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- 저자 : Rajawat, (검색결과 3 건)
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Disease diagnostics using hydrodynamic flow focusing in microfl uidic devices: Beyond flow cytometry
Aakash Rajawat,Siddhartha Tripathi 대한의용생체공학회 2020 Biomedical Engineering Letters (BMEL) Vol.10 No.2
The multi-disciplinary fi eld of microfl uidics has the potential to provide solutions to a diverse set of problems. It off ers theadvantages of high-throughput, continuous, rapid and expeditious analysis requiring minute quantities of sample. However,even as this fi eld has yielded many mass-manufacturable and cost-effi cient point-of-care devices, its direct and practicalapplications into the fi eld of disease diagnostics still remain limited and largely overlooked by the industry. This reviewfocuses on the phenomenon of hydrodynamic focusing and its potential to materialize solutions for appropriate diagnosisand prognosis. The study aims to look beyond its intended cytometric applications and focus on unambiguous disease detection,monitoring, drug delivery, studies conducted on DNA and highlight the instances in the scientifi c literature that haveproposed such approach.
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Koutu, Vaibhav,Rajawat, Shweta,Shastri, Lokesh,Malik, M.M. Techno-Press 2019 Advances in nano research Vol.7 No.4
The present research work reports in-vitro anti-cancer activity of biologically synthesized ZnO nanoparticles (ZnO NPs) against human carcinoma cells viz SCC-40, SK-MEL-2 and SCC-29B using Sulforhodamine-B (SRB) Assay. ZnO NPs were synthesized by a unique and novel biological route using Temperature-gradient phenomenon where the extract of combination of Catharanthus roseus (L.) G. Don (C. roseus), Azadirachta indica (A. indica), Ficus religiosa (F. religiosa) and NaOH solution were used as synthesis medium. The morphology of the ZnO NPs was characterized by Transmission Electron Microscopy (TEM). TEM images reveal that particle size of the samples reduces from 76 nm to 53 nm with the increase in reaction temperature and 68 nm to 38 nm with the increase in molar concentration of NaOH respectively. XRD study confirms the presence of elements and reduction in crystallite size with increase in reaction temperature and NaOH concentration. The diffraction peaks show broadening and a slight shift towards lower Bragg angle ($2{\theta}$) which represents the reduction in crystallite size as well as presence of uniform strain. The FTIR spectra of the extract show transmittance peak fingerprint of Zn-O bond and presence of bioactive molecules These NPs exhibit inhibition greater than 50% for SCC-40, SK-MEL-2 and SCC-29B cell lines and more than 50% cell kill for SCC-29B cells at concentrations < $80{\mu}g/ml$. Nanoparticles with smallest size have shown better anti-cancer activity and peculiar cell-selectivity. The combination of extracts of these plants with ZnO NPs can be used in targeted drug delivery as an effective anti-cancer agent, a potential application in cancer treatment.
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Satya Prakash,Milap Chand Sharma,Rajesh Kumar,P. S. Dhinwa,K. L. N. Sastry,A. S. Rajawat 대한공간정보학회 2016 Spatial Information Research Vol.24 No.6
Land degradation is a major problem in the fragile ecosystem of the Himalayan region. The steep slope with low forest cover and increasing human interference are the major factors of land degradation. Therefore, identification of severe degradation prone areas is necessary for implementing conservation strategies to retard the present rate of degradation processes. The aim of this study is to assess the vulnerable land degradation areas based on Space Application Centre (SAC/ISRO) guidelines and MEDALUS model. Indicators used to calculate the degradation vulnerability are geology, slope, aspect, soil type, rainfall, temperature, land use/land cover, population density, non-worker population and illiteracy. The results reveal that the areas with higher rainfall, less forest cover and large population are highly vulnerable to degradation in spite of moderate slope. The degradation vulnerability index values have been classified into five land degradation categories. The Pong dam is a large water body where the land degradation is nil. The low, moderate, high and very high land degradation categories account for 19.01, 22.79, 31.49 and 17.37% area of the district, respectively.
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