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Development of a fast transient simulator for gas-liquid two-phase flow in pipes
Choi, J.,Pereyra, E.,Sarica, C.,Lee, H.,Jang, I.S.,Kang, J. Elsevier 2013 Journal of petroleum science & engineering Vol.102 No.-
The objective of this study is to develop a simplified transient model and a simulator for gas-liquid two-phase flow in pipelines. The reliable predictions of liquid holdup and pressure drop are essential for pipeline design in oil and gas industry. In this study, the drift-flux approach is utilized to calculate liquid holdups. A modification of the power law correlation presented by Al-sarkhi and Sarica (2009) is suggested for pressure drop calculation. The proposed approach and correlation are continuous and flow pattern independent. Additionally, the developed model is simple and presents an easy tuning capability with either experimental data or synthetic data coming from steady state simulators. The simplicity of the model allows quick implementation yielding in a faster simulator as compared to available commercial software. The developed simulator is tested with Vigneron et al. (1995) experimental data, which include two transient conditions; liquid flow rate changes and gas flow rate changes. The results of simulation are compared with OLGA (OiL and GAs simulator supplied by SPT group) simulations and show fair agreement in terms of liquid holdups and pressures.
Elisa Crespi,Ana M. Pereyra,Tomás Puigdevall,María V. Rumi,María F. Testorelli,Nicolás Caggiano,Lucía Gulone,Marta Mollerach,Elida R. Gentilini,Mariela E. Srednik 대한수의학회 2022 Journal of Veterinary Science Vol.23 No.6
Background: Staphylococcus aureus and Streptococcus agalactiae are the main cause of clinical mastitis in dairy cattle in Argentina, whereas coagulase-negative staphylococci (CNS) and environmental streptococci are the main cause of subclinical mastitis. Bacteria isolated from infected animals show increasing antimicrobial resistance. Objectives: This study aims to determine the antimicrobial resistance of staphylococci and streptococci isolated from milk with mastitis, and to genotypically characterize the methicillin-resistant (MR) staphylococci. Methods: Isolation was performed on blood agar and identification was based on biochemical reactions. Antimicrobial susceptibility was according to the Clinical and Laboratory Standards Institute guidelines. The antimicrobial resistance genes, SCCmec type and spa type were detected by the polymerase chain reaction method. Results: We isolated a total of 185 staphylococci and 28 streptococci from 148 milk samples. Among the staphylococcal isolates, 154 were identified as CNS and 31 as S. aureus. Among the 154 CNS, 24.6% (n = 38) were resistant to penicillin, 14.9% (n = 23) to erythromycin, 17.5% (n = 27) to clindamycin, 6.5% (n = 10) to cefoxitin and oxacillin. Among the S. aureus isolates, 16.1% (n = 5) were resistant to penicillin, 3.2% (n = 1) to cefoxitin and oxacillin (MRSA). Six MR isolates (5 CNS and 1 MRSA) were positive to the mecA gene, and presented the SCCmec IVa. The MRSA strain presented the sequence type 83 and the spa type 002. Among the 28 streptococcal isolates, 14.3% (n = 4) were resistant to penicillin, 10.7% (n = 3) to erythromycin and 14.3% (n = 4) to clindamycin. Conclusions: The present findings of this study indicate a development of antimicrobial resistance in main bacteria isolated from cows with mastitis in Argentina.
Lee, H.,Al-Sarkhi, A.,Pereyra, E.,Sarica, C.,Park, C.,Kang, J.,Choi, J. Elsevier 2013 Journal of petroleum science & engineering Vol.108 No.-
A new hydrodynamics model is proposed using minimum energy dissipation concept for gas-liquid stratified flow in horizontal pipes. It determines the liquid holdup and the pressure drop in gas-liquid stratified flow without the need of interfacial friction factor closure relationship. Model predictions show fair agreement when compared with experimental data and existing mechanistic models.
Antonio Montes,Chandrasekar Chinnarasu,Clara Pereyra,Lourdes Casas,María Teresa Fernández-Ponce,Casimiro Mantell,Sangma Pattabhi,Enrique Martínez de la Ossa 한국화학공학회 2016 Korean Journal of Chemical Engineering Vol.33 No.2
Various extracts from olive leaves have been precipitated by a supercritical antisolvent (SAS) process to evaluate the possibility of producing polyphenol fine particles with controlled size and size distribution. Olive leaves were initially extracted with subcritical fluids using mixtures of CO2+ethanol at 10% and 50%, by pressurized liquid extraction (PLE) with water, ethanol and a hydroalcoholic mixture (50 : 50) (v/v), and also by conventional ethanol extraction (CE). PLE gave the extract with the highest yield and the best antioxidant activity. SAS precipitation was unsuccessful for the extracts obtained with pressurized water and with the hydroalcoholic mixture (50 : 50) (v/v). The SAS precipitates with the smallest particle sizes were produced from extracts obtained with subcritical fluids. The SAS precipitates obtained after the conventional ethanol extraction of olive leaves showed the best antioxidant activity.
Analysis of the transcripts encoding for antigenic proteins of Bovine Gammaherpesvirus 4
Florencia Romeo,Maximiliano J. Spetter,Pedro Moran,Susana Pereyra,Anselmo Odeon,Sandra E. Perez,Andrea E. Verna 대한수의학회 2020 Journal of Veterinary Science Vol.21 No.1
The major glycoproteins of bovine gammaherpesvirus 4 (BoHV-4) are gB, gH, gM, gL, and gp180 with gB, gH, and gp180 being the most glycosylated. These glycoproteins participate in cell binding while some act as neutralization targets. Glycosylation of these envelope proteins may be involved in virion protection against neutralization by antibodies. In infected cattle, BoHV-4 induces an immune response characterized by low neutralizing antibody levels or an absence of such antibodies. Therefore, virus seroneutralization in vitro cannot always be easily demonstrated. The aim of this study was to evaluate the neutralizing capacity of 2 Argentine BoHV-4 strains and to associate those findings with the gene expression profiles of the major envelope glycoproteins. Expression of genes coding for the envelope glycoproteins occurred earlier in cells infected with isolate 10/154 than in cells infected with strain 07/435, demonstrating a distinct difference between the strains. Differences in serological response can be attributed to differences in the expression of antigenic proteins or to post-translational modifications that mask neutralizing epitopes. Strain 07/435 induced significantly high titers of neutralizing antibodies in several animal species in addition to bovines. The most relevant serological differences were observed in adult animals. This is the first comprehensive analysis of the expression kinetics of genes coding for BoHV-4 glycoproteins in 2 Argentine strains (genotypes 1 and 2). The results further elucidate the BoHV-4 life cycle and may also help determine the genetic variability of the strains circulating in Argentina.