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Development the Gene Based Genomic DNA library and STS primer sets in winter cereals
Poo-Reum Oh,Hwa-Young Heo,Byung-Moo Lee,Jong-Jin Hwang,Si-Ju Kim 한국작물학회 2008 한국작물학회 학술발표대회 논문집 Vol.2008 No.10
The purpose of this study is to develop the EEG (Euchromatin Enriched Genomic) DNA library of wheat, barley, rye and oat. Mcr A and Mcr BC system in DH5 alpha bacteria cell line and Kuemkangmil, Olbori, Olhomil and Olgwiri were used for materials in our experiments. EEG colonies have been constructed by using junk DNA exclusion. We analyzed the genetic information of the colonies using blast searches of NCBI and GRAMENE web sites. One hundred eighty-four, 65, 79 and 119 STS primer pairs were developed using sequencing data of selected colonies in Kuemkangmil, Olbori, Olhomil and Olgwiri respectively. Twenty-eight and forty-two percent of designed primer pair showed polymorphism using six endoucleases in Kuemkangmil, Olbori, Olhomil and Olgwiri germplasm respectively. These primers could be useful for specific allele tagging in mapping populations and germplasm and for the study of functional genomics of wheat, barley, rye and oat.
Poo, Ha-Ryoung,Lee, Young-Ik,Todd, Robert F. III,Petty, Howard R. Korean Society for Biochemistry and Molecular Biol 1998 Journal of biochemistry and molecular biology Vol.31 No.1
Recent studies have suggested that integrin (CR3) participates in the signal transduction pathways of certain GPI-anchored phagocytic receptors including $Fc{\gamma}RIIIB$. One consequence of this functional linkage is an inducible association between CR3 and cortical microfilaments that is triggered by $Fc{\gamma}RIIIB$ binding to immobilized immune complexes (IC). That this signaling event requires the co-expression of $Fc{\gamma}RIIIB$ with CR3 was documented by the use of NIH 3T3 transfectants expressing both CR3 and $Fc{\gamma}RIIIB$ (clone 3-23), CR3 alone (clone 3-19), and $Fc{\gamma}RIIIB$ alone (clone 3-15). Pretreatment of 3-23 cells with protein kinase inhibitors such as staurosporine and methyl 2,5-dihydroxycinnamate (MDHC) blocked IC-stimulated CR3 microfilament proximity without affecting the extent to which $Fc{\gamma}RIIIB$ constrains the lateral membrane mobility of a subset of CR3 on the cell surface (as measured in fluorescence recovery after photobleaching experiments). These data support that CR3 and $Fc{\gamma}RIIIB$ molecules are physically and functionally associated and that ligation of FcgRIIIB triggers CR3-dependent signal transduction.
Poo, Haryoung,Lee, Young-Ik,Todd III, Robert F.,Petty, Howard R. The Korea Science and Technology Center 1998 BMB Reports Vol.31 No.1
Recent studies have suggested that integrin (CR3) participates in the signal transduction pathways of certain GPI-anchored phagocytic receptors including FcγRⅢB. One consequence of this functional linkage is an inducible association between CR3 and cortical microfilaments that is triggered by FcγRⅢB binding to immobilized immune complexes (IC). That this signaling event requires the c0-expression of FcγRⅢB with CR3 was documented by the use of NIH 3T3 transfectants expressing both CR3 and FcγRⅢB (clone 3-23), CR3 alone (clone 3-19), and FcγRⅢB alone (clone 3-15). Pretreatment of 3-23 cells with protein kinase inhibitors such as staurosporine and methyl 2,5-dihydroxycinnamate (MDHC) blocked IC-stimulated CR3-microfilament proximity without affecting the extent to which FcγRⅢB constrains the lateral membrane mobility of subset of CR3 on the cell surface (as measured in fluorescence recovery after photobleaching experiments). These data support that CR3 and FcγRⅢB molecules are physically and functionally associated and that ligation of FcgRⅢB triggers CR3-dependent signal transduction.
MULTIPLICATIVE FUNCTIONS COMMUTABLE WITH BINARY QUADRATIC FORMS x<sup>2</sup> ± xy + y<sup>2</sup>
Poo-Sung, Park Korean Mathematical Society 2023 대한수학회보 Vol.60 No.1
If a multiplicative function f is commutable with a quadratic form x<sup>2</sup> + xy + y<sup>2</sup>, i.e., f(x<sup>2</sup> + xy + y<sup>2</sup>) = f(x)<sup>2</sup> + f(x) f(y) + f(y)<sup>2</sup>, then f is the identity function. In other hand, if f is commutable with a quadratic form x<sup>2</sup> - xy + y<sup>2</sup>, then f is one of three kinds of functions: the identity function, the constant function, and an indicator function for ℕ \ pℕ with a prime p.
Advanced Bounding Box Prediction With Multiple Probability Map
Poo-Reum Lee(이푸름),Yoon Kim(김윤) 한국컴퓨터정보학회 2017 韓國컴퓨터情報學會論文誌 Vol.22 No.12
In this paper, we propose a bounding box prediction algorithm using multiple probability maps to improve object detection result of object detector. Although the performance of object detectors has been significantly improved, it is still not perfect due to technical problems and lack of learning data. Therefore, we use the result correction method to obtain more accurate object detection results. In the proposed algorithm, the preprocessed bounding box created as a result of object detection by the object detector is clustered in various form, and a conditional probability is given to each cluster to make multiple probability map. Finally, multiple probability map create new bounding box of object using morphological elements. Experiment results show that the newly predicted bounding box reduces the error in ground truth more than 45% on average compared to the previous bounding box.
Optimal condition for efficient DNA transfer in filamentous cyanobacteria by electroporation
Poo, Ha-Ryoung The Microbiological Society of Korea 1997 The journal of microbiology Vol.35 No.3
Filamentous cyanobacteria are an ecologically important group of bacteria because they are able to provide both organic carbon fixed nitrogen that can support the nutritional requirements for other microorganisms. Because of their prokaryotic nature, they can also be used as potentially powerful model systems for the analysis of oxygenic photosynthesis and nitrogen fixation. Gene transfer is an indispensable procedure for genetic analysis of filamentous cyanobacteria. Electroporation was used to introduce foreign DNA into cyanobacterial cells. In experiments designed to optimize the electroporation technique, the effects of the field strength (amplitude of pulse) and time constant (duration of pulse), DNA concentration and host restriction/modification of DNA on the efficiency of electro-transformation were investigated. The results of this research revelaed that a high voltage pulse of short duration was effective for the electro-transformation of Anabaene sp. M131. The maximal number of transformants was obtained at 6 kV/cm with a pulse duration of 5 msec. The efficiency of electro-transformation was also sensitive to concenetration of DNA; even small amounts of DNA (0.01 .mu.g/ml) were able to gie a large number of transformants (1.0 * 10$\^$3/ cfu/ml).
Poo, Kyung-Min,Son, Eun-Bi,Chang, Jae-Soo,Ren, Xianghao,Choi, Yun-Jung,Chae, Kyu-Jung Elsevier 2018 Journal of environmental management Vol.206 No.-
<P><B>Abstract</B></P> <P>For the purpose of reusing wasted marine macro-algae generated during cultivation, harvesting, processing and selling processes, biochars derived from <I>Saccharina japonica</I> (known as <I>kelp</I>) and <I>Sargassum fusiforme</I> (known as <I>hijikia</I>) were characterized and their removal capacities for Cu, Cd, and Zn in aqueous solution were assessed. Feedstocks, <I>S. japonica, S. fusiforme,</I> and also pinewood sawdust as a control, were pyrolyzed at 250, 400, 500, 600 and 700 °C. In evaluating heavy metal removal capacities, SJB (<I>S. japonica</I> biochar) showed the best performance, with removal efficiencies of more than 98% for the three heavy metals when pyrolyzed at over 400 °C. SFB (<I>S. fusiforme</I> biochar) also showed good potential as an adsorbent, with removal efficiencies for the three heavy metals of more than 86% when pyrolyzed at over 500 °C. On the contrary, the maximum removal efficiencies of PSB (pinewood sawdust biochar) were 81%, 46%, and 47% for Cu, Cd, and Zn, respectively, even at 700 °C, the highest pyrolysis temperature. This demonstrates that marine macro-algae were advantageous in terms of production energy for removing heavy metals even at relatively low pyrolysis temperatures, compared with PSB. The excellent heavy metal adsorption capacities of marine macro-algae biochars were considered due to their higher pH and more oxygen-containing functional groups, although the specific surface areas of SJB and SFB were significantly lower than that of PSB. This research confirmed that the use of marine macro-algae as a heavy metal adsorbent was suitable not only in the removal of heavy metals, but also in terms of resource recycling and energy efficiency.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Marine macro-algae wastes were reused as biochar for the pollutant removal. </LI> <LI> Marine macro-algae biochar (MMAB) showed an excellent heavy metal removal performance. </LI> <LI> MMAB exhibits high pH and abundant oxygen-containing functional groups on the surface. </LI> <LI> The yield and efficiency of MMAB were higher than those of conventional woody biochar. </LI> <LI> MMAB can work efficiently even as pyrolyzed at relatively low temperatures (400–500 °C). </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>