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Rongbai Li,Jijing Luo,Yongliang Wang,Qigui Jiang,Jianbin Liu,Wei Zeng,Yindi Zeng 한국유전학회 2017 Genes & Genomics Vol.39 No.2
Rice (Oryza sativa L.) is thermophilic crop and all of its growth stages are affected by low temperature stress. However, some populations of common wild rice in Guangxi Province (China) (GXWR) can tolerate low temperature stress. To understand the molecular mechanisms underlying chilling tolerance in these wild rice resources, transcriptome sequencing was performed to study the differentially expressed genes (hereafter referred to as DEGs) between GXWR-derived chilling-tolerant chromosome segment substitution line (hereafter referred to as CSSL), DC90, and its chilling-sensitive recurrent parent 9311 under early chilling stress. Comparative transcriptomic analysis revealed 659 DEGs exclusively identified in DC90 in response to early chilling stress. Functional clustering by CARMO (comprehensive annotation platform for functional exploration of rice multiomics data) showed that majority of the DEGs were enriched in chloroplasts, suggesting a connection between chilling stress tolerance and photosynthesis regulation exists in rice. KEGG analysis of these DEGs presented a complicated chilling responsive regulatory network, including ‘phytohormone signaling’, ‘photosynthesis pathway’, ‘ribosome translation machinery’, ‘phenylpropanoid biosynthesis’, which is coordinately involved in early chilling response. Here, the identified DEGs and unveiled molecular regulatory network sheds light on deep understanding the mechanisms underlying rice chilling stress tolerance. As well, KEGG term-enriched DEGs, chilling tolerant QTLs (quantitative trait loci), and co-localized DEGs in introgression interval, will be focused for further functional investigation of the molecular mechanisms of chilling stress response in rice.
Analysis on Association of a SNP in the Chicken OBR Gene with Growth and Body Composition Traits
Wang, Ying,Li, Hui,Zhang, YuanDan,Gu, ZhiLiang,Li, ZhiHui,Wang, QiGui Asian Australasian Association of Animal Productio 2006 Animal Bioscience Vol.19 No.12
Leptin receptor (OBR) is a member of the class I cytokine receptor family. It signals mainly via the JAK/STAT pathway and plays an important role in regulating body energy storage and metabolism. This study was designed to investigate the effects of the OBR gene on chicken growth and body composition. Broiler lines selected divergently for or against abdominal fat were used. Primers for the exon9-region in the OBR gene were designed using chicken genomic sequences from the public genome domain. A C/A single nucleotide polymorphism (SNP) was found and its three genotypes (AA, AB and BB) were identified in this population. The results showed that the OBR polymorphism was associated with fatness traits, such as abdominal fat weight and abdominal fat percentage. This research suggests that OBR or a linked gene has effect on fat deposition in the chicken.
( Keji Quan ),( Zhuang Zhu ),( Sanjie Cao ),( Fei Zhang ),( Chang Miao ),( Xintian Wen ),( Xiaobo Huang ),( Yiping Wen ),( Rui Wu ),( Qigui Yan ),( Yong Huang ),( Xiaoping Ma ),( Xinfeng Han ),( Qin Z 한국미생물 · 생명공학회 2018 Journal of microbiology and biotechnology Vol.28 No.12
In our previous studies, we have identified several in vivo-induced antigens and evaluated their potential as subunit vaccine candidates in a murine model, in which the recombinant protein GalT showed the most potent immunogenicity and immunoprotective efficacy against Actinobacillus pleuropneumoniae. To exploit a more efficient way of delivering GalT proteins, in this study, we employed the widely studied E. coli outer membrane vesicles (OMVs) as a platform to deliver GalT protein and performed the vaccine trial using the recombinant GalTOMVs in the murine model. Results revealed that GalT-OMVs could elicit a highly-specific, IgG antibody titer that was comparable with the adjuvant GalT group. Significantly higher lymphocyte proliferation and cytokines secretion levels were observed in the GalT-OMVs group. 87.5% and 50% of mice were protected from a lethal dose challenge using A. pleuropneumoniae in active or passive immunization, respectively. Histopathologic and immunohistochemical analyses showed remarkably reduced pathological changes and infiltration of neutrophils in the lungs of mice immunized with GalT-OMVs after the challenge. Taken together, these findings confirm that OMVs can be used as a platform to deliver GalT protein and enhance its immunogenicity to induce both humoral and cellular immune responses in mice.
Zhang, Hui,Wang, Shouzhi,Li, Hui,Yu, Xijiang,Li, Ning,Zhang, Qin,Liu, Xiaofeng,Wang, Qigui,Hu, Xiaoxiang,Wang, Yuxiang,Tang, Zhiquan Asian Australasian Association of Animal Productio 2008 Animal Bioscience Vol.21 No.10
Abdominal fat characters are complex and economically important in the poultry industry. Their selection may benefit from the implementation of marker-assisted selection (MAS). The objective of this study was to identify the markers linked to QTL responsible for fatness traits. The Northeast Agricultural University broiler lines divergently selected for abdominal fat content (NEAUHLF) were used in the study. A total of 596 individuals from the divergent tails from the 6th to the 10th generations were genotyped at 23 microsatellite markers on chromosome 1. The differences of allele frequencies of all marker alleles between the divergent tails across the five generations were recorded. The allele frequencies of five markers, including LEI0209, LEI0146, MCW0036, ADL328 and MCW0115, had significant differences between the two tails in all five generations. The resulting p-values using Fisher's exact test on eleven markers, containing MCW248, MCW0010, MCW0106, LEI0252, LEI0068, MCW0018, MCW0061, LEI0088, MCW200, MCW283 and ROS0025, had a decreasing tendency from the 6th to the 10th generation. Statistical analysis showed that polymorphisms of the eight markers, including LEI0209, LEI0146, ROS0025, MCW0115, MCW0010, MCW0036, MCW283, ADL328, were significantly (p<0.0011) or suggestively (p<0.05) associated with abdominal fat content (AFW and AFP) across generations. It is concluded that the eight markers could be associated with the QTL affecting the deposition of abdominal fat in broiler chickens.