악성종양에서 혈청 Intercellular Adhesion Molecule-1의 혈청

조덕연,김현수,박상준,김종숙,최지영,윤환중,김삼용 충남대학교 의과대학 지역사회의학연구소 1995 충남의대잡지 Vol.22 No.1

Intercellular adhesion molecule-1 (ICAM-1) is a glycoprotein serving as ligands for leukocyte intergrin receptors, i.e. LFA-1, MAC-l. It has been suggested that the expression of ICAM-1 and the levels of circulating ICAM-1 were increased in several malignancies. We measured serum ICAM-1 by ELISA in patients with stomach cancer (n=25), acute lymphoblastic leukemia (n=7), non-Hodgkin's lymphoma (n=14), and metastasis of unknown origin (n=5). Serum levels of circulating ICAM-1 in all malignancies were significantly higer than in normal controls. And serum level of ICAM-1 in stomach cancer ptients having metastatic disease was higher than in patients with localized disease (p=0.027). These results suggest that elevated serum ICAM-1 is a rather common feature for malignancies, not unique for certain types of cancer such as malignant melanoma. It remains to be clalified whether marked increase of serum ICAM-1 in metastatic stomach cancers simply reflect tumor burden or this molecule plays a role in progression in stomach cancer.

국가기술자격 검정의 위탁 확대를 위한 민간기관의 자격검정 능력 실태 분석

김덕기,김상진,이동임 한국직업능력개발원 2006 직업능력개발연구 Vol.9 No.1

본 연구의 목적은 기존의 정부주도형 국가기술자격제도의 구조적 한계를 극복하고 수요자 중심의 자격제도 시행이 효과적으로 이루어질 수 있도록 검정의 민간 위탁 확대 시행방안을모색하는 데 있다. 이를 위해 이 연구에서는 위탁 대상 기관의 현황과 이들 기관의 자격검정 관리·운영 능력 실태를 조사하고, 이에 근거하여 국가기술자격검정 위탁의 효율적인 시행 방안을 제시하였다. 위탁 대상 기관의 현황 및 실태는 사업주 단체, 국가자격 검정기관, 국가공인민간자격관리자, 공공기관의 교육훈련 또는 자격검정 담당자를 대상으로 한 설문조사와 면담조사를 통해 이들 기관의 국가기술자격 검정의 위탁 참여 의사, 검정 관리·운영 능력, 위탁 시의 문제점 등을 조사하였다. 연구결과 민간기관의 국가기술자격검정의 위탁 수행에 대한 의지는 높으나, 검정 관리·운영능력을 고려할 때 국가기술자격 검정의 위탁을 단기간 내에 확대 시행하기에는 어려움이 있는 것으로 나타났다. 그러나 이미 국가자격검정을 전국적으로 시행하고 있는 민간기관의 경우해당 분야의 전문성과 대표성을 확보하고 있고, 전국적인 검정 시행 경험과 검정 인프라를 구축하고 있는 것으로 조사되어, 상대적으로 적은 예산지원과 기술지원으로 국가기술자격검정의 위탁 확대 시행이 가능한 것으로 나타났다. The government-led national technical qualification system is said to have failed in meeting the demands of the rapidly changing labor market due to its rigidity. To better satisfy customer demands, including those of industries, the Korean government has encouraged the private sector to participate and take a more active role improving the qualification system, and has extended the entrustment of the national technical qualification testing to private organizations. The purpose of this study is to propose an implementation plan for the government to help promote efficient entrustment of national technical qualification testing to private organizations. The research methods used in the study were questionnaire survey and interviews with those in charge of qualification testing or education and training in national qualification testing institutes, industry or occupational associations and government-authorized private qualification institutes. The main purpose of the survey and interviews was to investigate private organizations‘ capacity to carry out national qualifications testing and their will to participate as national technical qualifications testing centers. The results indicate that most of private organizations hope to become testing centers of national technical qualifications, It, however, seems to be too premature to extent the entrustment of national technical qualification testing to private organizations considering their lack of ability to carry out the testing task of the national technical qualifications.

염증성 근섬유모세포종의 증례보고

은상아,박혁,차인호,김현실,정호걸,김기덕,박창서 대한구강악안면방사선학회 2006 Imaging Science in Dentistry Vol.36 No.3

The inflammatory myofibroblastic tumor (inflammatory pseudotumor) is a rarely occurring soft tissue lesion of unknown etiology. It can be of any location, but commonly it is found in lungs. It has been considered as a nonneoplastic reactive inflammatory lesion, but nowadays, confusion and dispute about its character is increasing due to its high recurrence rate and metastasis. We present a patient who had been diagnosed with an inflammatory pseudotumor in the right maxilla area, 1 year before visiting our hospital. After that, her pain and swelling did not resolved and she visit our hospital. On radiographic examination, aggressively infiltrative growth of the lesion with destruction of adjacent bony structure was noted. We found unusual aggressiveness of the inflammatory myofibroblastic tumor of the head and neck region. Because the typical behavior of the inflammatory myofibroblastic tumor is not defined yet, we recommend the surgical excision of the lesion and close follow-up.

사이토카인과 Lipopolysaccharide 자극에 의한 RAW 264.7 세포주의 nitric oxide 생성

김영덕,전창덕,이병순,이복수,박석돈,백상기,정헌택 충남대학교 생물공학연구소 1993 생물공학연구지 Vol.3 No.-

Macrophages have been implicated as a major class of effector in the host response to neoplasia. Cells of the monocyte-macrophage lineage are known to exhibit tumoricidal activity following stim-ulation by γ-interferon, tumor necrosis factor, BCG and bacterial products such as lipopoly-saccharide (LPS). While the mechanism involved remain obscure, the generation of reactive nitrogen intermediate (RNI) by activated macrophages is considered a maior participant in mediating the tumorstatic effect. But much of what is known about the induction and release of RNI has been elucidated by using freshly isolated cells from blood and other tissues of experimental animals. In this study, we used a murine macrophage cell line, RAW 264.7, and found that these cells showed above 99% positive of pan macrophage marker by immunohistochemical staining. These cells could produce nitric oxide (NO), when incubated with γ-IFN or poly I:C. Incubation of RAW 264.7 cells with γ-IFN for 48 hous in the presence of LPS agumented NO release in a dose dependent manner. Whereas, treatment of anti-TNE-α antibody or antisense TNF-α oligodeoxynucleotide inhibited the release of NO_2 by γ-IFN plus LPS activated macrophages. The production of NO was also inhibited reversibly or irreversibly by N^GMMA,NAA,arginase or DPI. Thease data suggest that RAW 264.7 cell line may be useful for the in vitro evalulation of biological response modifiers as well as the study of signal pathway of NO release by macrophages.

동천의 정화능력 평가

라덕관,박상숙,김기성,김영규,김성진 순천대학교 1999 순천대학교 자연과학논문집 Vol.18 No.2

This study was carried out to investigate the self-purification capacity of Dongcheon in Sunchon. According to water quality of Dongcheon, the mean concentrations of BOD and DO were 0.7∼1.6㎎/ℓ and 7.1∼8.0㎎/ℓ, which correspond to the grade Ⅱ of water quality standards. The ratio of BOD loading inflowed from Sunchon-city, Seocheon and Okchoen were measured 4.8%, 52.1% and 23.7%, respectively. The deoxygenation constant(k_1) and reaeration constant(k_2) of Dongchoen were estimated as 3.19day^(-1) and 2.181day^(-1). The results of DO distribution analysis by Streeter-Phelps equation showed that critical point was 12.1㎞ downstream, and then DO concentration was 6.94㎎/ℓ. In addition, the self-purification capacity assessment indicated that BOD loading to maintain over 5㎎/ℓ of DO should be less than 0.925ton/day.

토양에서 Napropamide의 흡착과 이동

라덕관,박상숙,김유봉,허동균 순천대학교 공업기술연구소 1998 工業技術硏究所論文集 Vol.12 No.-

This study was to investigate the adsorption and movement of Napropamide in soil. In order to find out the adsorption and movement characteristics of Napropamide in soils of sand loam (soil A), silt clay(soil B), and loam (soil C), batch and column tests were performed with respect to shaking time variation, adsorption isotherm, and breakthrough curve. The shaking time to reach the adsorption equlibrium of Napropamide was 12 hours. Freundlich isotherm was fit for the adsorption of Napropamide in soils. soil B had the highest adsorption coefficient of Napropamide, soil Ca and B followed at adsorption isotherm. The slope of breakthrough curve of soil A had the steep, soil C and soil B followed at the adsorption curve. The moving velocity of Napropamide in soils was the lowest of soil B because of its highest adsorption of Napropamide.

삼변수 kappa 분포를 적용한 매년 최대연속강우량 계열의 빈도해석

박상덕,이원환 연세대학교 산업기술연구소 1991 논문집 Vol.23 No.2

The total depths of a rainfall event may be important in planning of flood control. This study is to analyse the applicability of three parameter kappa distribution for annual maximum series of continuous rainfall at Seoul, Incheon, Busan, and Kwangju meteorological station and to compare to three parameter lognormal distribution, extremal type 1 distribution, Pearson type 3 distribution, and log-Pearson type 3 distribution. As results of this study annual maximum series of continuous rainfall are random data and are fitted by three parameter kappa distribution. For large return period the probable continuous rainfall depths by three parameter kappa distribution are more than those by other distribution, for small return period the rainfall depths are less. The probable continuous rainfall depths by extremal type 1 distribution are computed less than by other distribution. The type of best fitted probable distribution for a rainfall data must be chosen by considering the X² value and the probable rainfall depths for large return period.

오존, 오존/超音波 및 오존/紫外線을 利用한 廢水處理

金基成,羅德寬,李禹範 순천대학교 공업기술연구소 1988 工業技術硏究所論文集 Vol.2 No.-

The wasterwater treatment using combination system of ozone/ultrasonics or ozone/ultraviolets compared with the ozone was able to increase the ozone oxidation rate and hydrogenperoxide generation. But, the patterns of interactions between ozone and ultrasonics or ultraviolets are very complex, and reaction mechanism are not understood completely. Thus, this study was conducted to evaluate fundamental factors of effective ozonization when this combination works were carried out.

대표적 근경류 생약의 방사선 방호효과

김성호,조성기,권오덕 대한방사선 방어학회 2001 방사선방어학회지 Vol.26 No.1

한의학에서 근경류(rhizomata)에 분류되어 있으며 각종 보익탕제에 주로 사용되는 대표적 생약인 백출(Baizhu, Atractylodes japonica), 천궁(Chuanxiong, Cnidium officinale), 산약(Shanyao, Discorea japonica) 및 승마(Shengma, Cimicifuga heracleifolia)의 방사선 방호효과를 확인하기 위해서 고선량 및 저선량의 방사선을 마우스에 조사하고 소장움 생존, 내재성 비장집락형성 및 apoptosis 유발 등을 관찰하였다. 방사선조사전 승마 투여군에서 소장움세포의 생존율을 증가시켰으며 내재성 비장집락 형성 시험에서는 천궁 투여군에서 평균치의 증가가 관찰되었고 천궁 및 승마는 저선량 방사선에 의한 apoptosls 형성을 억제시켰다. 이상의 결과에서 승마 및 천궁의 방사선 방호효과를 조혈세포의 생존과 회복, 소장움세포 생존을 통하여 확인하였으며 이는 독성이 적은 천연물이라는 관점에서 방사선 방호 식품으로서 적용이 가능할 것으로 사료된다. We performed this study to determine the effect of Baizhu(Atractylodes japonica), Chuanxiong(Cnidium officinale), Shanyao(Discorea japonica) and Shengma(Cimicifuga heracleifolia), as Oriental rhizomata herbs, on jejunal crypt survival, endogenous spleen colony formation and apoptosis in jejunal crypt cells of mire irradiated with high and low dose of γ-radiation. Shengma was effective in intestinal crypt survival(p<0.05). The frequency of radiation induced apoptosis was also reduced by pretreatment with Chuanxiong and Shengma(p<0.05). Although the mechanisms of this effect remain to be elucidated, these results indicated that Shengma might be a useful radioprotector, especially since it is a relatively nontoxic natural product.

정상과 갑상선 종양조직에서 사람 IGF-I 유전자의 발현

김성운,장현하,박상미,김덕윤,우정택,양인명,김진우,김영설,김광원,고석환,홍성화,최영길 경희대학교 유전공학연구소 1993 遺傳工學論文集 Vol.5 No.-

Many of the growth-promoting properties of growth hormone(GH) are mediated by insulin-like growth factor-I(IGF-I), a highly conserved circulating 70-amino acid peptide. Recent studies have shown that multiple mechanisms influence IGF-I gene expression, including transcription from two promoters, alternative RNA splicing, and variable polyadenylation. In thyroid tissue, thyroid stimulating hormone(TSH) and IGF-I are the most possible candidates for follicular cell proliferation and hypertrophy. Actually IGF-I had autocrine and paracrine effect for tissue growing. We prepared thyroid tumor tissue mRNAs using single step method for detecting IGF-I levels according to different tissues, i.e., thyroid adenoma or papillary thyroid carcinoma. We used Northern blot analysis for IGF-I mRNA and RNase protection assay (RPA) for IGF-I transcription start sites. For Northern blot, we used whole human IGF-I cDNA as a DNA probe and for RPA, we used IGF-I exon 1 containing noncoding promoter 1 as a riboprobe. We got good RNA bands from Northern blot analysis around 1 kb (IGF-IA) and 7.5 kb (IGF-IB) region. To clarify the amount of both IGF-IA and IB mRNAs, we measured autoradiographied signal of IGF-I mRNAs bands using densitometer. In IGF-IA signals, there's no change among liver and thyroid tissues, but in case of IGF-IB mRNA bands, the signal was markedly increased in thyroid carcinoma tissues than that of normal thyroid tissue (85% vs 14%). In the study of RPA, all thyroid tissues used the same transcription start sites as those of liver's. We concluded that that this different regulation of IGF-I mRNA was originated from tissue specificity. That meant some tissue specific transcription factor/s were related to tissue IGF-I expression.