Significance of p16 Positive Expression in Oropharyngeal Cancers

Bixofis, Regiane B.,Sassi, Laurindo Moacir,Patussi, Cleverson,Jung, Juliana E.,Ioshii, Sergio O.,Schussel, Juliana L. Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.23

Background: The involvement of HPV in oral and oropharyngeal carcinogenesis was first proposed in 2004, based on epithelial HPV tropism and detection of HPV genotypes in oral squamous cell carcinoma samples. While 60-70% of oropharynx tumors may be HPV-positive, only 10 to 19% of tumors of the oral cavity, larynx and hypopharynx appear to have HPV infection. The aim of the study was to evaluate HPV infection associated with oropharyngeal cancer. Materials and Methods: Seventy-eight cases were selected for p16 immunoexpression reactions, and demographic data were collected for comparisons. Results: Most patients were over 60 years old, and 64.1% were smokers. Immunohistochemistry results showed that 86.3% of cases stained positive for p16 protein. Conclusion: The oropharyngeal cancer profile at Erasto Gaertner Hospital presented a high index of smokers over 60 years as well a high number of p16+ tumors, for what we can not determinate the main etiologic factor, but can be aware of the number of patients that presented HPV infection. Since prevention is still the best way to deal with cancer disease, it is important to analyze the interaction of these two etiologic factors and how to detect lesions at an early stage.

Numerical Methodology to Predict and Analyze Cavitating Flows in a Kaplan Turbine

Flavia Turi,Regiane Fortes-Patella,Guillaume Balarac 한국유체기계학회 2019 International journal of fluid machinery and syste Vol.12 No.4

A computational methodology to predict the cavitation phenomena appearance and evolution in a 5-blades Kaplan turbine scale model was developed. Two different inlet boundary conditions have been tested in both non-cavitating and cavitating regimes: the classical one, the mass flow rate and the new one, the total pressure. The best results were obtained applying a constant total pressure on the inlet. The torque and efficiency drop curves were well-predicted with the proposed calculation methodology and the numerical cavitation structures agreed with experimental observations. Indeed, this new inlet boundary condition allows to keep the machine head constant during the cavitation drop, as in experiments. Unsteady simulations are under investigation to improve the prediction and the analyses of more developed cavitating regimes.

Experimental and Numerical Investigation of the Erosive Potential of a Leading Edge Cavity

Jean-Bastien Carrat,Regiane Fortes-Patella,Jean-Pierre Franc 한국유체기계학회 2019 International journal of fluid machinery and syste Vol.12 No.2

This paper presents a joint experimental and numerical analysis of the erosive potential of an unsteady cavity that develops at the leading edge of a two-dimensional hydrofoil and periodically sheds vapour clouds. From an experimental viewpoint, the erosive potential was characterized by pressure pulse height spectra. The hydrofoil was equipped with eight pressure sensors made of PVDF piezoelectric film that allowed the measurement of flow aggressiveness at different locations along the hydrofoil chord. It was shown that the mean peak rate over a large number of cavity pulsations exhibits a maximum at a distance from the leading edge close to the maximum cavity length. Moreover, the increase in flow aggressiveness caused by an increase in flow velocity can be explained by an increase in both amplitude and frequency of impact loads. From a numerical viewpoint, the unsteady Reynolds averaged Navier-Stokes (RANS) equations were solved using a modified k-ε RNG turbulence model together with a homogeneous cavitation model within a two-dimensional approach. Flow aggressiveness was estimated from the Lagrangian derivative of the computed void fraction that allows identifying the regions of collapse of vapour structures. Three different critical regions from an erosive viewpoint were numerically identified. Apart from the region of collapse of the shed cloud (which was not instrumented in the present study), the computations showed a maximum of aggressiveness around the maximum cavity length as found experimentally. Another region of high aggressiveness closer to the leading edge and associated to the upward movement of the re-entrant jet was predicted by the present numerical model but not confirmed experimentally, which probably shows the limitation of a two-dimensional approach.

Numerical Cavitation Intensity on a Hydrofoil for 3D Homogeneous Unsteady Viscous Flows

Christophe Leclercq,Antoine Archer,Regiane Fortes-Patella,Fabien Cerru 한국유체기계학회 2017 International journal of fluid machinery and syste Vol.10 No.3

The cavitation erosion remains an industrial issue for many applications. This paper deals with the cavitation intensity, which can be described as the fluid mechanical loading leading to cavitation damage. The estimation of this quantity is a challenging problem both in terms of modeling the cavitating flow and predicting the erosion due to cavitation. For this purpose, a numerical methodology was proposed to estimate cavitation intensity from 3D unsteady cavitating flow simulations. CFD calculations were carried out using Code_Saturne, which enables U-RANS equations resolution for a homogeneous fluid mixture using the Merkle"s model, coupled to a - turbulence model with the Reboud"s correction. A post-process cavitation intensity prediction model was developed based on pressure and void fraction derivatives. This model is applied on a flow around a hydrofoil using different physical (inlet velocities) and numerical (meshes and time steps) parameters. The article presents the cavitation intensity model as well as the comparison of this model with experimental results. The numerical predictions of cavitation damage are in good agreement with experimental results obtained by pitting test.

Numerical Cavitation Intensity on a Hydrofoil for 3D Homogeneous Unsteady Viscous Flows

Leclercq, Christophe,Archer, Antoine,Fortes-Patella, Regiane,Cerru, Fabien Korean Society for Fluid machinery 2017 International journal of fluid machinery and syste Vol.10 No.3

The cavitation erosion remains an industrial issue for many applications. This paper deals with the cavitation intensity, which can be described as the fluid mechanical loading leading to cavitation damage. The estimation of this quantity is a challenging problem both in terms of modeling the cavitating flow and predicting the erosion due to cavitation. For this purpose, a numerical methodology was proposed to estimate cavitation intensity from 3D unsteady cavitating flow simulations. CFD calculations were carried out using Code_Saturne, which enables U-RANS equations resolution for a homogeneous fluid mixture using the Merkle's model, coupled to a $k-{\varepsilon}$ turbulence model with the Reboud's correction. A post-process cavitation intensity prediction model was developed based on pressure and void fraction derivatives. This model is applied on a flow around a hydrofoil using different physical (inlet velocities) and numerical (meshes and time steps) parameters. The article presents the cavitation intensity model as well as the comparison of this model with experimental results. The numerical predictions of cavitation damage are in good agreement with experimental results obtained by pitting test.

The effect of repeated surface treatment of zirconia on its bond strength to resin cement

Lucas Campagnaro Maciel,Marina Amaral,Daher Antonio Queiroz,Kusai Baroudi,Laís Regiane Silva-Concílio 대한치과보철학회 2020 The Journal of Advanced Prosthodontics Vol.12 No.5

PURPOSE. The aim of this study is to evaluate the influence of repeated surface treatments on wettability and surface roughness for zirconia surface and bond strength of zirconia-based ceramics to resin cement. MATERIALS AND METHODS. Seventy blocks (10 × 10 × 3 mm) of zirconia-based ceramics were fabricated and divided into two groups according to the surface treatments: (A) 110 μm Al2O3 airborne-particle abrasion and (R) 110 μm silica modified Al2O3 airborne-particle abrasion. At stage 2, each group was subdivided into 5 groups according to the surface retreatments: (a) 110 μm Al2O3 airborne-particle abrasion, (r) 110 μm silica modified Al2O3 airborne-particle abrasion, (D) diamond bur, (Da) diamond bur + 110 μm Al2O3 airborne-particle abrasion, and (Dr) diamond bur + 110 μm silica modified Al2O3 airborne-particle abrasion. Cylinders of self-adhesive resin cement were cemented onto each treated ceramic surface and subjected to micro-shear bond strength test. Additional specimens were prepared for roughness and wettability analyses. The data were subjected to t-test and One-way ANOVA followed by Tukey’s post hoc test (α=.05). RESULTS. At stage 1, group R presented higher bond strength values than group A (P=.000). There was a statistically significant increase of bond strength at stage 2 for group A (P=.003). The diamond bur influenced the surface roughness, increasing the values (P=.023). Group R provided better wettability. Regardless of the applied surface treatment, most of failures were adhesive. CONCLUSION. The combination of application and reapplication of Rocatec Plus showed the best results of bond strength. Surface retreatment and recementation might be an indicated clinical strategy.

Influence of polishing systems on roughness and color change of two dental ceramics

Lucas Campagnaro Maciel,Carlos Frederico Bettcher Silva,Ricardo Huver de Jesus,Lais Regiane da Silva Concilio,Stefania Carvalho Kano,Anuar Antonio Xible 대한치과보철학회 2019 The Journal of Advanced Prosthodontics Vol.11 No.4

PURPOSE. To evaluate the polishing effect on roughness and color change of pressed and layering ceramics after immersion in coffee solution. MATERIALS AND METHODS. 88 ceramic discs (1.0 mm × 10.0 mm) were manufactured - 44 nano-fluorapatite layering ceramics (IPS e.max Ceram. Group C) and 44 pressed lithium disilicate ceramic discs (IPS e. max Press - Group P). Each group was divided into 4 subgroups according to surface treatments: (G) Glaze, (S) Shofu polishing system (Shofu Inc.), (E) Edenta AG polishing System, (KG) 30-μm diamond granulation tip. Surface roughness (Ra) and color change (ΔE) measurings after the surface treatments were performed, before and 12 days after the immersion in coffee solution. A samples’ qualitative analysis was conducted with a scanning electron microscopy (SEM). Data were statistically-treated with oneway-ANOVA and Duncan’s tests, apart from paired t-test and Pearson’s correlation test (α=5%). RESULTS. The decrescent order, both for surface roughness (Ra) and ΔE for both ceramics were: KG > E > S > G (P<.05). With exception for PG and CG subgroups, which did not present statistical difference between them, all other pressed ceramics subgroups presented smaller Ra values and greater ΔE values than the layering ceramics subgroups (P<.05). CONCLUSION. Although mechanical polishing systems presented intermediate Ra values, their colors were considered clinically acceptable. There is a strong correlation between the surface roughness and the color change of tested ceramics.

Effect of polymerization method and fabrication method on occlusal vertical dimension and occlusal contacts of complete-arch prosthesis

Ana Paula Barbosa Lima,Rafael Pino Vitti,Marina Amaral,Ana Christina Claro Neves,Lais Regiane da Silva Concilio 대한치과보철학회 2018 The Journal of Advanced Prosthodontics Vol.10 No.2

PURPOSE. This study evaluated the dimensional stability of a complete-arch prosthesis processed by conventional method in water bath or microwave energy and polymerized by two different curing cycles. MATERIALS AND METHODS. Forty maxillary complete-arch prostheses were randomly divided into four groups (n = 10): MW1 - acrylic resin cured by one microwave cycle; MW2 - acrylic resin cured by two microwave cycles: WB1 - conventional acrylic resin polymerized using one curing cycle in a water bath; WB2 - conventional acrylic resin polymerized using two curing cycles in a water bath. For evaluation of dimensional stability, occlusal vertical dimension (OVD) and area of contact points were measured in two different measurement times: before and after the polymerization method. A digital caliper was used for OVD measurement. Occlusal contact registration strips were used between maxillary and mandibular dentures to measure the contact points. The images were measured using the software IpWin32, and the differences before and after the polymerization methods were calculated. The data were statistically analyzed using the one-way ANOVA and Tukey test (α = .05). RESULTS. The results demonstrated significant statistical differences for OVD between different measurement times for all groups. MW1 presented the highest OVD values, while WB2 had the lowest OVD values (P<.05). No statistical differences were found for area of contact points among the groups (P=.7150). CONCLUSION. The conventional acrylic resin polymerized using two curing cycles in a water bath led to less difference in OVD of complete-arch prosthesis.

Adhesive systems applied to dentin substrate under electric current; systematic review

Maciel Carolina Menezes,Souto Tatiane Cristina Vieira,Pinto Bárbara de Almeida,Silva-Concilio Laís Regiane,Baroudi Kusai,Vitti Rafael Pino 대한치과보존학회 2021 Restorative Dentistry & Endodontics Vol.46 No.4

Objectives The purpose of this systematic review was to collect and discuss the technique of adhesive systems application on dentin substrate under electric current. Materials and Methods The first search strategy was based on data available at PubMed, LILACS, Scielo, Scopus, and Cochrane Library, using a combination of descriptors such as “dentin bond agents OR adhesive system AND electric current OR electrobond” or “dentin bonding agents OR dentin bonding agent application OR adhesive system AND electric current OR electrobond”, with no limit regarding the publication year. The second search strategy was based on the articles' references found previously. An additional search strategy was applied that concerned the proposed theme in the SBU-UNICAMP (Unicamp's Library System Institutional Repository). Results Twelve studies published between 2006 and 2020 were found. The analyses of the selected studies showed that the use of electric current during adhesive systems application on dentin, whether conventional or self-conditioning, increases resinous monomer infiltration in the dentin substrate, which improves the hybridization processes and the bond strength of the restorative material to dentin. Conclusions Despite the favorable results related to the use of this technique, there is still no specific protocol for the application of adhesive systems under electric current. Objectives The purpose of this systematic review was to collect and discuss the technique of adhesive systems application on dentin substrate under electric current. Materials and Methods The first search strategy was based on data available at PubMed, LILACS, Scielo, Scopus, and Cochrane Library, using a combination of descriptors such as “dentin bond agents OR adhesive system AND electric current OR electrobond” or “dentin bonding agents OR dentin bonding agent application OR adhesive system AND electric current OR electrobond”, with no limit regarding the publication year. The second search strategy was based on the articles' references found previously. An additional search strategy was applied that concerned the proposed theme in the SBU-UNICAMP (Unicamp's Library System Institutional Repository). Results Twelve studies published between 2006 and 2020 were found. The analyses of the selected studies showed that the use of electric current during adhesive systems application on dentin, whether conventional or self-conditioning, increases resinous monomer infiltration in the dentin substrate, which improves the hybridization processes and the bond strength of the restorative material to dentin. Conclusions Despite the favorable results related to the use of this technique, there is still no specific protocol for the application of adhesive systems under electric current.