http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Detection and Isolation of Dendritic Cells Using Lewis X-functionalized Magnetic Nanoparticles
Sara H. Rouhanifard,Ran Xie,Guoxin Zhang,Xiaoming Sun,Xing Chen,Peng Wu 한국당과학회 2012 한국당과학회 학술대회 Vol.2012 No.1
Dendritic cells (DCs) are professional antigen-presenting cells that serve as messengers between innate and adaptive immunity. Due to their unique roles, DCs are under active investigations as the target for antigen delivery in vaccination against human immunodeficiency virus (HIV), cancer and autoimmune diseases. However, there’s yet no perfect strategy for a cost-effective, high-selective and time-saving detection and isolation of DCs from a complex cell population. Adhesion molecules such as DC-specific intracellular adhesion molecule-3 grabbing non-integrin (DC- SIGN) on the surface of DCs play a critical role in establishing contact between the activated DCs and the naïve T cells through interactions with intercellular adhesion molecule-3 on T cells. DC-SIGN is a member of the C-type lectin receptors and can recognize antigens bearing mannose-rich or fucosylated glycans, as well as Lewis X (LeX). Here, we present the fabrication of magnetic nanoparticles coated with multivalent LeX glycans using the Cu (I)-catalyzed azide-alkyne cycloaddition. The resulting nanoparticles are selective and biocompatible, serving as a highly efficient tool for DC capture detection and enrichment.