http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
韓國人에서 血友病 B 遺傳子(血液凝固因子 Ⅸ)의 構造 및 制限酵素 Dde Ⅰ, MnI Ⅰ 切片길이 多形性에 關한 分子 遺傳學的 考察
이풍연,전봉균,이정민,권오병,권무식 성균관대학교 생명과학자원연구소 1996 生命資源科學硏究 Vol.2 No.2
Hemophilia B, a human chromosome X-linked recessive disease, is a bleeding disorder resulting from defect or abnormality in blood coagulation factor IX. DNA-based prenatal diagnosis or carrier detection for hemophilia B in Korean has been developed by analyzing restriction fragment length polymorphisms(RFLPs). Two polymorphisms( Dde I and Mnl I) were investigated as follow. Genomic DNAs were extracted from blood of 50 females at the age of twenty. The primers were chemically synthesized by the method of phosphoamidite. Mnl I primers were derived from exon 6, while Dde I primers, from the flanking sequences of intron 1 of the factor IX gene. Genomic DNAs were amplified with Mnl I primers to generate 405 nts long fragments in all cases. They were digested with Mnl I to analyze the polymorphic site on agarose gel. No MnlI polymorphic site was found in all cases. Also, the genomic DNAs were amplified with Dde I primers to generate ca. 320 nts long fragments in all cases. These results are not correlated with those obtained from the Caucasian. It suggests that Korean could exhibit different patterns of Dde I and Mnl I polymorphisms in the gene for blood coagulation factor IX. Direct sequencing of the polymorpic sites will confirm the above sugestion.
정연택,서영수,김연풍 明知大學校 産業技術硏究所 1986 産業技術硏究所論文集 Vol.5 No.-
In this paper, we combine the lead condensor connected on the side of load with the dual 3-pulse converter which has the intermediate-point interphase reactor in the symmetrical phase displacemental transformer, to improve power factor. For the variation of reactive power variable inductor acts as VAR compensator to reduce harmonics, and to improve power factor and waveform.
朴年圭,李海浜,金惠英 동국대학교 자연과학연구소 1986 자연과학연구 논문집 Vol.6 No.-
우리나라 장려 잠품종의 원종 중에서 14품종을 공시하여 주에의 행동반경에 대한 차이와 유전력 및 실용형질과의 관련성을 분석하였다. 행동반응은 유충의 각 시기에 따라서 품종간 차이가 있었으며 대체로 일본계통이 중국 계통에 비하여 민감한 경향이었고 각 품종 공히 행동반응이 가장 민감한 시기는 숙잡기였으며 다음은 개미누에였고 2령부토 5령까지는 상당히 둔한 경향이였다. 행동반경의 추정된 유전력은 유충의 시기별로 차이가 있었으며 h²(%)=52,121~88,411의 범위였으며, 행동발경과 타협질과의 관계에 있어서 행동반경과 생견 1 ㅣ 과수 및 행동반경과 최청부터 발아까지의 기간 사이에서는 "正"의 상관, 행동반경과 고치무게의 시이에서는 "負"의 상관이 인정되었다. The strain differences in larval walking behavior and their heredity in 14 varieties fo silk worm, Bombyx mori, has been investigate. The strain difference of walking response have been responsed in each larval stage and the bahavioral response of Japanse races was more sensitive than that of Chinese races, mostly. During matured silk worm larval stage the walking response was the highest and the next was the larvae hatched newly. Also very low sensitivity appeared from 2nd instar to 5th instar. Heritability of larvar walking distance were different depend on the larval instar the range of heritabilites(%) was 52.121-88.411. There was positive corelation between walking distance and period from incubation to emergence, but negative co relation between walking distance and single cocoon weight.
한국인에서 혈액응고인자 Ⅷ 유전자의 제한효소 절편길이 다형성에 관한 연구
전봉균,이풍연,권무식 성균관대학교 생명과학자원연구소 1994 生命資源科學硏究 Vol.1 No.2
Hemophilia A, an X chromosome-linked bleeding disorder affecting 1 in 5,000 males worldwide, due to the defect of blood coagulation factor Ⅷ. The wide range of clinical severity exhibited by haemophiliacs plus the hight incidence of sporadic cases suggest that hemophilia A be caused heterogeneous mutation of the gene. Restriction fragment length polymorphism(RFLP) is being used for prenatal diagnosis or carrier detection of the genetic disorder, However, the polymorphisms exhibits heterogeniety among races indicating that data from a people may not be applied to others. Two polymorphic sites(Bcl Ⅰ and Hind Ⅲ) of the factor Ⅷ gene were examined in Korean employing polymerase chain reaction(PCR). The PCR product of Bcl Ⅰ restriction was 948 nucleotides(nts) long, while that of Hind Ⅲ was 730 nts long. The former(Bcl Ⅰ) can generate three fragments(90nts, 100nts, and 480nts from 5' to 3' direction) with the polymorphic site, or two fragments(190nts and 480nts from 5' to 3' direction) wihout the polymorphic site. The latter(Hind Ⅲ) can generate three fragments(148nts, 286nts, and 514nts from 5' to 3' direction) with the polymorphic site, or two fragments(434nts and 514nts from 5' to 3' direction) without the polymorphic site. The heterozygote frequency calculated from the allele frequencies(0.754/0.246) of the Bcl Ⅰ (intron 18) polymorphism was 37.1%. The heterozygote frequency calculated from the allele frequencies(0.807/0.193) of the Hind Ⅲ polymorphism(intron 19) was 31.2%. Thus, the two intragenic polymorphisms predicted to be informative was 56.7% in these studies.
배추(Brassica campestris L. var. pekinensis Makino)의 cDNA library 구축 및 상동성 비교
안주미,권오병,전봉균,이풍연,이정민,권무식 성균관대학교 생명과학자원연구소 1995 生命資源科學硏究 Vol.2 No.1
Expressed sequence tag(EST) has a good value to discover a new gene or to study its structure. Some 20 ESTs were generated to obtain new genetic resources of chinese cabbage(Brassian campestris L. var. pekinensis Makino). Poly A+ RNAs were isolated from 10-day-old seedlings grown at 25℃ under the day light. cDNA gene bank was constructed using λ ZAP /cDNA synthesis /Giga Pack Gold Packing kit. About a million clones were able to obtain from the library. All the clones examined so far had insert DNAs. Nucleotide compositions of randomly selected clones were determined by the Sanger mthod. The DNA sequences were compared with those deposited in the GenePept and GenBank database to figure out nucleotide homologies. Two ESTs showed significant similarities to the enlisted sequences. They are chloroplast GADPH subunit of Arabidopsis thaliana and carbonic anhydrase of A. thaliana. The full DNA sequences of the two clones are being determined. The cDNA gene bank constructed. in this experiment will being used to isolate more genes induced by the light in the plant.