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구강 편평 상� 암종의 전이시 발현이 증가된 유전자들의 검색
윤도준,육종인,박상욱 關東大學校 醫科大學 醫科學硏究所 1999 關東醫大學術誌 Vol.3 No.1
We compared the gene expressions in primary and metastic tumor cells derived from two oral squamous cell carcinoma patients. cDNA was synthesized from RNA of primary and metastic tumor cells and amplified with arbitrary primers by polymerase chain reaction. cDNA of increased and decreased gene expression on 6% denaturing polycrylamide gel eletrophoresis was confirmed by dot blot hybridization. We identified 9 cDNAs of increased gene expression and 3 cDNAs of decreased in both merastatic tumor cells. But the gene expression of others was increased or decreased in one of two metastatic tumor cells .These result imply that the pattern of changes of gene expression in metastasis was different in different patients.
Effects of Opuntia humifusa Seed Powder on Serum Lipid Profile in Ovariectomized Rats
Sahng-Wook Hahm ,Jieun Park,Yong-Suk Son 한국식품영양과학회 2011 Preventive Nutrition and Food Science Vol.16 No.3
Opuntia humifusa contains high levels of antioxidants including vitamin C, flavonoids and polyphenols, which may provide beneficial effects such as hypolipidemic activity and the reduction of atherosclerosis in postmenopausal women. This study was conducted to determine if the intake of O. humifusa seeds powder (OHS) regulates lipid concentrations, glutamate-oxaloacetate transaminase (GOT), and glutamate-pyruvate transaminase (GPT) in the serum of ovariectomized rats. Sprague-Dawley female rats were randomly assigned to either a sham-operated group (Sham) or one of the following four ovariectomy (OVX) subgroups: OVX with vehicle (OVX), OVX with 100, 200, and 500 mg/kg/day OHS (OHS100, OHS200, OHS500). Daily oral administration of OHS was initiated one week after ovariectomy and continued for seven weeks. Upon completion of treatments, organs were weighed and GOT, GPT, total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C) and high-density lipoprotein cholesterol (HDL-C) levels were determined enzymatically. No significant differences in feed intake and organ index were observed among the groups. Significant decreases in GPT, TC and LDL-C (p<0.05) were observed in all of the OHS groups (OHS100, 200 and 500), while no significant changes in HDL-C were observed. In addition, the OHS200 and OHS500 treatment groups exhibited a lower level of serum GOT compared to the OVX group. These results indicate that supplementation with O. humifusa seeds could induce favorable changes in serum lipoprotein and lipid profiles, which frequently worsen with inadequate estrogen availability.