Effect of Chromatin Changes in the Germinal Vesicle on the Development of Porcine Embryos In Vitro

Min‐Gu Lee,Rong‐xun Han,Jin‐Yu zhang,Yun‐Fei Diao,Reza K. Oqani,Dong‐Il Jin 한국동물생명공학회(구 한국동물번식학회) 2011 발생공학 국제심포지엄 및 학술대회 Vol.2011 No.1

In all the studies of mammalian species, chromatin in the germinal vesicle (GV) is initially decondensed with the nucleolus not surrounded by heterochromatin (the NSN configurations). During oocyte growth, the GV chromatin condenses into perinucleolar rings (the SN configurations) or other corresponding configurations with or without the perinucleolar rings, depending on species. During oocyte maturation, the GV chromatin is synchronized in a less condensed state before germinal vesicle breakdown (GVBD) in species that has been minutely studied. As not all the species show the SN configuration and gene transcription always stops at the late stage of oocyte growth, it is suggested that a thorough condensation of GV chromatin is essential for transcriptional repression. Because the GV chromatin status is highly correlated with oocyte competence, oocytes must end the NSN configuration before they gain the full meiotic competence and they must take on the SN/corresponding configurations and stop gene transcription before they acquire the competence for early embryonic development. In this study, we firstly investigated whether the layer of cumulus cells and size of oocytes could determine chromatin configurations in porcine oocytes. Using Hoechst3342 staining, the GV nucleolus and chromatin of porcine oocytes was classified into SN and NSN configurations. Next, we examined the changes in GV chromatin configurations during growth and maturation of porcine oocytes. In addition, the maturation and parthenogenetic development abilities in vitro were significant different between the SN and NSN configurations oocytes. These results indicated that chromatin changes in GV oocytes affect the development potential of parthenogenetic embryos.

두개안면 섬유성 이형성증의 임상적 고찰

백롱민,오갑성,윤성호,백세민,황민호 大韓成形外科學會 1994 Archives of Plastic Surgery Vol.21 No.3

Fibrous dysplasia is a benign pathologic condition of bone and causes facial deformities as well as functional problems. We have managed 32 craniofacial fibrous dysplasia form March 1989 to December 1993. The most common age distribution of surgery was second decade(34%) and third decade(53%). In our patients, the desease process usually begins in childhood and progresses throughout puberty and adolescence. Treatment was aimed at preventing and correcting functional problems and achieving normal facial aesthetics. The reconstruction with other combined surgery, i.e., mandibular and maxillary osteotomies, reduction malar plasty, malar augmentation and orbit, nasal reconstruction with bone graft. Postoperative results were satisfactory in most cases. In some patients, repeated contouring(16%) surgery might produce less morbidity than a single massive resection. In evaluating the extent of the lesion, computerized tomography prooved to be a good diagnostic tool. Aethetically and functionally satisfactory surgical results were achieved by bone shaving in combination with reconstructive surgery.

HYDROXYAPATITE를 이용한 전두부 성형술

백롱민,박재현,김진환,백세민,오갑성,정연철 大韓成形外科學會 1993 Archives of Plastic Surgery Vol.20 No.5

The shape and contour of the upper third of the face is provided basically by the frontal bone. A relatively thin soft tissue coverage, which consists of the skin and frontalis muscle, readily shows subtle changes on the underlying frontal bone. The forehead represents the fortune of one's youth from the viewpoint of physignomy, and a broad forehead with minimal supraorbital bossing and round curvature is preferrd in the Orient. The forehead with a deficient, flat contour or asymmetry could be indicated for augmentation. The exact site and amount of augmentation are dicided preoperatively using clinical assessment, medical photographs, and cephalometric analysis. Hydroxyapatite(Pyrost), because of its nonresorbability, seems to have advantages over other alloplastic materials for forehead augmentation. From March 1989 to February 1992 forehead augmentation has been performed in 60 patients. Through a coronal incision, the dissection was carried over the periosteum to the supraorbital ridge and the lateral orbital rim. The subperiosteal pocket was made through a small incision, and was packed with Pyrost. When indicated, facial rhytidectomy, or reduction malar plasty was performed simultaneously. The result had been satisfactory and long lasting. There had been no implant shifting, infection, or bony erosion when examed from 1 year to 4 years after surgery.

유리피판술을 이용한 진행성 반안면 위축증(Romberg's Desease)의 치험

백롱민,정두성,백세민 大韓成形外科學會 1999 Archives of Plastic Surgery Vol.26 No.5

Romberg's disease is an uncommon condition manifested by progressive hemifacial atrophy of the skin, soft tissue and bone. Facial asymmetry with soft tissue deficiency in Romberg's disease causes a significant disability affecting the social life and can bring about many psychological problems. The aim of surgical treatment is cosmetic amelioration of the defect. Several conventional reconstructive procedures have been used for correcting facial asymmetry. They include fat injections, dermal fat grafts, silicone injections, cartilage and bone grafts, pedicled flaps and free flaps. We report our experience with 6 patients involving 6 free flaps with a minimun of 1 year follow-up who were treated from October 1989 to March 1998. All patients were classified as having moderate to severe atrophy. The average age of disease onset was 4.5 years. The average duration of atrophy was 5.2 years. No patient was operated on with a quiescent interval of less than 1 year. The average age at operation was 14.1 years, ranging from 10 to 24 years. Follow-up ranged from 1 to 9 years. Reconstruction was performed using 2 groin dermofat free flaps and 4 latissimus dorsi muscular free flaps. To achieve the finest symmetrical and aesthetic results, several ancillary procedures were performed in 4 patients. These procedures included Le-Fore 1 ½ leveling osteotomy, sagittal split ramus osteotomy, reduction malarplasty and angleplasty, rib and calvarial bone graft, correction of alopecia and additional dermofat graft. All patients were satisfied with the results. We believe that free flap is one of the best choices for contoured restoration of facial asymmetry in Romberg's disease.

대구증의 수술적 요법

백롱민,박종철,허찬영 大韓成形外科學會 2003 Archives of Plastic Surgery Vol.30 No.5

非星相刑 集合에서의 非擴張的인 寫像의 固定點들에 관한 硏究

이용녹,趙玟亨 金烏工科大學校 1990 論文集 Vol.11 No.-

본 연구에서 우리는 starshaped set이 포함되는 족(family)에서의 복소선형공간 안에 있는 새로운 부분집합류를 소개하고 몇 가지 일반화된 고정점정리를 얻어내었다.

관골 축소술에 대한 임상적 고찰

백세민,함정욱,백롱민,오갑선 大韓成形外科學會 1997 Archives of Plastic Surgery Vol.24 No.6

Orientals with prominent cheek bones are aesthetically unpleased. So we have performed reduction malarplasty which consists of medial and lateral malar osteotomies, mobilization of malar complex to more attractive point, and wire fixation through coronal incision on 288 patients from March, 1988 to February, 1997. Results have shown symmetry, natural contour, good patient acceptance, and no significant complication. The concept of 'mobilization of malar eminence to more attractive point' is important because the goal of surgery is not to achieve merely 'flat' malar complex. At the same time various procedures have been also performed to improve facial aesthetics. The simultaneous subperiosteal face life was advantageous especially to aged patients and to those with severe anterior projection of malar eminences. Reduction malarplasty combined with reduction angleplasty and face life is one of the useful way to restore the youthful appearance.

하악각축소술시 절제된 골의 간치이식을 이용한 턱끝증대술

이태일,백룡민,오갑성,백세민 大韓成形外科學會 1997 Archives of Plastic Surgery Vol.24 No.5

The square face or the box face appearance is quite common in orientals as well as occidentals and consists of flaring at the mandible angle areas with exaggerated posterior inferior projection of the mandibular angle in combination with a small and weak chin, resulting in widened bigonial distance and shortened lower third of face. Such patients complain of unbalanced and masculine appearance and may regard themselves as unaesthetic. To correct these deformities simultaneously, the mandible angles were resected (angled osteotamy) and the bone fragment from these ostectomized mandibular angles were used to augment the chin as an autogenous interpositional bone graft. At the Institute of Plastic and Reconstruction Craniofacial Surgery(IPRCS), Ice University Collage of Medicine, twenty eight cases were successfully operated in this manner during a six-year period from June 1989 to May 1995. Patient's satisfaction was high and improved esthetics were noted in all patients with minimal complications. The advantages are: l. Simultaneous correction of prominent mandible angle and augmentation of the deficient chin area. 2. Provides vertical elongation of the chin and lower face, which gives visual compensation for agittal weakness of the lower face. 3. Improves upper and lower facial balance. 4. Another donor site for bone is not necessary, thus avoiding unnecessary donor site morbidity. 5. Homogenous autologous bone is used thus reducing the chance of resorption. 6. Minimal chance of infection.

심한 안검하수에 있어서 측두근막과 Tutoplast Dura를 이용한 전두근 현수법

허찬영,백롱민,백세민 大韓成形外科學會 1999 Archives of Plastic Surgery Vol.26 No.4

Patients with a poor or total absence of levator function require correction of their ptosis by a sling procedure. The aim of the procedure is to utilize the action of the frontalis muscle to mechanically raise a droopy eyelid Frontalis suspension procedure using a temporalis fascia or Tutoplast Dura was performed on 36 ptotic eyelids of 23 patients(age range 2-37 years) with a poor or absent levator function. The author used a surgical technique based on a modified Fox`s method. Satisfactory results were achieved in 42 lids according to the criteria of Jordan after an average follow-up of 23 months. No serious complication was seen immediately postoperative and during the follow-up period. Temporalis fascia and Tutoplast Dura appear to be effective and safe alternative sling materials for frontalis suspension surgery.

턱끝절골술 : 189명에 대한 고찰

오갑성,백룡민,이태일,임재호,백세민 대한미용성형외과학회 1997 Archives of Aesthetic Plastic Surgery Vol.3 No.1

The chin plays a decisive role in the contour of the lower face. And harmonious jaw will correspond to good facial profile and positive image. Osseous genioplasty is a widely used method to correct the 3-dimensional(sagittal, vertical, transverse) deformity of the chin with minimal complications. During an 8 year period from 1988, the authors at the In-Je University Seoul Paik Hospital experienced 198 patients of osseous genioplasty. Results of clinical analysis are as follows: 1)Mail to female ratio was 1 : 3 with female being predoniment. The average age was 24.9 years. 2) Horizontal advancement genioplasty in 169 cases was the most common precedure with 7.0mm of average advancement. There was 4 cases of horizontal retrusion with average retrusion of 4.3mm. 52 cases had vertical augmentation with average of 7.5mm increase. 9 cases had vertical reduction with average redution of 4.9mm. 3) Although malocclusion is not necessarilly contraindication to genioplasty alone, genioplasty following surgical with or without orthodontical correction of malocclusion is the way to get better result with patient satisfaction. 4) 77% of the patients had adjunctive procedures simultaneously to acheive a better contour and profile 5) Complications such as hematoma, infection, fracture, nerve damage were minimal.