Molecular cloning and characterization of novel human JNK2 (MAPK9) transcript variants that show different stimulation activities on AP-1

( Ping Zhang Wang ),( Ying Xiong ),( Chuan Ma ),( Tai Ping Shi ),( Da Long Ma ) 생화학분자생물학회 (구 한국생화학분자생물학회) 2010 BMB Reports Vol.43 No.11

The c-Jun NH2-terminal kinase (JNK) signaling pathway participates in many physiological functions. In the current study we reported the cloning and characterization of five novel JNK2 transcript variants, which were designated as JNK2 α3, JNK2 α4, JNK2 β3, JNK2 γ1 and JNK2 γ2, respectively. Among them, JNK2 α4 and JNK2 γ2 are potential non-coding RNA because they contain pre-mature stop codons. Both JNK2α3 and JNK2β3 contain an intact kinase domain, and both encode a protein product of 46 kDa, the same as those of JNK2α1 and JNK2β1. JNK2γ1 contains a disrupted kinase domain and it showed a disable function. When over-expressed in mammalian cells, JNK2α3 showed higher activity on AP-1 than that of JNK2β3 and JNK2γ1. Furthermore, JNK2α3 and JNK2β3 showed different levels of substrate phosphorylation, although they both could promote the proliferation of 293T cells. Our results further demonstrate that JNK2 isoforms preferentially target different substrates and may regulate the expression of various target genes. [BMB reports 2010; 43(11): 738-743]

Clinicopathological Features and Immunohistochemical Alterations of Keratinocyte Proliferation, Melanocyte Density, Smooth Muscle Hyperplasia and Nerve Fiber Distribution in Becker`s Nevus

( Ping Sheng ),( Yun-long Cheng ),( Chuan-chuan Cai ),( Wei-jin Guo ),( Ying Zhou ),( Ge Shi ),( Yi-ming Fan ) 대한피부과학회 2016 Annals of Dermatology Vol.28 No.6

Background: Although Becker`s nevus (BN) is a relatively common disease, the systematic studies of clinicopathological and immunohistochemical results are poorly reported. Objective: To investigate the clinicopathological features and immunohistochemical alterations of keratinocyte proliferation, melanocyte density, smooth muscle hyperplasia and nerve fiber distribution in BN. Methods: Clinical and pathological data were collected in 60 newly-diagnosed BN cases. Immunohistochemical stain of Ki-67, Melan-A, keratin 15, smooth muscle actin and protein gene product 9.5 was performed in 21 cases. Results: The median diagnostic and onset age was 17 and 12 years, respectively. Skin lesions usually appeared on the upper trunk and upper limbs. The pathological features included the rete ridge elongation and fusion and basal hyperpigmentation. Epidermal Ki-67, Melan-A and keratin 15 expression and dermal nerve fiber length were significantly higher in lesional and perilesional skin than in normal skin (p<0.05∼0.01), while smooth muscle actin expression was upregulated only in skin lesion (p<0.05). Conclusion: Although the clinical diagnosis of BN is often straightforward, histopathology is helpful to differentiate from other pigmentary disorders. The hyperproliferation of keratinocytes, melanocytes, arrector pili muscle and dermal nerve fibers could be involved in the patho-genesis of BN. (Ann Dermatol 28(6) 697∼703, 2016)

갑골문사료성질지 분석 ( 甲骨文史料性質之 分析 )

장병권 ( Ping Chuan Chang ) 한국중국학회 1966 중국학보 Vol.5 No.1

Impact of Different Wind Representations on Resonant Ocean Near-inertial Motions in the Gulf of Mexico

Chuan-Yuan Hsu,Jongsun Kim,Ping Chang,Steven F. DiMarco 한국해양과학기술원 2022 Ocean science journal Vol.57 No.1

This study investigates the resonated diurnal impact of the surface wind forcing from several atmospheric wind products, including CCMP (Cross-Calibrated Multi-Platform), ERA Interim, NCEP-2 (NCEP-DOE AMIP-II Reanalysis), MERRA (Modern-Era Retrospective analysis for Research and Application), NARR (Northern American Regional Reanalysis) and NAM (North American Mesoscale Forecast System), on the wind-driven near-inertial ocean motions in the northern Gulf of Mexico by numerical simulation, and in comparison, with NDBC buoy surface observed winds. Our analyses show that the near-inertial wind power input, which can affect vertical mixing in the water column, is closely associated with the variability of land-sea breeze by various wind products. In comparison with buoy observations in the northern Gulf of Mexico, the MERRA has the worst land-sea breeze representation, while the NCEP2 and CCMP, which are chosen to represent the coarsest and medium–high horizontal resolution, have the best performance. We further show that by comparison of the buoy data, that the near-inertial wind power input derived from all the wind products is underestimated by 30–50% approximately.

Output Tracking Predictive Control of Networked Systems with Two-channel Random Communication Constraints

Chuan-Dong Bai,Tong Mu,Zhong-Hua Pang,Jian Sun,Guo-Ping Liu 제어·로봇·시스템학회 2023 International Journal of Control, Automation, and Vol.21 No.2

In this paper, the output tracking control problem is investigated for a networked control system with twochannel random network delays and packet dropouts as well as stochastic noise. To actively compensate for these random communication constraints in the feedback and forward channels, a novel networked predictive control (NPC) method is proposed based on the input-output difference equation model, where the two-channel communication constraints are handled separately according to their different features. Furthermore, different from the existing NPC methods based on round-trip time delays, actual control inputs rather than the predicted ones are employed to generate future control commands. Then a delay-independent closed-loop stability condition is obtained, and a condition to guarantee a zero steady-state output tracking error is derived. Also, theoretical analysis shows that the proposed NPC method can achieve the same output tracking performance as the corresponding local control system. Finally, the effectiveness of the proposed method is evaluated by simulation and experimental results.

Comparison of Complications of Peripherally Inserted Central Catheters with Ultrasound Guidance or Conventional Methods in Cancer Patients

Gong, Ping,Huang, Xin-En,Chen, Chuan-Ying,Liu, Jian-Hong,Meng, Ai-Feng,Feng, Ji-Feng Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.5

Objective: To compare the complications of peripherally inserted central catheters (PICC) by a modified Seldinger technique under ultrasound guidance or the conventional (peel-away cannula) technique. Methods: From February to December of 2010, cancer patients who received PICC at the Department of Chemotherapy in Jiangsu Cancer Hospital were recruited into this study, and designated UPICC if their PICC lines were inserted under ultrasound guidance, otherwise CPICC if were performed by peel-away cannula technique. The rates of successful placement, hemorrhage around the insertion area, phlebitis, comfort of the insertion arm, infection and thrombus related to catheterization were analyzed and compared on days 1, 5 and 6 after PICC and thereafter. Results: A total of 180 cancer patients were recruited, 90 in each group. The rates of successful catheter placement between two groups differed with statistical significance (P <0.05), favoring UPICC. More phlebitis and finger swelling were detected in the CPICC group (P <0.05). From day 6 to the date the catheter was removed and thereafter, more venous thrombosis and a higher rate of discomfort of insertion arms were also observed in the CPICC group. Conclusion: Compared with CPICC, UPICC could improve the rate of successful insertion, reduce catheter related complications and increase comfort of the involved arm, thus deserving to be further investigated in randomized clinical studies.

Successful Enrichment of Rarely Found Candidatus Anammoxoglobus propionicus from Leachate Sludge

( Shu Chuan Hsu ),( Yen Chun Lai ),( Ping Heng Hsieh ),( Pun Jen Cheng ),( Suen Shin Wong ),( Chun Hsiung Hung ) 한국미생물 · 생명공학회 2014 Journal of microbiology and biotechnology Vol.24 No.7

Bacteria that mediate the anaerobic oxidation of ammonium (anammox) have been detected in natural ecosystems, as well as various wastewater treatment systems. In this study, sludge from a particular landfill leachate anaerobic treatment system was selected as the incubation seed for anammox microorganism enrichment owing to its possible anammox activity. Transmission electron microscopy observation, denaturing gradient gel electrophoresis analysis, and cloning/sequencing techniques were applied to identify the diversity of anammox microorganisms throughout the incubation. During the early stage of operation, the diversity of anammox microorganisms was similar to the original complex microbes in the seed sludge. However, as incubation time increased, the anammox microorganism diversity within the system that was originally dominated by Candidatus (Ca.) Brocadia sp. was replaced by Ca. Anammoxoglobus propionicus. The domination of Ca. Anammoxoglobus propionicus produced a stable removal of ammonia (70 mg-N/l) and nitrite (90 mg-N/l), and the total nitrogen removal efficiency was maintained at nearly 95%. The fluorescence in situ hybridization results showed that Ca. Anammoxoglobus propionicus was successfully enriched from 1.8 ± 0.6% initially to 65 ± 5% after 481 days of operation. Therefore, the present results demonstrated the feasibility of enriching Ca. Anammoxoglobus propionicus from leachate sludge, even though the original cell count was extremely low. Application of this seldom found anammox organism could offer an alternative to current ammonia-nitrogen treatment.

Poly(trimethylene terephthalate) Fiber Melt-Spinning: Material Parameters and Computer Simulation

Zhang, Chuan Xiong,Wang, Hua Ping,Wang, Chao Sheng The Korean Fiber Society 2007 Fibers and polymers Vol.8 No.3

In this work, the method, in principle ofthe box complex algorithm was adopted to obtain stress-induced crystallization coefficient C and the strain-optical coefficient $A_{op}$ with the value of 295 and $1.5{\times}10^{-9}$, respectively, and some parameters $A_1=0.27$, $A_2=5.06$, a= 3.5, b=1.8 relative to the elongational viscosity of poly(trimethylene terephthalate)(PTT) fiber. The vitrification distance as a function of the take-up velocity and mass throughput was also gotten. The effects of spinning conditions on filament temperature, velocity gradient, spinning tension, birefringence and crystallinity, and effect of viscoelasticity on take-up velocity had been discussed.

Effect of rapid thermal oxidation on structure and photoelectronic properties of silicon oxide in monocrystalline silicon solar cells

Chung Ping Liu,Ming Wei Chang,Chuan Lung Chuang 한국물리학회 2014 Current Applied Physics Vol.14 No.5

This paper concerns the topic of surface passivation properties of rapid thermal oxidation on p-type monocrystalline silicon wafer for use in screen-printed silicon solar cells. It shows that inline thermal oxidation is a very promising alternative to the use of conventional batch type quartz tube furnaces for the surface passivation of industrial phosphorus-diffused emitters. Five minutes was the most favorable holding time for the rapid thermal oxidation growth of the solar cell sample, in which the average carrier lifetime was increased 19.4 ms. The Fourier transform infrared spectrum of the rapid thermal oxidation sample, whose structure was Al/Al-BSF/p-type Si/n-type SiP/SiO2/SiNx/Ag solar cell with an active area of 15.6 cm2, contained an absorption peak at 1085 cm1, which was associated with the SieO bonds in silicon oxide. The lowest average reflectance of this sample is 0.87%. Furthermore, for this sample, its average of internal quantum efficiency and conversion efficiency are respectively increased by 8% and 0.23%, compared with the sample without rapid thermal oxidation processing.

Green and sustainable applications of energetic plasmon-activated water

Chih-Ping Yang,Shih-Hao Yu,Yu-Chuan Liu 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.111 No.-

This review describes the use of bulk deionized water (DIW) treatment to become plasmon-activatedwater (PAW) with reduced hydrogen bonds (HBs). PAW with special properties can be used in innovativeapplications and development of environmental protection science and sustainable energy management. PAW is a natural water solvent, but PAW subverts the public’s idea that hydrophobic solutes are difficultto dissolve in water. PAW can dissolve more-hydrophobic solutes than ordinary water, and can also interactwith water-soluble alcohol molecules to form stronger HBs. Moreover, in the application of certainreduction reactions, no chemical reducing agent is needed, and the catalytic reduction effect can beachieved using natural PAW. A characteristic application of PAW can also be extended to seawater desalination. Due to weak HBs among PAW molecules, high water evaporation rates can be produced, and ahigher chemical potential can also be applied to the innovative development of solar energy storage. In electrochemical applications, PAW itself can promote water oxidation, oxygen reduction reactions,hydrogen and oxygen evolution reactions, and the efficient capacitor production of H2O2. Furthermore,PAW can delay the melting of sea ice in the habitat of polar bears, so it may be applied to the issue ofsaving polar bears.