Crashworthy and Performance-cost Characteristics of Aluminum-CFRP Hybrid Tubes under Quasi-static Axial Loading

Peilong Shi,Qiang Yu,Rong Huang,Xuan Zhao,Guohua Zhu 한국섬유공학회 2019 Fibers and polymers Vol.20 No.2

Metal/composite hybrid thin-walled structures combine the low cost of metallic materials and the high strength-toweight ratio of composites and thus have the potential to be utilized as cost-effective energy absorbers for vehicle applications. This study aimed to examine the crushing behaviors and performance-to-cost ratio of aluminum/carbon fiberreinforced plastic (CFRP) hybrid tubes under quasi-static axial loading. First, a single aluminum tube, a single CFRP tube and an aluminum/CFRP hybrid tube were tested to validate numerical models. The experimental results showed that the total energy absorption (EA) of the aluminum/CFRP hybrid tube was 32.46 % higher than the sum of that of the individual parts, and the special energy absorption (SEA) of the hybrid tube was improved by 105.26 % compared with that of the single aluminum tube. Then, the effects of the orientation angles ([±15 o]n, [±45 o]n, [±75 o]n, [±90 o]n, [90 o/0 o]n, n=2, 4, 6) and thicknesses of the CFRP tube wall (4-ply, 8-ply, 12-ply) on the crashworthiness of the hybrid tube were studied through validated numerical models. The numerical results showed that as the orientation angle increased, SEA, EA and the mean crushing force (Fmean) decreased first and then increased; in addition, the hybrid tubes with orientation angles of [±45 o]n and [90 o/0 o]n (n=2, 4, 6) consistently exhibited the worst and best crashworthiness, respectively. Furthermore, the SEA, EA, and Fmean of the hybrid tube increased with increasing thickness of the CFRP tube wall. Finally, the performance-to-cost ratio (SEA/cost) of the hybrid tube was analyzed, and the results show that aluminum/CFRP hybrid tubes with a smaller wall thickness of the CFRP tube exhibits superior potential in terms of both cost and performance for automotive applications.

Accurate computational design of multipass transmembrane proteins

Lu, Peilong,Min, Duyoung,DiMaio, Frank,Wei, Kathy Y.,Vahey, Michael D.,Boyken, Scott E.,Chen, Zibo,Fallas, Jorge A.,Ueda, George,Sheffler, William,Mulligan, Vikram Khipple,Xu, Wenqing,Bowie, James U. American Association for the Advancement of Scienc 2018 Science Vol.359 No.6379

<P><B>Membrane protein oligomers by design</B></P><P>In recent years, soluble protein design has achieved successes such as artificial enzymes and large protein cages. Membrane proteins present a considerable design challenge, but here too there have been advances, including the design of a zinc-transporting tetramer. Lu <I>et al.</I> report the design of stable transmembrane monomers, homodimers, trimers, and tetramers with up to eight membrane-spanning regions in an oligomer. The designed proteins adopted the target oligomerization state and localized to the predicted cellular membranes, and crystal structures of the designed dimer and tetramer reflected the design models.</P><P><I>Science</I>, this issue p. 1042</P><P>The computational design of transmembrane proteins with more than one membrane-spanning region remains a major challenge. We report the design of transmembrane monomers, homodimers, trimers, and tetramers with 76 to 215 residue subunits containing two to four membrane-spanning regions and up to 860 total residues that adopt the target oligomerization state in detergent solution. The designed proteins localize to the plasma membrane in bacteria and in mammalian cells, and magnetic tweezer unfolding experiments in the membrane indicate that they are very stable. Crystal structures of the designed dimer and tetramer—a rocket-shaped structure with a wide cytoplasmic base that funnels into eight transmembrane helices—are very close to the design models. Our results pave the way for the design of multispan membrane proteins with new functions.</P>

ELECTROCHEMICAL Co 3 O 4 NANOPOROUS THIN FILMS SENSOR FOR HYDROGEN PEROXIDE DETECTION

GUANG SHENG CAO,RUILIN WANG,PEILONG WANG,XIN LI,YUE WANG,JUNPING LI,GUILONG WANG 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2014 NANO Vol.9 No.4

The nanoporous Co 3 O 4 thin ¯lms were prepared on indium tin oxide (ITO) glasses by anelectrodeposition method. The surface morphology and composition of the nanoporous Co 3 O 4¯lms were characterized by scanning electron microscopy (SEM), energy-dispersive X-rayanalysis (EDS) and X-ray photoelectron spectroscopy (XPS). The results show that the as-deposited nanoporous Co 3 O 4 ¯lm is constructed by many interconnected nano°akes withthickness of about 40 nm. The cyclic voltammetry (CV) measurement indicates that thenanoporous Co 3 O 4 ¯lms exhibit remarkable electrocatalytic activities for the hydrogen peroxide(H 2 O 2 ) reduction which shows that it is a good candidate to be employed as electrode materialsfor electrochemical sensing of H 2 O 2 . Further analysis indicated that the detection sensitivityof the sensor was 1.357 mA mM? 1 cm? 2 and the detection limit was estimated to be about0.2 mM.

Colorimetric Detection of Cr3+ in Aqueous Solution Based on Confunctionalized Silver Nanoparticles Modified with 4-Nitrobenzenethiol and 4-Mercaptobenzoic Acid

Zhikun Zhang,Ying Zhou,Jing-Kui Yang,Peilong Wang,Xiaoou Su,Hong Zhao,Yujian He,Zhiqin Cao,Maoqiang Luo 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2015 NANO Vol.10 No.7

A new method has been proposed to realize the visual detection of Cr3+ using 4-nitrobenzenethiol (4-NBT) and 4-mercaptobenzoic acid (4-MBA) modified silver nanoparticles (AgNPs). The presence of Cr3+ induces the aggregation of AgNPs through cooperative metal–ligand interaction, resulting in a color change from bright yellow to purple. Consequently, Cr3+ could be monitored by colorimetric response of AgNPs by a UV-Vis spectrophotometer or even naked eyes. We firstly used ethylene diamine tetraacetic acid (EDTA) as a masking agent to selectively detect Cr3+, and other metal ions have little influence on the Cr3+–AgNPs system. The cofunctionalized AgNPs exhibited a highly sensitive detection limit of Cr3+, which is as low as 5 x 10-9 mol L-1, and the absorbance ratio (A600nm /A387nm) is linear with the concentration of Cr3+ ranging from 5 x 10-9 mol L-1 to 2 x 10-6 mol L-1 with a coefficient of 0.993. Particularly, the sensor has been further evaluated to monitor the concentration of Cr3+ in drinking water, the recovery was in good agreement with those obtained by ICP-MS, indicating that this proposed method is successfully applied in real samples.

Three-terminal nonvolatile memory photodetectors based on rationally engineered heterostructured tin zinc oxide nanowires

Zhang WenXin,Li Hao,Cong Haofei,Zhou Ruifu,Qin Yuanbin,Xu Peilong,Liu Xuhai,Wang Fengyun 한국물리학회 2023 Current Applied Physics Vol.48 No.-

One-dimensional wide band-gap metal oxide semiconductors demonstrate enormous potential in high-performance ultraviolet photodetectors. Here, via tuned zinc oxide (ZnO) concentration ratio and heterojunction structure as well as decent VTH value, we electrospun tin zinc oxide (SnZnOX) nanowires for photoelectronic conversion function. The constructed optimized photodetectors based on Sn8Zn2OX nanowires can exhibit a remarkable detectivity of 1.77 × 1016 Jones, excellent responsivity of 2.23 × 103 A/W and relative high sensitivity over 104. In addition to the excellent photodetection performance, Sn8Zn2OX nanowire devices exhibit nonvolatile memory effect, which can maintain the light-induced low resistance state at 5 V without light. Moreover, a voltage of 30 V was applied to Sn8Zn2OX nanowires devices to realize light-writing state switching to bias-erasing state, and the device could come back to their initial high resistance state. These results indicate SnZnOX nanowires devices possess a tremendous potential in the circuit design of the artificial visual system.

Severe choline deficiency induces alternative splicing aberrance in optimized duck primary hepatocyte cultures

Zhao Lulu,Cai Hongying,Wu Yongbao,Tian Changfu,Wen Zhiguo,Yang Peilong 아세아·태평양축산학회 2022 Animal Bioscience Vol.35 No.11

Objective: Choline deficiency, one main trigger for nonalcoholic fatty liver disease (NAFLD), is closely related to lipid metabolism disorder. Previous study in a choline-deficient model has largely focused on gene expression rather than gene structure, especially sparse are studies regarding to alternative splicing (AS). In modern life science research, primary hepatocytes culture technology facilitates such studies, which can accurately imitate liver activity in vitro and show unique superiority. Whereas limitations to traditional hepatocytes culture technology exist in terms of efficiency and operability. This study pursued an optimization culture method for duck primary hepatocytes to explore AS in choline-deficient model. Methods: We performed an optimization culture method for duck primary hepatocytes with multi-step digestion procedure from Pekin duck embryos. Subsequently a NAFLD model was constructed with choline-free medium. RNA-seq and further analysis by rMATS were performed to identify AS events alterations in choline-deficency duck primary hepatocytes. Results: The results showed E13 (embryonic day 13) to E15 is suitable to obtain hepatocytes, and the viability reached over 95% by trypan blue exclusion assay. Primary hepatocyte retained their biological function as well identified by Periodic Acid-Schiff staining method and Glucose-6-phosphate dehydrogenase activity assay, respectively. Meanwhile, genes of alb and afp and specific protein of albumin were detected to verify cultured hepatocytes. Immunofluorescence was used to evaluate purity of hepatocytes, presenting up to 90%. On this base, choline-deficient model was constructed and displayed significantly increase of intracellular triglyceride and cholesterol as reported previously. Intriguingly, our data suggested that AS events in choline-deficient model were implicated in pivotal biological processes as an aberrant transcriptional regulator, of which 16 genes were involved in lipid metabolism and highly enriched in glycerophospholipid metabolism. Conclusion: An effective and rapid protocol for obtaining duck primary hepatocytes was established, by which our findings manifested choline deficiency could induce the accumulation of lipid and result in aberrant AS events in hepatocytes, providing a novel insight into various AS in the metabolism role of choline. Objective: Choline deficiency, one main trigger for nonalcoholic fatty liver disease (NAFLD), is closely related to lipid metabolism disorder. Previous study in a choline-deficient model has largely focused on gene expression rather than gene structure, especially sparse are studies regarding to alternative splicing (AS). In modern life science research, primary hepatocytes culture technology facilitates such studies, which can accurately imitate liver activity <i>in vitro</i> and show unique superiority. Whereas limitations to traditional hepatocytes culture technology exist in terms of efficiency and operability. This study pursued an optimization culture method for duck primary hepatocytes to explore AS in choline-deficient model.Methods: We performed an optimization culture method for duck primary hepatocytes with multi-step digestion procedure from Pekin duck embryos. Subsequently a NAFLD model was constructed with choline-free medium. RNA-seq and further analysis by rMATS were performed to identify AS events alterations in choline-deficency duck primary hepatocytes.Results: The results showed E13 (embryonic day 13) to E15 is suitable to obtain hepatocytes, and the viability reached over 95% by trypan blue exclusion assay. Primary hepatocyte retained their biological function as well identified by Periodic Acid-Schiff staining method and Glucose-6-phosphate dehydrogenase activity assay, respectively. Meanwhile, genes of <i>alb</i> and <i>afp</i> and specific protein of albumin were detected to verify cultured hepatocytes. Immunofluorescence was used to evaluate purity of hepatocytes, presenting up to 90%. On this base, choline-deficient model was constructed and displayed significantly increase of intracellular triglyceride and cholesterol as reported previously. Intriguingly, our data suggested that AS events in choline-deficient model were implicated in pivotal biological processes as an aberrant transcriptional regulator, of which 16 genes were involved in lipid metabolism and highly enriched in glycerophospholipid metabolism.Conclusion: An effective and rapid protocol for obtaining duck primary hepatocytes was established, by which our findings manifested choline deficiency could induce the accumulation of lipid and result in aberrant AS events in hepatocytes, providing a novel insight into various AS in the metabolism role of choline.