The effects of antibiotics on the reproductive physiology targeting ovaries in the Asian tiger mosquito, Aedes albopictus

Ha Young‐Ran,Jeong Se‐Jin,Jang Chang‐Won,Chang Kyu‐Sik,Kim Hyun‐Woo,Cho Shin‐Hyoung,Lee Hee‐Il 한국곤충학회 2021 Entomological Research Vol.51 No.2

Mosquitoes have adapted to various environmental conditions. Symbionts with mosquitoes impact this adaptation in different environments. In the field, mosquitoes could get exposed to antibiotics during their developmental period, which could reduce or eliminate their symbiotic microbes. However, the side effects of the antibiotics on the ovary and reproductive physiology of the Asian tiger mosquito, Aedes albopictus remains unknown. In this study, we investigated the effects of tetracycline and combinations of rifampicin and tetracycline at environmentally acceptable levels on the reproductive physiology of ovaries in Ae. albopictus. Rifampicin and tetracycline in combination reduced the hatching rate and fertility of Ae. albopictus compared to the untreated control group. These antibiotics induced histopathological damage and reactive oxygen species production in the ovaries. The combination of antibiotics decreased the expression of surface protein of Wolbachia (WSP) in Ae. albopictus. Additionally, the expression of Toll like receptor 2 (TLR2) and Myd88 were triggered by the combinations. The findings demonstrate the detrimental effects of antibiotics, particularly combinations of rifampicin and tetracycline, on the reproductive capacity of Ae. albopictus females.

신라검무 및 동일강도의 트레드밀 운동시 심박수, RPE 및 혈중 젖산농도 변화의 비교

오향란,안나영,김기진 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.6

This study was performed the comparison of physiological function between a sword dance and treadmill running, For the positive factors of sword dance through physical activity and music component on the psycho-hysiological change. Subjects were consisted of 20-aged 16 female sword dancer of 2 groups, as skillful dancer group(n=B) and novice group(n=B), The results were as follow: In graded maximal testing, VO₂max, anaerobic threshold, and recovery capacity after maximal exercise in skillful group showed a higher than novice group. Change of heart rate during a sword dancing is almost similar level to optimal intensity in aerobic exercise program, so sword dance could be suggested an effective exercise program Physiological stimulus to cardiopulmonary function is different response as the different career of sword dance, because novice group showed a significant(p<.05) higher heart rate in sword dance than treadmill running. Blood lactate concentration showed a similar response between sword dancing and treadmill running in both 2 groups. Novice group showed a higher RPE in sword dancing than treadmill running, but skillful group showed a lower RPE in sword dancing than treadmill running. These results mean that the acquainted level of sword dance affected to the psychological response during sword dancing In these result, hypothesis of the similar blood lactate change between sword dancing and treadmill running could be accepted, but RPE is different response as the different career of sword dance.

Effect of TiO2@Carbon Nanotubes and Praseodymium on the Microhardness and Corrosion Properties of AZ91 Alloy

Qianwen Ran,Hong Yan,Jiajia He,Ning Li,Yushun Lei 대한금속·재료학회 2022 METALS AND MATERIALS International Vol.28 No.8

Effect of 1 wt% TiO2@carbon nanotubes (TiO2@CNTs) and 1 wt% praseodymium (Pr) on the microhardness and corrosionproperties of AZ91 magnesium alloy were discussed. The microhardness test, electrochemistry test that AZ91 magnesiumalloy with 1 wt% TiO2@CNTs and 1 wt% Pr addition exhibits excellent microhardness (111 HV), low corrosion currentdensity (0.165 mA·cm−2), and low polarization resistance (537.15 Ω·cm2) compared with AZ91 magnesium alloy (89 HV,0.184 mA·cm−2, 261.06 Ω·cm2). After 1 wt% TiO2@CNTs and 1 wt% Pr added to AZ91, the average crystallite size wasreduced and microhardness was increased. Additionally, the Al11Pr3phase formed by the combination of Pr and Al transformedthe morphology of β-Mg17Al12 phase into weeny particles and decreased the volume fraction of β-Mg17Al12 phase. The reduction and refinement of cathode phase (β-Mg17Al12 phase) weakened the micro-galvanic corrosion and sloweddown the corrosion rate.

Determination of Acyl-lysophosphatidic Acid in Plasma by Tandem Mass Spectrometry

Yoon, Hye-Ran 덕성여자대학교 약학연구소 2005 藥學論文誌 Vol.16 No.1

Analysis of acyl lysophosphatidic acids(LPAs) has clinical importance as a potential biomarker for ovarian and other gynecological cancers or obesity in the aspect of prevention. Here we report simple sample preparation and analytical method developed with high sensitivity and specificity for the early detection of gynecological cancers to improve the overall outcome of this disease. We established a novel quantification method of acyl-LPAs in plasma by electrospray negative ionization tandem mass spectrometry (MS-MS) using multiple reaction monitoring mode without conventional TLC step. Protein-bound lipids, acyl-LPAs in plasma were extracted with methanol : chloroform (2:1) containing LPA C14:0 as internal standard under acidic condition. Following back extraction with chloroform and water, centrifuged lower phase was evaporated and reconstituted in methanol and then analyzed. Using ESI-MS-MS with negative ionization MRM mode, all the species of LPAs were completely separated from plasma matrix without severe interferences. For MRM mode, Q1 ions selected were m/z 409, 433, 435, 437 and 457 which corresponds to molecular mass [M-H]- of C16:0, C18:2, C18:1, C18:0 and C20:3 LPA, respectively. Q2 ions selected for MRM was m/z 79, phosphoryl product. Using MS/MS with MRM mode, all the species of LPAa were completely separated from plasma matrix without severe interferences. This method allowed simultaneous detection and quantification of different species of LPAs in a plasma over a linear dynamic range of 0.01 - 25 (mol/L. The method detection limit was 0.3 pmol/ml with correlation coefficient of 0.9983 in most LPAs analyzed. When applied to the plasmas with normal and gynecological cancer patient, this new method differentiated two different groups by way of total LPA level.

Protective Effect of Curcumin and Aqueous Extract of Onchengyeum on CCl_(4)-induced Hepatotoxicity

Seung, Keum Ran,Jung, Ki Hwa 덕성여자대학교 대학원 2005 덕성여자대학교 대학원 논문집 Vol.7 No.-

An aqueous extract of oriental herbal composition named Onchengyeum and curcumin, an antioxidant isolated from turmeric (Curcuma longa Linné) reduced hepatotoxicity induced by carbon tetrachloride (CCl_(4)). Improved liver function was observed by measuring the activities of aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), blood urea nitrogen (BUN), creatinine (CRE), total cholesterol (TCHO), triglyceride (TG), low density lipoprotein cholesterol (LDL-CHO), high density lipoprotein cholesterol (HDL-CHO), total protein (TP), albumin (ALB) and total bilirubin (BIL) in serum. Hepatic parameters monitored were levels of cholesterol (CHO), triglyceride (TG), malondialdehyde (MDA) and content of cytochrome P450 (CYP), level of glutathione (GSH), activities of NADPH-CYP reductase, superoxide dismutase (SOD), catalase (CAT), glutathione S-transferase (GST), glutathione reductase (GR), glutathione peroxidase (GPx). The histopathological examination showed that the treatment of Onchengyeum and curcumin relieved the ballooning degeneration of hepatocytes which had been generated by CCl_(4). The results suggested that hepatoprotective effects of Onchengyeum and curcumin possibly are due to their promising antioxidative activity.

改革의 傳播와 改革者의 特徵

金蘭洙 연세대학교 교육대학원 1972 교육논집 Vol.5 No.1

마늘의 Allicin이 사람 단핵세포의 사이토카인 생산 유전자의 발현에 미치는 영향

박란숙 한국식품영양학회 2002 韓國食品營養學會誌 Vol.15 No.3

마늘의 주요 성분인 allicin 투여 후 유도되는 사람 말초혈액의 단핵구의 유전자 발현에 미치는 allicin 의 효과를 규명하였다. DNA microarray를 이용하여, allicin이 chemokines, cytokine 면역관련 유전자 및 신호전달 관련 유전자의발현을 유도하는 것을 확인하였다. 반대로 allicin은 Thl type의 획득면역 관련 유전자의 발현을 억제하였다. 염증세포에 있어서 allicin은 억제효과 및 자극 효과를 동시에 보여주었다. 이는 allicin이 휴지기 세포에서 먼저 증가시킨 특정 유전자의 발현을 이후에 감소시키는 결과를 보여주는 것으로 positive와 negative 효과를 발휘하는 새로운 기전을 제시하는 것이다. Allicin에 대한 광범위하고 새로운 관심을 고려해 볼 때 본 연구에서 보여주는 많은 유전자의 발현 양상은 좀 더 특정적이고 효과적인 치료법을 고안하는 데 유용할 것이다. The effect of allicin, the major component of garlic (Allium sativum), on the gene expression profiles of peripheral blood mononuclear cells from healthy donors was analyzed. DNA microarray which can detect expression signal of 862 genes revealed that allicin induced the expression of cytokine, chemokine, and immune-related genes in peripheral blood mononuclear cells. In contrast, allicin repressed the expression of adaptive immune-related genes, which are expressed in T helper I lymphocytes. Simultaneous inhibitory and stimulatory effects of allicin were found on inflammatory cells. It is likely that allicin down-regulated the expression of specific genes that were previously up-regulated in resting cells, suggesting a new mechanism by which they exert positive and negative effect. Considering the broad and renewed interest in allicin, the profiles we describe here will be useful in designing more specific and efficient treatment strategies.

巴金의 《추운 밤》의 현실주의 연구

朴蘭英 中國語文硏究會 1992 中國語文論叢 Vol.5 No.-

巴金의 "《휴식의 뜰 (憩園)》연구

朴蘭英 中國語文硏究會 1995 中國語文論叢 Vol.9 No.-

소비자 안티사이트에 대한 연구 : 평가모형개발, 평가 및 발전방안의 모색

김난도,김선욱 한국정보사회진흥원 2003 정보화정책 Vol.10 No.4

본 논문은 최근 주목을 받고 있는 인터넷을 통한 소비자 운동 중에서 가장 관심을 끄는 소비자 안티사이트를 개념 규정하고, 그 현황을 살펴 본 후, 바람직한 안티사이트 구축을 위한 평가모형을 개발하여, 그에 따라 현재 우리 나라의 대표적인 안티사이트들을 평가해 보고자 한다. 안티사이트의 적절한 평가모형을 개발하기 위하여, 국내외의 사이트 평가기준을 종합하고 안티사이트의 특성을 고려하여 컨텐츠, 연계성, 인터페이스 및 관리의 3영역으로 나누어 평가지표를 개발하였다. 또한 안티사이트의 컨텐츠 구성과 관련하여 안티사이트 이용자를 불만표출을 위한 이용자, 정보수집 소비자, 자사 안티 정보수집을 위한 기업측 종사자의 니즈를 구별하여, 소비자 안티사이트가 감정적인 불만표출의 장이 아니라 국가경제에 기여할 수 있는 실질적인 사이트로 발전시킬 수 있는 방안을 모색하였다. 마지막으로 소비자 안티사이트를 건전한 방향으로 정착시키기 위한 여러가지 정책적 방안을 함께 모색하였다. This paper delves into the consumer anti-sites, which show new horizon for internet consumerism. The study first studies the concept and status quo of consumer anti-sites and then evaluate current anti-sites by developing model and indices of site evaluation. In developing the model, the paper make up three aspects of site evaluation: contents; linkage; and interface and maintenance. The paper considers not only the users who wish to compalin about their discontent but also the users who simply want to gather information for future purchase and suppliers who intend to gather information about their management in evaluating contents. Lastly, the paper also suggests policy implications to make better consumer anti-site culture.