두송실의 치아탈회 및 Glucosyltransferase 활성억제효과

남상해(Sang-Hae Nam),장대식(Dae-Sik Jang),양민석(Min-Suk Yang) 한국식품영양과학회 1999 한국식품영양과학회지 Vol.27 No.6

두송실 추출물의 S. mutans에 의한 치아탈회 및 glucosyltransferase의 저해활성을 조사하였다. 두송실 methanol 추출물과 n-hexane분획물에서 각각 70.13, 74.93%의 치아탈회 억제효과를 타나내었다. GTase활성의 저해실험에서도 methanol추출물과 n-hexane분획물에서 각각 86.6, 89.5%의 glucan합성을 저해하였다. 또한 S. mutans가 생성하는 GTase를 SDS-PAGE로서 확인해 본 결과에서도 methanol추출물과 n-hexane, chloroform분획물에서 GTase로 추정되는 65KD부근의 band가 나타나지 않았다. We investigated the inhibition effects of teeth decalcification and glucosyltransferase(GTase) activity on the Juniperus rigida S. et Z. Teeth decalcifications by Streptococcus mutans were respectively inhibited to 70.13, 74.93% on methanol and n-hexane fractions. In the inhibition test of GTase activities by solvent fractions of J. rigida, they were respectively inhibited to 86.6, 89.5% to a similar degree. And in the result to identify GTase produced by S. mutans with SDS-PAGE, the band near 65KD estimated as GTase did not show in the lanes of methanol, n-hexane and chloroform fractions.

산국 추출물의 항균력

남상해(Sang Hae Nam),양민석(Min Suk Yang) 한국응용생명화학회 1995 Applied Biological Chemistry (Appl Biol Chem) Vol.38 No.3

Antibacterial activity test of solvent fractions, sesquitetpenoid lactones, and Compound Ⅰ and Ⅱ extracted from Chrysanthemum bmeale M. and Chrysanthemum indicum L. were performed against four microorganisms. Among the tested substances, antibacterial activities were appeared against B, subtilis and V. parahaemolyticus at the chloroform fraction, sesquiterpenoid lactones and Compound Ⅰ extracted from C. foreale. But chloroform fraction and sesquiterpenoid lactones eactracted from C. indicum were showed weakly than those of C. boreale. Compound Ⅱ and all fractions extracted from C. indicum were not appeared against the all tested microorganisms.

Characteristics of Allergy Inducing Materials Isolated from Rhus verniciflua Stokes on BALB/c Mice

Sang Hae Nam(남상해),Ok Soo Joo(주옥수),Jun Lee(이준) 한국생명과학회 2009 생명과학회지 Vol.19 No.7

옻 allergy 유발물질을 분리하고 그 특성을 연구하기 위하여 국내산, 중국산 및 일본산 생 옻을 사용하였다. 옻의 crude extracts로부터 crude urushiol을 정제하고, 다시 allergy 유발물질로 알려져 있는 3종의 urushiol congeners를 분리하였다. 분리한 urushiol congeners는 RV-1 (C₂₁H₃?O₂, MW 314.462), RV-2 (C₂₁H₃₂O₂, MW 316.240) 및 RV- (C₂₃H₃₄O₂, MW 342.515)였으며, 전체의 80%에 해당하였다. RV-1의 함량은 중국산에서 70.07%로 가장 많았으며, 일본산에서 62.38%로 가장 적었다. 반면에 RV-2는 일본산은 9.25%로서 국내산과 중국산의 4.28 및 3.09%에 비하여 약 2~3배 정도 많았다. Allergy 유발특성을 보면, RV-1은 초기유발강도가 강하고 지속적이어서 회복속도도 늦었다. RV-2는 초기유발강도는 약하지만 회복속도는 늦었다. 반면에 RV-3는 초기유발강도는 비교적 약했지만, 감작 후 48시간째에 가장 강하게 유발되었고, 회복속도가 빨라서 72시간이 경과하였을 때에는 거의 대조군 수준으로 회복되었다. 따라서 allergy 유발에 가장 큰 영향을 미치는 urushiol은 RV-1이었으며, RV-1의 함량이 가장 많은 중국산이 allergy의 유발강도가 가장 강하였고 회복속도도 늦음을 알 수 있었다. We purified crude urushiol from natural lacquer produced in Korea, China, and Japan and then isolated several urushiol congeners known to induce allergic reactions. The 3 major kinds of urushiol congeners that were isolated and purified were RV-1 (C₂₁H₃?O₂, M.W. 314.462), RV-2 (C₂₁H₃₂O₂, M.W. 316.240) and RV-3 (C₂₃H₃₄O₂, M.W. 342.515), occupying 80% of total crude urushiol. The content of RV-1 was the highest in natural lacquer from China at 70.07%, and was the lowest in that from Japan at 62.38%. However, the content of RV-2 in natural lacquer from Japan was 9.25%, 2~3 times higher than those from Korea (4.28%) and China (3.09%). As an allergy inducing character, RV-1 had strong inducing power and durability in the primary stage, showing slow recovery. RV-2 had weak power in the primary stage and also showed slow recovery. Although RV-3 had comparatively weak power at the primary stage, it induced the strongest allergy contact dermatitis after 48 hr. However, it recovered to nearly the same level as control group 72 hr after sensitization time. Accordingly, we found out that RV-1 is the most influential of urushiol congeners in inducing allergic reactions, natural lacquer from China having the most inducible strength and slowest recovery compared to those from Korea and Japan.

산국으로부터 분리한 Sesquiterpene Lactones의 흰쥐 복수암에 대한 효과

남상해(Sang-Hae Nam),최상도(Sang-Do Choi),최진상(Jine-Shang Choi),장대식(Dae-Sik Jang),최상욱(Sang-Uk Choi),양민석(Min-Suk Yang) 한국식품영양과학회 1997 한국식품영양과학회지 Vol.26 No.1

산국의 꽃으로부터 2종의 sesquiterpene lactones을 분리정제하였으며, 각각 Compound Ⅰ과 Ⅱ로 명명하였으며, 이 두 물질을 Sarcoma180에 감염된 ICR mouse에 대하여 항암효과실험을 수행한 결과 Compound Ⅰ과 Ⅱ의 투여농도가 10㎎/㎏일 때 수명연장효과가 각각 143%, 134%로 나타났다. 또한 Compound Ⅰ과 Ⅱ를 1㎎/㎏ 씩을 섞어서 투여하였을 때에 158%의 수명연장효과가 나타났다. For the investigation of antitumor agents, two kinds of sesquiterpene lactones were isolated and purified from Chrysanthemum boreale M. and designated as Compound Ⅰ and Ⅱ. And then in vivo antitumor test of the sesquiterpene lactones was carried out against ICR mice. In vivo test against Sarcoma180 implanted ICR mice, life prolongation effects of Compound Ⅰ and Ⅱ were showed as 143% and 134% at the dose of 10㎎/㎏, respectively. Besides, at the tested group mixed Compound Ⅰ and Ⅱ each 1㎎/㎏ was showed rather high life prolongation effect as 158%.

산국으로부터 항암활성 성분의 분리

남상해(Sang Hae Nam),양민석(Min Suk Yang) 한국응용생명화학회 1995 Applied Biological Chemistry (Appl Biol Chem) Vol.38 No.3

Sixty kinds of medicinal plants were examined upon the cytotoxicity against L1210 mouse leukemia cells. Isolation and purfication of effective antitumor substances from Chrysanthemum boreale had been performed which appeared strong cytotoxicity, In cytotoxicity test of the each solvent fractions, ED_(50) values of chloroform fraction against L1210, K562 and A549 cells were shown as 3.98, 4.28, 3.84 (㎍/㎖), respectively. Compound Ⅰ and Ⅱ were purified from the chloroform fraction. Between the purified compounds, ED_(50) values of Compound Ⅰ against L1210, K562 and A549 cells were shown as 0.55, 0.0003, 0.001 (㎍/㎖), respectively. Whereas Compound Ⅱ was shown as 4.79 against K562.

투과 증발을 위한 기능성 키토산 막

황해영 ( Hae Young Hwang ),임지원 ( Ji Won Rhim ),남상용 ( Sang Yong Nam ) 한국키틴키토산학회 2005 한국키틴키토산학회지 Vol.10 No.4

술폰화된 SEBS 이온 전도성 고분자 막의 DMFC 특성

황해영 ( Hae Young Hwang ),정미애 ( Mi Ae Jeong ),남상용 ( Sang Yong Nam ) 한국공업화학회 2007 응용화학 Vol.11 No.2

CHECKMATE$^{TM}$를 이용한 일일 출력 검증의 유용성 평가

조한상,남상수,박혜진,김미화,박안태,Cho, Han-Sang,Nam, Sang-Soo,Park, Hae-Jin,Kim, Mi-Hwa,Park, An-Tae 대한방사선치료학회 2011 대한방사선치료학회지 Vol.23 No.1

Purpose: In this study, we tried to check the usefulness of two Linear Accelerators, Clinac IX and 21EX (Varian, Palo Alto, CA), which are equipped in Ajou Medical Center. From 2008 to 2010, we evaluated the error range of Absolute Dose based on the daily output, which was measured by CHECKMATE$^{TM}$ (Sun Nuclear, Melbourne, FL). Materials and Methods: For Daily Q.A, photon beams of two linear accelerators, 21EX and IX (6 MV and 10 MV, respectively) were measured daily by using CHECKMATE$^{TM}$ just before the treatment began, while the absolute dose was measured biweekly by using water phantom. We analyzed the data of measured values from the daily Q.A and the absolute dose from 2008 to 2010 for 21EX, and from 2009 to 2010 for IX. We utilized Excel 2007 (Microsoft, USA) to evaluate Average, Standard deviation and Confidence level of the data. Furthermore, in order to check the measured values of CHECKMATE$^{TM}$ and the significance of absolute dose, each error value was compared and analyzed. Results: During the observation period, the output of two equipment's absolute dose increased in process of time and in both 6 MV and 10 MV, there was a similar increasing trend. In addition, the error rate of the measured value of CHECKMATE$^{TM}$ and the value of absolute dose were under 0.34, which means that there is a similarity relationship between the two measured values. After checking that the measured value of CHECKMATE$^{TM}$ increased, We measured the absolute dose to adjust that. When the error range was close to 2~3%, the number of changing the output was four for 21EX and three for IX. Conclusion: As a result of measuring and analyzing the daily output changes for two years by using CHECKMATE$^{TM}$, we could find that there is a significance between the output which we should obey during Q.A, and the measured value of absolute dose within the error tolerance of 2~3%. Thus, the use of CHECKMATE$^{TM}$ can be positively considered for more efficient and reliable daily output verification of linear accelerator. It can also be a good standard for other medical centers to understand the trends of linear accelerator and to refer to for the correction of each output.

국내산 녹차 및 후발효차 추출물의 항산화 효과

손미예,김성희,남상해,박석규,성낙주,Shon Mi-Yae,Kim Sung-Hee,Nam Sang-Hae,Park Seok-Kyu,Sung Nak-Ju 한국생명과학회 2004 생명과학회지 Vol.14 No.6

국내산 녹차와 후발효차의 총 플라보노이드와 페놀 성분 및 몇 가지 항산화능을 분석하였다. 총 플라보노이드 함량은 녹차 추출물$(413.3\;{\mu}g/g)$과 후발효차 추출물$(405.7\;{\mu}g/g)$ 이 비슷하게 함유하였으며, 총 페놀 함량은 후발효차 추출물 $(23.5\;{\mu}g/g)$보다 녹차 추출물$(46.8\;{\mu}g/g)$이 더 높게 포함되었다. 녹차 추출물의 catechin류에서는 EGC, GC, catechin, catechol 및 EGCG가 검출되었는데, 이 중 EGCG 함량이 가장 많았으며, 후발효차의 열수 추출물에서는 EGCG가 검출되지 않았다. SOD 유사활성은 추출물의 농도가 증가할수록 비례적으로 그 활성이 증가하였다. Hydroxyl radical의 소거능은 각 추출물의 $3000\;{\mu}g/ml$농도에서 $60\%$ 이상을 나타내었고, $\beta-carotene-linoleate\;system$에서는 L-ascorbic acid와 비슷한 경향을 보였으며, 후발효차 추출물보다 녹차 추출물에서 효과적이었다. The beneficial effects of green and fermented tea are generally attributed to some antioxidant activities including superoxide dismutase (SOD)-like ability and scavenging activity originated from their phenolic compounds and flavonoids. Content of total flavonoid of green tea $(413.3\;{\mu}g/g)$ was similar to those of fermented tea $(405.7\;{\mu}g/g)$. Content of total phenol of green tea $(46.8\;{\mu}g/g)$ was higher than those of fermented tea $(23.5\;{\mu}g/g)$. Major catechin compounds of hot water extract in green tea was EGCG, including EGC, Gc, catechin and catechol. EGCG was not detected .in fermented tea. SOD-like ability was increased in proportional to added concentration of hot water extract. The scavenging activities of hydroxyl radical at $3000\;{\mu}g/ml$ of green and fermented teas were found up to $60\%$. Hot water extract of green tea was more effective in scavenging activity than that of fermented tea.

구절초 꽃의 항균성 물질

장대식,박기훈,최상욱,남상해,양민석,Jang, Dae-Sik,Park, Ki-Hun,Choi, Sang-Uk,Nam, Sang-Hae,Yang, Min-Suk 한국응용생명화학회 1997 Applied Biological Chemistry (Appl Biol Chem) Vol.43 No.1

Chrysanthemum속(屬) 식물의 성분 및 생리활성물질에 대한 연구의 일환으로, 구절초 꽃의 chloroform 분획물을 재료로 B. subtilis, 및 V. aureus 및 v. parahaemolyticus에 대한 activity-guieded fractionation을 실시하여 항균성 물질 두가지를 얻었다. 항균력실험 결과,화합물I은 $100\;{\mu}g/disk$의 농도에서 세가지균주 모두에 활성이 있었고 화합물 II는 B. subtilis와 V. parahaemolyticus에만 활성이 나타났다. 화합물 I과 화합물 II는 현재 식품보존료로 사용되는 benzoic acid 및 sorbic acid와 비교해볼 때, B. subtilis와 v. perahaemolyticus에 대하여 대략 5배정도 강한 항균력을 나타내었다. $^1H,\;^{13}C\;NMR,\;DEPT,\;^1H-^1H\;COSY,^{13}C-^1H\;COSY$ 및 Mass spectrum 등을 분석한 결과, 화합물 I과 II는 sesquiterpene lactone인 angeloylcumambrin B($C_{20}H_{25}O_5,$ MW=346)와 cumambrin A($C_{17}H_{25}O_5,\;W=346$)로 각각 동정되었으며 이들은 구절초에서는 처음 분리되었다. In orther to isolate antibacterial substances from the flower of Chrysanthemum zawadskii Herbich var. latilobum Kitam., the chloroform fraction was fractionated according to the activity against B. subtilis, S. aureus and V. parahaemolyticus. Two antibacterial substances were isolated and purified by preparative TLC and recrystallization. Compound I showed activity against all the tested bacteria and compound II exhibited the activity against B. subtilis and v. parahaemolyticus except S. aureus. Compared to benzoic acid and sorbic acid which are being used as food preservatives, compounds I and II showed about five-fold stronger antibacterial activity against B. subtilis and V. parahaemolyticus. On the basis of spectrometric studies including $^1H-NMR,\;^{13}C-NMR,\;DEPT,\;^1H-^1H\;COSY,\;^{13}C-^1H\;COSY$ and Mass, compounds I and II were identified as angeloylcumambrin B and cumambrin A, respectively. This is the first report of the isolation of angeloylcumambrin B and cumambrin A from this plant.