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        Changing Trends in Surgery for Retinal Detachment in Korea

        조가은,김성욱,강세웅,Korean Retina Society 대한안과학회 2014 Korean Journal of Ophthalmology Vol.28 No.6

        Purpose: To analyze trends in rhegmatogenous retinal detachment (RRD) surgery among the members of theKorean Retina Society from 2001 to 2013. Methods: In 2013, surveys were conducted by email and post to investigate the current practice patterns regardingRRD treatment. Questions included how surgeons would manage six cases of hypothetical RRD. Resultswere compared to those reported in 2001. Results: A total of 133 members (60.7%) in 2013 and 46 members(79.3%) in 2001 responded to the survey. Preference for pneumatic retinopexy has decreased in uncomplicated primary RRD (p = 0.004). More respondentsin 2013 selected vitrectomy as the primary procedure when mild vitreous hemorrhage (p = 0.001),myopia (p = 0.044) and history of successful scleral buckling on the fellow eye (p = 0.044) were added to theprimary scenario. Vitrectomy was over twice as popular in cases of pseudophakic, macula-off RRD with posteriorcapsular opacity (p = 0.001). Conclusions: For RRD with myopia, pseudophakia and media opacity, surgical interventions over the last decadehave drastically shifted from scleral buckling and pneumatic retinopexy to vitrectomy.

      • Expression of a Functional Anti-Cucumber Mosaic Virus Single-Chain Variable Fragment Antibody in Tobacco Plants (Nacotiana tabacum)

        Heng Chua Kek,Khalid Norzulaani,Othman Retina Yasmin The Korean Society of Plant Biotechnology 2006 Plant molecular biology and biotechnology research Vol.8 No.1

        As an alternative method to produce low cost reagents for immunodiagnosis and protect the plants from viral disease, a gene encoding a single chain variable fragment(scFv) recombinant antibody targeted to the coat protein of cucumber mosaic virus (CMV) was expressed in Nacotiana tabacum. The source of the scFv recombinant antibody gene was from spleen tissue of an immunized mouse. The gene was initially cloned into the pCANTAB5E phagemid and expressed in E. coli. In the following study, the antibody gene was subcloned into the plant expression vector, pCAMBIA-1301 and introduced into tobacco leaf tissue via Agrobacterium tumefacients mediated transformation. After transformation, 56 out of 58 plants were shown to carry the desired anti-CMV scFv gene by PCR analysis. Overall, only 12.5% of the 56 putative transgenic plants were found to express the antibody to a detectable level.

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