Clinicopathologic Characteristics and Prognoses for Multicentric Occurrence and Intrahepatic Metastasis in Synchronous Multinodular Hepatocellular Carcinoma Patients

Li, Shi-Lai,Su, Ming,Peng, Tao,Xiao, Kai-Yin,Shang, Li-Ming,Xu, Bang-Hao,Su, Zhi-Xiong,Ye, Xin-Ping,Peng, Ning,Qin, Quan-Lin,Chen, De-Feng,Chen, Jie,Li, Le-Qun Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.1

Background: Hepatocellular carcinoma (HCC) is one of the most common cancers worldwide, and the outcomes for patients are still poor. It is important to determine the original type of synchronous multinodular HCC for preoperative assessment and the choice of treatment therapy as well as for the prediction of prognosis after treatment. Aims: To analyze clinicopathologic characteristics and prognoses in patients with multicentric occurrence (MO) and intrahepatic metastasis (IM) of synchronous multinodular hepatocellular carcinoma (HCC). Methods: The study group comprised 42 multinodular HCC patients with a total of 112 nodules. The control group comprised 20 HCC patients with 16 single nodular HCC cases and 4 HCC cases with a portal vein tumor emboli. The mitochondrial DNA (mtDNA) D-loop region was sequenced, and the patients of the study group were categorized as MO or IM based on the sequence variations. Univariate and multivariate analyses were used to determine the important clinicopathologic characteristics in the two groups. Results: In the study group, 20 cases were categorized as MO, and 22 as IM, whereas all 20 cases in the control group were characterized as IM. Several factors significantly differed between the IM and MO patients, including hepatitis B e antigen (HBeAg), cumulative tumor size, tumor nodule location, cirrhosis, portal vein and/or microvascular tumor embolus and the histological grade of the primary nodule. Multivariate analysis further demonstrated that cirrhosis and portal vein and/or microvascular tumor thrombus were independent factors differentiating between IM and MO patients. The tumor-free survival time of the MO subjects was significantly longer than that of the IM subjects ($25.7{\pm}4.8$ months vs. $8.9{\pm}3.1$ months, p=0.017). Similarly, the overall survival time of the MO subjects was longer ($31.6{\pm}5.3$ months vs. $15.4{\pm}3.4$ months, p=0.024). The multivariate analysis further demonstrated that the original type (p=0.035) and Child-Pugh grade (p<0.001) were independent predictors of tumor-free survival time. Cirrhosis (p=0.011), original type (p=0.034) and Child-Pugh grade (p<0.001) were independent predictors of overall survival time. Conclusions: HBeAg, cumulative tumor size, tumor nodule location, cirrhosis, portal vein and/or microvascular tumor embolus and histological grade of the primary nodule are important factors for differentiating IM and MO. MO HCC patients might have a favorable outcome compared with IM patients.

Radioactive gas diffusion simulation and inhaled effective dose evaluation during nuclear decommissioning

Li-qun Yang,Yong-kuo Liu,Min-jun Peng,Abiodun Ayodeji,Zhi-tao Chen,Ze-yu Long 한국원자력학회 2022 Nuclear Engineering and Technology Vol.54 No.1

During the decommissioning of the nuclear facilities, the radioactive gases in pressure vessels may leakdue to the demolition operations. The decommissioning site has large space, slow air circulation, andmany large nuclear facilities, which increase the difficulty of workers' inhalation exposure assessment. Inorder to dynamically evaluate the activity distribution of radionuclides and the committed effective dosefrom inhalation in nuclear decommissioning environment, an inhalation exposure assessment methodbased on the modified eddy-diffusion model and the inhaled dose conversion factor is proposed in thispaper. The method takes into account the influence of building, facilities, exhaust ducts, etc. on thedistribution of radioactive gases, and can evaluate the influence of radioactive gases diffusion on workersduring the decommissioning of nuclear facilities.

The Breed and Sex Effect on the Carcass Size Performance and Meat Quality of Yak in Different Muscles

Li Zhang,Baozhong Sun,Qun-li Yu,Qiumei Ji,Peng Xie,Haipeng Li,Li Wang,Yuchun Zhou,Yongpeng Li,Caixia Huang,Xuan Liu 한국축산식품학회 2016 한국축산식품학회지 Vol.36 No.2

The carcass size performances and the meat quality of Gannan and Sibu yak were determined using M. supraspinatus (SU), M. longissimus thoracis (LT) and M.quadriceps femoris (QF). It is found that Sibu yak had significantly higher carcass weight (CW) than Gannan yak with difference of nearly 40 kg, as well as significantly higher eye muscle area (EMA), carcass thorax depth (CTD), round perimeter (RP), etc. The carcass performances of steer yak were significantly higher than heifer yak except meat thickness at round (MTR) (p<0.05). The results show that both yak breed and gender had significant effects on carcass performances. It could be seen that the variation of carcass size performances from breeds is as large as from gender (50.22% and 46.25% of total variation, respectively) through principal component analysis (PCA). Sibu yak had significantly higher L*, b*, WBSF, cooking loss and Fat content, while Gannan yak had significantly higher a*, press loss, protein content and moisture (p<0.05). Yak gender and muscle had insignificant effects on meat colour and water holding capacity (p>0.05). The variation of meat quality of yak from breed is up to 59.46% of total variation according to PCA. It is shown that the difference between breeds, for Gannan yak and Sibu yak, plays an important role in carcass size performance and meat quality.

Identification and characterization of differentially expressed miRNAs between bamboo shoot and rhizome shoot

Qun-Ying Jin,Hua-Zheng Peng,Er-Pei Lin,Nan Li,Dan-Ni Huang,Yan-Li Xu,Xi-Qi Hua,Kui-Hong Wang,Tang-Jun Zhu 한국식물학회 2016 Journal of Plant Biology Vol.59 No.4

As one of the largest members of Poaceae family, bamboo is a very important agricultural plant in the world. The development of bamboo shoot is very special and particularly significant to bamboo production. Understanding the developmental differences between bamboo shoot and rhizome shoot is extremely valuable for us to further elucidate the mechanism of bamboo shoot formation since both bamboo shoot and rhizome shoot develop directly from rhizome bud underground. In this paper, miRNA chips with 413 miRNA probes were used to compare miRNA expressions between bamboo shoot and rhizome shoot. The experiment revealed 64 bamboo shoot upregulated and 56 rhizome shoot up-regulated miRNAs which were classified into four major categories according to deep sequencing based target prediction. Meristem and morphological development related miRNAs were most important in bamboo shoot, especially miR171 and miR156 members. While in rhizome shoot the mainstream of miRNA expressions was metabolism and nutrition related ones, especially miR395 members. The meristem and morphological development related miRNAs in bamboo shoot showed some embryonic characteristics and suggested the participation of several phytohormones like gibberellin, cytokinin and auxin, which were absent in those miRNAs of rhizome shoot. Further qRT-PCR detections of 21 up-regulated miRNAs in bamboo seedlings indicated that 12 ones were regulated to varying degrees by some environmental factors. Among them, rhizome shoot upregulated osa-miR395b was the most environment-sensitive miRNA, particularly to dehydration. And the bamboo shoot up-regulated osa-miR399j proved uniquely and strongly induced by phosphor. The existence of multiple regulation sites from same miRNA suggested the probability of crosstalks among meristem development, metabolism and stress response during bamboo shoot and rhizome shoot development.

Predicament, Opportunity and Path: Reflections on the Development of Tai Chi in the New Era

LI Qun,FENG CHAO-Hai,LEI Tao,WU Zh-iheng,SU Jian-jiao 아시아건강운동학회 2021 Journal of Asian Society for Health & Exercise Vol.3 No.2

The new era has entrusted the era mission of high-quality development of Tai Chi. Standing in a new historical position, this thesis uses the method of literature and logical analysis to explore the realistic difficulties, opportunities of the times and breakthrough paths in the development of Tai Chi, which is a reflection on the breakthrough of the development of it. The research believes that the development of Tai Chi in the new era has the predicament of slow domestic and international development, the gradual dissipation of martial arts functions, and the gradual weakening of cultural attributes. However, the new era has also brought a new opportunity for the Tai Chi movement to have a clearer development blueprint, a more stable social foundation, and a healthy concept deeply rooted in the hearts of the people. The development of Tai Chi in the new era should be guided by a new pattern of domestic development and international promotion. The development of Tai Chi in the new era should be guided by the construction of a new dual-cycle pattern of domestic development and international promotion, the strategy of coordinating the balance of Tai Chi sports competition and fitness functions, and the means of the integration of school education and cultural exchange, so as to realize the high-quality development of Tai Chi.

Knockout of the EgriBLOS2 gene results in the transparent integuments of Ectropis grisescens larvae

Li Jia-Li,Zhuang Xiang-Lin,Yuan Ting-Ting,Cai Xiao-Ming,Luo Zong-Xiu,Bian Lei,Chen Zong-Mao,Li Zhao-Qun,Liu Nai-Yong 한국응용곤충학회 2022 Journal of Asia-Pacific Entomology Vol.25 No.1

The body colors of insects have evolved specialized roles in body protection, signaling and physiology. In some case, the larvae can camouflage their body colors to adapt the habitants and protect themselves. However, the genetic and molecular basis on larval body colors of the tea geometrid, Ectropis grisescens, remains poorly known. Here, we reported an effect of the lysosome-related organelles complex-1, subunit 2 (EgriBLOS2) gene knockout on larval integuments of E. grisescens, by using a clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) system. Combining transcriptomic data and PCR approaches, we first identified the EgriBLOS2 gene from E. grisescens, which encoded 137 amino acids and comprised 3 introns. In the phylogenetic analysis, EgriBLOS2 clustered in the Lepidoptera clade with high conservation to members in other lepidopteran species. Developmental expression profiles revealed that EgriBLOS2 was constantly tran scribed at each stage, in which its expression was significantly lower in 2nd instar larvae than that of other instars. After injecting EgriBLOS2-specific guide RNA and Cas9 messenger RNA into eggs, 70% of larvae showed the translucent integuments in G0 generation, with an emphasis on black splayed patterns in the 2nd and 8th segments of abdomens. However, some typical characteristics of larvae were not obviously changed in mutant instars, such as ocelli, mouthparts and other appendants. This study has unraveled the roles of EgriBLOS2 in the formation of larval integument colors, and provides an alternative strategy for pest management based on the colors in this species.

Crocetin Induces Cytotoxicity in Colon Cancer Cells Via p53-independent Mechanisms

Li, Cai-Yan,Huang, Wen-Feng,Wang, Qun-Li,Wang, Fan,Cai, E.,Hu, Bing,Du, Jia-Cheng,Wang, Jing,Chen, Rong,Cai, Xiao-Jing,Feng, Jing,Li, Hui-Hui Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.8

Objective: Crocin has been proposed as a promising candidate for cancer chemoprevention. The purpose of this investigation was to investigate the chemopreventive action and the possible mechanisms of crocin against human colon cancer cells in vitro. Methods: Cell proliferation was examined using MTT assay and the cell cycle distribution fractions were analyzed using fow cytometric analysis after propidium iodide staining. Apoptosis was detected using theTUNEL Apoptosis Detection Kit with laser scanning confocal microscope. DNA damage was assessed using the alkaline single-cell gel electrophoresis assay, while expression levels of p53, cdk2, cyclinA and P21 were examined by Western blot analysis. Results: Treatment of SW480 cells with crocetin (0.2, 0.4, 0.8 mmol/L) for 48 h signifcantly inhibited their proliferation in a concentration-dependent manner. Crocetin (0.8 mmol/L) signifcantly induced cell cycle arrest through p53-independent mechanisms accompanied by P21 induction. Crocetin (0.8 mmol/L) caused cytotoxicity in the SW480 cells by enhancing apoptosis and decreasing DNA repair capacity in a time-dependent manner. Conclusions: This report provides evidence that crocetin is a potential anticancer agent, which may be used as a chemotherapeutic drug.

Changes of Shoulder, Elbow, and Wrist Stiffness Matrix Post Stroke

Li-Qun Zhang,Jongsang Son,Hyung-Soon Park,Sang Hoon Kang,Yunju Lee,Yupeng Ren IEEE 2017 IEEE transactions on neural systems and rehabilita Vol.25 No.7

<P>Stroke affects multiple joints in the arm with stereotypical patterns of arm deformity involving the shoulder, elbow, wrist, and hand and with disrupted coordination of multiple joints in active movements. However, there is a lack of systematicmethods to evaluatemulti-joints and multi-degree of freedoms (DOF) neuro-mechanical changes, especially for complex systemswith three ormore joints/ DOFs involved. This paper used a novel systematic method to characterize dynamics and control of the shoulder, elbow, and wrist of the human arm individually and simultaneously, including the couplings across themultiple joints during controlled movements. A novel method was developed to decompose the complex system into manageable single-joint level for more reliable characterizations. The method was used in clinical studies to characterize the multi-joint changes associated with spastic impaired arm of 11 patients post stroke and 12 healthy controls. It was found that stroke survivors showed not only increased stiffness at the individual joints locally but also significantly higher couplings across the joints. The relative increases in couplings are often higher than that of the local joint stiffness. The multi-joint characterization provided a tool to characterize impairment of individual patients, which would allow more focused impairment-specific treatment. In general, the decomposition method can be used for even more complex systems, making characterization of intractable system dynamics of three or more joints/DOFs manageable.</P>

Influence of the Interface Property on the Memory Function in a CER Cell Based on Pr0.7Ca0.3MnO3 Films

Qun Wang,김형국,Li Dong Chen,Xiao Min Li,황윤회 한국물리학회 2007 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.51 No.2I

Two kinds of colossal electroresistance (CER) cells, Ag/Pr$_{0.7}$Ca$_{0.3}$MnO$_{3}$/Pt and In/Pr$_{0.7}$ Ca$_{0.3}$MnO$_{3}$/Pt, were prepared to study the memory function. Different reversible-resistance-switching phenomena were observed, depending on the applied electrodes. The I-V characteristics of the two cells have opposite hysteresis properties. The power consumptions and the threshold voltages were also investigated. In particular, the Ag/Pr$_{0.7}$Ca$_{0.3}$MnO$_{3}$/Pt cell with an Ohmic contact interface exhibited better resistance switching retention and small power consumption. In contrast, the In/Pr$_{0.7}$Ca$_{0.3}$MnO$_{3}$/Pt cell with a Schottky contact interface exhibited poor resistance switching retention and large power consumption. The results suggest that the CER cell with an Ohmic contact is better for the resistance memory function.

A Fast Gamma-Ray Dose Rate Assessment Method for Complex Geometries Based on Stylized Model Reconstruction

Li-qun Yang,Yong-kuo Liu,Min-jun Peng,Meng-kun Li,Nan Chao 한국원자력학회 2019 Nuclear Engineering and Technology Vol.51 No.5

A fast gamma-ray dose rate assessment method for complex geometries based on stylized modelreconstruction and point-kernel method is proposed in this paper. The complex three-dimensional (3D)geometries are imported as a 3DS format file from 3dsMax software with material and radiometric attributes. Based on 3D stylized model reconstruction of solid mesh, the 3D-geometrical solids are automaticallyconverted into stylized models. In point-kernel calculation, the stylized source models aredivided into point kernels and the mean free paths (mfp) are calculated by the intersections betweenshield stylized models and tracing ray. Compared with MCNP, the proposed method can implementcomplex 3D geometries visually, and the dose rate calculation is accurate and fast.