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Cloning and Characterization of Bovine 5-Cytosine DNA Methyltransferase I cDNA
Poongyeon Lee,Kwan-Sik Min,Hyun-Gi Lee,Soon-Jeung Kim,Hee-Kyoung Chung,Myung-Kyu Seo,Yun-Keun Lee,Sung-Woo Kim,Jin-Ki Park,Hwan-Hoo Seong,Moosik Kwon,Won-Kyong Chang 한국동물생명공학회(구 한국동물번식학회) 2003 Reproductive & developmental biology Vol.27 No.1
Cloning and Characterization of Bovine 5-Cytosine DNA Methyltransferase I cDNA
Poongyeon Lee,Kwan-Sik Min,Hyun-Gi Lee,Soon-Jeung Kim,Hee-Kyoung Chung,Myung-Kyu Seo,Yun-Keun Lee,Sung-Woo Kim,Jin-Ki Park,Hwan-Hoo Seong,Moosik Kwon,Won-Kyong Chang 한국동물번식학회 2003 Reproductive & Developmental Biology(Supplement) Vol.27 No.1s
초위성체 표지로 본 한국 재래닭 집단의 분자유전학적 구성
이풍연(Poongyeon Lee),연성흠(Seong-Heum Yeon),김재환(Jae-Hwan Kim),고응규(Yeoung-Gyu Ko),손준규(Jun-Kyu Son),이희훈(Hee-Hoon Lee),조창연(ChangYeon Cho) 韓國家禽學會 2011 韓國家禽學會誌 Vol.38 No.2
초위성체(MS) 표지를이용하여한국재래닭집단의각각의 분자유전학적 특성을 조사하고, 그 평가를 통해 한국 재래닭에 대한 품종 및 계통 분류의 기초를 마련하고자 본 연구를 수행하였다. 또한, 한국 재래닭 집단 내 및 집단간 유전적 변이성을 확인하고, 그 분류 및 특성 평가를 위한 MS 분석 체계를 마련하여 국내 가축유전자원의 관리에 활용코자 하였다. 국내 관리 기관 및 농가 보유 11개 계통의 한국 재래닭 및 상용계 462 수를 대상으로 19개 MS 표지로 분석한 결과, 한국 재래닭 집단은 상용계부터 분자유전학적으로 별개의 집단으로 구분되며, 특히 한국 재래닭 중 긴꼬리닭 계통은 상용계와 국내 토종닭 어느 집단과도 확연히 분리되는 것을 확인하였다. 한국 재래닭 집단 간의 유전거리는 0.11~0.18로 비교적 낮게 나타났으나, 유전적 균일도는 R 계통을 제외하고 0.86~0.88로 코니쉬 계통을 제외한 상용계의 0.95~0.97보다 비교적 낮았다. 다만, 긴꼬리닭 집단의 유전적 균일도는 0.91~0.97로 높게 나타났다. 본 연구를 통하여 한국 재래닭 집단 간의 유전적 차이 및 동질성, 그리고 집단내 의유전적 균일성을 확인하고, 긴꼬리닭 계통의 위치를 확인하였다. 이러한 결과는 국내 유전자원의 고유성을 인정할 수 있는 과학적인 근거로서, 국가 수준의 가축유전자원 평가, 관리의 기초자료로 활용될 수 있을 것이다. The study was conducted to select and optimize microsatellite (MS) markers for evaluate Korean Native Chicken (KNC) breeds in order to provide standard for the classification and breed definition of the indigenous breeds. The study also aimed to characterize and classify each KNC populations for inventory and management of avian genetic resources. A total of 462 chickens from 11 populations of chicken breeds including eight KNC breeds and three commercial chicken breeds were analyzed with 19 MS markers. KNC breeds, especially Long-Tail Chicken breeds, formed separate cluster from those commercial chicken breeds. Genetic distances between KNC populations (0.11~0.18) were relatively shorter. Genetic uniformity of KNC (except KNCR breed) (0.86~0.88) were higher than that of commercial breeds (except Cornish) (0.95~0.97). On the other hand, genetic uniformity of KNC Long Tail (KNCLT) were relatively higher (0.91~0.97). The result can be used to evaluate and manage animal genetic resources at national scale.
Recombinant human erythropoietin produced in milk of transgenic pigs
Park, Jin-Ki,Lee, Yun-Keun,Lee, Poongyeon,Chung, Hak-Jae,Kim, Sungwoo,Lee, Hyun-Gi,Seo, Myung-Kyu,Han, Joo-Hee,Park, Chun-Gyu,Kim, Hun-Taek,Kim, Yong-Kook,Min, Kwan-Sik,Kim, Jin-Hoi,Lee, Hoon-Taek,Cha Elsevier 2006 Journal of biotechnology Vol.122 No.3
<P><B>Abstract</B></P><P>We have developed a line of transgenic swine harboring recombinant human erythropoietin through microinjection into fertilized one cell pig zygotes. Milk from generations F<SUB>1</SUB> and F<SUB>2</SUB> transgenic females was analyzed, and hEPO was detected in milk from all lactating females at concentrations of approximately 877.9±92.8IU/1ml. The amino acid sequence of rhEPO protein in the transgenic pig milk matched that of commercial rhEPO produced from cultured animal cells. In addition, an F-36 cell line, which proliferates in the presence of hEPO or commercial EPO, was induced to synthesize erythroid by extracts from tg sow milk. This study provides evidence that production of purified rhEPO from transgenic pig milk is a potentially valuable technology, and can be used as a cost-effective alternative in clinical applications as well as providing other clinical advantages.</P>
Expression of GFP Gene in the Porcine Preimplantation Embryos after ICSI with DNA/Sperm Complex
Joo-Hee Han,Sungwoo Kim,Poongyeon Lee,Chun-Gyu Park,Hyun-Gi Lee,Boh-Suk Yang,Ki-Hyeong Rhee,Chang-Hyung Lee,Hoon-Taek Lee,Won-Kyong Chang,Jin-Ki Park 한국동물생명공학회(구 한국동물번식학회) 2006 Reproductive & developmental biology Vol.30 No.2
The possibility of producing transgenic embryos expressing the green fluorescence protein (GFP) gene have been evaluated after transfer of exogenous gene into the porcine zygote cytoplasm using the intracytoplasm sperm injection (ICSI) as gene delivery method. For DNA binding to sperm heads, 0.05% Triton X-100 or Lipofectin was used. After injection of the sperm bound to DNA by means of Lipofectin or Triton X-100 triturate, the blastocyst formation rates on day 6 were not significantly different from that of ICSI only group (18.8, 19.2 and 25.3%). In terms of GFP expression, more embryos were in GFP form in Triton X-100 group than in Lipofectin group (40.6 vs 36.4%), while percentage of non-mosaic embryos expressing the GFP gene in all blastomere was higher (P<0.05) in Lipofectin group than in Triton X-100 group (4.2 vs 0.9%). ICSI embryos derived from sperm treated with Lipofectin/DNA complex was transferred into 3 recipients and were collected by uterine flushing on days 5, 7 and 15 after embryo transfer, and then GFP expression was observed by a fluorescence microscopy. Over 26% of the collected embryos were normally expressed GFP gene. These results suggest that foreign gene transfer method with DNA bound sperm caused minimal damage to structure of oocytes that can result to full development of porcine embryos. This was confirmed in this study when the embryos that were transferred after ISCI of DNA bound sperm had a normal development and gene expression until preimplantation.
장시혁,류종석,김재범,이풍연,권무식 성균관대학교 생명과학자원연구소 1996 生命資源科學硏究 Vol.3 No.2
PU.1 is a transcription factor which binds to a DNA motif, 5'-GAGGAA-3' (PU Box). Its expression is restricted to macrophage and B cell in mammal. Activation of the PU.1 transcription factor seems to be accomplished by the phosphorylation of specific serine residue(s) of PU.1 the polypeptide. In an attempt to elucidate the phosphorylation site(s), a PU1 mutant clone named pJKS142A was constructed as follows. Two nucleotides coding for the 142th amino acid [serine(AGC)] of the PU1 polypeptide, "Adenine & Guanine", were substituted by "Guanine & Cytosine" by employing splicing by overlap extenion (SOE) methods. The mutant PU.1 cDNA, PU.S142A, was ligated into HindⅢ/XbaI-cut pBluescript KS+, and then transformed into E. coli XL-1 Blue to generate pJKS142A. Recombinant plasmid DNA, pJKS142A was isolated and the DNA sequence was determined by the method of Sanger to confirm the site directed mutagenesis of PU.1 eDNA. The pJKS142A will be instrumental in evaluating a plusible phosphorylation site(s) of PU.1.