1.5와 2.3일 때 각자 25%와 32%로서 최대의 DOC 처리효율을 나타내었다. 낙동강 상수원수에 전오존을 처리할 경우 오존주입량은 초기 DOC mg당 소비된 오존이 1.5 mg 이내의 농도가 되도록 주입하고, 후오존은 유입수인 모래여과수의 초기 DOC mg당 소비된 오존이 2.3 mg이내의 농도가 되도록 조절하여 주입하면 적당할 것으로 생각되었다. This study was carried out to evaluate the pollutant removal efficiencies of the advanced drinking water treatment using ozonation process. For raw water. Nakdong River was used. By conducting continuous ozonation experiments, the following results were obtained. Efficiency of water treatment was enhanced with increase in ozone dosage. When ozone dosage of 3 mg/L was used, preozonation of raw water reduced TDOC, ADOC, A&BDOC and NRDOC as much as 0.6 mg/L, 0.2 mg/L, 0.1 mg/L and 1.0 mg/L, respectively. On the other hand, content of BDOC Increased at the level of 0.8 mg/L. Ratio of UV254 absorbance to DOC content by the preozonation with 1, 3 and 5 mg/L dosage were 0.048, 0.044 and 0.037, respectively. In case of postozonation, it were 0.018, 0.015 and 0.012, respectively. When the ratios of consumpted ozone content to 1 mg of initial DOC were 1.5 and 2.3 in preozonation and postozonation treatment, respectively, the highest DOC removal rates of 25% and 32% were obtained by the continuous ozonation.

加壓浮上法에 依한 活性슬러지 混合液의 固液分離에 關한 硏究

梁相鉉,羅德寬 全北大學校 1985 論文集 Vol.27 No.-

An effective technique of sludge separation is required for concentrated activated sludge process. The dissolved-air flotation (DAF) has been shown to be efficient process for sludge separation. The factors affecting DAF process for activated sludge separation are type and concentration of sludge, air/solid ratio, ratio of pressurized water flow, pressure, sludge detention time, temperature, sludge and hydraulic loading rate, recycle flow rate of sludge and type and quantity of chemical aid. In order to study the optimal operation condition for sludge separation, the influence factors such as type and concentration of sludge, ratio of pressurized water flow and pressure are investigated by batch and continuous reactor experiments of DAF and sedimentation test. The results obtained through experimental investigation are as follws; 1. For the bulking and concentrated sludge, DAF is more effective than sedimentation for the sludge separation. 2. In DAF, the critical ratio of pressurized water flow exist. The critical value varies with the pressure in the tank. That is, according to the pressure changes from 3 to 5 kg/㎠, the critical value varies from 0.25 to 0.67 accordingly. 3. Pressure affects the ratio of pressurized water flow, but it does not show any influence upon the DAF efficiency directly. 4. Continuous experimental rerults was not better than those of batch.