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鹿茸이 卵巢摘出로 유발된 흰쥐의 骨多孔症에 미치는 影響
沈相度,安德均 대한본초학회 1999 大韓本草學會誌 Vol.14 No.1
The purpose of this study is to examine what are the effects of the Cervi Cornu on the aged ovariectomized rat model of postmenopausal osteoporosis. Experimantal groups(UP, MP) were administered water extract of upper portion(ash weight<25%) and middle portion(25%<ash weight<30%) of Cervi Cornu for 55 days. Control group(CO) was administered normal saline. And we estimated osteocalcin, calcium, ALP and phosphorus in serum, calcium and creatinine in urine, organ weight and changes of bone. The results of the experiment are as follows, 1. Body weight in upper portion and middle portion of Cervi Cornu group were increased in comparison with control group, but showed no efficiency. 2. The level of serum osteocalcine in upper portion of Cervi Cornu group was increased in comparison with control group, but showed no efficiency. The level of serum calcium, ALP, phosphorus, S-GOT and S-GOT in upper portion and middle portion of Cervi Cornu group showed no significant change. 3. Urinary volume in upper portion and middle portion of Cervi Cornu group showed a significant increase. The level of urine creatinine in upper portion and middle portion of Cervi Cornu group showed a significant decrease. The level of urine calcium to creatinine ratio in upper portion of Cervi Cornu showed a significant increase. 4. The weight of herat, liver, spleen and kidney in upper portion and middle portion of Cervi Cornu group showed no significant change. The weight of uterus in upper portion of Cervi Cornu group was increased in comparion with control group, but showed no efficiency. 5. The weight of tibia ash and the femur length in upper portion and middle portion of Cervi Cornu group showed a significant increase. According to the above results. effects of the Cervi Cornu on the aged ovariectomized rat model showed a efficiency in elevation of bone density by increasing bone mass and in prevention of renal function failure by ovariectomy. So Cervi Cornu can be used for prevention and curing of postmenopausal osteoporosis. As for it's effects, continuous clinical study is needed.
Yang, San-Duk,Jang, Se-Song,Han, Jeong A.,Park, Hyun-Seok,Kim, Jong-Il Yonsei University College of Medicine 2016 Yonsei medical journal Vol.57 No.4
<P><B>Purpose</B></P><P>Lymphatic invasion (LI) is regarded as a predictor of the aggressiveness of ovarian cancer (OC). However, LI is not always the major determinant of long-term patient survival. To establish proper diagnosis and treatment for OC, we analyzed differentially expressed genes (DEGs) for patients with serous epithelial OC, with or without LI, who did or did not survive for 5 years.</P><P><B>Materials and Methods</B></P><P>Gene expression data from 63 patients with OC and LI, and 35 patients with OC but without LI, were investigated using an Affymetrix Human Genome U133 Array and analyzed using The Cancer Genome Atlas (TCGA) database. Among these 98 patients, 16 survived for 5 years or more. DEGs were identified using the Bioconductor R package, and their functions were analyzed using the DAVID web tool.</P><P><B>Results</B></P><P>We found 55 significant DEGs (<I>p</I><0.01) from the patients with LI and 20 highly significant DEGs (<I>p</I><0.001) from those without it. Pathway analysis showed that DEGs associated with carbohydrate metabolism or with renal cell carcinoma pathways were enriched in the patients with and without LI, respectively. Using the top five prognostic marker genes, we generated survival scores that could be used to predict the 5-year survival of patients with OC without LI.</P><P><B>Conclusion</B></P><P>The DEGs identified in this study could be used to elucidate the mechanism of tumor progression and to guide the prognosis and treatment of patients with serous OC but without LI.</P>
Yang, San-Duk,Park, Hyun-Seok Korea Genome Organization 2022 Genomics & informatics Vol.20 No.1
A major issue in the use of immune checkpoint inhibitors is their lack of efficacy in many patients. Previous studies have reported that the T cell inflamed signature can help predict the response to immunotherapy. Thus, many studies have investigated mechanisms of immunotherapy resistance by defining the tumor microenvironment based on T cell inflamed and non-T cell inflamed subsets. Although methods of calculating T cell inflamed subsets have been developed, valid screening tools for distinguishing T cell inflamed from non-T cell inflamed subsets using gene expression data are still needed, since general researchers who are unfamiliar with the details of the equations can experience difficulties using extant scoring formulas to conduct analyses. Thus, we introduce TcellInflamedDetector, an R package for distinguishing T cell inflamed from non-T cell inflamed samples using cancer gene expression data via bulk RNA sequencing.
San, Boi Hoa,Kim, Sungsu,Moh, Sang Hyun,Lee, Hyunjoo,Jung, Duk‐,Young,Kim, Kyeong Kyu WILEY‐VCH Verlag 2011 Angewandte Chemie Vol.123 No.50
<P><B>Außen Bio, innen Nano</B>: Durch Einkapselung von Platin‐Nanopartikeln in einer bakteriellen Aminopeptidase resultiert ein neuartiges Hybridkonstrukt, das Platin‐katalysierte Hydrierung und Peptidase‐katalysierte Hydrolyse zu Mehrstufensynthesen kombinieren kann (siehe Bild). Die Übertragung dieses bioanorganischen Einkapselungskonzepts auf zahlreiche Enzyme und anorganische Materialien kann zu neuartigen multifunktionalen Materialien führen.</P>
3,4-Dideoxyglucosone-3-Ene Induces Apoptosis in Human Peritoneal Mesothelial Cells
Lee, Duk-Hyun,Choi, Soon-Youn,Ryu, Hye-Myung,Kim, Chan-Duck,Park, Sun-Hee,Chung, Ho-Young,Kim, In-San,Kim, Yong-Lim SAGE Publications 2009 Peritoneal dialysis international Vol.29 No.1
<B>Objective</B><P> Glucose degradation products (GDPs) are formed during heat sterilization and storage of peritoneal dialysis (PD) fluids. 3,4-dideoxyglucosone-3-ene (3,4-DGE) has been identified as the most bioreactive GDP. 3,4-DGE induces apoptosis in leukocytes and renal tubular epithelial cells. Our aim was to evaluate the apoptotic effects of 3,4-DGE on human peritoneal mesothelial cells (HPMCs). </P><B>Methods</B><P> Primary cultured HPMCs were treated with 25 or 50 μmol/L 3,4-DGE. MTT assay was used to determine cell viability. Apoptosis was measured using TUNEL assay and flow cytometry. Expressions of procaspase-3, Bax, and Bcl-2 were estimated by Western blot. Activity of caspase-3 was measured and the effect of the caspase inhibitor zVAD-fmk (Z-Val-Ala-DL-Asp-fluoromethylketone) was evaluated by TUNEL assay. </P><B>Results</B><P> 3,4-DGE treatment accelerated cell death in HPMCs in a dose- and time-dependent manner. Treatment with 3,4-DGE (25 and 50 μmol/L) significantly increased apoptosis compared to control ( p @@<@@ 0.05 and p @@<@@ 0.01 respectively) by TUNEL assay. Flow cytometry showed treatment with 50 μmol/L 3,4-DGE significantly increased apoptosis compared to control ( p @@<@@ 0.05). Decreased expression of procaspase-3 and increased activity of caspase-3 were observed in the presence of 50 μmol/L 3,4-DGE compared to control and 25 μmol/L 3,4-DGE ( p @@<@@ 0.05). 3,4-DGE-induced HPMC apoptosis was decreased after pretreatment with the pan-caspase inhibitor zVAD-fmk in the 50 μmol/L 3,4-DGE-treated group ( p @@<@@ 0.001). The ratio of Bcl-2 to Bax expression was decreased in the 25 μmol/L and the 50 μmol/L 3,4-DGE-treated groups compared to control ( p @@<@@ 0.05). </P><B>Conclusions</B><P> 3,4-DGE promotes apoptosis in HPMCs by a caspase-related mechanism. </P>