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SHIN, PYONG KYUN,Koo, Ja Hyup,Lee, Woo Jong,SEO, JIN HO 한국화학공학회 1996 Korean Journal of Chemical Engineering Vol.13 No.1
A mathematical model was formulated to describe growth and cloned protein production in the recombinant Escherichia coli cells containing phoA-directed expression systems. Kinetic parameters for the strains with two fusion genes (phoA-lacZ either on the chromosome or on a multicopy plasmid and phoA-amyE on a multicopy plasmid) were estimated and compared to analyze the effects of cloning site (chromosome and plasmid), product type (E. coli β-galactosidase and Bacillus subtilis α-amylase), and culture temperature on the cell's behavior. The presence of a multicopy plasmid reduced the specific growth rate and the phosphate uptake rate of the cell, both by 10%, compared with those of the chromosome-integrated strain. The overexpression of B. subtilis α-amylase decreased the specific growth rate and the glucose consumption rate more than the β-galactosidase overproduction system. The presence of multiple copies of the phoA promoter on either an intact phoA gene or the fusion gene reduced both the repression and derepression efficiencies. Culture temperatures showed a significant effect on α-amylase production. A temperature of 30℃ is more desirable than 37℃ for α-amylase production in the recombinant E. coli containing the phoA promoter.
노병의,전제균 대한물리치료학회 1999 대한물리치료학회지 Vol.11 No.1
A study on severity of cercbro-vascular accident patients in Korea was conducted in order to determine the risk factors affecting the severity of stroke patients. This study was performed by interviewing a total of 477 hospitalized and ambulatory patients of CVA in Seoul, Taejon, Taegu and Pusan areas from April 1, 1998 through June 30, 1998. The results are as follows; 1. Two hundred and sixty seven (56%) of Four hundred and seventy seven subjects were males, and two hundred and ten (44%) were females. 28.3% of the subjects' ages were 50's and 27.3% were 60's and 18.5% were 70's. 22.6% of the subjects' occupations were home makers, 17.8% were farming. 6b.4% of the subjects spent their childhood in urban areas and 33.1% in rural areas. 41.7% of the subjects became victims of CVA in Spring, 35.0% in Winter. 2. On physical and mental conditions of the subjects at the occurrence of CVA, 28.7% of the subjects were engaged in physical activities, 22.6% were in a rest state 19.5% were in sleeping and 18.9% were mentally shocked. 79.4% of the mate subjects and 14.8% of female subjeccts smoked cigarettes. 82% of male subjects drank coffeae. 81.1% of the subjects did not exercise regularly. 45.9% of the subjects had the systolic blood pressures in the range of 160 to 199 ㎜Hg and 5.6% of the subjects had hypertension before the occurrence of stroke and 11.7% had diabetes. 3. Of the types of strokes, cerebral hemorrhage was the highest (49.1%), cerebral infarction was the second (41.1%). Severe strokes were found in cerebral hemorrhage cases (52.0%) and cerebral infarction cases (40.1%). 50.9 of the male subjects were moderate cases, 50.9% were severe cases. In females, moderate cases were 72.4%, severe cases 15.2%. 37.5% of the subjects who had preceding diseases were severe eases, and 15.6% of the subjects without preceding diseases were severe cases. 50.7% of the subjects whose family members had strokes had severe strokes. 34% of the subjects sleeping less than 6 hours a day, 42.4% of the subjects with irregular eating habit, 33.3% of the subjects who liked meats, and 42.3% of the subjects who liked salty foods had severe strokes. 35.9% of the subjects with hot temper, 27.6 of the subjects with moderate temper and 14.8% of subjects with mild temper were severe cases. 4. The correlation coefficient between obesity and blood pressure was 0.094.
Predicting speeding behavior with a car simulator
Yong-Kyun Sihn,Jun-Beom Ryu,Pyong-Nam Lim 한국자동차공학회 2008 한국자동차공학회 Symposium Vol.2008 No.9
Speeding is a major cause of traffic accidents that affects the safety of pedestrians, harms drivers and their families, property and mental health. However, most research on speeding behavior has been conducted by survey, so the relationship between cause and effect is unclear. The reliability of the research cannot be carefully examined because most research involves retrospective methods. It is clear that conducting experimental research is important for overcoming these limitations, but it is impossible to perform tests with real cars in real situations due to the inability to control situational factors (e.g. car traffic, signals) which affect participants’ behavior. A car simulator experiment was designed and the theory of planned behavior was applied to the experiment. Each participant was grouped and assigned to either high speeding or low speeding according to their levels of speeding intention and analyzed the difference between the groups against driving behavior indexes. It was found there was a statistically significant difference between the groups on average driving speed, speeding frequency (highway area), accelerator pressure and total time taken in traveling. A discussion indentifying the limitations and the implications of the results from this study are presented.
담수 및 염수 수송이 은연어의 스트레스 반응에 미치는 영향
전중균(Joong Kyun Jeon),김병기(Pyong Kih Kim),박용주(Yong Joo Park),명정구(Jung Goo Myung),김종만(Jong Man Kim) 한국수산과학회 2000 한국수산과학회지 Vol.33 No.2
To study the stress response of coho salmon (Oncorhynchus kisutch) during transportation, the stress responses of the fish confined in a container box filled with freshwater or 5‰ salt-water were monitored pre and post 10 hours transportation. Changes of cortisol as the first stress indicator, and glucose (GLC), lactate (LAC), triglyceride (TG), cholesterol (CHOL), sodium (Na^+), chloride (Cl^-), alanine aminotransferase (ALT), aspartate aminotransferase (AST) and lactate dehydrogenase (LDH) as the second stress indicators were compared between the fish in two hauling media. Results showed significantly lower levels of cortisol, GLC, LAC, TG, CHOL and AST in salt-water group than freshwater group. It was shown that using salt-water for transportation could lessen the stress level of the coho salmon.