Comparison of Ginsenoside Components of Jinan Red Ginseng Products for Quality Certification

Soo Chang Na(나수창),Re Jeong Jeon(전례정),Ji Won Lee(이지원),Chung Berm Park(박충범),Bo Hee Park(박보희) 한국약용작물학회 2019 한국약용작물학회 학술대회논문집 Vol.2019 No.2

Factors predicting radiation pneumonitis in locally advanced non-small cell lung cancer

Kim, Myung-Soo,Lee, Ji-Hae,Ha, Bo-Ram,Lee, Re-Na,Lee, Kyung-Ja,Suh, Hyun-Suk The Korean Society for Radiation Oncology 2011 Radiation Oncology Journal Vol.29 No.3

Purpose: Thoracic radiotherapy is a major treatment modality of stage III non-small cell lung cancer. The normal lung tissue is sensitive to radiation and radiation pneumonitis is the most important dose-limiting complication of thoracic radiation therapy. This study was performed to identify the clinical and dosimetric parameters related to the risk of radiation pneumonitis after definitive radiotherapy in stage III non-small cell cancer patients. Materials and Methods: The medical records were reviewed for 49 patients who completed definitive radiation therapy for locally advanced non-small cell lung cancer from August 2000 to February 2010. Radiation therapy was delivered with the daily dose of 1.8 Gy to 2.0 Gy and the total radiation dose ranged from 50.0 Gy to 70.2 Gy (median, 61.2 Gy). Elective nodal irradiation was delivered at a dose of 45.0 Gy to 50.0 Gy. Seven patients (14.3%) were treated with radiation therapy alone and forty two patients (85.7%) were treated with chemotherapy either sequentially or concurrently. Results: Twenty-five cases (51.0%) out of 49 cases experienced radiation pneumonitis. According to the radiation pneumonitis grade, 10 (20.4%) were grade 1, 9 (18.4%) were grade 2, 4 (8.2%) were grade 3, and 2 (4.1%) were grade 4. In the univariate analyses, no clinical factors including age, sex, performance status, smoking history, underlying lung disease, tumor location, total radiation dose and chemotherapy were associated with grade ${\geq}2$ radiation pneumonitis. In the subgroup analysis of the chemotherapy group, concurrent rather than sequential chemotherapy was significantly related to grade ${\geq}2$ radiation pneumonitis comparing sequential chemotherapy. In the univariate analysis with dosimetric factors, mean lung dose (MLD), $V_{20}$, $V_{30}$, $V_{40}$, MLDipsi, $V_{20}$ipsi, $V_{30}$ipsi, and $V_{40}$ipsi were associated with grade ${\geq}2$ radiation pneumonitis. In addition, multivariate analysis showed that MLD and V30 were independent predicting factors for grade ${\geq}2$ radiation pneumonitis. Conclusion: Concurrent chemotherapy, MLD and $V_{30}$ were statistically significant predictors of grade ${\geq}2$ radiation pneumonitis in patients with stage III non-small cell lung cancer undergoing definitive radiotherapy. The cutoff values for MLD and $V_{30}$ were 16 Gy and 18%, respectively.

Human umbilical cord blood mesenchymal stem cells engineered to overexpress growth factors accelerate outcomes in hair growth

Dong Ho Bak,Mi Ji Choi,Soon Re Kim,Byung Chul Lee,Jae Min Kim,Eun Su Jeon,Wonil Oh,Ee Seok Lim,Byung Cheol Park,Moo Joong Kim,Jungtae Na,Beom Joon Kim 대한약리학회 2018 The Korean Journal of Physiology & Pharmacology Vol.22 No.5

Human umbilical cord blood mesenchymal stem cells (hUCB-MSCs) are used in tissue repair and regeneration; however, the mechanisms involved are not well understood. We investigated the hair growth-promoting effects of hUCB-MSCs treatment to determine whether hUCB-MSCs enhance the promotion of hair growth. Furthermore, we attempted to identify the factors responsible for hair growth. The effects of hUCB-MSCs on hair growth were investigated in vivo, and hUCB-MSCs advanced anagen onset and hair follicle neogeneration. We found that hUCB-MSCs coculture increased the viability and up-regulated hair induction-related proteins of human dermal papilla cells (hDPCs) in vitro. A growth factor antibody array revealed that secretory factors from hUCB-MSCs are related to hair growth. Insulin-like growth factor binding protein-1 (IGFBP-1) and vascular endothelial growth factor (VEGF) were increased in co-culture medium. Finally, we found that IGFBP-1, through the colocalization of an IGF-1 and IGFBP-1, had positive effects on cell viability; VEGF secretion; expression of alkaline phosphatase (ALP), CD133, and β-catenin; and formation of hDPCs 3D spheroids. Taken together, these data suggest that hUCB-MSCs promote hair growth via a paracrine mechanism.

Factors Associated with the Discrepancy between Exercise Capacity and Airflow Limitation in Patients with Chronic Obstructive Pulmonary Disease

Ho Cheol Kim,Tae Hoon Kim,I Re Heo,Na Young Kim,Joo Hun Park,Hee-Young Yoon,Ji Ye Jung,Seung Won Ra,Ki-Suck Jung,Kwang Ha Yoo 대한결핵및호흡기학회 2024 Tuberculosis and Respiratory Diseases Vol.87 No.2

Background: Exercise capacity is associated with lung function decline in chronicobstructive pulmonary disease (COPD) patients, but a discrepancy between exercisecapacity and airflow limitation exists. This study aimed to explore factors contributingto this discrepancy in COPD patients. Methods: Data for this prospective study were obtained from the Korean COPD SubgroupStudy. The exercise capacity and airflow limitation were assessed using the6-minute walk distance (6-MWD; m) and forced expiratory volume in 1 second (FEV1). Participants were divided into four groups: FEV1 >50%+6-MWD >350, FEV1 >50%+6-MWD ≤350, FEV1 ≤50%+6-MWD >350, and FEV1 ≤50%+6-MWD ≤350 and their clinicalcharacteristics were compared. Results: A total of 883 patients (male:female, 822:61; mean age, 68.3±7.97 years) wereenrolled. Among 591 patients with FEV1 >50%, 242 were in the 6-MWD ≤350 group, andamong 292 patients with FEV1 ≤50%, 185 were in the 6-MWD >350 group. The multipleregression analyses revealed that male sex (odds ratio [OR], 8.779; 95% confidence interval[CI], 1.539 to 50.087; p=0.014), current smoking status (OR, 0.355; 95% CI, 0.178to 0.709; p=0.003), and hemoglobin levels (OR, 1.332; 95% CI, 1.077 to 1.648; p=0.008)were significantly associated with discrepancies in exercise capacity and airflow limitationin patients with FEV1 >50%. Meanwhile, in patients with FEV1 ≤50%, diffusioncapacity of carbon monoxide (OR, 0.945; 95% CI, 0.912 to 0.979; p=0.002) was significantlyassociated with discrepancies between exercise capacity and airflow limitation. Conclusion: The exercise capacity of COPD patients may be influenced by factors otherthan airflow limitation, so these aspects should be considered when assessing andtreating patients.

Human umbilical cord blood mesenchymal stem cells engineered to overexpress growth factors accelerate outcomes in hair growth

Bak, Dong Ho,Choi, Mi Ji,Kim, Soon Re,Lee, Byung Chul,Kim, Jae Min,Jeon, Eun Su,Oh, Wonil,Lim, Ee Seok,Park, Byung Cheol,Kim, Moo Joong,Na, Jungtae,Kim, Beom Joon The Korean Society of Pharmacology 2018 The Korean Journal of Physiology & Pharmacology Vol.22 No.5

Human umbilical cord blood mesenchymal stem cells (hUCB-MSCs) are used in tissue repair and regeneration; however, the mechanisms involved are not well understood. We investigated the hair growth-promoting effects of hUCB-MSCs treatment to determine whether hUCB-MSCs enhance the promotion of hair growth. Furthermore, we attempted to identify the factors responsible for hair growth. The effects of hUCB-MSCs on hair growth were investigated in vivo, and hUCB-MSCs advanced anagen onset and hair follicle neogeneration. We found that hUCB-MSCs co-culture increased the viability and up-regulated hair induction-related proteins of human dermal papilla cells (hDPCs) in vitro. A growth factor antibody array revealed that secretory factors from hUCB-MSCs are related to hair growth. Insulin-like growth factor binding protein-1 (IGFBP-1) and vascular endothelial growth factor (VEGF) were increased in co-culture medium. Finally, we found that IGFBP-1, through the co-localization of an IGF-1 and IGFBP-1, had positive effects on cell viability; VEGF secretion; expression of alkaline phosphatase (ALP), CD133, and ${\beta}-catenin$; and formation of hDPCs 3D spheroids. Taken together, these data suggest that hUCB-MSCs promote hair growth via a paracrine mechanism.

Analysis of Physical Properties for Various Compositions of Reusable LMG and LCV Micelle Gel

Kang, Jin Mook,Lee, Dong Han,Cho, Yu Ra,Hwang, Seon Bung,Ji, Young Hoon,Ahn, So Hyun,Keum, Ki Chang,Lee, Re Na,Cho, Sam Ju,Noh, Insup Korean Society of Medical Physics 2016 의학물리 Vol.27 No.4

In this study, we evaluated the reusable leuco malachite green (LMG) micelle gel properties dependent on various components of chemical concentration and compared with leuco crystal violet (LCV). The gels were delivered to 10, 20, 30, 40 and 50 Gy at 6 MV photon beam from linear accelerator and analyzed using spectrophotometry. We confirmed that the reusable LMG and LVC absorbance wavelength peak were made up at 630 nm and 600 nm respectively. The transparency of reusable LMG decreased with higher amount of trichloroacetic acid (TCAA) and lower reusable LMG dyes. 1 mM reusable LMG was the lowest transparency. The sensitivity was increased depending on lower trichloroacetic acid (TCAA) concentrations and the amount of suitable surfactant (Triton X-100), which was found to be 7 mM. However, we were not able to investigate sensitivity effects factor from reusable LMG dyes. The gel dosimeter containing 16 mM TCAA, 7 mM Triton X-100 gel dosimeter showed the highest sensitivity at $0.0021{\pm}0.0001cm^{-1}.Gy^{-1}$. The sensitivity of LCV was found to be higher than reusable LMG at $0.0037{\pm}0.0005cm^{-1}.Gy^{-1}$. The reusable LMG and LCV dose responses were shown to be $R^2=0.997$, $R^2=0.999$ respectively, as stable measurement results. Future research is necessary to improve dose sensitivity, dose rate dependency and gel fading with extensive chemical formulations.

CR 시스템을 이용한 방사선 종양학과의 Filmless 환경 구축

김동영,이지혜,김명수,하보람,이천희,김소영,안소현,이레나,Kim, Dong-Young,Lee, Ji-Hae,Kim, Myung-Soo,Ha, Bo-Ram,Lee, Cheon-Hee,Kim, So-Yeong,Ahn, So-Hyun,Lee, Re-Na 한국의학물리학회 2011 의학물리 Vol.22 No.3

The analog image based system consisted of a simulator and medical linear accelerator (LINAC) for radiotherapy was upgraded to digital medical image based system by exchanging the X-ray film with Computed Radiography (CR). With minimum equipments shift and similar treatment process, it was possible that the new digital image system was adapted by the users in short time. The film cassette and the film developer device were substituted with a CR cassette and a CR Reader, where the ViewBox was replaced with a small size PC and a monitor. The viewer software suitable for radiotherapy was developed to maximize the benefit of digital image, and as the result the convenience and the effectiveness was improved. It has two windows to display two different images in the same time and equipped various search capability, contouring, window leveling, image resizing, translation, rotation and registration functions. In order to avoid any discontinuance of the treatment while the transition to digital image, the film and the CR was used together for 1 week, and then the film developer was removed. Since then the CR System has been operated stably for 2 months, and the various requests from users have been reflected to improve the system. 방사선치료를 위한 아날로그영상기반의 시뮬레이터와 의료용 선형가속기로 이루어진 시스템을 X-ray 필름을 Computed Radiography (CR)로 교체하여 디지털영상기반의 시스템으로 개선하였다. 장비교체를 최소화하였고 기존의 치료과정과 유사하도록 하여 사용자들이 짧은 시간에 적응할 수 있도록 하였다. 필름카세트와 필름현상기는 CR카세트와 CR리더기로 대신하였고, ViewBox는 소형PC와 모니터로 대체되었다. 디지털영상의 장점을 충분히 활용하기 위해 방사선종양학과에 적합한 Viewer 소프트웨어를 개발하여 업무의 편리성과 효율성을 도모하였다. Viewer 소프트웨어는 2개의 이미지를 동시에 볼 수 있으며, 이들 영상의 비교도 가능하다. 다양한 검색기능과 Contouring 기능, 윈도우폭/레벨 조절기능을 갖추었다. 업무의 중단이 없도록 하기 위해 1주일간 기존 필름과 CR을 공동으로 운용하며 문제점을 수정한 후 필름시스템을 제거하고 CR시스템을 운용하였다. CR시스템은 안정적으로 운용되고 있으며, 본격적으로 CR시스템이 이용되면서 사용자로부터 제기되는 여러 요구사항을 반영하여 기능을 개선하였다.

Development of Ultrasound Phantom for Volume Calibration

김혜영,이지혜,이경자,서현숙,이레나,Kim, Hye-Young,Lee, Ji-Hae,Lee, Kyung-Ja,Suh, Hyun-Suk,Lee, Re-Na Korean Society of Medical Physics 2008 의학물리 Vol.19 No.4

본 연구에서는 2차원 초음파 시스템의 체적 측정 정확성을 측정하기 위한 초음파 팬텀을 설계 및 제작하였다. 팬텀은 체적 측정용 타겟과 백그라운드 물질로 구성되었다. 백그라운드 물질은 agarose gel과 물을 혼합하여 만들었다. 타켓은 모양과 부피를 변화시키기 위하여 신축성 있는 물질로 선택하였고 타겟에 물을 채운 후 백그라운드 물질에 삽입하였다. 2차원 초음파 시스템의 정확성을 평가하기 위하여 세가지 형태의 타겟(구형, 2가지 형태의 타원형, 삼각 기둥형)을 제작하였다. 타원형의 경우, 하나는 길이와 폭의 크기가 비슷하도록 제작하였고(타원 1) 다른 하나는 길이가 폭보다 2배 이상인 모양(타원 2)으로 제작하였다. 각각 형태의 타겟 부피는 94 cc에서 450 cc까지 변화하였고 2차원 초음파 장비(128XP, ACUSON)를 이용하여 정확성을 측정하였다. 구 모양의 타겟에 삽입된 물의 실제 체적과 측정된 체적 간의 차는 6.7에서 11% 이다. 타원형 타겟의 경우 타원 1은 9.2에서 10.5% 사이의 차이를 보이는데 타원 2의 경우는 25.7%의 오차가 있다. 삼각기둥 형태의 경우는 실제체적과 측정체적간 20.8에서 35%의 차이를 보였다. 쉽고 간편한 방법의 초음파 팬텀의 제작 방법을 소개하였고 제작된 팬텀을 이용할 경우 초음파 시스템의 부피 측정 정확성을 평가할 수 있음을 입증하였다. The purpose of this study was to design and construct an ultrasound phantom for volume calibration and evaluate the volume measurement accuracy of a 2 dimensional ultrasonic system. Ultrasound phantom was designed, constructed and tested. The phantom consisted of a background material and a target. The background was made by mixing agarose gel with water. A target, made with an elastic material, was filled with water to vary its volume and shape and inserted into background material. To evaluate accuracy of a 2 dimensional ultrasonic system (128XP, ACUSON), three different shapes of targets (a sphere, 2 ellipsoids and a triangular prism) were constructed. In case of ellipsoid shape, two targets, one with same size length and width (ellipsoid 1) and another with the length 2 times longer than width (ellipsoid 2) were examined. The target volumes of each shape were varied from 94cc to 450cc and measurement accuracy was examined. The volume difference between the real and measured target of the sphere shape ranged between 6.7 and 11%. For the ellipsoid targets, the differences ranged from 9.2 to 10.5% with ellipsoid 1 and 25.7% with ellipsoid 2. The volume difference of the triangular prism target ranged between 20.8 and 35%. An easy and simple method of constructing an ultrasound phantom was introduced and it was possible to check the volume measurement accuracy of an ultrasound system.

Historical control data from 13-week repeated toxicity studies in Crj : CD (SD) rats

Jung-Min Lee,Min-Ah Lee,Hyeon-Nam Do,Yun-Ik Song,Re-Ji-Na Bae,Hye-Yeong Lee,Sun Hee Park,Jin Seok Kang,Jong-koo Kang 한국실험동물학회 2012 Laboratory Animal Research Vol.28 No.2

Reference ranges of standard experimental parameters are useful for comparisons in toxicology. The aim of this study was to collect data from 13-week repeated toxicity studies in Crl:CD (SD) rats, a strain widely used for toxicity and efficacy research, for establishing domestic reference values. Data on body weight, food consumption; urinalysis, hematological, and blood biochemical parameters; and organ weights were collected from 11 toxicity studies in 220 Crl:CD (SD) rats (110 males and 110 females). The studies had been performed at a single testing facility over the last 5 years and involved animals sourced from a single breeder. The findings were collated as means, standard deviations, percentages, and ranges. Urine volume, uterus weight, eosinophil, and basophil counts, and triglyceride, total bilirubin, and gamma-glutamyl transpeptidase levels showed standard deviations of 30% or more. These historical control data would help to interpret the effects of test substances in routine toxicity and efficacy studies.

Historical Control Data from 4-week Repeated Toxicity Studies in Crj:CD (SD) Rats

Jung-Min Lee,Min-Ah Lee,Hyeon-Nam Do,Re-Ji-Na Bae,Mi Ju Lee,Myoung Jun Kim,Ho-Song Jang,Sun Hee Park,Hye-Yeong Lee,Jin Seok Kang,Jong-Koo Kang 대한의생명과학회 2012 Biomedical Science Letters Vol.18 No.3

Reference ranges of standard experimental parameters are useful for comparisons in toxicology. The aim of this study was to collect data from 4-week repeated toxicity studies in Crl:CD (SD) rats, a strain widely used for toxicity and efficacy research, for establishing domestic reference values. Data on body weight, food consumption; urinalysis, hematological, and blood biochemical parameters; and organ weights were collected from 16 toxicity studies in 220 Crl:CD (SD) rats (110 males and 110 females). The studies had been performed at a single testing facility over the last 3 years and involved animals sourced from a single breeder. The findings were collated as means, standard deviations, percentages, and ranges. Urine volume, uterus weight, eosinophil, and basophil counts, and triglyceride, total bilirubin, and gammaglutamyl transpeptidase levels showed standard deviations of 30% or more. These historical control data would help to interpret the effects of test substances in routine toxicity and efficacy studies.