http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Rayamajhi, Nabin,Jung, Byeong Yeal,Cha, Seung Bin,Shin, Min Kyung,Kim, Aeran,Kang, Min Su,Lee, Kang Mu,Yoo, Han Sang American Society for Microbiology 2010 Applied and environmental microbiology Vol.76 No.14
<B>ABSTRACT</B><P>Fifteen nonrepetitive ampicillin-resistant <I>Salmonella</I> spp. were identified among 91 <I>Salmonella</I> sp. isolates during nationwide surveillance of <I>Salmonella</I> in waste from 131 chicken farms during 2006 and 2007. Additional phenotyping and genetic characterization of these 15 isolates by using indicator cephalosporins demonstrated that resistance to ampicillin and reduced susceptibility to cefoxitin in three isolates was caused by TEM-1 and DHA-1 β-lactamases. Plasmid profiling and Southern blot analysis of these three DHA-1-positive <I>Salmonella</I> serovar Indiana isolates and previously reported unrelated clinical isolates of DHA-1-positive <I>Salmonella</I> serovar Montevideo, <I>Klebsiella pneumoniae</I>, and <I>Escherichia coli</I> from humans and swine indicated the involvement of the large-size plasmid. Restriction enzyme digestion of the plasmids from the transconjugants showed variable restriction patterns except for the two <I>S</I><I>almonella</I> serovar Indiana isolates identified in this study. To the best of our knowledge, this is the first report of the presence of the DHA-1 gene among <I>Salmonella</I> spp. of animal origin.</P>
Rayamajhi, Nabin,Cha, Seung Bin,Kang, Mi Lan,Lee, Su In,Lee, Hee Soo,Yoo, Han Sang American Society for Microbiology 2009 Applied and environmental microbiology Vol.75 No.17
<B>ABSTRACT</B><P>Florfenicol resistance was analyzed in 230 enteric pig isolates collected between 1998 and 2006. PCR, plasmid profiling, Southern blot hybridization, and a mixed-broth conjugation assay suggested the intra- and interspecies plasmid-mediated transfer of florfenicol resistance among the isolates that exhibited MICs for florfenicol between 4 to 128 mg/liter.</P>
Quercetin Induces Mitochondrial Biogenesis through Activation of HO-1 in HepG2 Cells
Rayamajhi, Nabin,Kim, Seul-Ki,Go, Hiroe,Joe, Yeonsoo,Callaway, Zak,Kang, Jae-Gu,Ryter, Stefan W.,Chung, Hun Taeg Hindawi Publishing Corporation 2013 Oxidative medicine and cellular longevity Vol.2013 No.-
<P>The regeneration of mitochondria by regulated biogenesis plays an important homeostatic role in cells and tissues and furthermore may provide an adaptive mechanism in certain diseases such as sepsis. The heme oxygenase (HO-1)/carbon monoxide (CO) system is an inducible cytoprotective mechanism in mammalian cells. Natural antioxidants can provide therapeutic benefit, in part, by inducing the HO-1/CO system. This study focused on the mechanism by which the natural antioxidant quercetin can induce mitochondrial biogenesis in HepG2 cells. We found that quercetin treatment induced expression of mitochondrial biogenesis activators (PGC-1<I><I>α</I></I>, NRF-1, TFAM), mitochondrial DNA (mtDNA), and proteins (COX IV) in HepG2 cells. The HO inhibitor SnPP and the CO scavenger hemoglobin reversed the effects of quercetin on mitochondrial biogenesis in HepG2 cells. The stimulatory effects of quercetin on mitochondrial biogenesis could be recapitulated <I>in vivo</I> in liver tissue and antagonized by SnPP. Finally, quercetin conferred an anti-inflammatory effect in the liver of mice treated with LPS and prevented impairment of mitochondrial biogenesis by LPS <I>in vivo</I>. These salutary effects of quercetin <I>in vivo</I> were also antagonized by SnPP. Thus, our results suggest that quercetin enhances mitochondrial biogenesis mainly via the HO-1/CO system <I>in vitro</I> and <I>in vivo</I>. The beneficial effects of quercetin may provide a therapeutic basis in inflammatory diseases and sepsis.</P>
Vijay Rayamajhi,Dipesh Dhakal,송재경 한국생물공학회 2020 Biotechnology and Bioprocess Engineering Vol.25 No.3
Nodusmicin and 18-O-acetyl-nodusmicin were isolated as a co-metabolite of nargenicin from Nocardia sp. CS682. NgnL belonging to the α/β-hydrolase superfamily was heterologously expressed in Escherichia coli BL21 and was evaluated for its potential for acetylation of nodusmicin by in-vitro reactions. The generation of 18-Oacetyl nodusmicin was confirmed by high resolutionquadruple time of flight mass spectrometry (HR-QTOF-MS). Thus, it was concluded that NgnL can perform regiospecific acetylation of nodusmicin at 18-OH in presence of acetyl- CoA as an acetate donor. The enzyme kinetic parameters as Km and Vmax of the enzymes were also evaluated. Further, the biosynthetic role of the enzyme for acetylation of nodusmicin was confirmed by gene inactivation and complementation studies. Hence, from this study, it was established that NgnL is regiospecific acetyltransferase involved in the biosynthesis of 18-O-acetyl nodusmicin in Nocardia sp. CS682.
Tracking Eye Movement for Visual Cursor
Shashidhar Ram Joshi,Laxmi Rayamajhi Rawal 한국멀티미디어학회 2010 한국멀티미디어학회 국제학술대회 Vol.2010 No.-
To design a real-time, robust eye tracker system with human eye movement indication property using the movements of eye pupil an algorithm is designed. Eye tracker algorithm is implemented using the Continuously Adaptive Mean-Shift (CAMSHIFT) algorithm and the EigenFace method. Input image captured by the web cam is detected using the CAMSHIFT algorithm. Face area is passed through a number of steps such as color space conversion and thresholding. After these steps, areas for left and right eyes are determined using the geometrical properties of the human face. Search regions for left and right eyes are individually passed to the eye detection algorithm to determine the exact locations of each eye.