韓國人 乳癌組織의 Estrogen 受容體의 檢索

金政徹,李洙定,鄭泰浩,朴朗韻,黃一愚 慶北大學校 醫科大學 1992 慶北醫大誌 Vol.33 No.3

한국여성의 유방암에서 estrogen수용체 및 progesterone수용체의 분포를 알아보기 위하여 39례의 유방암조직에서 이를 측정하였다. Estrogen수용체는 33.3%에서 양성으로 5.42에서 230.02f㏖/㎎ protein까지의 값을 나타내었고 progesterone수용체는 5.34내지 66.60f㏖/㎎ protein의 함량을 가진 56.4%에서 양성이었다. 폐경기 이전은 폐경기 이후에 비해 estrogen수용체와 progesterone수용체가 모두 양성인 경우가 더 많았고 모두 음성인 경우는 더 적었으며 환자의 연령이 50세 이하인 경우의 progesterone수용체 양성율은 50세 이상의 경우보다 두배 이상 높았다. 또한 액와임파절전이가 전혀없는 경우가 1개이상 전이된 경우보다 훨씬 높은 estrogen수용체 양성율을 보였으며 TNM병기 Ⅱ는 병기Ⅱ나 Ⅳ보다 estrogen수용체 및 progesterone수용체 양성율이 더 높은 것으로 나타났다. 한편 조직학적 유형과 호르몬 수용체와의 관계는 대부분이 infiltrating ductal carcinoma인 까닭에 의미 있는 결과를 얻지 못하였다. 이상의 결과에서 나타난 estrogen수용체 및 progesterone수용체와 유방암의 여러 임상적 병리조직학적 측면들과의 상관관계로 미루어 볼 때 임상검사실에서의 통상적인 호르몬수용체 측정법 확립은 유방암의 치료 및 예후판단에 매우 유용하리라 기대된다. The levels of estrogen receptors (ER) ad Progesterone receptors (PR) in 39 breast cancer tissues have been determined and a correlation of both receptor levels with the histopathologic findings and clinical features was attempted. The ER levels ranged from 0 to 230.02 fmol/㎎ cytosol protein and the PR levels ranged from 0 to 66.60 fmol/㎎ cytosol protein. 33% of the specimens were ER+ and 56% were PR+. The group younger than 50 years had a higher percentage of positive PR status. The positive rate of ER showed no significant difference between pre and postmenopause, while those of PR was decreased from 67% in pre-to 41% in postmenopause. 82% of the histologic type were infiltrating ductal carcinoma and 74% of patients were stage Ⅱ of TNM classification. These results indicated that there was strong correlation between age, menopausal status, axillary lymph node metastasis, TNM stage and receptor status.

未成熟 흰쥐子宮의 Estrogen 受容體의 細胞內 分布 및 Progesterone 受容體에 對한 Tamoxifen의 作用

朴朗韻,鄭泰浩,曺準承 慶北大學校 醫科大學 1990 慶北醫大誌 Vol.31 No.3

Estrogen의 작용은 세포내의 특이수용체와의 결합을 통해 특정유전자의 발현을 유도함으로써 이뤄진다는 정도로 밝혀져 있을 뿐 그 자세한 기전에 대해서는 잘 이해가 되지 않고 있어 estrogen 및 항estrogen의 표적조직내 존재여하에 따른 estrogen수용체의 세포내 분포에 대해서 알아보고자 하였다. 미성숙 흰쥐에 100㎍의 tamoxifen을 일회 투여하고 2, 6, 12, 24, 48, 72시간이 지난 후에 자궁에서 나타나는 변화를 살폈다. 자궁무게와 자궁내 DNA함량, 그리고 progesterone수용체의 함량은 tamoxifen투여후 시간이 지남에 따라 모두 증가되었다. Tamoxifen을 투여하지 않은 대조군에서의 estrogen수용체 핵과 세포질에 각각 50%씩 분포하였고 tamoxifen투여후에는 세포질내수용체가 점차 감소하고 핵내수용체는 증가되는 경향을 보였다. 또한 대조군에서는 거의 모든 수용체가 유리형으로 존재하였던데 반해 tamoxifen의 투여로 결합형 수용체의 증가와 함께 유리형의 감소가 초래되었다. 이상의 결과로써 미성숙 흰쥐 자궁세포내에는 핵과 세포질 모두에 유리형 수용체가 존재하며, tamoxifen은 이들 양 분획의 수용체에 모두 결합하고 세포질내에서 핵내로 수용체의 전이를 유발하며 동시에 새로운 수용체의 합성도 유도하는 것으로 사료된다. Although it is widely accepted that specific intracellular receptor proteins are involved in the estrogenic regulation of gene expression and growth in reproductive tissues, the precise nature of the regulation is poorly understood. Among the unresolved issues are the distribution and dynamics of the estrogen receptor protein in target tissues in the presence and absence of estrogens and antiestrogens. In this study, the effect of antiestrogen, tamoxifen, on subcellular distribution of estrogen receptor in immature rat uterus was determined. A single dose of tamoxifen(100㎍/rat) increased uterine wet weight, DNA content, and cytoplasmic progesterone receptor content 2, 6, 12, 24, 48, 72 hours after injection. Total estrogen receptor content progressively increased, peaked 48hr post-injection, while cytoplasmic receptor content decreased. In control uteri, nuclear estrogen receptors were almost unoccupied, but occupied receptors increased afrter tamoxifen treatment. The amount of unoccupied cytoplasmic receptors rapidly decreased 2hr postinjection and then gradually restored to that of control which was 74% of cytoplasmic receptors. These results suggest that in immature rat uterus both cytoplasm and nucleus contain unoccupied, free estrogen receptors and also suggest that tamoxifen can bind both cytoplasmic and nuclear 'estrogen receptros, increase total estrogen receptor content, and elicit some estrogenic effect.

木材의 맞춤 技法에 關한 硏究

申朗浩,金政虎 三陟大學校 1998 論文集 Vol.31 No.3

목재의 접합이란 목재가 지니고 있는 단점을 보완하는 방법 중의 하나로서 각각의 기능이나 용도에 맞게 두 개 이상의 부재를 이어서 한 개의 부재로 접합하는 것을 이음이라 하고, 각각의 부재를 쓰임에 따라 직각 또는 경사지게 맞추는 방법을 맞춤(짜임)이라 한다. 가구나 건축에서의 맞춤은 그 정밀도에 따라서 제품의 수명도 달라지고, 같은 조건에서도 어떤 맞춤 방법을 사용하느냐에 따라 제품의 아름다움이나 질이 달라지므로 맞춤의 기법을 공정과 함께 세부적으로 인지하고 있어야 한다. 목재의 접합은 일정한 강도를 유지하는 것 외에도 힘에 대한 변위(變位)가 적어야 하며, 적절한 강성을 지녀야 하는데 구조재에 작용하는 압축, 인장, 전단 등의 응력을 파악하고 맞춤 하고자 하는 장소와 부재의 구조 방법에 따라 적절한 기법을 선택하여 사용하여야 한다. 이러한 의미에서 본 장에서는 맞춤 기법의 종류와 사용 방법 공정 등에 대하여 연구하고, 나아가 맞춤에 사용되는 공구류에 대하여도 세부적으로 살펴본다. The technique of joining is one of the most effective methods for the correction of shortcoming of woods. According to the function and the uses of it, it has two different main types. One called 'connection' is a method which is used when we want pur at least two boards together side by side, and another called 'joint' is used when we put pieces of wood together at the end of pieces at a right angle or slant. An accurate joint can make all the difference in the life span of furniture and architecture. Even though all the conditions are the same in processes, the appearances and the qualities of outcoming can be very different by the types of joint that is used. So, it is necessary to know about all the details of joining techniques along with the processes of wood working. Because joints are the places where the structures of furniture or architecture should be properly selected according to the factors like the compression stress, the expansive stress, and the conductivity of each constructions. In these senses, in this chapte, we'll study more about all the different types and the processes of joints and we'll get more specified information about tools for joining techniques as well.

木材의 材料分析에 關한 硏究

申朗浩,金政虎 三陟大學校 1993 論文集 Vol.26 No.-

나날이 발전해가는 경제성장과 더블어 한층 심각해져만 가는 환경오염을 피부로 느끼면서 생활하는 현대인들은 누구나가 자연 그 지체로 돌아가고 싶다는 마을을 가지리라 생각한다. 근본적인 자연 그 자체로 들어가지 못하는 마음을 현대 생활속에서 항상 접하는 어떠한 물건에서 찾는다면, 그것은 바로 인간에게 가장 자연스러운 친금감을 주는 재료인 나무로 만들어진 제품이라 하겠다. 즉, 목재는 역사가 시작된 이래 부드럽고 따뜻하며 자연스런 친금감을 준다는 질감의 특성으로 인하여 인간과 더블어 가장 밀접한 관계를 계속 유지해 왔다고 볼 수 있다. 따라서 예부터 우리나라에서는 삼면이 바다로 둘로 싸여 있고, 남북으로 길게 위치해 있다는 지리적, 위치의 특성으로 인하여 수종이 매우 다양하고 풍부하였다. 그래서 이러한 목재는 가구재나 건축재로 많이 사용되어져 왔다. 그러나 새로운 건축자재가 다양하게 발달됨으로써 목재의 사용 용도 또한 건축용재에서 장식용재 및 공예재료로 차츰 전환되어 가고 있다. 이러한 의미에서 우리가 쉽게 접할 수 있는 목재의 종류와 구조 및 성질에 관하여 연구하고, 더믈어 목재를 기본 재료로하여 만들어진 새로운 재료의 특성 및 종류에 관하여 살펴본다. Almost every modern people who is living a life getting the feel of environmental polution wit the skin that is worsening in line with the increasing economic growth, is believed to have a great mind to return to nature. If we can suggest an object which is touched in everyday life the moderns with who can not return to the orginal nature, it will be works made of wood which gives a feeling of intimacy to human beings. That is, wood is regarded to have had a close relationship with man due to its characteristic that have produced friendly feelings to man since the begining of human history. Accordingly, owing to the geographic character of our country that is surrounded by sea on all sides but one and stretches from south to north, it abound with and has a variety kind of wood. So such woods have been used as materials for building and furniture. But in accordance with various development of new building materials, the use of wood have undergone a change from the materials for building to the ones for decoration and craftwork. In this respect, the kinds, structure and character of wood which is easily touched with us, is studied and the special character and kinds of new materials that are made on the basis of wood, are examined.

華角工藝를 利用한 工藝品 開發에 關한 硏究

申朗浩 三陟大學校 1993 論文集 Vol.26 No.-

Among traditional craft arts in Korea, Wha-Gak handicraft is recognized as an orgianl craft art, because its material has been improved and developed. The author attempted to find out its historic and social background and production processes through boks and trial manufacture. The author tried to recognize its value as a traditional craft arts. As for Wha-Gak handicraft , it is intially made by sheets of thin horn paper. On this paper, various patterns are drawn with colors and attached on the object as a decoration. It was influenced by ancient Chinese Dae-Mo-Bok-Chae technique and it is estimated that these days horn paper is used instead of Dae-Mo as a main material. The oldest remain that we know are produced during the Silla Dynastry and kept at Jung Chang Won in Japna, The essence of color on Wha-Gak handicraft is pattern design and the horn paper. Judhing from patterns and colors on the remains we can understand that popular belief and story have been shaped and symbolized in limited areas. Chinese color, gold and silver foil have been used and flowers, longevity symbols, dragons, tigers, magpie tigers and jumping carps seen oncfolk painting and embroidery have been repeatedly described on the remains. These old remains are feminine articles such as sewing boxes, mirrors, Korean socks case, combs, fans, jewelry boxes and sewing measures. The reason why this Wha-Gak handicraft has been developed so slow is that its production process was difficult, it was restricted because it was regarded as aristocratic craft arts and remains could not be spread out because of horn paper's weakness. I think that recently production problems are solved by recognition of traditional craft arts, mechanized production of horn paper and development of tools.

螺鈿漆을 利用한 工藝品 開發에 關한 硏究

申朗浩 三陟大學校 1998 論文集 Vol.31 No.3

The development of craftworks that is based on the beauty of tradition with the life and spirit of a nation and that takes the modern life style into cosideration not only paves the way for the restoration of the vein of a vanishing tradition of craft but also gives an opportunity to success traditional culture. However, the abandonment of principles and the thoughtless inflow of western culture in modern Korean history led the Korean traditional craft to a state of structural disorder, Besides, the lack of general understanding of tradition of Korean craft became a barrier to the development of traditional handcraft and even threatened the maintenance of its life. Today, the world is making efforts to find an active and diversified way of improvement of products by the intensification of the international competitive power through technical innovations, introductions of new materials and new designs. according to this tendency, we persue a renovation of living environment by the development of diverse materials and techniques with a renewed understanding of the traditional craft, but we still have a lot of problems in development of characteristic which is peculiar to the Korean traditional craftwork. In this point of view, it will be very significant outcome as a basis of succession of tradition and creation of new design if we can apply the traditional way of Korean Nacre Inlay lacquer Handcraft to the contemporary living environment and to the preference of people. In addition, we will be able to show a possibility to innovate products as an cultural things as well as the development of crafts to export for the national economy. Therefore, in this project. I pur an emphasis on the development of Korean Nacre Inlay Lacquer Handcraft through design based on the tradition, understanding of the origin, development, way of proceeding, material and techniques of it.

골프장연습장 이용 고객의 전환장벽 요인에 따른 시장세분화

김애랑(Ae Rang Kim),전호문(Ho Moon Jun),조우정(Woo Jeong Cho) 한국사회체육학회 2013 한국사회체육학회지 Vol.0 No.53

The purpose of this study was to segment the customers of driving range by clustering them based on switching barriers. The second purpose was to identify the characteristics of each cluster. The third purpose was to examine the differences of satisfaction and switching intentions of each cluster. In order to measure the switching barriers, 13 items divided into 3 factors, which were 7 items for switching cost (Cronbach`s α = .875), 3 items for interpersonal relationship (Cronbach`s α = .686), and 3 items for attraction of alternatives (Cronbach`s α = .764) were used. A total of 431 responses were analyzed with the statistical methods such as frequency analysis, exploratory factor analysis, K-mean cluster analysis, Chi-square analysis, One-way ANOVA, and multiple regression analysis. The findings for this study were as follows. First, the three clusters were identified based on switching barriers. Second, each cluster was explained by the personal characteristics of the customers of driving range. Third, statistically significant differences in switching intentions and satisfactions were found among clusters.

Notes : Isolation and Characterization of Flavobacterium johnsoniae from Farmed Rainbow Trout Oncorhynchus mykiss

( Rung Karn Suebsing ),( Jeong Ho Kim ) 한국수산과학회(구 한국수산학회) 2012 Fisheries and Aquatic Sciences Vol.15 No.1

Flavobacterium johnsoniae was isolated from farmed rainbow trout Oncorhynchus mykiss in Korea, and its biochemical and molecular characterization was determined. Yellow-pigmented bacterial colonies were isolated from 18 of 64 fish samples (28.1%) on trypticase soy agar plates, and their biochemical profiles were characterized by API 20E and API 20NE test kits. F. johnsoniae was identified by biochemical phenotyping of factors including rapid gliding motility, Gram-negative condition, oxidase- and catalase-positive status, Congo red absorption, nitrate reduction, β-galactosidase production, acid production from glucose, and gelatin and casein hydrolysis. PCR and subsequent sequencing of 16S rRNA confirmed that the yellow-pigmented colonies were most similar to F. johnsoniae. The alignment analysis of 16S rRNA sequences also showed that all 18 rainbow trout isolates had highly similar homologies (97-99% identity). One isolate was selected and named FjRt09. This isolate showed 98% homology with previously reported F. johnsoniae isolates, and in phylogenetic analysis was more closely grouped with F. johnsoniae than with F. psychrophilum, F. columnare, or F. branchiophilum. This is the first report on the occurrence and biochemical characterization of F. johnsoniae isolated from rainbow trout in Korea.

Esteblishment of secretome analysis method from human bone marrow-mesenchymal stem cell differentiated-osteoblasts

Ho KIM,Seul-Gi OH,Hae Reon SON,Sa Rang KIM,Young Ha RYU,Yong Guk KIM,Jang Hyeon EOM,Dabin SEONG,Min-Jung KIM,Jounghee BAEK,Joon Ho EOM,Ki Dae PARK,Chiyoung AHN 한국생물공학회 2018 한국생물공학회 학술대회 Vol.2018 No.4

P151 : A clinical and epidemiologic study of actinic keratosis over the last ten-year period (2003-2013) in Gwangju-Chonnam area

( Ki Rang Moon ),( Sook Jung Yun ),( Seong Jin Kim ),( Seung Chul Lee ),( Young Ho Won ),( Jee Bum Lee ) 대한피부과학회 2014 대한피부과학회 학술발표대회집 Vol.66 No.2

Background: Actinic keratosis(AK), also known as solar keratosis, are common lesions on the sun-exposed skin of elderly patients. The most important risk factor is thecumulative sun exposure. As an aging population rises, prevalence of AK increases with advancing age. Objectives: To analyze the clinical and epidemiological features of actinic keratosis(AK) in outpatient of Chonnam National University Hospital(CNUH). Methods: We reviewed all of the AK cases diagnosed of clinical and pathological examination in the Department of Dermatology at the CNUH from 2003 to 2013 retrospectively. Results: Total 288 patients of the AK diagnosed from 2003 to 2013. Of these, 94(32.6%) and 194(67.3%) were male and female patients, respectively. Patients’ average age was 76.5 years. The mean duration of disease was 29.3 months. Most patients (180 cases, 40.0%) had a single lesion, and there were 55 patients with more than 3 lesions. Of the total (450 cases), 91.6% (412 cases) of the lesions were located on the face, especially on cheeks (56.9%, 256 cases) and on nose (10.2%, 46 cases), in order. The patients were treated by cryotherapy(269, 59.7%), followed by excision(129, 28.7%) and CO2 laser (30, 6.7%), topical agents with imiquimod 5%(22, 4.9%) and photodynamic therapy using methyl aminolevulinate (8, 1.8%). Of 288 patients, 17 patients (5.9%) were accompanied by the skin cancers. Conclusion: The survey leads us to identify the epidemiologic and clinical feature of AK in Gwangju-Chonnam area.