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Feeding-based RNA Interference of RSV Genes in RSV-viruliferous Laodelphax striatellus
Saes Byeol An,Ying Fang,Jae Young Choi,Seok-Hee Lee,Jong Hoon Kim,Shin Sang Woon,Woo Jin Kim,Yeon Ho Je 한국응용곤충학회 2014 한국응용곤충학회 학술대회논문집 Vol.2014 No.10
Rice stripe virus (RSV) is one of the serious plant pathogenic viruses for rice transmitted by small brown planthopper, SBPH, Laodalphax striatellus. RNA interference (RNAi) is an universal gene-knockdown mechanism in eukaryotic organisms which includes insects,and has been considered as an alternative strategy to control insect pests. Hence, we applied this technique to interfere the translation of target RNA genes to knockdown the virus gene on RSV-viruliferous L. striatellus. Three out of seven RSV genes, RdRp, NS3, and NCP were used as target genes and each dsRNA targeting the viral genes were delivered to the insects indirectly through the rice leaves by irrigation. As a result, not only the relative expression level of target genes decreased but also those of non-target genes and the replication of RSV genome as well. In summary, leaf-mediated dsRNA feeding methods would be useful in the knockdown of target genes on piercing-sucking insects. The genes used in this experiment can be utilized for the development of pest-resistant transgenic plants based on RNAi.
Saes Byeol An,Jae Young Choi,Qin Liu,Song Eun Kim,Seok Hee Lee,Woo Jin Kim,Yeon Ho Je 한국응용곤충학회 2013 한국응용곤충학회 학술대회논문집 Vol.2013 No.10
Rice stripe virus disease (RSVD), one of the most serious disease of rice is mediated through the sucking by small brown planthopper, Laodalphax striatellus. So far, the studies have been mainly focused on the interaction between the host plant and the virus. In this study, for better comprehension of the interactions among the host plant, vector insect and plant-pathogenic virus, we investigated transcriptome of the vector insect and the differences between viruliferous and naïve L.striatellus. For this, naïve L. striatellus were collected from non-infected rice field and 50 L.striatellus of them were fed RSV-infected rice for 5 days. With the RSV-viruliferous and the naïve insects, we conducted Illumina RNA sequencing (Hiseq 2000) and obtained 175,243,488 and 146,031,348 reads from viruliferous and naïve L.striatellus, respectively. These reads were assembled into contigs and two transcriptome databases were generated. The transcriptome of naïve and RSV-viruliferous L. striatellus were campared to figure out up-regulated or down-regulated genes. These RSV-dependently regulated genes may have important function in the behavior of planthoppers or the transmission of RSV.
Saes Byeol An,Jae Young Choi,Seok Hee Lee,Ying Fang,Jong Hoon Kim,Seung Hee Lee,Shin Sang Woon,Woo Jin Kim,Yeon Ho Je 한국응용곤충학회 2014 한국응용곤충학회 학술대회논문집 Vol.2014 No.04
Rice stripe virus (RSV) is one of the serious plant pathogenic viruses for rice and mediated by small brown planthopper, Laodalphax striatellus. So far, the studies have been mainly focused on the interaction between the host plant and the virus. In this study, for better comprehension of the interactions among Rice stripe virus, rice and small brown planthopper, transcriptomes of the RSV-viruliferous (RVLS) and non-viruliferous L. striatellus (NVLS) were comparatively analysed. For this, non-viruliferous L. striatellus were collected from non-infected rice field and fed RSV-infected rice for 5 days. With the RNAs prepared from the RSV-viruliferous and the non-viruliferous small brown planthoppers, we conducted Illumina RNA sequencing (Hiseq 2000) and then two transcriptome databases were generated from RVLS and NVLS, respectively. The transcriptome of RVLS and NVLS were campared to figure out how the gene expression of the insects affected by Rice Stripe Virus. RSV-dependently regulated genes analysed from this study may have important functions in the transmission and replication of RSV.
2015 개정 교육과정 고등학교 영어, 영어Ⅰ, 영어Ⅱ교과서 문화내용 비교 분석
김샛별(Saes-Byul Kim),신동진(Dong-Jin Shin) 한국영어교과교육학회 2022 영어교과교육 Vol.21 No.2
The purpose of this study is to analyze cultural contents in Korean high school English textbooks in accordance with 2015 Korean National Curriculum. A total of 30 high school English textbooks for English, English I, and English II for 1st, 2nd, and 3rd year students, respectively, were selected and analyzed according to the topics of cultural contents, regional backgrounds of cultural contents, and types of cultural contents. In addition, the Korean cultural contents of the textbooks were examined according to cultural types. The results are as follows. First, among 19 cultural topics, the most common in all years was general education. Second, universal culture occupied more than 65% in all years. Third, all of the textbooks present three cultural types, with behavioral culture showed the highest. Fourth, the Korean cultural contents tend to be focused on material culture. Based on the findings, the study provides several suggestions for cultural learning in English textbooks. First, 19 cultural topics need to be distributed more evenly. Second, reduce universal cultural contents and increase Korean culture so that learners know the value of their culture.
Identification of a major quantitative trait locus for bakanae disease resistance in rice
Yeon-Jae Hur,Saes-Beul Lee,Tae-Heon Kim,Jong-Hee Lee,Dong-Jin Shin,Soo-Kwon Park,Woon-Ha Hwang,Sang-Ik Han,Jun-Hyun Cho,Young-Nam Yoon,Un-Sang Yeo,You-Chun Song,Min-Hee Nam,Dong-Soo Park 한국육종학회 2014 한국육종학회 심포지엄 Vol.2014 No.07
Bakanae disease incidence threat is an increasing trend in the top rice growing countries. Despite it is essential to identify the resistant genes and underlying mechanisms of bakanae disease to develop resistant varieties, there are very limited genetic studies on bakanae disease in rice. The indica rice variety Shingwang was selected as resistant donor to bakanae disease. One hundred sixty nine NILs, YR28297 (BC6F4) generated by five backcrosses of Shingwang with the genetic background of susceptible japonica variety, Ilpum were used for QTL analysis. Rice bakanae disease pathogen, CF283, was mainly used in this study and inoculation and evaluation of bakanae disease was performed with the method of the large-scale screening method developed by Kim et al. (2014). The proportion of healthy plants of Shingwang and Ilpum after inoculation was confirmed using bakanae disease pathogen, CF283. While inoculated Ilpum showed thin and yellowish-green phenotype which is typical symptom of Bakanae disease, Shingwang showed similar healthy phenotype with control plants. A major QTL for resistance against bakanae disease on chromosome 1 was identified using SSR marker, RM9, which explaining 65 % of the total phenotype variation. The major QTL designated as qBK1 and mapped to a 4.4 Mbp region between RM24 (19.30 Mb) and RM11295 (23.72 Mb). The information of qBK1 could be useful for improving rice bakanae disease resistance in marker-assisted breeding.
QTL analysis of the qBK1, a major QTL for bakanae disease resistance in rice
Yeon-Jae Hur,Saes-Beul Lee,Tae-Heon Kim,Jong-Hee Lee,Dong-Jin Shin,Soo-Kwon Park,Woon-Ha Hwang,Sang-Ik Han,Jun-Hyun Cho,Young-Nam Yoon,Un-Sang Yeo,You-Chun Song,Min-Hee Nam,Dong-Soo Park 한국육종학회 2014 한국육종학회 심포지엄 Vol.2014 No.07
Bakanae disease is one of the most serious and oldest problems of rice production, which was first described in 1828 in Japan (Ito and Kimura 1931). This disease may infect rice plants from the pre-emergence stage to the mature stage, with severe infection of rice seeds resulting poor germination or withering (Iqbal et al. 2011). Under favorable environmental conditions, infected plants have the capacity to produce numerous conidia that subsequently infect proximate healthy plants, resulting in major yield loss (Ou 1985). One hundred sixty nine NILs, YR28297 (BC6F4) generated by five backcrosses of Shingwang with the genetic background of susceptible japonica variety, Ilpum were used for QTL analysis. Rice bakanae disease pathogen, CF283, was mainly used in this study and inoculation and evaluation of bakanae disease was performed with the method of the large-scale screening method developed by Kim et al. (2014). A major QTL for resistance against bakanae disease on chromosome 1 was identified using SSR marker, RM9, which explaining 65 % of the total phenotype variation. The major QTL designated as qBK1 and mapped to a 4.4 Mbp region between RM24 (19.30 Mb) and RM11295 (23.72 Mb). The results of this study are expected to provide useful information toward developing resistant rice lines to this detrimental fungal disease.
Comparative Transcriptome Analysis of Queen, Worker, and Larva of Asian Honeybee, Apis cerana
Seok Hee Lee,Sang Woon Shin,Saes Byeol An,Fang Ying,Jong Hoon Kim,Seung Hee Lee,Jae Young Choi,Woo Jin Kim,Yeon Ho Je 한국응용곤충학회 2014 한국응용곤충학회 학술대회논문집 Vol.2014 No.04
The Asian honeybee, Apis cerana, is a native honeybee species in Korea which is important in agriculture for pollination and honey production. For better understanding of the physiology of A. cerana, high-throughput Illumina transcriptome sequencing was performed to analyze the gene expression profiles of queen, worker and larva. A total of 219,799,682 clean reads corresponding to 22.2Gb of nucleotide sequences was obtained from the whole body total RNA samples. The Apis mellifera reference mRNA sequence database was used to measure the gene expression level with Bowtie2 and eXpress software and the Illumina short reads were mapped to 11,459 out of 11,736 A. mellifera reference genes. Total of 9,221 genes with FPKM value greater than 5 of each sample group were subjected to evolutionary genealogy of genes: Non-supervised Orthologous Groups (eggNOG) with BLASTX for gene ontology analysis. The differential gene expression between queen and worker, and worker and larva were analyzed to screen the overexpressed genes in each sample group. in the queen and worker sample group, total of 1,766 genes were differentially expressed with 887 and 879 genes overexpressed over two folds in queen and worker, respectively. In the worker and larva sample group, total of 1,410 genes were differentially expressed with 1,009 and 401 genes overexpressed over two folds in worker and larva, respectively.
Discovery of Plant Antagonists of Insect Juvenile Hormone
Seok-Hee Lee,Ying Fang,Saes-Byeol An,Doo-Sang Park,Hyuk-Hwan Song,Sei-Ryang Oh,Soo-Young Kim,Seonghyun Kim,Namjung Kim,Hyun-Woo Oh,Yeon Ho Je,Sang Woon Shin 한국응용곤충학회 2014 한국응용곤충학회 학술대회논문집 Vol.2014 No.10
Insects impact human health through vector-borne diseases and cause major economic loses through damaging crops and stored agricultural products. Insect-specific growth regulators (IGR) represent attractive control agents because of their safety to the environment and humans. Here, we report identification of plant compounds that are antagonists of the insect-specific juvenile hormone (PJHANs), using the yeast two hybrid system transformed with the mosquito JH receptor as a reporter assay. We show that these compounds act by inhibiting larval growth and reproduction in mosquitoes. We also demonstrate that PJHANs affect the JH receptor, Methoprene-tolerant (Met), by disrupting its complex with CYCLE, formation of which is required for mediating JH action. We isolated five diterpene secondary metabolites with JH antagonist activity from two plants, Lindera erythrocarpa and Solidago serotina. They are effective in causing mortality of mosquito larvae at relatively low LD50 values. Two of these diterprenes affect Met function, leading to reduction in expression of Met target genes and causing retardation of follicle development in mosquito ovaries. Developing potent compounds counteracting JHaction (JH antagonists) would find a wider range of control applications. However, so far such JH antagonists have not been developed. Here, we report the discovery of potent JH antagonists in plants, which represents an innate resistance mechanism of plants against insect herbivores. These newly discovered plant JH antagonist compounds could be used as the starting material for developing novel insecticides.