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      • Temperature and relative humidity mediated life processes of Spodoptera species (Lepidoptera: Noctuidae)

        Rameswor Maharjan,Seoyeon Hong,Youngnam Yoon,Yunwoo Jang 한국응용곤충학회 2023 한국응용곤충학회 학술대회논문집 Vol.2023 No.10

        Spodoptera species (S. exigua and S. litura) are important pests of several crops and vegetables in Korea. We investigated development processes of Spodoptera species under constant temperatures (20, 25 and 30 oC) regimes and relative humidity (RH) (30-35, 50-55, 70-75, and 90-95%) conditions. We collected eggs of Spodoptera species by releasing them into a rectangular box inner walls covered with a sheet of white paper. Temperature and RH significantly impacted on oviposition, immature survival, adult emergence and longevity of Spodoptera species. Maximum number of eggs, shorter developmental time, higher adult emergence with longer longevity were reported in 70-75% at 30 oC. Minimal eggs and larval survival were recorded in 30-35% and 90-95% RH, respectively. This results suggest that temperature and RH had individual apparent effect on the developmental processes of Spodoptera species instead interactive effect. Therefore, there is chance to cause a significant damage to field crops and vegetables in 70-75% at 30 oC.

      • KCI등재

        Oviposition preference and development of Maruca vitrata (Fabricius) (Lepidoptera: Crambidae) on different radiofrequency fields

        Rameswor MAHARJANM,배순도,김길하,윤영남,장윤우,김용철,이휘종 한국곤충학회 2019 Entomological Research Vol.49 No.5

        Maruca vitrata is considered as one of the major impediments in boosting up the production of tropical legumes. Since chemical pesticides have an adverse effect on human health and environment, there is an urgent need for developing a nonchemical based method to replace chemical insecticides for controlling of pests. We investigated the oviposition, development and adult performances of M. vitrata at five radiofrequency levels of 0 (control without frequency), 5, 10, 20, and 30 kHz on artificial diet. In addition, the effects were also studied in three successive generations (Parent, F1- and F2- generations) of M. vitrata. The results showed that radiofrequency application had significant effects on the life-variables of M. vitrata. Radiofrequency exposures on M. vitrata not only impacted on oviposition, developmental time, adult longevity, and adult weight but negative effects were also transmitted to the successive generations. Oviposition was found to reduce as generation passed after exposed to radiofrequencies. Total developmental times from egg to adult emergence were longer with shorter longevity in case of radiofrequency treatment than untreated. Decreasing the radiofrequency level further increased time for development of adults as generation passed. The shortest adult longevity occurred at 5 kHz treatment. The outcomes of this study are discussed in terms of targeting susceptibilities to radiofrequency as an alternative to chemical treatments.

      • Efficacy of insecticides on Liriomyza huidobrensis (Blanchard) (Diptera: Agromyzidae) under greenhouse and laboratory condition

        Rameswor Maharjan,Chuleui Jung 한국응용곤충학회 2013 한국응용곤충학회 학술대회논문집 Vol.2013 No.10

        Liriomyza species are the major pests of greenhouse and field crops. It was as minor pests in past, but the pest population increased rapidly such as L. trifolii, L. huidobresnsis and L. sativae during recent years is attributed to application of ineffective insecticides and developed insecticide resistance. This study was carried out to determine the efficacy of insecticides groups, Anthranilic diamide, Biopesticide, Carbamate, Neonicotinoid, Organophosphate, Pyrethroid and Oxadiazine, recommended for the control of leafminer species in Korea. Bioassay tests were done on first, third larval stages and adults under glasshouse and laboratory conditions respectively. The result showed significant differences in toxicity among the tested insecticides. The mortality of abamectin and lepimectin and spinosad were higher (>50%) in larvae stages. Etofenprox insecticide was not effective on larvae stage. The toxicity of Cartap hydrochlorid (Neonicotinoid) was higher (100%) in adult insects, followed by thiamethoxam, etofenprox and indoxacarb. Abamectin and lepimectin showed the decreasing toxicity level as insect stage developed. Abamectin showed least toxicity (26.67%) in adult stage. Spinetoram and emamectin benzoate showed higher toxicity (>50%) in all the insect stages tested. It could be suggested that abmectin and lepimectin have high efficiencies on control of larvae. However, spinosad and emamectin benzoate has higher potential on the control of both larvae and adult insects.

      • Effects of Temperatures on the Development of Liriomyza huidobrensis (Blanchard) (Diptera: Agromyzidae)

        Rameswor Maharjan,Chuleui Jung 한국응용곤충학회 2014 한국응용곤충학회 학술대회논문집 Vol.2014 No.10

        The development of Liriomyza huidobrensis (Blanchard), an economic pest of wide range of field crops and ornamental plants, was studied under laboratory conditions at six constant temperatures (10, 15, 20, 25, 27 and 30oC). The total development period decreased with increasing temperatures between 15 and 27oC. In 10oC, eggs did not hatch and in 30oC, pupa did not survive. So, it may be assumed that these temperatures fall outside the development. Linear regression was used to describe the relationship between development rate and temperature. For total immature development (egg to adult) required 250 degree-days (DD) above the threshold of 9.75oC. The developmental data were fitted to a nonlinear temperature-dependent model (Lactin-1) for best described of development. Development rates of males and females did not differ in 20 and 25oC. High immature mortality was recorded in 27oC. Sex ratio of the surviving individuals was 0.62 from this study. Thermal requirements and temperature thresholds can be used to forecast the occurrence, number of generations and population dynamics of L. huidobrensis.

      • KCI등재

        Mass production of Diglyphus isaea (Hymenoptera: Eulophidae), a biological control agent of a Korean population of potato leaf miner Liriomyza huidobrensis (Blanchard) (Diptera: Agromyzidae)

        Rameswor Maharjan,권민,김주일,정철의 한국곤충학회 2017 Entomological Research Vol.47 No.2

        The potato leaf miner, Liriomyza huidobrensis (Blanchard), is an important pest of potato throughout the world, including Korea. A method was developed for mass rearing the parasitoid Diglyphus isaea (Walker) using faba bean, Vicia faba L. (Fabaceae), as the host‐plant and L. huidobrensis as the insect host. Faba bean plants were planted in pots and maintained in a greenhouse for about 15 d. Pots were then exposed to adult leaf miners in oviposition cages for 4 h. Plants containing late second to early third instar larvae were exposed to adult D. isaea in parasitoid cages for 24 h. The leaf area per pot after 15 d was 597.9 cm2, which produced 103.33 larvae per pot. The number of adult parasitoids emerging per pot was 72.5; about 41% of these were female. The daily cost of parasitoid production was USD20.95 per 1000 individual parasitoids. The methodology developed for D. isaea could be used to rear other ectoparasitoids such as Hemiptarsenus spp. and Pnigalio sp. with different insect hosts like L. trifolii. This is why this mass‐rearing information is important for securing test insect materials for ecological and biological study of Liriomyza species, and also for developing a biological control for Liriomyza species other than L. huidobrensis by mass production of associated natural enemies. We are facing rapid agro‐ecosystem changes including pest systems. Continuous monitoring of Liriomyza in solanaceous crops is needed.

      • Morphological and genetic characteristics of Nysius species (Heteroptera: Lygacidae: Orsillinae) of Korea, with description of a newly reported species

        Rameswor Maharjan,Soondo Bae,Hwijong Yi,Wonhoon Lee,Yoonnam Young,Yunwoo Jang 한국응용곤충학회 2018 한국응용곤충학회 학술대회논문집 Vol.2018 No.10

        Nysius Dallas, 1852, is one of the most common and widely distributed genera under the superfamily Lygaeoidae. Species under this genus are hard to identify due to similarity of the species and variability of the coloration. The Nysius species were collected with the help of aspirator and plastic vile in the perilla crop fields in RDA, Miryang, Korea. Korean species of the Nysius were identified, and three species including a newly reported species N. inconspicuus were recognized. Morphological and genetic characteristics of species were illustrated, and a key to species of Korean Nysius was provided. The DNA barcoding information of N. plebeius and N. inconspicuus were recorded.

      • Olfactory Response and Feeding Preference of the Liriomyza huidobrensis (Blanchard) (Diptera: Agromyzidae) to Potato Varieties

        Rameswor Maharjan,Chuleui Jung 한국응용곤충학회 2013 한국응용곤충학회 학술대회논문집 Vol.2013 No.04

        The potato leafminer fly, Liriomyza huidobrensis (Blanchard) (Diptera: Agromyzidae) is a polyphagous pest attacking many vegetables and ornamental plants. We found L. huidobrensis were infesting potato crop in Miryang and Goreyong in 2011. Five potato (Solanum tuberosum) varieties (Ghowun, Daeri, Sumi, Chubeak, and Seohong) were tested for the preference of L. huidobrensis. In the olfactormeter bioassay, leafminers chose the Ghowun variety most (55%) followed by Sumi (18%). Remaining 3 varieties were chosen less than 10%. When leafminers choose and move toward the preferred variety, movement speed was faster. In the plant choice test in the laboratory, as expected, Ghowon was the most chosen but the choice of the other 4 varieties was not different. The same pattern as found in the plant choice test conducted in the greenhouse. Feeding frequency was the highest in Ghowun variety followed by Sumi > Daeri = Seohong > Chubaek in the laboratory test but Ghowun > Sumi > Chubaek > Daeri = Seohong in greenhouse test. When measured in 2 weeks after release, plant damage rates and number of mines were in congruence to the preference results. Further study is needed to clarity the factor responsible for the choice of the plants and also the ways to manage this new pest.

      • Leafminer fly Liriomyza sp. (Diptera: Agromyzidae) and Their Natural Enemies in Potato Crop in Korea

        Rameswor Maharjan,Chuleui Jung 한국응용곤충학회 2012 한국응용곤충학회 학술대회논문집 Vol.2012 No.10

        Liriomyza leafminers comprise a pest group that causes both considerable economic losses and serious quarantine problems. In recent years, the pest status of Liriomyza sp. has changed in Korea, as well as other areas of the world. Potato field survey was conducted in 157 farmers’ field from five provinces during 2011 and 2012. Foliar insect monitoring was done within 5 minutes walking distance by visual inspection. Leafminer fly and parasitoids were collected by sweeping net (10 sweeps/field) in same field in each time. Overall, potato insect species collected constituted of 7 orders, 22 families and 42 species. Aphis gossypii was most abundant species in all the locations. However, Potato tuber moth, Pthorimaea operculella was only dominated species in Boesong (Jellanam-do). L. huidobrensis was the first record infesting the potato in Korea. Liriomyza huidobrensis was only found from Miryang (Gyeongsangnam-do) and Goreyong (Gyeongsangbuk-do). There is a rich complex of parasitoid species; 18 species of parasitoids were recorded. Most parasitoid species (11) belong to the Eulophidae family. The most abundant species were the ectoparasitoid, Diglyphus isaea and endoparasitoid, Ganaspidiumsp. The number and diversity of parasitoid species in potato crop in Korea provides insight into the development of integrated management strategies of leafminer pests.

      • Trap cropping for Management of bugs of mungbean, Vigna radiata (L.) Wilczek. : Trap crop in Nepal.

        Rameswor Maharjan,Chuleui Jung 한국응용곤충학회 2011 한국응용곤충학회 학술대회논문집 Vol.2011 No.10

        Mungbean is an important crop in Nepal. Heteropteran bugs, Nezara virudula, Dolycoris baccurum, and Melanacanthus scutellaris are significantly influencing qualitative and quantitative loss in soybean and mungbean. Though pesticide application is common practice for management of these bugs, pesticide avoidance by the bugs stalls proper management. As an alternative, trap cropping with variety mostly preferred by the bugs as trap crops along with the main crop is considered. Selection of most preferred mungbean variety was performed with six (VC 6173 A; VC 6153 B-20G; VC 3960 A-88; Kalyan; Pratikchha; Saptari local) relative to Saptari local variety. Significant difference was recorded in number of bugs occurrence among the six varieties with maximum number of bugs in VC 6173 (3 bugs/plant) which showed lowest yield. For application, three different trap crop designs were employed in the field. There was significant difference between the main crop i.e. variety Pratiksha and the variety VC6173A used as trap crop in two designs. In conclusion VC6173A is a probable trap crop and the two designs can be employed in the field.

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