<i>Hypnea musciformis</i>(Cystocloniaceae) from the Yucatan Peninsula: morphological variability in relation to life-cycle phase

Vá,zquez-Delfí,n, Erika,Boo, Ga Hun,Rodrí,guez, Dení,Boo, Sung Min,Robledo, Daniel Informa UK (Taylor Francis) 2016 Phycologia Vol.55 No.2

Progression of Jackhammer Esophagus to Type III Achalasia and Improvement After Extended Myotomy

( Pablo Vázquez García ),( Constanza Ciriza De Los Ríos ),( Fernando Canga Rodríguez-valcárcel ),( Diego Hernández García-gallardo ) 대한소화기 기능성질환·운동학회 2020 Journal of Neurogastroenterology and Motility (JNM Vol.26 No.1

Chitosan-Hydroxyapatite Scaffold for Tissue Engineering in Experimental Lumbar Laminectomy and Posterolateral Spinal Fusion in Wistar Rats

Martin Rodríguez-Vázquez,Rodrigo Ramos-Zúñiga 대한척추외과학회 2020 Asian Spine Journal Vol.14 No.2

Study Design: Experimental study with an animal model. Purpose: To evaluate the role of a chitosan and hydroxyapatite composite for spinal fusion in a lumbar experimental model based on regenerative tissue engineering principles. Overview of Literature: Chitosan and hydroxyapatite represent an alternative biodegradable implant material for tissue engineering and regeneration. The combination of chitosan and hydroxyapatite in a 20:80 ratio could potentiate their individual properties as an implantable composite for experimental laminectomy. Methods: Phase I: design and synthesis of a porous composite scaffold composed of chitosan-hydroxyapatite using a freeze drying technique. Phase II: experimental microsurgical lumbar laminectomy at L5. A total of 35 Wistar rats were categorized into three experimental groups: control (laminectomy alone), experimental (laminectomy with implant), and reference (intact spine) (n=5 per group). Postoperative structural and functional evaluations were performed using computed tomography scans. In addition, radiologic, clinical, histological, and immunohistochemical microstructures were evaluated. Results: At the laminectomy site, the composite implant induced bone regeneration, which was observed in the axial reconstruction of the rat lumbar spine in all cases. Biomechanical changes in the lumbar spine were observed by radiology in both groups after the surgery. The posterolateral space was covered by a bone structure in the treated spine, a condition not seen in the control group. The range of motion was 7.662°±0.81° in the scaffold group versus 20.72°±3.47° in the control group. Histological findings revealed qualitatively more bone tissue formation in the implant group. Conclusions: A composite of chitosan-hydroxyapatite at a 20:80 ratio induced bone formation after experimental laminectomy in rats and led to spinal fusion, which was assessed by radiology and biomechanical tests. No functional complications in posture or walking were observed at 90 days post-surgery, despite biomechanical changes in the spine.

Antibacterial Properties of Honey Produced by Melipona beecheii and Apis mellifera against Foodborn Microorganisms

David Chan-Rodríguez,Jesús Ramón-Sierra,Jorge Lope-Ayora,Enrique Sauri-Duch,Luis Cuevas-Glory,Elizabeth Ortiz-Vázquez 한국식품과학회 2012 Food Science and Biotechnology Vol.21 No.3

The aim of this study was to determine the antibacterial activity in vitro of Melipona and Apis honey against various foodborne microorganisms. The results of qualitative test showed that Melipona honey inhibited both Staphylococcus aureus ATCC25923 and H08M06 strains and both Escherichia coli ATCC35922 and H12K06 strains. Apis honey inhibited both E. coli ATCC35922 and H12K06strains and S. aureus ATCC25923 strain. Minimum inhibitory concentrations of Melipona honey (5-16%, w/v) were lower than those of Apis honey (15-31%, w/v). This study demonstrated that the antibacterial potency of Melipona honey could be a new alternative for controlling foodborne illness.

Qualitative changes in fetal trabecular meshwork fibers at the human iridocorneal angle

Fumio Hosaka,Jose Francisco Rodríguez-Vázquez,Hiroshi Abe,Gen Murakami,Mineko Fujimiya,Hiroshi Ohguro 대한해부학회 2013 Anatomy & Cell Biology Vol.46 No.1

We examined a series of changes that occur in the trabecular meshwork fibers of human eyes during fetal development at 12-30 weeks of gestation. At 12 and 15 weeks, the uveal meshwork was stained black with silver impregnation (indicating the predominance of collagen types III and IV) in the endomysium of the ciliary muscle. At 20 weeks, in combination with Schlemm’s canal, a dense fibrous tissue mass corresponding to the trabecular meshwork anlage appeared and was colored black. The anlage was continuous with the corneal endothelium rather than with the ciliary muscle. Until 25 weeks, the trabecular meshwork was identifiable as fragmented fiber bundles that stained red-black, suggesting a mixture of collagen types I, III, and IV. At 30 weeks, half of the ciliary muscle fibers were inserted into the scleral spur and not into the meshwork. Therefore, any contribution of ciliary muscle contraction to the differentiation of the trabecular meshwork would appear to be limited. We hypothesize that an uneven distribution of mechanical stresses in the area of the cornea-sclera junction causes a tear thereby creating Schlemm’s canal and is accompanied by a change in the collagen fiber types comprising the meshwork.

Fractional Sliding Mode Control of Underwater ROVs subject to Non-differentiable Disturbances

Aldo-Jonathan Muñoz-Vázquez,Heriberto Ramírez-Rodríguez,Vicente Parra-Vega,Anand Sánchez-Orta 제어·로봇·시스템학회 2017 International Journal of Control, Automation, and Vol.15 No.3

Some hydrodynamic phenomena of an underwater Remotely Operated Vehicle (ROV), such as turbulence,cavitation, and multi-phase fluidic regimes, are associated to continuous but nowhere differentiable functions. These disturbances stand as complex forces potentially influencing the ROVs during typical navigation tasks. In thispaper, the tracking control of a ROV subject to nonsmooth Hölder disturbances is proposed based on a fractionalorderrobust controller that ensures exponential tracking. Notably, the controller gives rise to a closed-loop systemwith the following characteristics: a) continuous control signal that alleviates chattering effects; b) the fractionalsliding motion is substantiated on a proposed resetting memory principle; c) the control is robust to model uncertainties;and d) exact rejection of Hölder disturbances in finite-time. A representative simulation study reveals thefeasibility of the proposed scheme.

Development of the Rectus Abdominis and Its Sheath in the Human Fetus

양재도,조백환,황홍필,김지현,Jose Francisco Rodríguez-Vázquez,Shin-ichi Abe,Gen Murakami 연세대학교의과대학 2012 Yonsei medical journal Vol.53 No.5

Purpose: Although the rectus abdominis and its sheath are well known structures, their development in the human fetus is poorly understood. Materials and Methods:We examined rectus abdominis and sheath development in semiserial horizontal sections of 18 fetuses at 5-9 weeks of gestation. Results: Rectus muscle differentiation was found to commence above the umbilicus at 6 weeks and extend inferiorly. Until closure of the anterior chest wall via fusion of the bilateral sternal anlagen (at 7 weeks), the anterior rectal sheath originated from the external oblique and developed towards the medial margin of the rectus abdominis at all levels, including the supracostal part. After formation of the anterior sheath, fascial laminae from the internal oblique and transversus abdominis contributed to formation of the posterior rectus sheath. However, the posterior sheath was absent along the supracostal part of the rectus abdominis, as the transversus muscle fibers reached the sternum or the midline area. Therefore, it appeared that resolution of the physiological umbilical hernia (8-9 weeks) as well as chest wall closure was not required for development of the rectus abdominis and its sheath. Conversely, in the inferior part of the two largest fetal specimens, after resolution of the hernia, the posterior sheath underwent secondary disappearance, possibly due to changes in mechanical stress. Conclusion: Upward extension of the rectus abdominis suddenly stopped at the margin of the inferiorly developing pectoralis major without facing the external intercostalis. The rectus thoracis, if present, might correspond to the pectoralis.

Nervus terminalis and nerves to the vomeronasal organ: a study using human fetal specimens

Zhe Wu Jin,조광호,Shunichi Shibata,Masahito Yamamoto,Gen Murakami,Jose Francisco Rodríguez-Vázquez 대한해부학회 2019 Anatomy & Cell Biology Vol.52 No.3

The human nervus terminalis (terminal nerve) and the nerves to the vomeronasal organ (VNON) are both associated with the olfactory nerves and are of major interest to embryologists. However, there is still limited knowledge on their topographical anatomy in the nasal septum and on the number and distribution of ganglion cells along and near the cribriform plate of the ethmoid bone. We observed serial or semiserial sections of 30 fetuses at 7–18 weeks (crown rump length [CRL], 25–160 mm). Calretinin and S100 protein staining demonstrated not only the terminal nerve along the anterior edge of the perpendicular lamina of the ethmoid, but also the VNON along the posterior edge of the lamina. The terminal nerve was composed of 1–2 nerve bundles that passed through the anterior end of the cribriform plate, whereas the VNON consisted of 2–3 bundles behind the olfactory nerves. The terminal nerve ran along and crossed the posterior side of the nasal branch of the anterior ethmoidal nerve. Multiple clusters of small ganglion cells were found on the lateral surfaces of the ethmoid’s crista galli, which are likely the origin of both the terminal nerve and VNON. The ganglions along the crista galli were ball-like and 15–20 µm in diameter and, ranged from 40-153 in unilateral number according to our counting at 21-μm-interval except for one specimen (480 neurons; CRL, 137 mm). An effect of nerve degeneration with increasing age seemed to be masked by a remarkable individual difference.

Female Longitudinal Anal Muscles or Conjoint Longitudinal Coats Extend into the Subcutaneous Tissue along the Vaginal Vestibule: A Histological Study Using Human Fetuses

Yusuke Kinugasa,Takashi Arakawa,Hiroshi Abe,Jose Francisco Rodríguez-Vázquez,Gen Murakami,Kenichi Sugihara 연세대학교의과대학 2013 Yonsei medical journal Vol.54 No.3

Purpose: It is still unclear whether the longitudinal anal muscles or conjoint longitudinal coats (CLCs) are attached to the vagina, although such an attachment, if present, would appear to make an important contribution to the integrated supportive system of the female pelvic floor. Materials and Methods: Using immunohistochemistry for smooth muscle actin, we examined semiserial frontal sections of 1) eleven female late-stage fetuses at 28-37 weeks of gestation, 2) two female middle-stage fetus (2 specimens at 13 weeks), and, 3) six male fetuses at 12 and 37 weeks as a comparison of the morphology. Results: In late-stage female fetuses, the CLCs consistently (11/11) extended into the subcutaneous tissue along the vaginal vestibule on the anterior side of the external anal sphincter. Lateral to the CLCs, the external anal sphincter also extended anteriorly toward the vaginal side walls. The anterior part of the CLCs originated from the perimysium of the levator ani muscle without any contribution of the rectal longitudinal muscle layer. However, in 2 female middle-stage fetuses, smooth muscles along the vestibulum extended superiorly toward the levetor ani sling. In male fetuses, the CLCs were separated from another subcutaneous smooth muscle along the scrotal raphe (posterior parts of the dartos layer) by fatty tissue. Conclusion: In terms of topographical anatomy, the female anterior CLCs are likely to correspond to the lateral extension of the perineal body (a bulky subcutaneous smooth muscle mass present in adult women), supporting the vaginal vestibule by transmission of force from the levator ani.

Distal vaginal atresia: a report of a rare type found a late-term fetus and its histological comparison with the normal pelvis

Ji Hyun Kim,Zhe Wu Jin,Hiroshi Abe,Gen Murakami,José Francisco Rodríguez Vázquez,Nobuyuki Hinata 대한해부학회 2022 Anatomy & Cell Biology Vol.55 No.4

Solitary distal vaginal atresia is generally caused by a transverse septum or an imperforate hymen. We found a novel type of distal vaginal atresia in a late-term fetus (gestational age approximately 28 weeks) in our histology collection. This fetus had a vaginal vestibule that was closed and covered by a thick subcutaneous tissue beneath the perineal skin in the immediately inferior or superficial side of the imperforate hymen. The uterus, uterine tube, anus, and anal canal had normal development. The urethral rhabdosphincters were well-developed and had a normal topographical relationship with the vagina, but the urethrovaginal sphincter was absent. Thus, vaginal descent seemed to occur normally and form the vestibule. However, the external orifice of the urethra consisted of a highly folded duct with hypertrophied squamous epithelium. Notably, the corpus cavernosum and crus of the clitoris had poor development and were embedded in the subcutaneous tissue, distant from the vestibule. Normally, the cloacal membrane shifts from the bottom of the urogenital sinus to the inferior aspect of the thick and elongated genital tubercle after establishment of the urorectal septum. Therefore, we speculate there was a failure in the transposition of the cloacal membrane caused by decreased elongation of the genital tubercle. The histology of this anomaly strongly suggested that the hymen does not represent a part of the cloacal membrane, but is instead a product that appears during the late recanalization of the distal vagina after vaginal descent. The transverse septum was also likely to form during this recanalization.