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ZNF424, a novel human KRAB/C2H2 zinc finger protein, suppresses NFAT and p21 pathway
( Yue Qun Wang ),( Jun Mei Zhou ),( Xiang Li Ye ),( Yong Qi Wan ),( Yong Qing Li ),( Xiao Yan Mo ),( Wu Zhou Yuan ),( Yan Yan ),( Na Luo ),( Ze Qun Wang ),( Xiong Wei Fan ),( Yun Deng ),( Xiu Shan Wu 한국생화학분자생물학회 (구 한국생화학회) 2010 BMB Reports Vol.43 No.3
Zinc finger-containing transcription factors are the largest single family of transcriptional regulators in mammals, which play an essential role in cell differentiation, cell proliferation, apoptosis, and neoplastic transformation. Here we have cloned a novel KRAB-related zinc finger gene, ZNF424, encoding a protein of 555aa. ZNF424 gene consisted of 4 exons and 3 introns, and mapped to chromosome 19p13.3. ZNF424 gene was ubiquitously expressed in human embryo tissues by Northern blot analysis. ZNF424 is conserved across species in evolution. Using a GFP-labeled ZNF424 protein, we demonstrate that ZNF424 localizes mostly in the nucleus. Transcriptional activity assays shows ZNF424 suppresses transcriptional activity of L8G5-luciferase. Overexpression of ZNF424 in HEK- 293 cells inhibited the transcriptional activity of NFAT and p21, which may be silenced by siRNA. The results suggest that ZNF424 protein may act as a transcriptional repressor that suppresses NFAT and p21 pathway to mediate cellular functions. [BMB reports 2010; 43(3): 212-218]
MiR-675 Promotes the Growth of Hepatocellular Carcinoma Cells Through Cdc25A Pathway
Yu, Ya-Qun,Weng, Jun,Li, Shu-Qun,Li, Bo,Lv, Jun Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.8
Background: MicroRNAs (miRNAs) have fundamental roles in tumorigenesis. MiR-675 is upregulated in hepatocellular carcinoma(HCC) cells. However, the roles of miR-675 in hepatocellular carcinogenesis are still not fully elucidated. In this study, we focus on investigating the effect and mechanism of miR-675 in proliferation of HCC cells. Materials and Methods: The cell proliferation was measured by MTT assays after transfection with miR-675 inhibitor and miR-675 mimics in HCC cells. The expression level of miR-675 was detected by real-time quantitative reverse transcription polymerase chain reaction. Protein expression of Cdc25A was measured by western blotting analysis. Results: In MTT assays, overexpression of miR-675 promoted the proliferation of HCC cells(P<0.05. at 48 hours, P<0.01. at 72 hours) compared with the miR-675mimics control group. Downexpression of miR-675 inhibited the proliferation of HCC cells(P<0.05. at 48 hours, P<0.01. at 72 hours) compared with the miR-675inhibitor control group. In western blotting analysis, the expression level of Cdc25A was significantly increased (p<0.05) after treatment with miR-675 mimics. The expression level of Cdc25A was significantly decreased (p<0.05) after treatment with miR-675 inhibitor. Conclusions: Our results indicate that miR-675 promotes the proliferation in human hepatocellular carcinoma cells by associating with Cdc25A signaling pathway.
Maximum Likelihood and Signal-Selective TDOA Estimation for Noncircular Signals
Fei Wen and Qun Wan,Qun Wan 한국통신학회 2013 Journal of communications and networks Vol.15 No.3
This paper addresses the issue of time-difference-ofarrival (TDOA) estimation for complex noncircular signals. First,under the wide-sense stationary assumption, we derive the maximum likelihood (ML) estimator and the Cramer-Rao lower bound for Gaussian noncircular signals in Gaussian circular noise. The ML estimator uses the second-order statistics information of a noncircular signal more comprehensively when compared with the cross-correlation (CC) and the conjugate CC estimators. Further,we present a scheme to modify the traditional signal-selective TDOA methods for noncircular signals on the basis of the cyclostationarity of man-made signals. This scheme simultaneously exploits the information contained in both the cyclic cross-correlation (CCC) and the conjugate CCC of a noncircular signal.
( Qun Lei ),( Yueyun Chen ) 한국인터넷정보학회 2015 KSII Transactions on Internet and Information Syst Vol.9 No.1
In cognitive radio networks (CRNs), hybrid overlay and underlay sharing transmission mode is an effective technique for improving the efficiency of radio spectrum. Unlike existing works in the literature, where only one secondary user (SU) uses overlay and underlay modes, the different transmission modes should be allocated to different SUs, according to their different quality of services (QoS), to achieve the maximal efficiency of radio spectrum. However, hybrid sharing mode allocation for heterogeneous services is still a challenge in CRNs. In this paper, we propose a new resource allocation method for hybrid sharing transmission mode of overlay and underlay (HySOU), to achieve more potential resources for SUs to access the spectrum without interfering with the primary users. We formulate the HySOU resource allocation as a mixed-integer programming problem to optimize the total system throughput, satisfying heterogeneous QoS. To decrease the algorithm complexity, we divide the problem into two sub-problems: subchannel allocation and power allocation. Cutset is used to achieve the optimal subchannel allocation, and the optimal power allocation is obtained by Lagrangian dual function decomposition and subgradient algorithm. Simulation results show that the proposed algorithm further improves spectrum utilization with a simultaneous fairness guarantee, and the achieved HySOU diversity gain is a satisfactory improvement.
Effects of N- and N-In Doping on ZnO Films Prepared by Using Ultrasonic Spray Pyrolysis
Qun Wang,박세정,신동명,김형국,황윤회,Yiwen Zhang,Xiaomin Li 한국물리학회 2014 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.65 No.11
The effects of N-doping, and N-In co-doping on ZnO films were studied by analyzing the structural,electrical, and optical properties of the films prepared by using an ultrasonic spray pyrolysis(USP) method. According to scanning electron microscopy (SEM) data, all films had very complexsurface structures. Their polycrystallinity were also proven by using an X-ray diffraction method. The Hall-effect measurement showed that both the undoped and the N-doped ZnO films exhibitedn-type conductivity and that the N-In co-doped ZnO film showed p-type conductivity. In the extendedX-ray absorption fine structure (EXAFS) analysis, the number of oxygen atoms in the N-Incodoped ZnO films was found to be larger than that in the N-doped and the undoped ZnO films. The photoluminescence spectra also showed that the N-In co-doping suppressed the concentrationof oxygen vacancies in the ZnO films. Through an effective incorporation of indium atoms, moreoxygen atoms seem to have been introduced into the lattice of the N-In co-doped ZnO films.
Electrical signal characteristics of conductive asphalt concrete in the process of fatigue cracking
Qun Yang,Ping Wang,Xu Li,Hong-Wei Zhang 국제구조공학회 2014 Smart Structures and Systems, An International Jou Vol.14 No.3
As a kind of intelligent materials, conductive asphalt concrete has a broad application prospect including melting ice and snow on the pavement, closing cracks in asphalt concrete, sensing pavement damage, and so on. Conductive pavement will be suffered from fatigue failure as conventional pavement in the process of service, and this fatigue damage of internal structure can be induced by electrical signal output. The characteristics of electrical signal variation of conductive asphalt concrete in the process of fatigue cracking were researched in this paper. The whole process was clearly divided into three stages according to resistance changes, and the development of fatigue damage wasn\'t obvious in stage I and stage II, while in stage III, the synchronicity between the resistance and damage began to appear. Thus, fatigue damage variable D and initial damage value D0 represented by the functions of resistance were introduced in stage III. After calculating the initial damage value D0 under different stress levels, it was concluded that the initial damage value D0 had no noticeable change, just ranged between 0.24 and 0.25. This value represented a critical point which could be used to inform the repair time of early fatigue damage in the conductive asphalt pavement.
( Qun Xia Li ),( Hong Lian Zhang ),( Yi Shan Lu ),( Jia Cai ),( Bei Wang ),( Ji Chang Jian ) 한국미생물 · 생명공학회 2015 Journal of microbiology and biotechnology Vol.25 No.3
Nocardia salmonicida is one of the main pathogens of fish nocardiosis. The purpose of this study was to build a loop-mediated isothermal amplification (LAMP) method for the rapid and sensitive detection of N. salmonicida. A set of four primers were designed from the 16S-23S rRNA intergenic spacer region of N. salmonicida, and conditions for LAMP were optimized as incubating all the reagents for 60 min at 64°C. LAMP products were judged with agar gel electrophoresis as well as with the naked eye after the addition of SYBR Green I. Results showed the sensitivity of the LAMP assay was 1.68 × 103 CFU/ml (16.8 CFU per reaction) and 10-fold higher than that of PCR. The LAMP method was also effectively applied to detect N. salmonicida in diseased fish samples, and it may potentially facilitate the surveillance and early diagnosis of fish nocardiosis.
Static and Cyclic Tension Behavior of Post-Installed Rebar after Exposure to High Temperature
Qun Xie,Song Xue,Yongqiang Zhao 대한토목학회 2020 KSCE JOURNAL OF CIVIL ENGINEERING Vol.24 No.6
In order to investigate the static and cyclic tension behavior of post-installed rebar along with cement-based adhesive that is adopted after exposure to high temperature, a three-phase experimental program has been carried out and two typical embedment depths, 10d and 15dare designed. The surfaces of specimens are firstly heated up to 800oC, and then the residual strength at different temperatures of cement-based adhesive has been studied by consequent static pull-out tests. Experimental results show that the tensile strength has a significant reduction at elevated temperatures compared with that of room temperature. The cement-based adhesive has almost lost its full bond strength when the temperature exceeds 350oC. Finally, the bond performance of cement-based adhesive under cyclic loading has been further tested and analyzed. It is found that both 10d and 15d specimens have failed abruptly with rebar pull-out. The employment of long embedment depth has been verified to be an effective method to improve the bond performance of cement-based adhesive under static and cyclic loading. Static numerical investigation indicates that the bond stress distribution is not uniform along the embedment length of post-installed rebar at different temperatures of cement-based adhesive. The temperature-dependent tensile strength prediction has also been obtained based on the experimental data.