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TNR Gene Mutation in Familial Parkinson’s Disease: Possible Implications for Essential Tremor
Michel Sáenz-Farret,Renato Puppi Munhoz,Alfonso Fasano,Carlos Zúñiga-Ramírez 대한파킨슨병및이상운동질환학회 2021 Journal Of Movement Disorders Vol.14 No.2
Farlow et al.1 reported in 2016 new rare tyrosine kinase nonreceptor2 and tenascin R (TNK2 and TNR, respectively) variantsassociated with the development of Parkinson’s disease(PD). No detailed clinical information about such patients orother reports is available thus far. We report a subject with PDand two of his sons with essential tremor (ET) harboring a TNRgene mutation.
Julián David Salazar-Gutiérrez,Andrés Castelblanco,María Ximena Rodríguez-Bocanegrab,Wilson Teran,Adriana Sáenz-Aponte 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol.20 No.4
Entomopathogenic nematodes are key players for insect pest control and constitute an environmentally friendly alternative for crop protection. The insecticidal activity of the family Heterorhabditidae relies on a tight symbiotic relationship with enterobacteria of the genus Photorhabdus, where the bacterial contribution towards the death of the host has been highlighted. In the present work, we report the identification and pathogenic characterization of Photorhabdus luminescens strain SL0708, which is the natural symbiont of Heterorhabditis indica SL0708. We evaluated the pathogenicity of whole bacterial cells and acellular extracts against both Spodoptera frugiperda larvae and Galleria mellonella. Phylogenetic analyses using a polygenic sequencing approach assigned the bacterial strain to Photorhabdus luminescens subsp. akhurstii and bioassays showed it is highly pathogenic for both insects. After 48 h of treatment with 1× 103 −1 × 104 CFU/larva, 100% mortality was attained. Furthermore, when intra- or extracellular bacterial extracts were injected into G. mellonella, a cumulative percent mortality of 63% and 100% was respectively obtained after 72 h. In contrast, a 10% and 93% mortality was achieved for S. frugiperda with intra and extracellular extracts, respectively highlighting the role of extracellular factors in pathogenicity. We detected extracellular activities potentially accounting for the high pathogenicity observed and these included; proteases, esterases, ureases, hemolysins and siderophores. Interestingly, S. frugiperda was more susceptible to P. luminescens SL0708 cells than G. mellonella, which contrasted to its higher resistance to H. indica SL0708 nematodes, which suggests that EPN biological control potential should also be evaluated based on bacterial symbiont pathogenicity
Enterobacter cloacae, an Emerging Plant-Pathogenic Bacterium Affecting Chili Pepper Seedlings
Tanahiri García-González,Hilda Karina Sáenz-Hidalgo,Hilda Victoria Silva-Rojas,Carlos Morales-Nieto,Taca Vancheva,Ralf Koebnik,Graciela Dolores Ávila-Quezada 한국식물병리학회 2018 Plant Pathology Journal Vol.34 No.1
A previously unreported bacterial disease on chili pepper (Capsicum annuum L.) seedlings affecting as many as 4% of seedlings was observed in greenhouses in Chihuahua, Mexico (Delicias and Meoqui counties). Initial lesions appeared as irregular small spots on leaves and brown necrosis at margins tips were observed. Later, the spots became necrotic with a chlorotic halo. Advanced disease was associated with defoliation. A Gram negative, rod-shaped bacterium was isolated from diseased chili pepper seedlings. Three inoculation methods revealed that isolated strains produce foliage symptoms, similar to those observed in naturally infected seedlings. Pathogenic strains that caused symptoms in inoculated seedlings were re-isolated and identified to fulfill koch´s postulate. Polyphasic approaches for identification including biochemical assays (API 20E and 50CH), carbon source utilization profiling (Biolog) and 16S rDNA, hsp60 and rpoB sequence analysis were done. Enterobacter cloacae was identified as the causal agent of this outbreak on chili pepper seedlings.
Ortiz-Gómez Tamin,Toledano Paula,Gomez Andrea C.,López María,Alonso Carla Andrea,Ruiz Joaquim,Lagos José,Sáenz Yolanda 대한감염학회 2023 Infection and Chemotherapy Vol.55 No.2
Stenotrophomonas maltophilia is an opportunistic pathogen, often associated with nosocomial infections. Ten S. maltophilia were isolated from clinical samples during the period January 2021 and June 2022. Eight (80%) patients had cancer as a background disease and 2 patients had coronavirus disease 2019. A fatal outcome was recorded in 4 cases (40% of patients). All the isolates were susceptible to minocycline and levofloxacin. Trimethoprim/sulfamethoxazole and ceftazidime resistance rates were 20% and 40% respectively. Eight different patterns were observed by Pulsed-Field Gel Electrophoresis, only two isolates being clonally identical. The isolation of S. maltophilia in clinical settings requires the implementation of infection prevention measures.
Elisabet Benedicto,Eva María Rubio,Laurent Aubouy,María Ana Sáenz-Nuño 한국정밀공학회 2023 International Journal of Precision Engineering and Vol.10 No.3
This paper presents the study of the finishing, repair, and maintenance turning operations of gamma titanium aluminide (γ-TiAl) parts from the aeronautic industry, with the aim to evaluate different sustainable lubrication/cooling environments, including a newly developed synthetic ester water-based metalworking fluid (EcoMWF) to replace mineral-based MWF (MWF). The systems considered in this work are dry, cold-compressed air, minimum quantity lubrication (MQL), cryogenic, and flood on turning of a new and relatively low explored titanium alloy, γ-TiAl. Therefore, the influence of machining parameters and insert type on tool wear, surface roughness, roundness, and cutting temperature have been investigated for each environment. Results detailed in this study showed a significant influence of the lubrication/cooling systems on the machinability of γ-TiAl. The study also revealed that the sustainability of turning γ-TiAl could be improved under the cryogenic system and the new EcoMWF, keeping the same machining performance as common mineral-based MWF.
Germán Nic‑Matos,María Narváez,Santy Peraza‑Echeverría,Luis Sáenz,Carlos Oropeza 한국유전학회 2017 Genes & Genomics Vol.39 No.9
Nonexpressor of pathogenesis-related gene 1 (NPR1) codes for a transcription cofactor involved in the activation of systemic acquired resistance, a salicylic acid (SA)-dependent defense response. This work reports the cloning and characterization of two new genes, CnNPR1 and CnNPR3 from coconut, homologous to AtNPR1 of Arabidopsis thaliana. The cDNA deduced amino acid sequence contains the protein–protein interaction domains the BTB/POZ and ANKYRIN repeat domains, and a nuclear localization site (NLS). Phylogenetic analysis grouped CnNPR1 in a clade with AtNPR1 and CnNPR3 in a clade with AtNPR3, both reported genes of A. thaliana. Exogenous application of SA to coconut plantlets induced changes in the expression of CnNPR1 and CnNPR3 in leaf, stem and root tissues, providing evidence of their possible role in the signaling cascade leading to SAR in coconut palm. This is the first report on the cloning of putative key genes in the SAR-type defense mechanism in coconut palm.
Transcriptome analysis reveals key defense-related genes upon SA induction in Cocos nucifera L.
C. Silverio-Gómez,J. Vega-Arreguín,G. Nic-Matos,M. Narváez-Cab,L. Sáenz-Carbonell,C. Oropeza 한국유전학회 2022 Genes & Genomics Vol.44 No.2
Background: Salicylic acid (SA) is an important regulator of genes involved in plant defense and pathogen-triggered systemic acquired resistance (SAR). Coconut is an important crop affected by several pathogens. Reported evidence suggests SA involvement in defense responses, including SAR in coconut. Objective: To identified differentially expressed genes in leaf and root tissues of coconut plantlets, as a result of SA, that might be involved in coconut defense responses. Methods: Comparative transcriptomic analysis by RNA-Seq of leaf and root tissues from in vitro coconut plantlets unexposed and exposed to SA 2.5 mM for 48 h. And in silico validation of gene expression by qRT-PCR. Results: We identified 4615 and 3940 differentially expressed unigenes (DEUs) in leaf and root tissues respectively. Our GO analysis showed functional categories related to the induction of defense responses, such as "systemic acquired resistance" and highly enriched hormone categories, such as abscisic acid. The most abundant KEGG pathway in our results was "Biosynthesis of antibiotics". Our findings support that exogenous application of SA to plantlets induced the activation of PRs, RGAs, ICS2, NLTP2, PER4, TRXM and some WRKYs mediated by NPR1-dependent pathways. Also, we found DEUs, such as BZR1, HSL1, and WHY2 that support that SA could regulate defense-related genes through NPR1-independent pathways. Conclusion: The present study of massive data analysis carried out on coconut plantlets exposed to SA, generates valuable information that increases our understanding of defense molecular mechanisms in coconut and open new venues for research for the improvement of management of coconut diseases.
Edwin David Morales-Álvarez,Claudia Marcela Rivera-Hoyos,Angélica María Baena-Moncada,Patricia Landázuri,Raúl A. Poutou-Piñales,Homero Sáenz-Suárez,Luis A. Barrera,Olga Y. Echeverri-Peña 한국미생물학회 2013 The journal of microbiology Vol.51 No.2
The sulfatase family involves a group of enzymes with a large degree of similarity. Until now, sixteen human sulfatases have been identified, most of them found in lysosomes. Human deficiency of sulfatases generates various genetic disorders characterized by abnormal accumulation of sulfated intermediate compounds. Mucopolysaccharidosis type II is characterized by the deficiency of iduronate 2-sulfate sulfatase (IDS), causing the lysosomal accumulation of heparan and dermatan sulfates. Currently, there are several cases of genetic diseases treated with enzyme replacement therapy, which have generated a great interest in the development of systems for recombinant protein expression. In this work we expressed the human recombinant IDS-Like enzyme (hrIDS-Like) in Escherichia coli DH5α. The enzyme concentration revealed by ELISA varied from 78.13 to 94.35 ng/ml and the specific activity varied from 34.20 to 25.97 nmol/h/mg. Western blotting done after affinity chromatography purification showed a single band of approximately 40 kDa, which was recognized by an IgY polyclonal antibody that was developed against the specific peptide of the native protein. Our 100 ml-shake-flask assays allowed us to improve the enzyme activity seven fold,compared to the E. coli JM109/pUC13-hrIDS-Like system. Additionally, the results obtained in the present study were equal to those obtained with the Pichia pastoris GS1115/pPIC-9-hrIDS-Like system (3 L bioreactor scale). The system used in this work (E. coli DH5α/pGEX-3X-hrIDS-Like)emerges as a strategy for improving protein expression and purification, aimed at recombinant protein chemical characterization,future laboratory assays for enzyme replacement therapy, and as new evidence of active putative sulfatase production in E. coli.