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Genoprotective Effects of the Green Tea-Derived Polyphenol/Epicatechin Gallate in C6 Astroglial
Renata T. Abib,Andre´ Quincozes-Santos,Caroline Zanotto,Fares Zeida´n-Chulia,Paula S. Lunardi,Carlos-Alberto Gonc¸alves,Carmem Gottfried 한국식품영양과학회 2010 Journal of medicinal food Vol.13 No.5
In vitro and in vivo studies have recently reported significant chemopreventive effects of green tea-derived polyphenols in different diseases. However, it remains unclear how such effects could be triggered. In order to elucidate the effects of epicatechin gallate (ECG) in C6 cells, both by itself and against H2O2-induced genotoxicity, measurements of DNA strand breaks and chromosome loss were performed. DNA damage was measured by comet and micronucleus assays. The present study shows for the first time how ECG, the major green tea-derived polyphenol, is able to exert dose-dependent genoprotective effects in an H2O2-induced toxicity model of C6 astroglial cells. We demonstrate that doses of ECG in a range from 0.1 to 1μM were able to completely prevent H2O2-induced genotoxicity in vitro. In contrast, considerably higher concentrations of ECG (10μM) were able to reverse previous positive effects in a dose- and time-dependent manner. The same results were confirmed by both comet (F3,9=336,148; P<.001) and micronucleus (F3,9=23,228; P<.001) methods. Together, our data show ECG as a dose-dependent genoprotective compound in C6 astroglial cells. This indicates that small doses of polyphenols included in our diet could have beneficial effects on neural cells, contributing to prevention of oxidative stress-associated brain pathologies. In addition, our data highlight the importance of strictly modulating doses and/or consumption of antioxidant-fortified foods or additional supplements containing such beneficial molecules.
Growth performance, carcass and meat quality of lambs supplemented different vegetable oils
Renata Miltko,Malgorzata Paulina Majewska,Grzegorz Belzecki,Katarzyna Kula,Barbara Kowalik 아세아·태평양축산학회 2019 Animal Bioscience Vol.32 No.6
Objective: The purpose of this study was to evaluate the effect of rapeseed and linseed oil supplementations on performance and meat quality of lambs. Methods: The experiment was conducted on 18 growing (100-day-old) lambs of 19.7±1.9 kg live weight, assigned to 3 groups of 6 animals each. Control lambs were fed meadow hay and concentrate alone. Experimental animals additionally received rapeseed or linseed oils at a dose of 50 g/d. The lambs were slaughtered at an average body weight of 35.7±0.5 kg. Results: The dressing percentage was higher in lambs fed rapeseed oil. Total saturated fatty acids (SFA) and C15:0, C16:0, C17:0, C21:0, C24:0 were lower in longissimus dorsi muscle (MLD) in lambs fed linseed oil. Supplementation of diet with linseed oil decreased concentrations of total monounsaturated fatty acids and C16:1, C17:1, C18:1 cis-9 in MLD. The concentrations of n-3 polyunsaturated fatty acids (PUFA) and C18:3 n-3, C20:5 n-3 in MLD were higher in lambs fed linseed oil than in other groups. Oils supplementation to diets resulted in increased concentration of C22:6 n-3 in MLD. The inclusion of linseed oil into the diet increased the contents of total PUFA, n-3 PUFA and C18:3 n-3, C20:5 n-3, C22:6 n-3 in semitendinosus muscle in comparison to control. A tendency towards a lower n:6/n:3 ratio in MLD was observed when lambs were supplemented linseed oil. Conclusion: The supplementation of linseed oil to diets seems to reduce the concentration of SFA and increase the concentration of n-3 PUFA. The n-6/n-3 ratio is an important nutritional factor, and its value has been favorably decreased below 2, thereby achieving an important target related to human health. Due to these changes carcass fatty acid profile was improved, and so enhanced lamb meat healthy propertie
Palm Oil Plantations : Nowhere to Escape
Renata Sandhi LIBRASWULAN Asian Center for Women's Studies : Ewha Womans Uni 2014 Asian Journal of Women's Studies(AJWS) Vol.20 No.4
Although palm oil plantations have been there in Indonesia for a century, the laborers who work on them have seen insignificant change compared to workers in other sectors, especially in industry. As of the colonial era, growers have imposed huge work targets and fines. They have also engaged in arbitrary treatment of labor and imposed unfair wage systems. These practices have led to social and environmental conflicts and greatly impoverished the plantation workers. Instead of providing assistance to them, the government perpetuates the company practices by failing to impose regulations to protect plantation laborers. This has also impacted the lives of women and children adversely. Poverty often forces generations of families to work on the plantations. The welfare of plantation workers needs to be addressed in order to empower them to reclaim their rights as labor and as human beings. Meskipun perkebunan kelapa sawit di Indonesia telah berumur 100 tahun, namun peningkatan kualitas hidup buruh perkebunan tidak signifikan dan sejalan dengan kemajuan yang telah dicapai oleh sector lainnya terutama sector industry. Sejak era kolonialisme, buruh ditempatkan didalam perkebunan oleh pengusaha perkebunan kelapa sawit dan diberikan target kerja yang tinggi, membayar denda-denda, perlakuan sewenang-wenang dan pengusaha menerapkan sistem upah yang tidak adil. Praktik-praktik ini menyebabkan konflik sosial-lingkungan dan telah memiskinkan masyarakat buruh perkebunan. Pemerintah juga tidak menunjukan keberpihakannya kepada buruh perkebunan dengan gagal melahirkan undang-undang untuk melindungi buruh perkebunan. Hal ini berdampak negatif juga pada kehidupan perempuan dan anak-anak. Kemiskinan bahkan memaksa keturunan buruh untuk bekerja kembali di perkebunan sebagai buruh. Kesejahteraan buruh perkebunan perlu diperhatikan untuk memberdayakan buruh perkebunan sehingga mereka mampu untuk menuntut hak-haknya sebagai buruh dan mahluk hidup.
( Renata Vinholes Oliveira Da Rocha ),( Maria Isabel Morgan Martins ),( Flavia Tasmim Techera Antunes ),( Marcia Gerhardt Martins ),( Adriane Belló Klein ),( Dione Silva Corrêa ),( Alessandra Hubner D 대한폐경학회 2021 대한폐경학회지 Vol.27 No.3
Objectives: Menopause induces changes in neuronal transmission, leading to anxiety and depression. Changes in the brain’s glutamate levels cause psychological behavior in postmenopausal women. Omega-3 has been studied to improve some of these behaviors. Methods: Twenty-four female Wistar rats were divided into four groups: sham-operated treated with water (SO-W), sham-operated treated with omega-3 (SO-O), ovariectomized (OVX) treated with water (OVX-W), and bilateral OVX treated with omega-3 (OVX-O). These treatments were performed for 20 days via gavage, before and after surgery, totaling 40 days. Results: In the forced swimming, elevated plus-maze, and open field tests to assess behaviors, such as depression and anxiety, omega-3 improved these behaviors in both treated groups. The levels of thiobarbituric acid reactive substances (TBARS) in the brain were not different between the groups; however, there was a significant decrease in the catalase activity in the SO-O group compared with the SO-W group (P < 0.05). The glutamate level in the cerebrospinal fluid (CSF) was elevated in the SO-O group (P < 0.001) but not in the OVX-W or OVX-O groups. Conclusions: These results bring novel data when related to the glutamatergic system in the SO-O group. This has suggested that the action mechanism of omega-3 was not dependent on glutamate levels in the CSF of the OVX group, but it played a regulatory role in the sham-operated animals. To confirm this, more studies are needed to explore this field when relating to the estrogen and glutamate receptor changes in specific brain regions.
Functional activities of the Tsh protein from avian pathogenic Escherichia coli (APEC) strains
Renata K. T. Kobayashi,Luis Carlos J. Gaziri,Marilda C. Vidotto 대한수의학회 2010 Journal of Veterinary Science Vol.11 No.4
The temperature-sensitive hemagglutinin (Tsh) expressed by strains of avian pathogenic Escherichia (E.) coli (APEC) has both agglutinin and protease activities. Tsh is synthesized as a 140 kDa precursor protein, whose processing results in a 106 kDa passenger domain (Tshs) and a 33 kDa β-domain (Tshβ). In this study, both recombinant Tsh (rTsh) and supernatants from APEC, which contain Tshs (106 kDa), caused proteolysis of chicken tracheal mucin. Both rTsh (140 kDa) and pellets from wild-type APEC, which contain Tshβ (33 kDa), agglutinated chicken erythrocytes. On Western blots, the anti-rTsh antibody recognized the rTsh and 106 kDa proteins in recombinant E. coli BL21/pET 101-Tsh and in the supernatants from APEC grown at either 37oC or 42oC. Anti-rTsh also recognized a 33 kDa protein in the pellets from APEC13 cultures grown in either Luria-Bertani agar, colonization factor antigen agar, or mucin agar at either 26oC, 37oC, or 42oC, and in the extracts of outer membrane proteins of APEC. The 106 kDa protein was more evident when the bacteria were grown at 37oC in mucin agar, and it was not detected when the bacteria were grown at 26oC in any of the culture media used in this study. Chicken anti-Tsh serum inhibited hemagglutinating and mucinolytic activities of strain APEC13 and recombinant E. coli BL21/pET101-Tsh. This work suggests that the mucinolytic activity of Tsh might be important for the colonization of the avian tracheal mucous environment by APEC.
Renata M. Martinez,Daniela T. Longhi-Balbinot,Ana C. Zarpelon,Larissa Staurengo-Ferrari,Marcela M. Baracat,Sandra R. Georgetti,Roge´rio C. Sassonia,Waldiceu A. Verri Jr.,Rubia Casagrande 대한약학회 2015 Archives of Pharmacal Research Vol.38 No.4
We have recently developed betalain-richbeetroot (Beta vulgaris) dye (betalain) to be used in foodproducts. Betalain (30–300 mg/kg) intraperitoneal (i.p.)treatment diminished carrageenan (100 lg/paw)-inducedpaw edema and neutrophil migration to the paw skin tissue. Betalain (100 mg/kg) treatment by subcutaneous or peroral routes also inhibited the carrageenan-induced pawedema. Importantly, the post-treatment with betalain(100 mg/kg, i.p.) significantly inhibited carrageenan- andcomplete Freund’s adjuvant (10 ll/paw)-induced pawedema. Betalain (100 mg/kg) also reduced carrageenan(500 lg/cavity)-induced recruitment of total leukocytes,including mononuclear cells and neutrophils, as well asincreasing vascular permeability in the peritoneal cavity. Furthermore, betalain significantly reduced carrageenaninducedsuperoxide anion, tumor necrosis factor-alpha(TNF-a) and interleukin (IL)-1b levels in the peritonealfluid, as well as augmenting IL-10 levels. Therefore, thiscompound presents prominent anti-inflammatory effect oncarrageenan-induced paw edema and peritonitis by reducingthe production of superoxide anion and the cytokinesTNF-a and IL-1b, in addition to increasing IL-10 levels. These results suggest that betalain shows therapeuticpotential that could be utilized in the treatment of inflammation-associated diseases.