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BDNF, ERK 및 CREB 경로를 통한 포공영 추출물의 항우울 효과
구필성 ( Pil Sung Gu ),이지혜 ( Ji Hye Lee ),최윤희 ( Yun Hee Choi ),정지욱 ( Ji Wook Jung ) 대한본초학회 2015 大韓本草學會誌 Vol.30 No.3
Objectives : Taraxacum platycarpum H. Dahlstedt has been reported to have several biological properties such as skin hydration and antiinflammation. The purpose of this study was to examine the antidepressive effects of water extract of T. platycarpum (WTP) on an animal model of depression. Methods : In the present study, normal ICR mice (4 weeks) were used, and orally administered with WTP (25, 50 and 100 mg/kg). Depression-like behavior was monitored the forced swimming test (FST) and tail suspension test (TST) in mice. The locomotor activity was evaluated to eliminate the false-positive activity in the open field test (OFT). Fluoxetine, the selective serotonin reuptake inhibitor, as a positive control was intraperitoneally administered at a dose of 15 mg/kg at 30 min before starting the behavioral test. Moreover, we evaluated the effects of WTP on the expression of brain-derived neurotrophic factor (BDNF) and the extracellular signal-regulated kinase (ERK)/ cyclic AMP response-element binding protein (CREB) signaling pathway in the hippocampus using Western blot. Results : The administration of WTP (50 and 100 mg/kg) significantly (P < 0.05, respectively) reduced the immobility time during FST and TST without accompanying changes in locomotor activity by OFT. Furthermore, WTP at dose of 100 mg/kg increased the BDNF expression and the phosphorylation of ERK and CREB in the hippocampus region. Conclusions : These results suggest that WTP has a useful anti-depressant effect through the regulation of BDNF/ERK/CREB signaling pathway.
Park, Pil-Gu,Cho, Min-Hee,Rhie, Gi-eun,Jeong, Haeseul,Youn, Hyewon,Hong, Kee-Jong The Korean Vaccine Society 2012 Clinical and Experimental Vaccine Research Vol.1 No.1
<P><B>Purpose</B></P><P>In vaccine efficacy evaluation, visualization of pathogens in whole organism at each time point would be able to reduce the consuming animals and provide the <I>in vivo</I> information within consistent background with identical organism.</P><P><B>Materials and Methods</B></P><P>Using IVIS spectrum whole live-animal imaging system, fluorescent intensity was optimized and visualized proportionately by concentrating <I>Escherichia coli</I> MC1061 strain which expresses GFP (<I>E. coli</I>-GFP) in BALB/C mice after injection.</P><P><B>Results</B></P><P>Local distribution of disseminated <I>E. coli</I>-GFP was traced in each organ by fluorescence. Detached organ showed more obvious fluorescent signal, and intestine showed strongest fluorescent signal.</P><P><B>Conclusion</B></P><P>This <I>in vivo</I> imaging method using GFP-tagged pathogen strain suggest quantified infected pathogens by fluorescence intensity in whole animals can provide the information about the localization and distribution after infection.</P>
Current development of therapeutic vaccines for the treatment of chronic infectious diseases
Park Pil-Gu,Fatima Munazza,An Timothy,Moon Ye-Eun,Woo Seungkyun,Youn Hyewon,Hong Kee-Jong 대한백신학회 2024 Clinical and Experimental Vaccine Research Vol.13 No.1
Chronic infectious diseases refer to diseases that require a long period of time from onset to cure or death, the use of therapeutic vaccines has recently emerged to eradicate diseases. Currently, clinical research is underway to develop therapeutic vaccines for chronic infectious diseases based on various vaccine formulations, and the recent success of the messenger RNA vaccine platform and efforts to apply it to therapeutic vaccines are having a positive impact on conquering chronic infectious diseases. However, since research on the development of therapeutic vaccines is still relatively lacking compared to prophylactic vaccines, there is a need to focus more on the development of therapeutic vaccines to overcome threats to human health caused by chronic infectious diseases. In order to accelerate the development of therapeutic vaccines for chronic infectious diseases in the future, it is necessary to establish a clear concept of therapeutic vaccines suitable for the characteristics of each chronic infectious disease, as well as standardize vaccine effectiveness evaluation methods, secure standards/reference materials, and simplify the vaccine approval procedure.