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Significance of $p27^{kip1}$ as potential biomarker for intracellular oxidative status
Quintos, Lesley,Lee, In-Ae,Kim, Hyo-Jung,Lim, Ji-Sun,Park, Ji-A,Sung, Mi-Kyung,Seo, Young-Rok,Kim, Jong-Sang The Korean Nutrition Society 2010 Nutrition Research and Practice Vol. No.
Our previous proteomic study demonstrated that oxidative stress and antioxidant delphinidin regulated the cellular level of $p27^{kip1}$ (referred to as p27) as well as some heat shock proteins in human colon cancer HT 29 cells. Current study was conducted to validate and confirm the regulation of these proteins using both in vitro and in vivo systems. The level of p27 was decreased by hydrogen peroxide in a dose-dependent manner in human colon carcinoma HCT 116 (p53-positive) cells while it was increased upon exposure to hydrogen peroxide in HT 29 (p53-negative) cells. However, high concentration of hydrogen peroxide (100 ${\mu}M)$ downregulated p27 in both cell lines, but delphindin, one of antioxidative anthocyanins, enhanced the level of p27 suppressed by 100 ${\mu}M$ hydrogen peroxide. ICR mice were injected with varying concentrations of hydrogen peroxide, delphinidin and both. Western blot analysis for the mouse large intestinal tissue showed that the expression of p27 was upregulated by 25 mg/kg BW hydrogen peroxide. To investigate the association of p27 regulation with hypoxia-inducible factor 1-beta (HIF-$1{\beta}$), the level of p27 was analyzed in wild-type mouse hepatoma hepa1c1c7 and Aryl Hydrocarbon Nuclear Translocator (arnt, HIF-$1{\beta}$)-defective mutant BPRc1 cells in the absence and presence of hydrogen peroxide and delphinidin. While the level of p27 was responsive to hydrogen peroxide and delphinidin, it remained unchanged in BPRc1, suggesting that the regulation of p27 requires functional HIF-$1{\beta}$. We also found that hydrogen peroxide and delphinidin affected PI3K/Akt/mTOR signaling pathway which is one of upstream regulators of HIFs. In conclusion, hydrogen peroxide and antioxidant delphinidin seem to regulate intracellular level of p27 through regulating HIF-1 level which is, in turn, governed by its upstream regulators comprising of PI3K/Akt/mTOR signaling pathway. The results should also encourage further study for the potential of p27 as a biomarker for intracellular oxidative or antioxidant status.
Significance of p27<SUP>kip1</SUP> as potential biomarker for intracellular oxidative status
Lesley Quintos,In Ae Lee,Hyo Jung Kim,Ji-Sun Lim,Jia Park,Mi-Kyung Sung,Young Rok Seo,Jong-Sang Kim 대한지역사회영양학회 2010 Nutrition Research and Practice Vol.4 No.5
Our previous proteomic study demonstrated that oxidative stress and antioxidant delphinidin regulated the cellular level of p27<SUP>kip1</SUP> (referred to as p27) as well as some heat shock proteins in human colon cancer HT 29 cells. Current study was conducted to validate and confirm the regulation of these proteins using both in vitro and in vivo systems. The level of p27 was decreased by hydrogen peroxide in a dose-dependent manner in human colon carcinoma HCT 116 (p53-positive) cells while it was increased upon exposure to hydrogen peroxide in HT 29 (p53-negative) cells. However, high concentration of hydrogen peroxide (100 μM) downregulated p27 in both cell lines, but delphindin, one of antioxidative anthocyanins, enhanced the level of p27 suppressed by 100 μM hydrogen peroxide. ICR mice were injected with varying concentrations of hydrogen peroxide, delphinidin and both. Western blot analysis for the mouse large intestinal tissue showed that the expression of p27 was upregulated by 25 mg/kg BW hydrogen peroxide. To investigate the association of p27 regulation with hypoxia-inducible factor 1-beta (HIF-1β), the level of p27 was analyzed in wild-type mouse hepatoma hepa1c1c7 and Aryl Hydrocarbon Nuclear Translocator (arnt, HIF-1β)-defective mutant BPRc1 cells in the absence and presence of hydrogen peroxide and delphinidin. While the level of p27 was responsive to hydrogen peroxide and delphinidin, it remained unchanged in BPRc1, suggesting that the regulation of p27 requires functional HIF-1β. We also found that hydrogen peroxide and delphinidin affected PI3K/Akt/mTOR signaling pathway which is one of upstream regulators of HIFs. In conclusion, hydrogen peroxide and antioxidant delphinidin seem to regulate intracellular level of p27 through regulating HIF-1 level which is, in turn, governed by its upstream regulators comprising of PI3K/Akt/mTOR signaling pathway. The results should also encourage further study for the potential of p27 as a biomarker for intracellular oxidative or antioxidant status.
Significance of p27kip1 as potential biomarker for intracellular oxidative status
Lesley Quintos,이인애,김효정,임지선,박지아,성미경,서영록,김정상 한국영양학회 2010 Nutrition Research and Practice Vol.4 No.5
Our previous proteomic study demonstrated that oxidative stress and antioxidant delphinidin regulated the cellular level of p27kip1 (referred to as p27) as well as some heat shock proteins in human colon cancer HT 29 cells. Current study was conducted to validate and confirm the regulation of these proteins using both in vitro and in vivo systems. The level of p27 was decreased by hydrogen peroxide in a dose-dependent manner in human colon carcinoma HCT 116 (p53-positive) cells while it was increased upon exposure to hydrogen peroxide in HT 29 (p53-negative) cells. However, high concentration of hydrogen peroxide (100 μM) downregulated p27 in both cell lines, but delphindin, one of antioxidative anthocyanins,enhanced the level of p27 suppressed by 100 μM hydrogen peroxide. ICR mice were injected with varying concentrations of hydrogen peroxide,delphinidin and both. Western blot analysis for the mouse large intestinal tissue showed that the expression of p27 was upregulated by 25 mg/kg BW hydrogen peroxide. To investigate the association of p27 regulation with hypoxia-inducible factor 1-beta (HIF-1β), the level of p27 was analyzed in wild-type mouse hepatoma hepa1c1c7 and Aryl Hydrocarbon Nuclear Translocator (arnt, HIF-1β)-defective mutant BPRc1 cells in the absence and presence of hydrogen peroxide and delphinidin. While the level of p27 was responsive to hydrogen peroxide and delphinidin, it remained unchanged in BPRc1, suggesting that the regulation of p27 requires functional HIF-1β. We also found that hydrogen peroxide and delphinidin affected PI3K/Akt/mTOR signaling pathway which is one of upstream regulators of HIFs. In conclusion, hydrogen peroxide and antioxidant delphinidin seem to regulate intracellular level of p27 through regulating HIF-1 level which is, in turn, governed by its upstream regulators comprising of PI3K/Akt/mTOR signaling pathway. The results should also encourage further study for the potential of p27 as a biomarker for intracellular oxidative or antioxidant status.
Checklist of Mushrooms of Mt. Cleopatra Needle Forest Reserve in Palawan Island, Philippines
( Dae Ho Kim ),( Nguyen Manh Ha ),( Mutya Ma Quintos Manalo ),( Manuel Baldovino ),( Jong Kyu Lee ) 한국산림과학회(구 한국임학회) 2021 한국산림과학회지 Vol.110 No.2
A mushroom survey was conducted in Mt. Cleopatra Needle Forest Reserve (CNFR) northeast of Puerto Pincesa, the capital city of Palawan island, from August 2018 to September 2019. During this period, 433 fungal fruiting bodies were collected from 3 different from survey sites, Concepcion, Kalakwasan, and Tanabag Barangays. The specimens were identified based on their morphological and molecular analyses and classified into 176 species, 114 genera, 55 families, and 22 orders. The mushrooms belong to Ascomycota were classified into 20 species, 15 genera, 12 families, and 7 orders, while those belongs to Basidiomycota were classified into 156 species, 99 genera, 43 families, and 15 orders, respectively. Among these mushrooms, the families with high frequency were Polyporaceae (18.9%), Marasmiaceae (11.5%), Xylariaceae (9.7%), Agaricaceae (8.3%), Auriculariaceae (4.8%), Ganodermataceae (4.2%), Hypoxylaceae (3.2%), and Sarcoscyphaceae (3.0%), and comprised 63.7% of the total specimens identified. This report may be the first checklist of mushrooms in Mt. CNFR and could be used for developing conservation strategies of the critical habitat in Palawan island.