Lack of TNF-α Gene Polymorphism (rs1799724) Association with Sustained Virological Response in Iranian Patients with Chronic HCV Infection

Larijani, Mona Sadat,Bahiraei, Narges,Nikbin, Mehri,Mohajel, Nasir,Rad, Leila Naghizadeh,Baghbani, Fahimeh,Mapar, Maryam,Sadat, Seyed Mehdi Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.8

Infection with the hepatitis C virus is a major public health concern which can lead to carcinoma and liver failure. It has been shown that single nucleotide polymorphisms can affect the level of gene activity of tumor necrosis factor (TNF) which has an important role, especially in viral infections which can lead to apaptosis of infected hepatocellular cells. We investigated the impact of three possible genotypes for rs1800629 or A/G single nucleotide polymorphism located downstream of $TNF{\alpha}$ gene promoter in groups of control (n=76) and chronic hepatitis C patients (n=89) focusing on the response to treatment among sensitive and resistant groups. Genomic DNA was extracted from $500{\mu}l$ prepheral whole blood and PCR and RFLP were used to amplify the region of interest and genotyping. With statistical analyzes a p-value <0.05 was considered meaningful. There was no significant difference in distribution of possible three genotypes among healthy individuals and patients (P=0.906, OR=1.194, CI=0.063-22.790). However, the frequency of G allele was higher in patients whereas A allele was more common among healthy individuals (p<0.0001). Further studies with more samples seem to be necessary.

Association of 8q24.21 rs10505477-rs6983267 Haplotype and Age at Diagnosis of Colorectal Cancer

Haerian, Monir Sadat,Haerian, Batoul Sadat,Rooki, Hassan,Molanaei, Saadat,Kosari, Farid,Obohhat, Maedeh,Hosseinpour, Parisa,Azimzadeh, Pedram,Mohebbi, Seyed Reza,Akbari, Zahra,Zali, Mohammad Reza Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.1

Background: Colorectal cancer (CRC) is the fourth most common cause of cancer death in the world. Genetic variants in 8q24.21 including rs10505477 and rs6983267 have been hypothesized to be involved in susceptibility to CRC. This study aims to investigate the possible association between these loci and their haplotypes with CRC risk in Iranian population. Materials and Methods: Subjects were recruited from two hospitals in Tehran. The rs10505477 and rs6983267 polymorphisms were genotyped by TaqMan real time PCR using subject genomic DNA, extracted either from formalin-fixed, paraffin-embedded tissue of patients or from blood of the controls by standard methods. Results: A total of 715 subjects (380 CRC patients and 335 matched controls) were genotyped in this study. Allele and genotype analysis of the rs10505477 and rs6983267 polymorphisms by gender, age at diagnosis, tumor location, tumor grade, and tumor node metastasis (TNM) showed no significant association with CRC risk. There was a significant relationship between GG haplotype and susceptibility to age at diagnosis for both <60 and ${\geq}60$ (p=0.0005 and p=0.000004, respectively) and between GT and CRC in the age at diagnosis ${\geq}60$ (Table 3: p=0.031). The GG haplotype was less frequent in CRC patients with the age at diagnosis <60, but was more common in subjects with the age at diagnosis ${\geq}60$. Conclusions: Results of this study suggests that the rs6983267 and rs10505477 polymorphisms alone may not be relevant to CRC risk, but their GG haplotype plays a notable role in age at diagnosis of CRC in the Iranian population.

Analysis of in planta Expressed Orphan Genes in the Rice Blast Fungus Magnaporthe oryzae

Sadat, Md. Abu,Jeon, Junhyun,Mir, Albely Afifa,Kim, Seongbeom,Choi, Jaeyoung,Lee, Yong-Hwan The Korean Society of Plant Pathology 2014 Plant Pathology Journal Vol.30 No.4

Genomes contain a large number of unique genes which have not been found in other species. Although the origin of such "orphan" genes remains unclear, they are thought to be involved in species-specific adaptive processes. Here, we analyzed seven orphan genes (MoSPC1 to MoSPC7) prioritized based on in planta expressed sequence tag data in the rice blast fungus, Magnaporthe oryzae. Expression analysis using qRT-PCR confirmed the expression of four genes (MoSPC1, MoSPC2, MoSPC3 and MoSPC7) during plant infection. However, individual deletion mutants of these four genes did not differ from the wild-type strain for all phenotypes examined, including pathogenicity. The length, GC contents, codon adaptation index and expression during mycelial growth of the four genes suggest that these genes formed during the evolutionary history of M. oryzae. Synteny analyses using closely related fungal species corroborated the notion that these genes evolved de novo in the M. oryzae genome. In this report, we discuss our inability to detect phenotypic changes in the four deletion mutants. Based on these results, the four orphan genes may be products of de novo gene birth processes, and their adaptive potential is in the course of being tested for retention or extinction through natural selection.

URANS simulations for a flooded ship in calm water and regular beam waves

Sadat-Hosseini, H.,Kim, D.H.,Carrica, P.M.,Rhee, S.H.,Stern, F. Pergamon Press 2016 Ocean engineering Vol.120 No.-

CFD simulations are conducted for zero-speed damaged passenger ship SSRC in calm water and waves with 6DOF motions including flooding procedure in calm water, roll decay in calm water and motions in regular beam waves for various wavelengths. The simulations model the 6DOF soft spring experimental mount, the one- and two-room flooding compartment configurations, including both intact and damaged conditions. For flooding and roll decay, simulations show ability to predict the trend of increases in roll period and damping due to flooding, as reported in ITTC. The damping magnitudes were often under-predicted with large errors while the roll period and compartment water height were well predicted. Two-room compartment simulation showed three times larger damping than one-room compartment cases whereas the roll period was similar for both conditions. For wave cases, all motions show primarily 1st order responses, except for parametric roll condition which shows large ½ harmonic responses for the intact ship. The 2nd order responses are small for both damaged and intact ship. The larger roll period and damping for the damaged ship shift the peak of responses to smaller wave frequency and reduce the amplitude of responses. The average error is often large for 1st order intact ship pitch and damaged ship surge and pitch. The errors are larger for most ½ and 2nd order responses. Large errors could be partially due to the complex mounting system in the experiment. Overall, current CFD results show better predictions than those reported for potential flow solvers even though the computational cost is larger.

A Novel Production Method for High-Fructose Glucose Syrup from Sucrose-Containing Biomass by a Newly Isolated Strain of Osmotolerant Meyerozyma guilliermondii

( Sadat Mohammad Rezq Khattab ),( Tsutomu Kodaki ) 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.4

One osmotolerant strain from among 44 yeast isolates was selected based on its growth abilities in media containing high concentrations of sucrose. This selected strain, named SKENNY, was identified as Meyerozyma guilliermondii by sequencing the internal transcribed spacer regions and partial D1/D2 large-subunit domains of the 26S ribosomal RNA. SK-ENNY was utilized to produce high-fructose glucose syrup (HFGS) from sucrose-containing biomass. Conversion rates to HFGS from 310-610 g/l of pure sucrose and from 75-310 g/l of sugar beet molasses were 73.5-94.1% and 76.2-91.1%, respectively. In the syrups produced, fructose yields were 89.4-100% and 96.5-100% and glucose yields were 57.6-82.5% and 55.3-79.5% of the theoretical values for pure sucrose and molasses sugars, respectively. This is the first report of employing M. guilliermondii for production of HFGS from sucrose-containing biomass.

Wheat Blast: A New Fungal Inhabitant to Bangladesh Threatening World Wheat Production

Sadat, Md. Abu,Choi, Jaehyuk The Korean Society of Plant Pathology 2017 Plant Pathology Journal Vol.33 No.2

World wheat production is now under threat due to the wheat blast outbreak in Bangladesh in early March 2016. This is a new disease in this area, indicating the higher possibility of this pathogen spreading throughout the Asia, the world's largest wheat producing area. Occurrence of this disease caused ~3.5% reduction of the total wheat fields in Bangladesh. Its economic effect on the Bangladesh wheat market was little because wheat contributes to 3% of total cereal consumption, among which ~70% have been imported from other countries. However, as a long-term perspective, much greater losses will occur once this disease spreads to other major wheat producing areas of Bangladesh, India, and Pakistan due to the existing favorable condition for the blast pathogen. The wheat blast pathogen belongs to the Magnaporthe oryzae species complex causing blast disease on multiple hosts in the Poaceae family. Phylogenetic analysis revealed that the Bangladesh outbreak strains and the Brazil outbreak strains were the same phylogenetic lineage, suggesting that they might be migrated from Brazil to Bangladesh during the seed import. To protect wheat production of Bangladesh and its neighbors, several measures including rigorous testing of seed health, use of chemicals, crop rotation, reinforcement of quarantine procedures, and increased field monitoring should be implemented. Development of blast resistant wheat varieties should be a long-term solution and combination of different methods with partial resistant lines may suppress this disease for some time.

Parametric Study of FRP Strengthening on Stress Concentration Factors in an Off shore Tubular T-Joint Subjected to In-Plane and Out-of-Plane Bending Moments

Alireza Sadat Hosseini,Mohammad Reza Bahaari,Mohammad Lesani 한국강구조학회 2019 International Journal of Steel Structures Vol.19 No.6

This paper describes numerical investigations conducted on a tubular T-joint strengthened by FRP composites under in-plane bending (IPB) and out-of-plane bending (OPB) moments. The FRP materials were applied as a protective layer on the T-joint, and the analyzed models were eventually validated against the results obtained from an experimental study. The eff ectiveness of using FRP materials on enhancing the fatigue life of a tubular T-joint was investigated through computing the relative SCFs of 150 fi nite element models using ABAQUS software package. Analyses have been performed to investigate diff erent FRP parameters such as fi ber orientation, FRP thickness, wrapping length and FRP material properties of the wrapped joint. Eff ectiveness of these FRP parameters on SCF reduction along with the most effi cient lay-ups against both IPB and OPB moments were reported. According to the results of this study, further reduction in SCFs was observed when the joint was confi ned with stiff er FRPs. SCF reduction of around 15% was observed for only a 1 mm thick CFRP layup. Moreover, using a thicker FRP layup tends to increase its eff ectiveness signifi cantly. However, it was observed that increasing the FRP wrapping length has negligible eff ect on SCF values, and has to be chosen so as to cover all the chord surface area infl uenced by the brace loading in order to balance the state of stresses and deformations.

Recovery of Acute Ophthalmoplegia after Hyaluronic Acid Filler Injections to the Temples: A Case Report and Review of the Literature

Fatemeh-sadat Tabatabaei 대한성형외과학회 2023 Archives of Plastic Surgery Vol.50 No.2

Transition nuclear protein 1 as a novel biomarker in patients with fertilization failure

Jamileh Sadat Mirsanei,Hadis Gholipour,Zahra Zandieh,Masoumeh Golestan Jahromi,Mojgan Javedani Masroor,Mehdi Mehdizadeh,Fatemehsadat Amjadi The Korean Society for Reproductive Medicine 2023 Clinical and Experimental Reproductive Medicine Vol.50 No.3

Objective: Although intracytoplasmic sperm injection (ICSI) is a way to deal with in vitro fertilization failure, 3% of couples still experience repeated fertilization failure after attempted ICSI, despite having sperm within normal parameters. These patients are a challenging group whose sperm cannot fertilize the egg during ICSI. Unfortunately, no test can predict the risk of fertilization failure. Phospholipase C zeta (PLCζ) and transition nuclear proteins (TNPs) are essential factors for chromatin packaging during sperm maturation. This study aimed to assess PLCζ1 and TNP1 expression in the sperm of patients with fertilization failure and the correlations among the DNA fragmentation index, PLCζ1 and TNP1 gene and protein expression, and the risk of fertilization failure. Methods: In this study, 12 infertile couples with low fertilization rates (<25%) and complete failure of fertilization in their prior ICSI cycles despite normal sperm parameters were chosen as the case group. Fifteen individuals who underwent ICSI for the first time served as the control group. After sperm analysis and DNA fragmentation assays, quantitative reverse-transcription polymerase chain reaction (qRT-PCR) and Western blot analyses were performed to compare the gene and protein expression of PLCζ and TNP1 in both groups. Results: DNA fragmentation was significantly higher in the fertilization failure group. The qRT-PCR and Western blot results demonstrated significantly lower PLCζ and TNP1 gene and protein expression in these patients than in controls. Conclusion: The present study showed that fertilization failure in normozoospermic men was probably due to deficient DNA packaging and expression of TNP1.

Calculating the Contact Stress Resulting from Lateral Movement of the Wheel on Rail by Applying Hertz Theory

Roya Sadat Ashofteh 한국철도학회 2013 International Journal of Railway Vol.6 No.4

This article has tried to review the maximum contact stresses in the contact area of the wheel and rail as a result of lateral movement of the wheel on rail by taking advantage from Hertz theory. Since wheel movement on rail is accompanied by lateral movement due to wheel profile conisity, so the contact point of wheel and rail is not constant and the contact stresses are therefore changeable in every single moment. Since the shape of rail profile and rail inclination, wheel diameter and the mechanical properties of the wheel and rail are effective on the stresses of contact area, these parameters have been studied by applying Hertz theory. This article aims to calculate the contact stresses in different parts on the wheel surface by using Hertz theory.