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Padmanaban, G.,Park, H.,Choi, J.S.,Cho, Y.W.,Kang, W.C.,Moon, C.I.,Kim, I.S.,Lee, B.H. Elsevier Science Publishers 2014 Journal of biotechnology Vol.187 No.-
Biopanning of phage displayed-peptide library was performed against myoglobin, a marker for the early assessment of acute myocardial infarction (AMI), to identify peptides that selectively bind to myoglobin. Using myoglobin-conjugated magnetic beads, phages that bound to myoglobin were collected and amplified for the next round of screening. A 148-fold enrichment of phage titer was observed after five rounds of screening relative to the first round. After phage binding ELISA, three phage clones were selected (3R1, 3R7 and 3R10) and the inserted peptides were chemically synthesized. The analysis of binding affinity showed that the 3R7 (CPSTLGASC) peptide had higher binding affinity (K<SUB>d</SUB>=57nM) than did the 3R1 (CNLSSSWIC) and 3R10 (CVPRLSAPC) peptide (K<SUB>d</SUB>=125nM and 293nM, respectively). Cross binding activity to other proteins, such as bovine serum albumin, troponin I, and creatine kinase-MB, was minimal. In a peptide-antibody sandwich ELISA, the selected peptides efficiently captured myoglobin. Moreover, the concentrations of myoglobin in serum samples measured by a peptide-peptide sandwich assay were comparable to those measured by a commercial antibody-based kit. These results indicate that the identified peptides can be used for the detection of myoglobin and may be a cost effective alternative to antibodies.
인삼의 Flowering Locus T의 동종 유전자 탐구 및 개화시기 조절
Padmanaban Mohanan,Davajargal Myagmarav,김유진,양덕춘 한국약용작물학회 2018 한국약용작물학술대회 발표집 Vol.2018 No.10
Background : Panax ginseng Meyer, the king of Oriental medicine is a geophytic perennial shrub. P. ginseng possess long juvenile period which spans for at least 3-years before it could attain reproductive competence. To explore the significance of flowering time genes we isolated, characterized the Flowering Locus T (FT) gene and named as PgFT1 and PgFT2 to understand their role in ginseng floral transition. Methods and Results : By using extensive cloning and bioinformatics analysis we identified two FT homologs from ginseng a. The deduced PgFT sequences has a considerable homology to established FT homologs with the essential amino acids of a florigen function conserved. The tissue specific expression profiling by Real-time qPCR from the juvenile (2-years-old) and adult (4-year-old) ginseng plants have shown a spatial and temporal expression pattern, furthermore PgFT transcripts exhibited photoperiod dependent oscillation as well. Besides, PgFT1 and PgFT2 have shown an age specific expression pattern, in particular the transcripts were concentrated to the roots of P. ginseng. The ectopic expression of PgFT homologs in Arabidopsis thaliana induce precocious flowering by altering several floral pathway integrators, in addition PgFT1 transgenic Arabidopsis seedlings had an increased root length while, the influence of PgFT2 in transgenic seedlings apart from flowering is as yet to be revealed. Conclusion : Based on our results, we alleged the spatial and temporal expression of PgFT homologs could be significant in the flowering time transition of ginseng and their age specific expression pattern suggests the roots of ginseng are influential in determining the reproductive competency. On top of it, results from transgenic studies in Arabidopsis claimed that PgFT1 might involve in the underground organ development. Albeit, their molecular regulation, developmental role and the manipulation of PgFTs in ginseng flowering warranties further investigation.
Development of SNP Marker to Authenticate Different Species of Sea Buckthorn by Multiplex ARMS-PCR
Padmanaban Mohanan(모하난 파드마나반),Jong Chan Ahn(안종찬),Hong Tao Wang(왕홍도),Gokulanathan Anandapadmanaban(아난다파드마나반 고클라나탄),Hyun A Lee(이현아),Yu Jin Kim(김유진),Doek Chun Yang(양덕춘) 한국약용작물학회 2017 한국약용작물학회 학술대회논문집 Vol.2017 No.2
Padmanaban Mohanan,허준,김선오,나주련,김유진,이현아,김민경,양덕천 한국식물생명공학회 2019 Plant biotechnology reports Vol.13 No.3
The traditional oriental medicine bokbunja, prepared from immature berries of Rubus coreanus is used as an anti-oxidant, diuretic, and cure for impotence. The bokbunja wine made from fermented fruits of bokbunja has been used as a functional food as well. However, the usage of bokbunja has been problematic over the years due to the abundance of mistakenly identified berries such as Rubus chingii, Rubus crataegifolius, and Rubus occidentalis. Thus, here we developed a method for the molecular differentiation of Rubus species as well as the authentication bokbunja from other Rubus species. We screened several sequences from the chloroplast DNA of these species and found that the rpl16 region was polymorphic for R. coreanus and R. occidentalis, while the trnG–trnS intergenic spacer region was polymorphic for R. chingii and R. crataegifolius. Species-specific primers were designed and a multiplex PCR was performed by combining the markers at the rpl16 and trnG–trnS regions. Amplicons of 686 bp for R. coreanus and 478 bp for R. occidentalis were produced by the primers 5′ Rcor or 5′ Rocci, respectively, with 3′ rpl16; whereas, amplicons of 389 bp for Rubus crataegifolius and 180 bp for R. chingii were produced by 5′ Rcra or 5′ trnG–trnS, respectively, and 3′ Rcra/Rchi. The deduced molecular markers were utilized to authenticate the bokbunja products and demonstrated that the majority of bokbunja samples from the markets were adulterant berries. Hence, our results indicate that the produced molecular markers can serve as an effective tool to authenticate bokbunja.
Padmanaban, Sudakar,Kim, Minog,Yoon, Sungho THE KOREAN SOCIETY OF INDUSTRIAL AND ENGINEERING 2019 JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY -S Vol.71 No.-
<P><B>Abstract</B></P> <P>Engineering the surface of Metal-organic frameworks (MOFs) will afford to advantageously improve their performances in catalysis. A particular example of industrially applied MOF-based catalysis is the zinc glutarate (ZnGA) catalyzed copolymerization of CO<SUB>2</SUB> with an epoxide. Herein, we demonstrate how the modification of the surface of ZnGA aids to enhance its productivity in fixing CO<SUB>2</SUB> into poly(alkylene carbonates), and deliver an industrially viable procedure to prepare a nanosized, surface-etched ZnGA by systematically controlling the reaction conditions and by using a mild-HCl solution. This surface modified-ZnGA exhibits ∼83% increased productivity than the standard-ZnGA and thus could be readily applicable in industries.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Molecular signaling of ginsenosides Rb1, Rg1, and Rg3 and their mode of actions
Mohanan, Padmanaban,Subramaniyam, Sathiyamoorthy,Mathiyalagan, Ramya,Yang, Deok-Chun The Korean Society of Ginseng 2018 Journal of Ginseng Research Vol.42 No.2
Ginseng has gained its popularity as an adaptogen since ancient days because of its triterpenoid saponins, known as ginsenosides. These triterpenoid saponins are unique and classified as protopanaxatriol and protopanaxadiol saponins based on their glycosylation patterns. They play many protective roles in humans and are under intense research as various groups continue to study their efficacy at the molecular level in various disorders. Ginsenosides Rb1 and Rg1 are the most abundant ginsenosides present in ginseng roots, and they confer the pharmacological properties of the plant, whereas ginsenoside Rg3 is abundantly present in Korean Red Ginseng preparation, which is highly known for its anticancer effects. These ginsenosides have a unique mode of action in modulating various signaling cascades and networks in different tissues. Their effect depends on the bioavailability and the physiological status of the cell. Mostly they amplify the response by stimulating phosphotidylinositol-4,5-bisphosphate 3-kinase/protein kinase B pathway, caspase-3/caspase-9-mediated apoptotic pathway, adenosine monophosphate-activated protein kinase, and nuclear factor kappa-light-chain-enhancer of activated B cells signaling. Furthermore, they trigger receptors such as estrogen receptor, glucocorticoid receptor, and N-methyl-$\text\tiny{D}$-aspartate receptor. This review critically evaluates the signaling pathways attenuated by ginsenosides Rb1, Rg1, and Rg3 in various tissues with emphasis on cancer, diabetes, cardiovascular diseases, and neurodegenerative disorders.
Sudakar Padmanaban,이윤호,윤성호 한국공업화학회 2021 Journal of Industrial and Engineering Chemistry Vol.94 No.-
Selective hydrogenation of the carbonyl functional group of α,β-unsaturated carbonyl compounds affordsindustrially important allylic alcohols. However, achieving the selective reduction of the carbonyl groupin the presence of the activated olefinic group is challenging. Therefore, the development of a highlychemoselective, efficient, and recyclable catalyst for this transformation is greatly desirable from theindustrial and environmental viewpoints. In this study, a Ru-immobilized bisphosphine-based porousorganic polymer (Ru@PP-POP) was used as an efficient heterogeneous catalyst for chemoselectivehydrogenation of cinnamaldehyde (CAL) to cinnamyl alcohol with high chemoselectivity (98%) andexcellent recyclability. To the best of our knowledge, the catalyst, Ru@PP-POP showed a high turnovernumber (970) and a high turnover frequency (240 h 1) which is the best activity obtained using aphosphine based heterogeneous Ru-catalyst in this transformation.
Sudakar Padmanaban,김민옥,윤성호 한국공업화학회 2019 Journal of Industrial and Engineering Chemistry Vol.71 No.-
Engineering the surface of Metal-organic frameworks (MOFs) will afford to advantageously improve theirperformances in catalysis. A particular example of industrially applied MOF-based catalysis is the zincglutarate (ZnGA) catalyzed copolymerization of CO2 with an epoxide. Herein, we demonstrate how themodification of the surface of ZnGA aids to enhance its productivity infixing CO2 into poly(alkylenecarbonates), and deliver an industrially viable procedure to prepare a nanosized, surface-etched ZnGA bysystematically controlling the reaction conditions and by using a mild-HCl solution. This surfacemodified-ZnGA exhibits83% increased productivity than the standard-ZnGA and thus could be readilyapplicable in industries.