Cissus sicyoides: Analysis of Glycemic Control in Diabetic Rats Through Biomarkers

Salgado, Jocelem Mastrodi,Mansi, Debora Niero,Gagliardi, Antonio The Korean Society of Food Science and Nutrition 2009 Journal of medicinal food Vol.12 No.4

Diabetes is a chronic degenerative disease with no cure, is found in millions of people worldwide, and can cause life-threatening complications at any age. The plant Cissus sicyoides L. is a runner plant found abundantly in Brazil, especially in the Amazon. Its therapeutic properties are widely used in popular medicine as a diuretic, anti-influenza, antiinflammatory, anticonvulsion, and hypoglycemic agent. The objective of this study was to analyze the effects of aqueous extracts from the leaves and stem of C. sicyoides L., administered for 60 days, for the control of glycemia in alloxan (monohydrate)-induced diabetic rats, monitored by biomarkers. Data obtained in this study confirmed that C. sicyoides has a hypoglycemic effect on diabetic rats. Administration of its aqueous extracts promoted a 45% decrease in glucose levels after 60 days of administration. Furthermore, indices of hepatic glycogen, blood glucose, C-reactive peptide, and fructosamine were found to be efficient biomarkers to monitor diabetes in rats.

Kansei and Design: A Systematic Mapping Review

( Sánchez-salgado¸ A. ),( Lee¸ S. H. ),( Sanabria-z¸ J. ) 한국감성과학회 2021 추계학술대회 Vol.2021 No.0

Notwithstanding the significant influence the application of Kansei has had on the field of design in recent decades, there is no record of analyses of its recent modes of implementation, limiting the transfer of knowledge for the emergence of future projects. In this regard, a Systematic Mapping Review was carried out to identify the current status of research related to Kansei and Design. Publications in Scopus and Web of Science databases from the last six years were analyzed considering the number of publications, location, and research area, among others. Findings show that while Asia continues to lead in the number of publications, diversity exists in the nationality of the authors. Fields of application, include product design, and aesthetic design along with an ongoing interest within technology areas. In general terms, it became evident that the interest in Kansei has not only remained current, but has increased and has been expanding geographically. Moreover, its application in the Design area has become so broad that it ranges from Emotional Design to Human-Robot Interaction Design. It is envisaged that the results of this study will contribute to identifying future areas of research at the convergence of kansei and design fields.

Salinity as a Regulator of DMSP Degradation in Ruegeria pomeroyi DSS-3

Paula Salgado,Ronald Kiene,William Wiebe,Catarina Magalhães 한국미생물학회 2014 The journal of microbiology Vol.52 No.11

Dimethylsulfoniopropionate (DMSP) is an important carbonand sulfur source to marine bacterial communities andthe main precursor of dimethylsulfide (DMS), a gas that influencesatmospheric chemistry and potentially the globalclimate. In nature, bacterial DMSP catabolism can yield differentproportions of DMS and methanethiol (MeSH), butrelatively little is known about the factors controlling thepathways of bacterial degradation that select between theirformation (cleavage vs. demethiolation). In this study, wecarried out experiments to evaluate the influence of salinityon the routes of DMSP catabolism in Ruegeria pomeroyiDSS-3. We monitored DMS and MeSH accumulation in cellsuspensions grown in a range of salinities (10, 20, 30 ppt)and with different DMSP amendments (0, 50, 500 μM). Significantly higher concentrations of DMS accumulated inlow salinity treatments (10 ppt; P < 0.001), in both MarineBasal Medium (MBM) and half-strength Yeast Tryptone SeaSalts (½ YTSS) media. Results showed a 47.1% and 87.5%decrease of DMS accumulation, from salinity 10 to 20 ppt,in MBM and ½ YTSS media, respectively. On the otherhand, MeSH showed enhanced accumulations at highersalinities (20, 30 ppt), with a 90.6% increase of MeSH accumulationfrom the 20 ppt to the 30 ppt salinity treatments. Our results with R. pomeroyi DSS-3 in culture are in agreementwith previous results from estuarine sediments anddemonstrate that salinity can modulate selection of the DMSPenzymatic degradation routes, with a consequent potentialimpact on DMS and MeSH liberation into the atmosphere.

Kansei and Design: A Systematic Mapping Review

( Sánchez-salgado¸ A. ),( Lee S. H. ),( Sanabria-z¸ J. ) 한국감성과학회 2021 한국감성과학회 국제학술대회(ICES) Vol.2021 No.-

Notwithstanding the significant influence the application of Kansei has had on the field of design in recent decades, there is no record of analyses of its recent modes of implementation, limiting the transfer of knowledge for the emergence of future projects. In this regard, a Systematic Mapping Review was carried out to identify the current status of research related to Kansei and Design. Publications in Scopus and Web of Science databases from the last six years were analyzed considering the number of publications, location, and research area, among others. Findings show that while Asia continues to lead in the number of publications, diversity exists in the nationality of the authors. Fields of application, include product design, and aesthetic design along with an ongoing interest within technology areas. In general terms, it became evident that the interest in Kansei has not only remained current, but has increased and has been expanding geographically. Moreover, its application in the Design area has become so broad that it ranges from Emotional Design to Human-Robot Interaction Design. It is envisaged that the results of this study will contribute to identifying future areas of research at the convergence of kansei and design fields.

A novel low-cost method for Mycobacterium avium subsp. paratuberculosis DNA extraction from an automated broth culture system for real-time PCR analysis

Miguel Salgado,Cristobal Verdugo,Cord Heuer,Pedro Castillo,Patricia Zamorano 대한수의학회 2014 Journal of Veterinary Science Vol.15 No.2

PCR is a highly accurate technique for confirming thepresence of Mycobacterium avium subsp. paratuberculosis(Map) in broth culture. In this study, a simple, efficient, andlow-cost method of harvesting DNA from Map cultured inliquid medium was developed. The proposed protocol(Universidad Austral de Chile [UACH]) was evaluated bycomparing its performance to that of two traditionaltechniques (a QIAamp DNA Stool Mini Kit andcethyltrimethylammonium bromide [CTAB] method). Theresults were statistically assessed by agreement analysis forwhich differences in the number of cycles to positive (CP)were compared by Student’s t-test for paired samples andregression analysis. Twelve out of 104 fecal pools culturedwere positive. The final PCR results for 11 samples analyzedwith the QIAamp and UACH methods or ones examinedwith the QIAamp and CTAB methods were in agreement. Complete (100%) agreement was observed between datafrom the CTAB and UACH methods. CP values for theUACH and CTAB techniques were not significantlydifferent, while the UACH method yielded significantlylower CP values compared to the QIAamp kit. The proposedextraction method combines reliability and efficiency withsimplicity and lower cost.

The Role of Black Rice (Oryza sativa L.) in the Control of Hypercholesterolemia in Rats

Jocelem Mastrodi Salgado,Anderson Giovanni Candido de Oliveira,Debora Niero Mansi,Carlos M. Donado-Pestana,Candido Ricardo Bastos,Fernanda Klein Marcondes 한국식품영양과학회 2010 Journal of medicinal food Vol.13 No.6

Cardiovascular disease is a serious public health problem; it is the first “cause of death” in Brazil and in developed countries. Thus, it is essential to search for alternative sources such as some functional foods to prevent and control the risks of this disease. The purpose of this study was to evaluate the lipidemic parameters in hypercholesterolemic rats fed diets containing black rice variety IAC 600 or unrefined rice. Adult male Wistar rats (Rattus norvegicus var. albinos) were used, weighing about 200–220g. The animals were divided into four groups: the first received a control casein diet, the second received hypercholesterolemic diet, and the other two groups, after induction of hypercholesterolemia, received the test diets, the first containing 20% black rice and the second 20% unrefined, for 30 days. It was observed that diet containing black rice reduced the level of plasma cholesterol, triglycerides, and low-density lipoprotein. For high-density lipoprotein values, the diet that provided an increase in the levels was the black rice. The diet containing black rice was more effective in controlling the lipidemia in rats compared with the whole rice diet.

Conjugated Linoleic Acid Combined with Physical Activity Reduces Body Fat Accumulation But Does Not Modify Lean Body Mass in Male and Female Wistar Rats

Jocelem Mastrodi Salgado,Taˆnia Rachel Baroni Ferreira,Carlos M. Donado-Pestana,Omer Cavalcanti de Almeida,Aline Mouro Ribeiro das Neves,De´bora Niero Mansi,Carlos Tadeu dos Santos Dias 한국식품영양과학회 2012 Journal of medicinal food Vol.15 No.4

Several biological and clinical studies have suggested that conjugated linoleic acid (CLA) prevents body fat accumulation and increases lean body mass. CLA is available as a concentrated dietary supplement and is purported to provide the aforementioned benefits for people who perform physical activity. This study was conducted to evaluate the effect of a CLA-supplemented diet combined with physical activity on the body composition of Wistar rats. Two groups of Wistar rats of both sexes, between 45 and 60 days old, were fed a diet containing 5.5% soybean oil (control group) or a CLA-supplemented diet (0.5% CLA and 5.0% soybean oil) (test group). Half the rats in both groups were assigned to exercise by running on a treadmill. The biochemical and anatomical body compositions were analyzed. In both groups, CLA had no effect on the dietary consumption or the weight of the liver, heart, and lungs. However, it did influence the overall weight gain of exercised male rats and the chemical and anatomical body composition in exercised and sedentary rats of both sexes. The results confirm that a CLA-supplemented diet with and without physical activity reduced body fat accumulation in rats of both sexes. However, there is no evidence of an increase in the lean body mass of the exercised rats.

Cissus sicyoides: Analysis of Glycemic Control in Diabetic Rats Through Biomarkers

Jocelem Mastrodi Salgado,Débora Niero Mansi,Antonio Gagliardi 한국식품영양과학회 2009 Journal of medicinal food Vol.12 No.4

Diabetes is a chronic degenerative disease with no cure, is found in millions of people worldwide, and can cause life-threatening complications at any age. The plant Cissus sicyoides L. is a runner plant found abundantly in Brazil, especially in the Amazon. Its therapeutic properties are widely used in popular medicine as a diuretic, anti-influenza, anti-inflammatory, anticonvulsion, and hypoglycemic agent. The objective of this study was to analyze the effects of aqueous extracts from the leaves and stem of C. sicyoides L., administered for 60 days, for the control of glycemia in alloxan (monohydrate)-induced diabetic rats, monitored by biomarkers. Data obtained in this study confirmed that C. sicyoides has a hypoglycemic effect on diabetic rats. Administration of its aqueous extracts promoted a 45% decrease in glucose levels after 60 days of administration. Furthermore, indices of hepatic glycogen, blood glucose, C-reactive peptide, and fructosamine were found to be efficient biomarkers to monitor diabetes in rats.

Experimental Tracheal Replacement: Angiogenesis and Null Apoptosis Promote Stenosis

J. Alfredo Santibáñez-Salgado,Avelina Sotres-Vega,Miguel O. Gaxiola-Gaxiola,,Jaime Villalba-Caloca,Karen Bobadilla Lozoya,Joaquín A. Zúñiga-Ramos 대한흉부외과학회 2021 Journal of Chest Surgery (J Chest Surg) Vol.54 No.3

Background: Tracheal replacement is a challenge for thoracic surgeons due to stenosis in the trachea-prosthesis anastomosis. We propose that stenosis occurs due to fibrosis as a result of an abnormal healing process, characterized by an increased expression of wound healing growth factors (vascular endothelial growth factor [VEGF], survivin, and CD31), which promote angiogenesis and decrease apoptosis. We analyzed the immunoreactivity of VEGF, survivin, CD31, and caspase-3 in the development of fibrotic stenosis in prosthetic tracheal replacement. Methods: Fourteen dogs were operated on: group I (n=7) received a 6-ring cervical tra- cheal segment autograft, while in group II (n=7), a 6-ring segment of the cervical trachea was resected and tracheal continuity was restored with a Dacron prosthesis. The follow-up was 3 months. Immunoreactivity studies for VEGF, survivin, CD31, and caspase-3 were per- formed. A statistical analysis was done using the Wilcoxon signed rank test. Results: Four animals in group I were euthanized on the 10th postoperative day due to autograft necrosis. Three animals completed the study without anastomotic stenosis. Moderate expression of VEGF (p=0.038), survivin (p=0.038), and CD31 (p=0.038) was found. All group II animals developed stenosis in the trachea-prosthesis anastomotic sites. Micros- copy showed abundant collagen and neovascularization vessels. Statistically significant immunoreactive expression of VEGF (p=0.015), survivin (p=0.017), and CD31 (p=0.011) was observed. No expression of caspase-3 was found. Conclusion: We found a strong correlation between fibrosis in trachea-prosthesis anas- tomoses and excessive angiogenesis, moderate to intense VEGF, CD31, and survivin ex- pression, and null apoptotic activity. These factors led to uncontrolled collagen production.

HOW DO PEOPLE LOOK AT ONLINE FASHION? AN EXPLORATORY STUDY OF ATTENTION TO DESIGN ELEMENTS IN FASHION WEBSITES

Marta Blázquez,Carlos Velasco,Alejandro Salgado-Montejo,Charles Spence 글로벌지식마케팅경영학회(GFMC) 2015 Global Fashion Management Conference Vol.2015 No.06