Novel pan-lineage VP1 specific degenerate primers for precise genetic characterization of serotype O foot and mouth disease virus circulating in India

Sagar Ashok Khulape 대한수의학회 2023 Journal of Veterinary Science Vol.24 No.3

Analysis of the VP1 gene sequence of the foot and mouth disease virus (FMDV) is critical to understanding viral evolution and disease epidemiology. A standard set of primers have been used for the detection and sequence analysis of the VP1 gene of FMDV directly from suspected clinical samples with limited success. The study validated VP1-specific degenerate primer-based reverse transcription polymerase chain reaction (RT-PCR) for the qualitative detection and sequencing of serotype O FMDV lineages circulating in India. The novel degenerate primer-based RT-PCR amplifying the VP1 gene can circumvent the genetic heterogeneity observed in viruses after cell culture adaptation and facilitate precise viral gene sequence analysis from clinical samples.

Magnetodielectric Effect in Microwave-Sintered Ba<SUB>0.8</SUB>Sr<SUB>0.2</SUB>TiO₃ and Co<SUB>0.9</SUB>Ni<SUB>0.1</SUB>Fe₂O₄ Composite

Sagar M. Mane,Sachin A. Pawar,Dipali S. Patil,Seong Hun Lee,Jae Cheol Shin 한국진공학회(ASCT) 2020 Applied Science and Convergence Technology Vol.29 No.2

Composites constructed by combining ferroelectric and ferromagnetic materials are of significant interest owing to their multifunctional features and potential applications in multifunctional devices. Herein, the magnetodielectric effect of a composite containing ferroelectric Ba0.8Sr0.2TiO₃ (BST) and ferrite Co0.9Ni0.1Fe₂O₄ (CNFO) phases is investigated. This ferroelectric and ferrite composite is synthesized using the co-precipitation method followed by microwave sintering. The tetragonal crystal structure of BST and cubic structure of CNFO are confirmed using X-ray diffraction. All composite samples are characterized using nanoparticle tracking analysis. The measurements of dielectric constant (room temperature) and loss tangent as a function of frequency show an increase in the dielectric constant with an increase in the CNFO phase. The highest value of magnetodielectric coefficient, -40.13 %, is observed for the 60BST-40CNFO composite at 100 Hz, which decreases with an increase in the applied frequency.

Deterministic Seismic Hazard Map Using Region-Specific GMPEs of Maharashtra State, India

Sagar Dhole,Sachin Bakre 대한토목학회 2024 KSCE Journal of Civil Engineering Vol.28 No.2

Maharashtra stands out as a crucial state in India, demonstrating significant progress in infrastructural development and industrialization. Several prominent cities, including Mumbai, Pune, Nagpur, etc., are significantly contributing to the Indian economy. Considering the importance of the state, a deterministic seismic hazard analysis is executed to reduce the damages to critical and important structures and fatalities caused due to earthquakes. Past earthquakes data are collected within and around the state to prepare a homogenised earthquake catalogue. Seven seismic zones are prepared using K- mean cluster analysis. Independent earthquake events i.e., mainshocks are identified using four renowned declustering methods. Additionally, with the help of mainshocks from each zone, the maximum observed earthquake magnitude () and positive correction factor (Δ) are estimated. By superimposing all the mainshocks (after adding Δ) onto the fault map, the maximum observed possible earthquake magnitude of all faults () are assigned to each fault. value is used to estimate surface rupture length (RLD) and consecutively, the maximum magnitude (MMax) from fault sources are estimated. Three region-specific ground motion prediction equations (GMPEs) are adopted in the logic trees assigning a proper weightage to each GMPE. A seismic hazard contour maps are prepared at bedrock level, C, and D-type soil sites for Maharashtra. In the western part of the study area, the maximum PGA value is found to be 0.58 g, 0.70 g, and 0.33 g at bedrock level, C, and D-type sites, respectively.

Evaluation of ciprofloxacin and metronidazole encapsulated biomimetic nanomatrix gel on Enterococcus faecalis and Treponema denticola

Sagar N Kaushik,Jessica Scoffield,Adinarayana Andukuri,Grant C Alexander,Taneidra Walker,김석곤,최성철,Brigitta C Brott,Paul D Eleazer,이진용,Hui Wu,Noel K Childers,Ho-Wook Jun,박재홍,Kyounga Cheon 한국생체재료학회 2015 생체재료학회지 Vol.19 No.2

Background: A triple antibiotic mixture (ciprofloxacin; CF, metronidazole; MN, and minocycline; MC) has been used for dental root canal medicaments in pulp regeneration therapy. However, tooth discolorations, cervical root fractures, and inadequate pulp-dentin formation have been reported due to the triple antibiotic regimen. Therefore, an antibiotic encapsulated biomimetic nanomatrix gel was developed to minimize the clinical limitations and maximize a natural healing process in root canal infections. In this study, minimal bacterial concentrations (MBC) of the selected antibiotics (CF and MN) were tested in 14 representative endodontic bacterial species. Then MBC of each CF and MN were separately encapsulated within the injectable self-assembled biomimetic nanomatrix gel to evaluate antibacterial level on Enterococcus faecalis and Treponema denticola. Results: Antibiotic concentrations lower than 0.2 μg/mL of CF and MN demonstrated antibacterial activity on the 14 endodontic species. Furthermore, 6 different concentrations of CF and MN separately encapsulated with the injectable self-assembled biomimetic nanomatrix gel demonstrated antibacterial activity on Enterococcus faecalis and Treponema denticola at the lowest tested concentration of 0.0625 μg/mL. Conclusions: These results suggest that each CF and MN encapsulated within the injectable self-assembled biomimetic nanomatrix gel demonstrated antibacterial effects, which could be effective for the root canal disinfection while eliminating MC. In the long term, the antibiotic encapsulated injectable self-assembled biomimetic nanomatrix gel can provide a multifunctional antibiotic delivery method with potential root regeneration. Further studies are currently underway to evaluate the effects of combined CF and MN encapsulated within the injectable self-assembled biomimetic nanomatrix gel on clinical samples.

Biomimetic microenvironments for regenerative endodontics

Sagar N Kaushik,Bogeun Kim,Alexander M. Cruz Walma,Sung Chul Choi,Hui Wu,Jeremy J. Mao,Ho-Wook Jun,Kyounga Cheon 한국생체재료학회 2016 생체재료학회지 Vol.20 No.2

Regenerative endodontics has been proposed to replace damaged and underdeveloped tooth structures with normal pulp-dentin tissue by providing a natural extracellular matrix (ECM) mimicking environment; stem cells, signaling molecules, and scaffolds. In addition, clinical success of the regenerative endodontic treatments can be evidenced by absence of signs and symptoms; no bony pathology, a disinfected pulp, and the maturation of root dentin in length and thickness. In spite of the various approaches of regenerative endodontics, there are several major challenges that remain to be improved: a) the endodontic root canal is a strong harbor of the endodontic bacterial biofilm and the fundamental etiologic factors of recurrent endodontic diseases, (b) tooth discolorations are caused by antibiotics and filling materials, (c) cervical root fractures are caused by endodontic medicaments, (d) pulp tissue is not vascularized nor innervated, and (e) the dentin matrix is not developed with adequate root thickness and length. Generally, current clinical protocols and recent studies have shown a limited success of the pulp-dentin tissue regeneration. Throughout the various approaches, the construction of biomimetic microenvironments of pulp-dentin tissue is a key concept of the tissue engineering based regenerative endodontics. The biomimetic microenvironments are composed of a synthetic nano-scaled polymeric fiber structure that mimics native pulp ECM and functions as a scaffold of the pulp-dentin tissue complex. They will provide a framework of the pulp ECM, can deliver selective bioactive molecules, and may recruit pluripotent stem cells from the vicinity of the pulp apex. The polymeric nanofibers are produced by methods of self-assembly, electrospinning, and phase separation. In order to be applied to biomedical use, the polymeric nanofibers require biocompatibility, stability, and biodegradability. Therefore, this review focuses on the development and application of the biomimetic microenvironments of pulp-dentin tissue among the current regenerative endodontics.

Impact on survival with adjuvant radiotherapy for clear cell, mucinous, and endometriod ovarian cancer: the SEER experience from 2004 to 2011

Sagar C. Patel,Jonathan Frandsen,Sudershan Bhatia,David Gaffney 대한부인종양학회 2016 Journal of Gynecologic Oncology Vol.27 No.5

Objective: Evaluate the impact of radiotherapy on cause specific survival (CSS) and overallsurvival (OS) for stage (I–III) clear cell, mucinous, and endometriod ovarian cancer. Methods: We analyzed incidence, survival, and treatments from the Surveillance,Epidemiology, and End Results (SEER) Program from 2004 to 2011 for clear cell, mucinous,and endometriod histologies of the ovary for stages (I–III). We examined CSS and OS for allthree histologies combined and each histology with relation to the use of adjuvant radiationtherapy (RT). Survival analysis was calculated by Kaplan-Meier and log-rank analysis. Results: CSS was higher in individuals not receiving RT at 5 years (81% vs. 74%) and 10 years(74% vs. 65%, p=0.003). OS was higher in individuals not receiving RT at 5 years (76% vs. 73%) and 10 years (64% vs. 59%, p=0.039). Stage III patients receiving RT had a higher OS at5 years (54% vs. 44%) and 10 year intervals (36% vs. 30%, p=0.037). Stage III patients withmucinous histology receiving RT had a higher OS at 5 years (50% vs. 36%) and 10 years (45%vs. 26%, p=0.052). Conclusion: Those receiving RT had a lower CSS and OS at 5 and 10 years. However, subgroupanalysis revealed a benefit of RT in terms of OS for all stage III patients and for stage IIIpatients with mucinous histology.

Estimating shear wave velocities in oil fields: a neural network approach

Sagar Singh,Ali Ismet Kanli 한국지질과학협의회 2016 Geosciences Journal Vol.20 No.2

In this study, we applied the back-propagation Artificial Neural Network (ANN) technique to test the shear-velocity for the two wells from an oil field in southeastern region of Turkey estimated from an empirical relationship. The input to the neural network includes neutron porosity, density, true resistivity, P-wave velocity and gamma-ray logs which are known to affect the shearwave velocity. The correlation between the shear-wave velocity from the empirical relationship and that from the neural network is close to one in both the training and testing stages. Thus, the ANN technique can be used to predict shear-wave velocity from other well log data.

Effect of microstructure and cutting speed on machining behavior of Ti6Al4V alloy

Sagar V. Telrandhe,Ashish K. Saxena,Sushil Mishra 대한기계학회 2017 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.31 No.5

Machining of aerospace and biomedical grade titanium alloys has always been a challenge because of their low conductivity and elastic modulus. Different machining methods and parameters have been adopted for high precision machining of titanium alloys. Machining of titanium alloys can be improved by microstructure optimization. The present study focuses on the effect of microstructure on machinability of Ti6Al4V alloys at different cutting speeds. Samples were subjected to different annealing conditions resulting in different grain sizes and local micro-strains (misorientation). Cutting forces were significantly reduced after annealing; consequently, sub-surface residual stresses were reduced. Deformation twinning was also observed on samples annealed at a higher temperature due to larger grain size. Initial strain free grains and deformation twinning during machining reduces the cutting force at higher cutting speed.

Development of Novel Multifunctional Bioactive Nanotherapeutics for the Regeneration of Infected Hard Tissue Defects

Sagar Varsha,JunHee Lee,Jeongeun Hyun,HaeWon Kim,HaeHyoung Lee,JungHwan Lee 대한치과재료학회 2022 대한치과기재학회 학술대회 Vol.2022 No.4

Antibiofouling effects of honokiol and magnolol on Acinetobacter baumannii

Sagar Kiran KHADKE,Jin-Hyung LEE,Jintae LEE 한국생물공학회 2019 한국생물공학회 학술대회 Vol.2019 No.4