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Saminathan Subburaj,하혜정,진용태,전용삼,Luhua Tu,김진백,강시용,이긍주 한국식물학회 2017 Journal of Plant Biology Vol.60 No.2
MicroRNAs (miRNAs) are endogenous small non-coding RNAs that regulate gene expression at posttranscriptional level. They might regulate tolerance to abiotic stress. However, this has not been studied in Tradescantia (BNL clone 3340), an important bio-indicator for measuring the effect of radiation. Up to date, gamma irradiation (γ-IR) responsive miRNAs and their expressions patterns in plants in response to γ-IR stress have not been reported yet. Therefore, putative γ-IR responsive miRNAs from a cDNA library of Tradescantia after radiation stress were predicted in this study by using comprehensive genomic approaches. Here, we identified 37 miRNAs belonged to 36 different miRNA families. The quantitative real-time polymerase chain reaction (qRT-PCR) analysis of five randomly selected miRNAs were confirmed that their responsiveness to γ-IR stress. Target prediction revealed that 37 miRNAs targeted 149 genes involved in stress tolerance regulation, light response, redox systems, signaling pathways, DNA repair, and transcription factors. A coordinated reverse expression changes between miRNAs and their target genes further validated that these predicted miRNAs could be γ-IR responsive and likely to be directly involved in stress regulatory networks of Tradescantia. Altogether, these results will provide novel insights to the understanding of molecular mechanisms of miRNAs involved in plant response to γ-IR.
Targeted Genome Editing, an Alternative Tool for Trait Improvement in Horticultural Crops
Saminathan Subburaj,Luhua Tu,진용태,배상수,서필준,정유진,이긍주 한국원예학회 2016 Horticulture, Environment, and Biotechnology Vol.57 No.6
Improving crops through plant breeding, an important approach for sustainable agriculture, has been utilized to increase the yield and quality of foods and other biomaterials for human use. Crops, including cereals, vegetables, ornamental flowers, fruits, and trees, have long been cultivated to produce high-quality products for human consumption. Conventional breeding technologies, such as natural cross-hybridization, mutation induction through physical or chemical mutagenesis, and modern transgenic tools are often used to enhance crop production. However, these breeding methods are sometimes laborious and complicated, especially when attempting to improve desired traits without inducing pleiotropic effects. Recently, targeted genome editing (TGE) technology using engineered nucleases, including meganucleases, zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and clustered regularly interspaced short palindromic repeat (CRISPR) nucleases, has been used to improve the traits of economically important plants. TGE has emerged as a novel plant-breeding tool that represents an alternative approach to classical breeding, but with higher mutagenic efficiency. Here, we briefly describe the basic principles of TGE and the types of engineered nucleases utilized, along with their advantages and disadvantages. We also discuss their potential use to improve the traits of horticultural crops through genome engineering.
Saminathan Subburaj,김용휘,이상협,김경남,서미정,김기준,이긍주 한국식물생명공학회 2016 Plant biotechnology reports Vol.10 No.3
MicroRNAs (miRNAs) are a class of small noncoding, single-stranded RNA sequences that regulate gene expression at the post-transcriptional level and also reported to function in stress responses, but their role has not been studied in Camelina (Camelina sativa L.), an emerging oil crop. In this study, we predicted conserved as well as putative novel miRNAs from a Camelina drought stress cDNA library using comprehensive genomic approaches. Based on the sequence homology, we predicted 145 miRNAs, of which 61 were conserved, and 84 putative novel miRNAs were found to belong to 26 and 72 different miRNA families, respectively. In silico expression analysis indicated that 20 miRNAs were really expressed in Camelina genome, and several of them have tissue-specific expression character. We found that the 60 putative novel miRNA families target 117 genes. Most of the miRNA targets were predicted to genes including that regulate stress response, transcription factors, and fatty acid and lipid metabolism-related genes. Expression patterns of 6 randomly selected miRNAs under drought stress were validated by real-time quantitative polymerase chain reaction analysis. Coordinated expression changes between 6 randomly selected miRNAs and their target genes, suggested that the predicted miRNAs could be drought-responsive and that they would likely be directly involved in stress regulatory networks of Camelina. These results indicate that, in C. sativa, under drought stress, a large number of new miRNAs could be discovered, and the predicted stress-responsive miRNAs and their target transcripts will serve as valuable resources for future studies.
Norm estimates and univalence criteria for meromorphic functions
Saminathan Ponnusamy,Toshiyuki Sugawa 대한수학회 2008 대한수학회지 Vol.45 No.6
Norm estimates of the pre-Schwarzian derivatives are given for meromorphic functions in the outside of the unit circle. We deduce several univalence criteria for meromorphic functions from those estimates. Norm estimates of the pre-Schwarzian derivatives are given for meromorphic functions in the outside of the unit circle. We deduce several univalence criteria for meromorphic functions from those estimates.
NORM ESTIMATES AND UNIVALENCE CRITERIA FOR MEROMORPHIC FUNCTIONS
Ponnusamy, Saminathan,Sugawa, Toshiyuki Korean Mathematical Society 2008 대한수학회지 Vol.45 No.6
Norm estimates of the pre-Schwarzian derivatives are given for meromorphic functions in the outside of the unit circle. We deduce several univalence criteria for meromorphic functions from those estimates.