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비닐봉지 재배에 의한 복령 생산 I-환경온도 및 비닐 겹수의 영향
Seong Baek Yang,Hyun Ji Lee,Hyeong Rack Sohn,Seon Man Jeon,Hae Wook Jang,Jeong Hyun Yeum 경북대학교 농업생명과학대학 2015 Current Research on Agriculture and Life Sciences Vol.33 No.2
Poria cocos is an edible and pharmaceutical mushroom with a long history of medicinal use in Korea. For the last 30 years, the domestic cultivated supply of Poria cocos has been unable to meet consumer demand, so Poria cocos is collected in mountainous areas and also imported from China. Thus, to increase the supply of Poria cocos, many artificial cultivation methods have been studied. In this study, Poria cocos is cultivated under different environmental conditions using plastic bags and the results compared. When cultivating Poria cocos at different temperatures (20, 25, 30 and 35oC) and using different numbers of plastic bag layers (1, 2), the most efficient cultivation conditions were a temperature of 25-30oC and 2 plastic bag layers. The fastest growth was at 25-30oC, and the Poria cocos exhibited no weight change when cultivated using layers of plastic bags (1, 2).
배지응고제가 시호캘러스의 Saikosaponin함량에 미치는 영향
성락선,Seong, Rack-Seon 한국생약학회 1996 생약학회지 Vol.27 No.4
In order to product the saikosaponin which is one of the secondary product from Bupleurum falcatum efficiently through the tissue culture, several levels of agar and gellan gum as the gelling agent, 2,4-D as the growth regulator, and L-phenylalanine as the precursor were used with single or combination treatment on MS basal medium. Gellan gum was more effective than agar as the gelling agent in fresh and dry weight increase of callus induced from Bupleurum falcatum leaf segment. Gellan gum medium supplemented with L-phenylalanine produced 1.6 times of fresh weight more than that of agar. The fresh weight was remarkably high in gellan gum when the calli was treated with the combination of 2,4-D and L-phenylalanine similar to the single treatment of 2,4-D or L-phenylalanine. However, the saikosaponin content in callus was high in gellan gum with the single treatment of L-phenylalanine. Especially, the saikosaponin content in gellan gum supplemented with 1.0mg/L L-phenylalanine was 2 times(2.4 mg/g) higher than that in agar medium supplemented with 0.1 mg/L 2,4-D(0.9 mg/g).
L-Phenylalanine이 시호 캘러스의 Saikosaponin 함량에 미치는 영향
성락선,Seong, Rack-Seon 한국생약학회 1996 생약학회지 Vol.27 No.4
This experiment was conducted to find out the effects of L-phenylalanine on the saikosaponin content of callus induced from Bupleurum falcatum leaf segments. In the fresh and dry weight of callus, the addition of 2,4-D than L-phenylalanine was significantly effective. However, the L-phenylalanine treatment rather than 2,4-D was effective for high saikosaponin accumulation in the callus of Bupleurum falcatum.
趙弼衡,成樂宣,裵馨華,蘇雄永,趙德以 全北大學校 基礎科學硏究所 1991 基礎科學 Vol.14 No.2
The study for the quantitative analysis of saikosaponin of Bupleurum falcatum root produced by tissue culture was attempted. The optimal concentration of 2, 4-D was 0.1㎎/l for inducing the callus. The induction and growth of the adventitious root from the callus was obtained in the suspension culture containing MS basal medium supplemented with no plant growth regulators. The results of the quantitative analysis of saikosaponins by high performance liquid chromatography (HPLC) showed that the content of saikosaponin a and c was high in the one-year-old root from somatic embryos and that of saikosaponin d was remarkably high in three-months-old adventitious root from callus.
장승엽,성락선,강신정,이춘길,하광원,류점호,이경순 식품의약품안전청 1998 식품의약품안전청 연보 Vol.2 No.-
카르두스 마리아누스의 조직배양에 있어서 식물생장조절물질에 따른 캘러스의 형성과 캘러스 증식에 미치는 아미노산의 영향을 조사하였다. 그 결과 캘괴스의 유도를 위하써는 2,4-D 0.Img/f에 kiBetin 1.Omg/j 조합처리가가장효과적이었다. 아미노산의 처리에 파른 캘러스의 증꼭상태는 L-phenylalanine>L-leucine)L-valine=L-thrrf nine의 순으로 캘러스 증식이 잘 되었다. 특히 L-phenylalanine은 어느 농도에서도 캘러스 증식이 잘 되었으며 Of mg/스과 농도에서 가장 활발하였다. 아미노산의 처리를 따른 캘러스에서의 silybin 및 silymarin의 성분함량을조각한 결파 L-phenylalanine 0.Smg/f의 농도에서 silybiii의 함량이 0.19mg/g으로써 가장 높게 나타났다. Tissue culture was accomplished to j;nvestigate the effects of antino acids on callus proliferation of fifroun wtarianuuL The callus induction frein the leaf was most effective iu combinationtreatment with 0.Img/f 2.4-D and 1.emg/j kinetin. The callus proliferation was most effective in the me-dium supplemented with 0.Img/f 2,4-D and 0-Smg/f L-phenylalanine. In this condition, the content ofsilybin in callus was 0.19mg/g.
Utilization of Real-Time PCR to Detect Rangifer Cornu Contamination in Cervi Parvum Cornu
Young Hun Shim,Rack Seon Seong,Dong Sup Kim,Shin Jung Kang,Seung Yeup Chang,김형진 대한약학회 2011 Archives of Pharmacal Research Vol.34 No.2
Cervi Parvum Cornu (CPC) is a well-known ethnopharmacological source, whereas Rangifer Cornu (RC) is not considered to be a major source. CPC is distributed in sliced form. Addition of RC to CPC has become an issue in CPC distribution because the appearance of sliced RC is not different from sliced CPC. Therefore, a real-time polymerase chain reaction (PCR) method was developed in this study to detect contaminating RC in CPC. The C-VIC and R-FAM primer/probe sets were designed to specifically amplify CPC and RC DNA, respectively. The specificities and sensitivities of real-time PCR using two primer/probe sets and the applicability of the real-time PCR to powder mixtures, which involved mixtures of powdered CPC and powdered RC in diverse ratios, were evaluated. Real-time PCR using C-VIC and R-FAM primer/probe sets specifically and sensitively amplified both CPC and RC DNA. Furthermore, real-time RCR sensitively detected RC DNA in the powder mixtures of CPC and RC. These results indicate that this real-time PCR method using two primer/probe sets is sufficiently applicable for the detection of contaminant RC in CPC.